{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T14:55:18Z","timestamp":1784127318449,"version":"3.55.0"},"reference-count":55,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T00:00:00Z","timestamp":1739404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["22-75-10140"],"award-info":[{"award-number":["22-75-10140"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The aim of this study was to examine the hepatoprotective activity of multicomponent mixtures of natural origin in the BALB\/C mouse model, with subacute liver failure (SALF) induced by the administration of toxin carbon tetrachloride (CCl4). The hepatoprotective activity of activated hydrolytic lignin (BP-Cx-1), humic acid peloids (HA), and isoflavones from kudzu Pueraria lobata roots (IFL) was evaluated using mass spectrometry (MS)-based omics technologies. Our MS-based approach revealed new insights into the molecular mechanisms of the hepatoprotective activity of multicomponent mixtures of natural origin. Significant differences were observed in the proteome and metabolome profiles of the urine and liver of BALB\/c mice with SALF between a control group with CCl4 administration, intact controls, and groups receiving potential hepatoprotectors of natural origin (BP-Cx-1, HA, IFL). Proteomic and metabolomics analyses demonstrated that among the hepatoprotectors, IFL possessed the highest hepatoprotective potential, which correlated well with the relative effectiveness of the drugs recorded during in vitro studies. These results correlate with the relative effectiveness of the drugs recorded in previous in vitro and in vivo studies. The leading IFL activity may be attributed to a higher content of active polyphenolic components compared to heterogeneous HA and BP-Cx-1. Enrichment with active components by fractionation is a direction that can be explored for developing hepatoprotective agents based on natural complex polyphenols.<\/jats:p>","DOI":"10.3390\/ijms26041604","type":"journal-article","created":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T10:36:58Z","timestamp":1739443018000},"page":"1604","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Hepatoprotective Activity of Nature-Derived Polyphenols Studied by Mass Spectrometry Based Multi-OMICS Approach"],"prefix":"10.3390","volume":"26","author":[{"given":"Alexander G.","family":"Brzhozovskiy","sequence":"first","affiliation":[{"name":"Project Center of Omics Technologies and Advanced Mass Spectrometry, 121205 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Savva D.","family":"Semenov","sequence":"additional","affiliation":[{"name":"Project Center of Omics Technologies and Advanced Mass Spectrometry, 121205 Moscow, Russia"},{"name":"Moscow Center for Advanced Studies, 123592 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3481-4606","authenticated-orcid":false,"given":"Alexander Y.","family":"Zherebker","sequence":"additional","affiliation":[{"name":"Project Center of Omics Technologies and Advanced Mass Spectrometry, 121205 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1515-9447","authenticated-orcid":false,"given":"Anna E.","family":"Bugrova","sequence":"additional","affiliation":[{"name":"Project Center of Omics Technologies and Advanced Mass Spectrometry, 121205 Moscow, Russia"},{"name":"Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3589-5871","authenticated-orcid":false,"given":"Maria N.","family":"Yurova","sequence":"additional","affiliation":[{"name":"Project Center of Omics Technologies and Advanced Mass Spectrometry, 121205 Moscow, Russia"},{"name":"N.N. Petrov National Medical Research Center of Oncology, 197758 Saint Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8734-5527","authenticated-orcid":false,"given":"Yury V.","family":"Zhernov","sequence":"additional","affiliation":[{"name":"Project Center of Omics Technologies and Advanced Mass Spectrometry, 121205 Moscow, Russia"},{"name":"A.N. Sysin Research Institute of Human Ecology and Environmental Hygiene, Centre for Strategic Planning and Management of Biomedical Health Risks of the Federal Medical and Biological Agency, 119435 Moscow, Russia"},{"name":"Department of General Hygiene, F. Erismann Institute of Public Health, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oxana A.","family":"Kovaleva","sequence":"additional","affiliation":[{"name":"Project Center of Omics Technologies and Advanced Mass Spectrometry, 121205 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander L.","family":"Semenov","sequence":"additional","affiliation":[{"name":"N.N. Petrov National Medical Research Center of Oncology, 197758 Saint Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry P.","family":"Abroskin","sequence":"additional","affiliation":[{"name":"Project Center of Omics Technologies and Advanced Mass Spectrometry, 121205 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stepan S.","family":"Kruglov","sequence":"additional","affiliation":[{"name":"N.N. Petrov National Medical Research Center of Oncology, 197758 Saint Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2426-9843","authenticated-orcid":false,"given":"Elena I.","family":"Fedoros","sequence":"additional","affiliation":[{"name":"N.N. Petrov National Medical Research Center of Oncology, 197758 Saint Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2238-3458","authenticated-orcid":false,"given":"Alexey S.","family":"Kononikhin","sequence":"additional","affiliation":[{"name":"Project Center of Omics Technologies and Advanced Mass Spectrometry, 121205 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeniy N.","family":"Nikolaev","sequence":"additional","affiliation":[{"name":"Project Center of Omics Technologies and Advanced Mass Spectrometry, 121205 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,13]]},"reference":[{"key":"ref_1","first-page":"349","article-title":"Oxidative\/Antioxidative Effects of Different Lignin Preparations on DNA in Hamster V79 Cells","volume":"47","year":"2000","journal-title":"Neoplasma"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.fuproc.2012.05.003","article-title":"Production of Phenols from Lignin via Depolymerization and Catalytic Cracking","volume":"108","author":"Yoshikawa","year":"2013","journal-title":"Fuel Process. 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