{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T04:49:14Z","timestamp":1773118154899,"version":"3.50.1"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004166","name":"Medical University of Warsaw, Poland","doi-asserted-by":"publisher","award":["WF1A\/1\/F\/GW\/N\/23"],"award-info":[{"award-number":["WF1A\/1\/F\/GW\/N\/23"]}],"id":[{"id":"10.13039\/501100004166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004166","name":"Medical University of Warsaw, Poland","doi-asserted-by":"publisher","award":["2023\/51\/B\/NZ9\/00305"],"award-info":[{"award-number":["2023\/51\/B\/NZ9\/00305"]}],"id":[{"id":"10.13039\/501100004166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Science Centre (Poland)","award":["WF1A\/1\/F\/GW\/N\/23"],"award-info":[{"award-number":["WF1A\/1\/F\/GW\/N\/23"]}]},{"name":"National Science Centre (Poland)","award":["2023\/51\/B\/NZ9\/00305"],"award-info":[{"award-number":["2023\/51\/B\/NZ9\/00305"]}]},{"name":"European Regional Development Fund","award":["WF1A\/1\/F\/GW\/N\/23"],"award-info":[{"award-number":["WF1A\/1\/F\/GW\/N\/23"]}]},{"name":"European Regional Development Fund","award":["2023\/51\/B\/NZ9\/00305"],"award-info":[{"award-number":["2023\/51\/B\/NZ9\/00305"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The extracts from fruits of Chaenomeles japonica (Thunb.) Lindl. ex Spach (CJE), Cornus mas L. (CME), and Hippopha\u0451 rhamnoides L. (HRE) are known inhibitors of a variety of eukaryotic hydrolases, engaged in the digestion of fats and polysaccharides. However, there are no data on their potential interaction with the bacterial hydrolases participating in the replication of microbial nucleic acids. This analysis predicted the interaction of the most abundant constituents of HRE, CJE, and CME with the bacterial nucleases. The analysis covered the molecular docking of isorhamnetin glycosides, procyanidins C1 and B2, epicatechin, loganic acid, and cornuside with bacterial enzymes (Escherichia coli endonuclease 1, colicin E9, and ribonuclease H; or Staphylococcus aureus thermonuclease and nuclease SbcCD). The suggested complexes have been subjected to molecular mechanics with generalized Born and surface area solvation (MM\/GBSA) calculations. The second aim was the in vitro evaluation of the influence of the CJE, HRE, and CME on the metabolic activity of bacterial biofilm of selected microbial strains, as well as fibroblasts (L929) and adenocarcinoma intestinal cells (Caco-2) toxicity. Among all extracts, CME showed the most relevant effect on the survival of planktonic cells and biofilm of E. coli and Pseudomonas aeruginosa. As a result of in silico studies, most virtual hits were predicted to inhibit the proteins under investigation, except for procyanidin C1. Further research on the direct interaction of phytochemicals and selected enzymes in vitro is required and challenged.<\/jats:p>","DOI":"10.3390\/ijms26041757","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T03:29:53Z","timestamp":1739935793000},"page":"1757","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Potential Impact of Edible Fruit Extracts on Bacterial Nucleases in Preliminary Research\u2014In Silico and In Vitro Insight"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5302-3797","authenticated-orcid":false,"given":"\u0141ukasz","family":"Szeleszczuk","sequence":"first","affiliation":[{"name":"Department of Organic and Physical Chemistry, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9520-3377","authenticated-orcid":false,"given":"Malwina","family":"Bro\u017cyna","sequence":"additional","affiliation":[{"name":"Platform for Unique Models Application, Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart\u0142omiej","family":"Dudek","sequence":"additional","affiliation":[{"name":"Platform for Unique Models Application, Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcin","family":"Czarnecki","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Liver Diseases and Acquired Immune Deficiencies, Wroclaw Medical University, Koszarowa 5, 51-149 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7559-8903","authenticated-orcid":false,"given":"Adam","family":"Junka","sequence":"additional","affiliation":[{"name":"Platform for Unique Models Application, Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8159-0174","authenticated-orcid":false,"given":"Monika E.","family":"Czerwi\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Pharmacogenomics, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland"},{"name":"Centre for Preclinical Research, Medical University of Warsaw, Banacha 1B, 02-097 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.5114\/pg.2018.76005","article-title":"The influence of antibiotics and dietary components on gut microbiota","volume":"13","author":"Junka","year":"2018","journal-title":"Gastroenterol. 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