{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:44:57Z","timestamp":1790142297726,"version":"4.0.1"},"reference-count":51,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:00:00Z","timestamp":1740096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"UAMI-I-CA-136 Animal Biology Reproduction","award":["426152"],"award-info":[{"award-number":["426152"]}]},{"name":"Academic Body (C.A.B.R.A)","award":["426152"],"award-info":[{"award-number":["426152"]}]},{"name":"PRODEP-SEP","award":["426152"],"award-info":[{"award-number":["426152"]}]},{"DOI":"10.13039\/501100003141","name":"Conahcyt (M\u00e9xico)","doi-asserted-by":"publisher","award":["426152"],"award-info":[{"award-number":["426152"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Infertility is increasingly recognized as being closely linked to obesity in humans. The successful production of fertile spermatozoa requires adequate spermatogenesis within the testis and proper spermatozoa maturation through the epididymis. This study aimed to evaluate the impact of body adiposity on male fertility, focusing on sperm parameters, epididymal sperm maturation, and sperm capacitation in Wistar rats. Male rats were randomized into three dietary groups over four weeks: a control group receiving less than 4% lard, regular chow, a 10% lard group, and a 60% lard group. Following dietary interventions, fertility tests were conducted across the groups. The epididymis was dissected into caput, corpus, and cauda regions to assess sperm concentration, vitality capacitation, carbohydrate distribution, tyrosine phosphorylation, and phosphatidylserine levels. Additionally, serum testosterone levels were measured to evaluate hormonal influences on fertility. The rats subjected to high-fat diets leading to overweight and obesity exhibited significant alterations in fertility. These changes were characterized by impaired epididymal sperm maturation, as evidenced by lower testosterone levels, decreased sperm viability, and capacitation. Furthermore, increased adiposity was associated with a lack of asymmetry in the plasma membrane, alteration in carbohydrate distribution, and changes in tyrosine phosphorylation. This study underscores the adverse effects of high-fat diets on male fertility, particularly through mechanisms affecting sperm maturation in the epididymis. The evidence suggests that obesity-induced alterations in sperm parameters and hormonal profiles may contribute to reduced fertility in male rats, which could have implications for understanding similar human processes.<\/jats:p>","DOI":"10.3390\/ijms26051850","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T06:39:07Z","timestamp":1740119947000},"page":"1850","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["High-Fat Diets Disturb Rat Epididymal Sperm Maturation"],"prefix":"10.3390","volume":"26","author":[{"given":"Lorena","family":"Ruiz-Valderrama","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda de la Reproducci\u00f3n, Universidad Aut\u00f3noma Metropolitana, Iztapalapa, Ciudad de M\u00e9xico 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Edwin","family":"Mendoza-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Doctorado en Biolog\u00eda Experimental, Universidad Aut\u00f3noma Metropolitana, Iztapalapa, Ciudad de M\u00e9xico 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4426-0587","authenticated-orcid":false,"given":"Ernesto","family":"Rodr\u00edguez-Tob\u00f3n","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda de la Reproducci\u00f3n, Universidad Aut\u00f3noma Metropolitana, Iztapalapa, Ciudad de M\u00e9xico 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Arrieta-Cruz","sequence":"additional","affiliation":[{"name":"Departamento de Investigaci\u00f3n B\u00e1sica, Instituto Nacional de Geriatr\u00eda, Magdalena Contreras, Ciudad de M\u00e9xico 10200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3792-9094","authenticated-orcid":false,"given":"Humberto","family":"Gonz\u00e1lez-M\u00e1rquez","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias de la Salud, Universidad Aut\u00f3noma Metropolitana, Iztapalapa, Ciudad de M\u00e9xico 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo Arturo","family":"Salame-M\u00e9ndez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda de la Reproducci\u00f3n, Universidad Aut\u00f3noma Metropolitana, Iztapalapa, Ciudad de M\u00e9xico 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosario","family":"Tarrag\u00f3-Castellanos","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda de la Reproducci\u00f3n, Universidad Aut\u00f3noma Metropolitana, Iztapalapa, Ciudad de M\u00e9xico 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0186-7338","authenticated-orcid":false,"given":"Edith","family":"Cort\u00e9s-Barberena","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias de la Salud, Universidad Aut\u00f3noma Metropolitana, Iztapalapa, Ciudad de M\u00e9xico 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2201-7316","authenticated-orcid":false,"given":"Ahiezer","family":"Rodr\u00edguez-Tob\u00f3n","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda de la Reproducci\u00f3n, Universidad Aut\u00f3noma Metropolitana, Iztapalapa, Ciudad de M\u00e9xico 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2534-9351","authenticated-orcid":false,"given":"Edith","family":"Arenas-R\u00edos","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda de la Reproducci\u00f3n, Universidad Aut\u00f3noma Metropolitana, Iztapalapa, Ciudad de M\u00e9xico 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,21]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2010). 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