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However, there has been little exploration regarding the effects of CUM on ulcerative colitis (UC). Therefore, this study aimed to investigate the beneficial effects of CUM on alleviating dextran sulfate sodium (DSS)-induced UC in mice and to explore the potential underlying mechanisms. Here, the effect of CUM on UC was analyzed using a DSS-induced colitis mouse model (n = 9), the results of which indicated a decrease in disease activity index (DAI) in DSS-induced UC mice. Furthermore, CUM alleviated colon shortening, minimized intestinal tissue damage, and preserved intestinal tight junction proteins (Claudin-3, Occludin, and ZO-1). CUM reduced the level of pro-inflammatory cytokines (IL-1\u03b2, IL-6, and TNF-\u03b1), inhibited the activation of the NF-\u0138B, MAPK and PPAR\u03b3 signaling pathways, and decreased the level of oxidative mediators (MPO, SOD and MDA) in the colon of UC mice. Additionally, it mitigated the dysbiosis of intestinal microbiota in UC mice by increasing the abundance of Prevotellaceae and Lactobacillus while decreasing the abundance of Bacteroidaceae and Enterobacteriaceae. CUM alleviated the decrease in short-chain fatty acids (SCFAs) content in the colon of UC mice. The above results provide a scientific basis for CUM, as a natural supplement, to restore the balance of the gut inflammatory microbiota and promote gut health.<\/jats:p>","DOI":"10.3390\/ijms26051993","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T06:13:18Z","timestamp":1740463998000},"page":"1993","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Candida utilis Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice via NF-\u03baB\/MAPK Suppression and Gut Microbiota Modulation"],"prefix":"10.3390","volume":"26","author":[{"given":"Rongxin","family":"Zang","sequence":"first","affiliation":[{"name":"College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730100, China"},{"name":"Engineering Research Center of Key Technology and Industrialization of Cell-Based Vaccine, Ministry of Education, Lanzhou 730030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhouliang","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huihao","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Northwest Minzu University, Lanzhou 730030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenyan","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730100, China"},{"name":"Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Northwest Minzu University, Lanzhou 730030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fazheng","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaodong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongwei","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730100, China"},{"name":"Gansu Tech Innovation Center of Animal Cell, Northwest Minzu University, Lanzhou 730030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1038\/s41467-024-45473-y","article-title":"Porphyromonas gingivalis aggravates colitis via a gut microbiota-linoleic acid metabolism-Th17\/Treg cell balance axis","volume":"15","author":"Jia","year":"2024","journal-title":"Nat. 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BMC Microbiol., 24.","DOI":"10.1186\/s12866-024-03216-5"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/5\/1993\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:42:07Z","timestamp":1760028127000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/5\/1993"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,25]]},"references-count":35,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["ijms26051993"],"URL":"https:\/\/doi.org\/10.3390\/ijms26051993","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,25]]}}}