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The neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been extensively used in different animal species to develop chemical models of PD. This study aimed to evaluate the effects of acute exposure to MPTP (3 \u00d7 150 mg\/kg, intraperitoneally) on adult zebrafish by assessing the neurochemical, transcriptional, and motor changes associated with PD pathogenesis. MPTP treatment resulted in a significant decrease in brain catecholamines, including dopamine, norepinephrine, and normetanephrine. Additionally, a trend towards decreased levels of dopamine precursors (tyrosine and L-DOPA) and degradation products (3-MT and DOPAC) was also observed, although these changes were not statistically significant. Gene expression analysis showed the downregulation of dbh, while the expression of other genes involved in catecholamine metabolism (th1, th2, mao, comtb) and transport (slc6a3 and slc18a2) remained unaltered, suggesting a lack of dopaminergic neuron degeneration. Behavioral assessments revealed that MPTP-exposed zebrafish exhibited reduced motor activity, consistent with the observed decrease in dopamine levels. In contrast, the kinematic parameters of sharp turning were unaffected. A significant impairment in the sensorimotor gating of the ASR was detected in the MPTP-treated fish, consistent with psychosis. Despite dopamine depletion and behavioral impairments, the absence of neurodegeneration and some hallmark PD motor symptoms suggests limitations in the validity of this model for fully recapitulating PD pathology. Further studies are needed to refine the use of MPTP in zebrafish PD models.<\/jats:p>","DOI":"10.3390\/ijms26041674","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T03:41:47Z","timestamp":1739763707000},"page":"1674","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Motor and Non-Motor Effects of Acute MPTP in Adult Zebrafish: Insights into Parkinson\u2019s Disease"],"prefix":"10.3390","volume":"26","author":[{"given":"Niki","family":"Tagkalidou","sequence":"first","affiliation":[{"name":"Institute for Environmental Assessment and Water Research (IDAEA-CSIC), Jordi Girona, 18, 08034 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3089-8192","authenticated-orcid":false,"given":"Marija","family":"Stevanovi\u0107","sequence":"additional","affiliation":[{"name":"Institute of Pesticides and Environmental Protection, Banatska 31b, 11080 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Romero-Alfano","sequence":"additional","affiliation":[{"name":"Department of Analytical and Applied Chemistry, School of Engineering, Institut Qu\u00edmic de Sarri\u00e0-Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Axel","family":"Elizalde-Vel\u00e1zquez","sequence":"additional","affiliation":[{"name":"Laboratorio de Toxicolog\u00eda Ambiental, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma Del Estado de M\u00e9xico, Paseo Col\u00f3n Intersecci\u00f3n Paseo Tollocan, Colonia Residencial Col\u00f3n, Toluca CP 50120, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selene Elizabeth","family":"Herrera-V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Laboratorio de Toxicolog\u00eda Ambiental, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma Del Estado de M\u00e9xico, Paseo Col\u00f3n Intersecci\u00f3n Paseo Tollocan, Colonia Residencial Col\u00f3n, Toluca CP 50120, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7838-2027","authenticated-orcid":false,"given":"Eva","family":"Prats","sequence":"additional","affiliation":[{"name":"Research and Development Center (CID-CSIC), Jordi Girona, 18, 08034 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9073-6307","authenticated-orcid":false,"given":"Cristian","family":"G\u00f3mez-Canela","sequence":"additional","affiliation":[{"name":"Department of Analytical and Applied Chemistry, School of Engineering, Institut Qu\u00edmic de Sarri\u00e0-Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7248-3449","authenticated-orcid":false,"given":"Leobardo Manuel","family":"G\u00f3mez-Oliv\u00e1n","sequence":"additional","affiliation":[{"name":"Laboratorio de Toxicolog\u00eda Ambiental, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma Del Estado de M\u00e9xico, Paseo Col\u00f3n Intersecci\u00f3n Paseo Tollocan, Colonia Residencial Col\u00f3n, Toluca CP 50120, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5256-1641","authenticated-orcid":false,"given":"Demetrio","family":"Rald\u00faa","sequence":"additional","affiliation":[{"name":"Institute for Environmental Assessment and Water Research (IDAEA-CSIC), Jordi Girona, 18, 08034 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2112146","DOI":"10.1155\/2022\/2112146","article-title":"Effect of Different MPTP Administration Intervals on Mouse Models of Parkinson\u2019s Disease","volume":"2022","author":"Ma","year":"2022","journal-title":"Contrast Media Mol. 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