{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T04:15:55Z","timestamp":1791778555615,"version":"4.3.4"},"reference-count":66,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Research and the Arts Baden-W\u00fcrttemberg (MWK)","award":["INST 35\/1503-1 FUGG"],"award-info":[{"award-number":["INST 35\/1503-1 FUGG"]}]},{"name":"German Research Foundation (DFG)","award":["INST 35\/1503-1 FUGG"],"award-info":[{"award-number":["INST 35\/1503-1 FUGG"]}]},{"name":"Heidelberg University","award":["INST 35\/1503-1 FUGG"],"award-info":[{"award-number":["INST 35\/1503-1 FUGG"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The human choroid plexus (CP) is the location of the blood\u2013cerebrospinal fluid (CSF) barrier (BCSFB). Whereas the epithelial cells of the CP mainly contribute to the formation of the BCSFB, the vessels of the CP are built by fenestrated endothelial cells. Still, the CP endothelium can contribute to barrier function. By ectopic expression of human telomerase reverse transcriptase (hTERT) in primary human CP endothelial cells (HCPEnCs), we recently generated and characterized immortalized HCPEnCs (iHCPEnCs). Here, we compared primary cells of the sixth passage (HCPEnCs p6) with a lower (p20) and a higher passage (p50) of iHCPEnCs by transcriptome analysis. A high concordance of HCPEnCs and both passages of iHCPEnCs was observed, as only small proportions of the transcripts examined were significantly altered. Differentially expressed genes (DEGs) were identified and assigned to potentially affected biological processes by gene set enrichment analysis (GSEA). Various components of the endothelial barrier-relevant Wnt signaling were detected in HCPEnCs and iHCPEnCs. Functional analysis of HCPEnCs and iHCPEnCs showed equal marginal activation of Wnt signaling, supporting the downregulation of \u03b2-catenin (CTNNB) signaling in CP endothelial cells, and a contribution to the barrier function by the CP endothelium was retained until passage 100 (p100) of iHCPEnCs. Overall, our data support the suitability of iHCPEnCs as an in vitro model of the CP endothelium over extended passages.<\/jats:p>","DOI":"10.3390\/ijms26041779","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T11:49:27Z","timestamp":1739965767000},"page":"1779","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Transcriptome and Functional Comparison of Primary and Immortalized Endothelial Cells of the Human Choroid Plexus at the Blood\u2013Cerebrospinal Fluid Barrier"],"prefix":"10.3390","volume":"26","author":[{"given":"Lea","family":"Denzer","sequence":"first","affiliation":[{"name":"Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"},{"name":"European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9418-6912","authenticated-orcid":false,"given":"Walter","family":"Muranyi","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"},{"name":"European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosanna","family":"Herold","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"},{"name":"European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolin","family":"Stump-Guthier","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"},{"name":"European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Regenerative Medicine, Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3596-6094","authenticated-orcid":false,"given":"Carsten","family":"Sticht","sequence":"additional","affiliation":[{"name":"Core Facility Next Generation Sequencing, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6779-1084","authenticated-orcid":false,"given":"Horst","family":"Schroten","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"},{"name":"European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1706-0259","authenticated-orcid":false,"given":"Christian","family":"Schwerk","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"},{"name":"European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Weichert","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"},{"name":"European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1172\/JCI27100","article-title":"Direct evidence for the role of caveolin-1 and caveolae in mechanotransduction and remodeling of blood vessels","volume":"116","author":"Yu","year":"2006","journal-title":"J. Clin. Investig."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/21541248.2016.1197872","article-title":"Leukocyte transendothelial migration: A local affair","volume":"8","author":"Schimmel","year":"2017","journal-title":"Small GTPases"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1038\/35025220","article-title":"Angiogenesis in cancer and other diseases","volume":"407","author":"Carmeliet","year":"2000","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/j.cell.2010.01.025","article-title":"Immunity, inflammation, and cancer","volume":"140","author":"Grivennikov","year":"2010","journal-title":"Cell"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1038\/nrm2889","article-title":"Breaching multiple barriers: Leukocyte motility through venular walls and the interstitium","volume":"11","author":"Nourshargh","year":"2010","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s11886-020-1275-9","article-title":"Diversity of Organism-Wide and Organ-Specific Endothelial Cells","volume":"22","author":"Przysinda","year":"2020","journal-title":"Curr. Cardiol. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"eaal2379","DOI":"10.1126\/science.aal2379","article-title":"Organotypic vasculature: From descriptive heterogeneity to functional pathophysiology","volume":"357","author":"Augustin","year":"2017","journal-title":"Science"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1161\/01.RES.0000255690.03436.ae","article-title":"Phenotypic heterogeneity of the endothelium: II. Representative vascular beds","volume":"100","author":"Aird","year":"2007","journal-title":"Circ. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1161\/01.RES.0000255691.76142.4a","article-title":"Phenotypic heterogeneity of the endothelium: I. Structure, function, and mechanisms","volume":"100","author":"Aird","year":"2007","journal-title":"Circ. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1055\/s-0030-1253447","article-title":"Morphological heterogeneity of endothelium","volume":"36","author":"Tse","year":"2010","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/s00441-023-03741-1","article-title":"The role of PLVAP in endothelial cells","volume":"392","author":"Denzer","year":"2023","journal-title":"Cell Tissue Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1146\/annurev.cellbio.20.010403.113126","article-title":"The Wnt signaling pathway in development and disease","volume":"20","author":"Logan","year":"2004","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.devcel.2009.06.016","article-title":"Wnt\/beta-catenin signaling: Components, mechanisms, and diseases","volume":"17","author":"MacDonald","year":"2009","journal-title":"Dev. Cell"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1161\/CIRCRESAHA.110.223750","article-title":"The role of wnt signaling in physiological and pathological angiogenesis","volume":"107","author":"Dejana","year":"2010","journal-title":"Circ. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1016\/j.devcel.2010.05.006","article-title":"The Wnt\/beta-catenin pathway modulates vascular remodeling and specification by upregulating Dll4\/Notch signaling","volume":"18","author":"Corada","year":"2010","journal-title":"Dev. Cell"},{"key":"ref_16","first-page":"cm1","article-title":"Wnt\/beta-catenin pathway","volume":"2005","author":"Moon","year":"2005","journal-title":"Sci. STKE"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.ydbio.2012.03.023","article-title":"In vivo Wnt signaling tracing through a transgenic biosensor fish reveals novel activity domains","volume":"366","author":"Moro","year":"2012","journal-title":"Dev. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5723","DOI":"10.1113\/JP275376","article-title":"Physiology and molecular biology of barrier mechanisms in the fetal and neonatal brain","volume":"596","author":"Saunders","year":"2018","journal-title":"J. Physiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3825","DOI":"10.1172\/JCI76431","article-title":"Canonical WNT signaling components in vascular development and barrier formation","volume":"124","author":"Zhou","year":"2014","journal-title":"J. Clin. Investig."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1126\/science.abc6108","article-title":"Identification of a choroid plexus vascular barrier closing during intestinal inflammation","volume":"374","author":"Carloni","year":"2021","journal-title":"Science"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"104383","DOI":"10.1016\/j.isci.2022.104383","article-title":"Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type in vitro model of the choroid plexus","volume":"25","author":"Muranyi","year":"2022","journal-title":"iScience"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1167\/iovs.08-1731","article-title":"Differential gene expression in Ndph-knockout mice in retinal development","volume":"50","author":"Schafer","year":"2009","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1083\/jcb.200806024","article-title":"Wnt\/beta-catenin signaling controls development of the blood-brain barrier","volume":"183","author":"Liebner","year":"2008","journal-title":"J. Cell Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6452","DOI":"10.1167\/iovs.10-7146","article-title":"Frizzled 4 is required for retinal angiogenesis and maintenance of the blood-retina barrier","volume":"52","author":"Paes","year":"2011","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1007\/s00441-014-1811-2","article-title":"Novel insights into the development and maintenance of the blood-brain barrier","volume":"355","author":"Engelhardt","year":"2014","journal-title":"Cell Tissue Res."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Laksitorini, M.D., Yathindranath, V., Xiong, W., Hombach-Klonisch, S., and Miller, D.W. (2019). Modulation of Wnt\/beta-catenin signaling promotes blood-brain barrier phenotype in cultured brain endothelial cells. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-56075-w"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3934","DOI":"10.1021\/jm049768a","article-title":"Substituted 3-imidazo[1,2-a]pyridin-3-yl- 4-(1,2,3,4-tetrahydro-[1,4]diazepino-[6,7,1-hi]indol-7-yl)pyrrole-2,5-diones as highly selective and potent inhibitors of glycogen synthase kinase-3","volume":"47","author":"Engler","year":"2004","journal-title":"J. Med. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1002\/path.2628","article-title":"Inactive Wnt\/beta-catenin pathway in conventional high-grade osteosarcoma","volume":"220","author":"Cai","year":"2010","journal-title":"J. Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/j.1749-0774.2005.tb00059.x","article-title":"Establishment and characterization of a human malignant choroids plexus papilloma cell line (HIBCPP)","volume":"18","author":"Ishiwata","year":"2005","journal-title":"Hum. Cell"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Schwerk, C., Papandreou, T., Schuhmann, D., Nickol, L., Borkowski, J., Steinmann, U., Quednau, N., Stump, C., Weiss, C., and Berger, J. (2012). Polar Invasion and Translocation of Neisseria meningitidis and Streptococcus suis in a Novel Human Model of the Blood-Cerebrospinal Fluid Barrier. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0030069"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s00401-009-0627-8","article-title":"Choroid plexus: Biology and pathology","volume":"119","author":"Wolburg","year":"2010","journal-title":"Acta Neuropathol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1093\/jnen\/nlv030","article-title":"The Choroid Plexus in Healthy and Diseased Brain","volume":"75","author":"Kaur","year":"2016","journal-title":"J. Neuropathol. Exp. Neurol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Liddelow, S.A. (2015). Development of the choroid plexus and blood-CSF barrier. Front. Neurosci., 9.","DOI":"10.3389\/fnins.2015.00032"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1038\/nrn3921","article-title":"Development and functions of the choroid plexus-cerebrospinal fluid system","volume":"16","author":"Lun","year":"2015","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.biochi.2020.11.015","article-title":"Fundamental insights into the interaction between telomerase\/TERT and intracellular signaling pathways","volume":"181","author":"Ghareghomi","year":"2021","journal-title":"Biochimie"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1038\/nrc2389","article-title":"Wnt signalling and its impact on development and cancer","volume":"8","author":"Klaus","year":"2008","journal-title":"Nat. Rev. Cancer"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1038\/s41467-021-27602-z","article-title":"Constitutive activation of canonical Wnt signaling disrupts choroid plexus epithelial fate","volume":"13","author":"Parichha","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"dev19205","DOI":"10.1242\/dev.192054","article-title":"MEIS-WNT5A axis regulates development of fourth ventricle choroid plexus","volume":"148","author":"Kaiser","year":"2021","journal-title":"Development"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e43257","DOI":"10.7554\/eLife.43257","article-title":"Beta-catenin signaling regulates barrier-specific gene expression in circumventricular organ and ocular vasculatures","volume":"8","author":"Wang","year":"2019","journal-title":"eLife"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3056","DOI":"10.1016\/j.cell.2021.04.003","article-title":"A cellular and spatial map of the choroid plexus across brain ventricles and ages","volume":"184","author":"Dani","year":"2021","journal-title":"Cell"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1186\/s12964-019-0444-1","article-title":"Induction of LEF1 by MYC activates the WNT pathway and maintains cell proliferation","volume":"17","author":"Hao","year":"2019","journal-title":"Cell Commun. Signal. CCS"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.cell.2008.01.052","article-title":"Rac1 activation controls nuclear localization of beta-catenin during canonical Wnt signaling","volume":"133","author":"Wu","year":"2008","journal-title":"Cell"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1093\/embo-reports\/kvf181","article-title":"The ins and outs of APC and beta-catenin nuclear transport","volume":"3","author":"Henderson","year":"2002","journal-title":"EMBO Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2882","DOI":"10.1073\/pnas.0307344101","article-title":"Nuclear-cytoplasmic shuttling of Axin regulates subcellular localization of beta-catenin","volume":"101","author":"Cong","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1083\/jcb.200502141","article-title":"RanBP3 enhances nuclear export of active (beta)-catenin independently of CRM1","volume":"171","author":"Hendriksen","year":"2005","journal-title":"J. Cell Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1096\/fj.04-3458fje","article-title":"Blood-brain barrier-specific properties of a human adult brain endothelial cell line","volume":"19","author":"Weksler","year":"2005","journal-title":"FASEB J."},{"key":"ref_47","first-page":"58","article-title":"Massive Analysis of cDNA Ends (MACE): Eine efektive RNA-Seq-Variante","volume":"23","author":"Rotter","year":"2017","journal-title":"Biospektrum Springer"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt Removes Adapter Sequences from High-Throughput Sequencing Reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_50","unstructured":"Pantaleoni, J., and Subtil, N. (2022, January 03). NVBIO 2015. Available online: https:\/\/nvlabs.github.io\/nvbio."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq\u2014A Python framework to work with high-throughput sequencing data","volume":"31","author":"Anders","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Love, M.I., Huber, W., and Anders, S. (2014). Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol., 15.","DOI":"10.1186\/s13059-014-0550-8"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3047","DOI":"10.1093\/bioinformatics\/btw354","article-title":"MultiQC: Summarize analysis results for multiple tools and samples in a single report","volume":"32","author":"Ewels","year":"2016","journal-title":"Bioinformatics"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1093\/bioinformatics\/btw313","article-title":"Complex heatmaps reveal patterns and correlations in multidimensional genomic data","volume":"32","author":"Gu","year":"2016","journal-title":"Bioinformatics"},{"key":"ref_55","unstructured":"Sergushichev, A.A. (2016). An algorithm for fast preranked gene set enrichment analysis using cumulative statistic calculation. bioRxiv."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto encyclopedia of genes and genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"D691","DOI":"10.1093\/nar\/gkq1018","article-title":"Reactome: A database of reactions, pathways and biological processes","volume":"39","author":"Croft","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"D258","DOI":"10.1093\/nar\/gkh036","article-title":"The Gene Ontology (GO) database and informatics resource","volume":"32","author":"Harris","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.neuron.2019.05.044","article-title":"Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion Cells","volume":"103","author":"Bray","year":"2019","journal-title":"Neuron"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e02156","DOI":"10.1016\/j.heliyon.2019.e02156","article-title":"Selection of best reference genes for qRT-PCR analysis of human neural stem cells preconditioned with hypoxia or baicalein-enriched fraction extracted from Oroxylum indicum medicinal plant","volume":"5","author":"Kang","year":"2019","journal-title":"Heliyon"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s10529-017-2493-0","article-title":"Dexamethasone downregulates SIRT1 and IL6 and upregulates EDN1 genes in stem cells derived from gingivae via the AGE\/RAGE pathway","volume":"40","author":"Lee","year":"2018","journal-title":"Biotechnol. Lett."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"7932","DOI":"10.3168\/jds.2012-6383","article-title":"Evaluation of internal reference genes for quantitative expression analysis by real-time reverse transcription-PCR in somatic cells from goat milk","volume":"96","author":"Modesto","year":"2013","journal-title":"J. Dairy Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"110517","DOI":"10.18632\/oncotarget.22806","article-title":"GATA3-induced vWF upregulation in the lung adenocarcinoma vasculature","volume":"8","author":"Xu","year":"2017","journal-title":"Oncotarget"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"712","DOI":"10.21037\/jtd.2019.12.106","article-title":"Knockdown of PLAT enhances the anticancer effect of gefitinib in non-small cell lung cancer","volume":"12","author":"Yan","year":"2020","journal-title":"J. Thorac. Dis."},{"key":"ref_66","first-page":"1875","article-title":"Knockdown of SERPINE1 reverses resistance of triple-negative breast cancer to paclitaxel via suppression of VEGFA","volume":"44","author":"Zhang","year":"2020","journal-title":"Oncol. Rep."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/4\/1779\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:38:08Z","timestamp":1760027888000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/4\/1779"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,19]]},"references-count":66,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["ijms26041779"],"URL":"https:\/\/doi.org\/10.3390\/ijms26041779","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,19]]}}}