{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T23:43:25Z","timestamp":1776469405681,"version":"3.51.2"},"reference-count":38,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T00:00:00Z","timestamp":1739836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2023YFA0913904"],"award-info":[{"award-number":["2023YFA0913904"]}]},{"name":"National Key Research and Development Program of China","award":["XDB 0480102"],"award-info":[{"award-number":["XDB 0480102"]}]},{"name":"National Key Research and Development Program of China","award":["32271501"],"award-info":[{"award-number":["32271501"]}]},{"name":"National Key Research and Development Program of China","award":["ZDSYS20220303153551001"],"award-info":[{"award-number":["ZDSYS20220303153551001"]}]},{"name":"Strategic Priority Research Program of Chinese Academy of Sciences","award":["2023YFA0913904"],"award-info":[{"award-number":["2023YFA0913904"]}]},{"name":"Strategic Priority Research Program of Chinese Academy of Sciences","award":["XDB 0480102"],"award-info":[{"award-number":["XDB 0480102"]}]},{"name":"Strategic Priority Research Program of Chinese Academy of Sciences","award":["32271501"],"award-info":[{"award-number":["32271501"]}]},{"name":"Strategic Priority Research Program of Chinese Academy of Sciences","award":["ZDSYS20220303153551001"],"award-info":[{"award-number":["ZDSYS20220303153551001"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2023YFA0913904"],"award-info":[{"award-number":["2023YFA0913904"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["XDB 0480102"],"award-info":[{"award-number":["XDB 0480102"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32271501"],"award-info":[{"award-number":["32271501"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["ZDSYS20220303153551001"],"award-info":[{"award-number":["ZDSYS20220303153551001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shenzhen Science and Technology Plan Platform and Carrier Special Project","award":["2023YFA0913904"],"award-info":[{"award-number":["2023YFA0913904"]}]},{"name":"Shenzhen Science and Technology Plan Platform and Carrier Special Project","award":["XDB 0480102"],"award-info":[{"award-number":["XDB 0480102"]}]},{"name":"Shenzhen Science and Technology Plan Platform and Carrier Special Project","award":["32271501"],"award-info":[{"award-number":["32271501"]}]},{"name":"Shenzhen Science and Technology Plan Platform and Carrier Special Project","award":["ZDSYS20220303153551001"],"award-info":[{"award-number":["ZDSYS20220303153551001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>B7 homolog 3 (B7-H3) has emerged as a promising target for cancer therapy due to its high expression in various types of cancer cells. It not only regulates the activity of immune cells but also modulates the signal transduction and metabolism of cancer cells. However, the specific interaction partners of B7-H3 still remain unclear, limiting a comprehensive understanding of the precise role of B7-H3 in cancer progression. In this study, we report that B7-H3 can bind to resting Raji cells, stimulated THP-1 cells, and even PC3 prostate cancer cells through its IgV domain alone. Furthermore, to identify the potential interaction partners of B7-H3 on these cells, we adopted an ascorbate peroxidase 2 (APEX2)-based proximity labeling strategy, which revealed about 10 key potential interaction partners. Interestingly, our results suggest that CD45 could be a putative receptor for B7-H3 on Raji cells, while the epidermal growth factor receptor (EGFR) could closely interact with B7-H3 on PC3 cells. Based on further computational structure modeling studies, we show that B7-H3 can bind to the epidermal growth factor (EGF) binding pocket of EGFR\u2014surprisingly, with a stronger affinity than EGF itself. Overall, our study provides an effective approach to identifying B7-H3 interaction partners in both immune and cancer cell lines.<\/jats:p>","DOI":"10.3390\/ijms26041731","type":"journal-article","created":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T03:47:06Z","timestamp":1739850426000},"page":"1731","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Identification of the B7-H3 Interaction Partners Using a Proximity Labeling Strategy"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5526-142X","authenticated-orcid":false,"given":"Shujie","family":"Liao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiamin","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6987-5230","authenticated-orcid":false,"given":"Cecylia S.","family":"Lupala","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangcheng","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China"},{"name":"Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuefei","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.7150\/ijbs.41105","article-title":"B7-H3, a checkpoint molecule, as a target for cancer immunotherapy","volume":"16","author":"Yang","year":"2020","journal-title":"Int. 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