{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T21:47:34Z","timestamp":1788299254761,"version":"build-2803163510"},"reference-count":99,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,16]],"date-time":"2025-02-16T00:00:00Z","timestamp":1739664000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health","award":["HD100529"],"award-info":[{"award-number":["HD100529"]}]},{"name":"National Institutes of Health","award":["HD101852"],"award-info":[{"award-number":["HD101852"]}]},{"name":"National Institutes of Health","award":["HD109286"],"award-info":[{"award-number":["HD109286"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>We performed next-generation sequencing (NGS) on RNA from 19 paired leiomyoma (Lyo) and myometrium (Myo) specimens, stratified by race\/ethnicity (White: n = 7; Black: n = 12) and mediator complex subunit 12 (MED12) mutation status (mutated: n = 10; non-mutated: n = 9). Analysis identified 2,189 small non-coding RNAs (sncRNAs) with altered expression in Lyo compared to paired Myo (\u22651.5-fold change), including small nuclear RNAs (snRNAs), small nucleolar RNAs (snoRNAs), microRNAs (miRNAs), and PIWI-interacting RNAs (piRNAs). Among these, 17 sncRNAs showed differential expression in the MED12-mutated group versus Myo, while minimal changes were observed in the non-mutated group. Additionally, 31 sncRNAs displayed differential expression in Black women compared to White women. For validation, five novel miRNAs (miR-19a-3p, miR-99a-5p, miR-3196, miR-499a-5p, and miR-30d-3p) and five piRNAs (piR-009295, piR-020326, piR-020365, piR-006426, and piR-020485) were analyzed in 51 paired Lyo samples using qRT-PCR. Reduced expression of the selected sncRNAs was confirmed in Lyo versus Myo, with miR-19a-3p, miR-3196, miR-30d-3p, piR-006426, and piR-020485 linked to MED12 status, while miR-499a-5p and miR-30d-3p were associated with race\/ethnicity. These findings suggest that sncRNA dysregulation contributes to altered gene expression in Lyo, influenced by MED12 mutation and racial background.<\/jats:p>","DOI":"10.3390\/ijms26041688","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T03:41:47Z","timestamp":1739763707000},"page":"1688","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Differential Expression of Small Non-Coding RNAs in Uterine Leiomyomas"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1195-7054","authenticated-orcid":false,"given":"Tsai-Der","family":"Chuang","sequence":"first","affiliation":[{"name":"The Lundquist Institute for Biomedical Innovation, Torrance, CA 90502, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nhu","family":"Ton","sequence":"additional","affiliation":[{"name":"The Lundquist Institute for Biomedical Innovation, Torrance, CA 90502, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5899-0449","authenticated-orcid":false,"given":"Shawn","family":"Rysling","sequence":"additional","affiliation":[{"name":"The Lundquist Institute for Biomedical Innovation, Torrance, CA 90502, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Baghdasarian","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Harbor-UCLA Medical Center, Torrance, CA 90502, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4996-5762","authenticated-orcid":false,"given":"Omid","family":"Khorram","sequence":"additional","affiliation":[{"name":"The Lundquist Institute for Biomedical Innovation, Torrance, CA 90502, USA"},{"name":"Department of Obstetrics and Gynecology, Harbor-UCLA Medical Center, Torrance, CA 90502, USA"},{"name":"Department of Obstetrics and Gynecology, David Geffen School of Medicine at University of California, Los Angeles, CA 90095, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1177\/1933719114533728","article-title":"Uterine fibroids: Clinical manifestations and contemporary management","volume":"21","author":"Doherty","year":"2014","journal-title":"Reprod. 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