{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T08:47:23Z","timestamp":1782204443997,"version":"3.54.5"},"reference-count":58,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T00:00:00Z","timestamp":1740355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wroc\u0142aw University of Environmental and Life Sciences"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Staphylococcus pettenkoferi (S. pettenkoferi) is a rare opportunistic bacterium not commonly found in healthy individuals or animals. S. pettenkoferi has increasing clinical significance in both veterinary and human medicine due to its multidrug resistance and biofilm-forming ability. This study analyzed 12 isolates of S. pettenkoferi collected from humans and cats and identified them using matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA and partial rpoB gene sequencing. All of the S. pettenkoferi were phenotypically resistant to penicillin, and almost all (except one human strain) were resistant to methicillin. Antibiotic susceptibility testing revealed a high prevalence of multidrug resistance in all human strains with frequent resistance to \u03b2-lactams, macrolides, and tetracyclines. A comparative analysis of human and feline isolates indicated the presence of shared resistance genes such as blaZ, mecA, and ermA. Biofilm production varied across isolates, with more potent biofilm formation abilities observed at elevated temperatures (39 \u00b0C) and time (48 h). These findings underscore the potential zoonotic risks of S. pettenkoferi and its role in managing multidrug-resistant infections.<\/jats:p>","DOI":"10.3390\/ijms26051948","type":"journal-article","created":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T06:47:17Z","timestamp":1740379637000},"page":"1948","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Comparison of Staphylococcus pettenkoferi Isolated from Human Clinical Cases and Cat Carriers Regarding Antibiotic Susceptibility and Biofilm Production"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7663-4312","authenticated-orcid":false,"given":"Karolina","family":"Bierowiec","sequence":"first","affiliation":[{"name":"Department of Epizootiology and Clinic of Birds and Exotic Animals, Division of Infectious Diseases and Veterinary Administration, The Faculty of Veterinary Medicine in Wroc\u0142aw, Wroc\u0142aw University of Environmental and Life Sciences, 50-366 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0610-9775","authenticated-orcid":false,"given":"Ashley","family":"Delmar","sequence":"additional","affiliation":[{"name":"EZA Student Science Club, Department of Epizootiology and Clinic of Birds and Exotic Animals, Division of Infectious Diseases and Veterinary Administration, The Faculty of Veterinary Medicine in Wroc\u0142aw, Wroc\u0142aw University of Environmental and Life Sciences, 50-366 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Karwa\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Epizootiology and Clinic of Birds and Exotic Animals, Division of Infectious Diseases and Veterinary Administration, The Faculty of Veterinary Medicine in Wroc\u0142aw, Wroc\u0142aw University of Environmental and Life Sciences, 50-366 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Siedlecka","sequence":"additional","affiliation":[{"name":"Department of Epizootiology and Clinic of Birds and Exotic Animals, Division of Infectious Diseases and Veterinary Administration, The Faculty of Veterinary Medicine in Wroc\u0142aw, Wroc\u0142aw University of Environmental and Life Sciences, 50-366 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Kumala-\u0106wik\u0142a","sequence":"additional","affiliation":[{"name":"Department of Epizootiology and Clinic of Birds and Exotic Animals, Division of Infectious Diseases and Veterinary Administration, The Faculty of Veterinary Medicine in Wroc\u0142aw, Wroc\u0142aw University of Environmental and Life Sciences, 50-366 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Ksi\u0105\u017cczyk","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Biological Sciences, University of Wroc\u0142aw, 51-148 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2722-9596","authenticated-orcid":false,"given":"Katarzyna","family":"Kapczy\u0144ska","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0732-8893(02)00399-1","article-title":"Staphylococcus pettenkoferi, a novel staphylococcal species isolated from clinical specimens","volume":"43","author":"Rinder","year":"2002","journal-title":"Diagn. 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