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This is mainly due to endothelial dysregulation, which causes plasma leakage and hemorrhage. However, the etiology of DV-induced endothelial dysregulation remains incompletely understood. To identify the potential mechanisms of endothelial dysregulation caused by DV, the effects of conditioned media from Dengue virus (CMDV) on the mechanics and transcriptional profile of the endothelial cells were examined using permeability assays, atomic force microscopy, In-Cell Western blot and in silico transcriptomics. Exposure of HMEC-1 cells to the CMDV increased endothelial permeability and cellular stiffness. It also induced the expression of the key proteins associated with endothelial-to-mesenchymal transition (EndMT). These data support the notion that the DV promotes endothelial dysfunction by triggering transcriptional programs that compromise the endothelial barrier function. Understanding the molecular mechanisms underlying DV-induced endothelial dysregulation is crucial for developing targeted therapeutic strategies to mitigate the severe outcomes associated with dengue infection.<\/jats:p>","DOI":"10.3390\/ijms26052139","type":"journal-article","created":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T02:48:37Z","timestamp":1740710917000},"page":"2139","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Characterization of the Temporal Dynamics of the Endothelial\u2013Mesenchymal-like Transition Induced by Soluble Factors from Dengue Virus Infection in Microvascular Endothelial Cells"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7274-4469","authenticated-orcid":false,"given":"Jenny Paola","family":"Alfaro-Garc\u00eda","sequence":"first","affiliation":[{"name":"Grupo Medicina de Translaci\u00f3n\u2014Facultad de Medicina, Universidad de Antioquia, Medell\u00edn 050010, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Alberto","family":"Orozco-Casta\u00f1o","sequence":"additional","affiliation":[{"name":"Grupo de Biolog\u00eda del C\u00e1ncer, Instituto Nacional de Cancerolog\u00eda, Bogot\u00e1 111511, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4909-988X","authenticated-orcid":false,"given":"Juli\u00e1n Andr\u00e9s","family":"S\u00e1nchez-Rend\u00f3n","sequence":"additional","affiliation":[{"name":"Grupo de Coloides\u2014Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia, Medell\u00edn 050010, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herley Fernando","family":"Casanova-Y\u00e9pes","sequence":"additional","affiliation":[{"name":"Grupo de Coloides\u2014Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia, Medell\u00edn 050010, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5943-3220","authenticated-orcid":false,"given":"Miguel","family":"Vicente-Manzanares","sequence":"additional","affiliation":[{"name":"Molecular Mechanisms Program, Centro de Investigaci\u00f3n del C\u00e1ncer, Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer, Consejo Superior de Investigaciones Cient\u00edficas (CSIC)\u2014University of Salamanca, 37007 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7453-2569","authenticated-orcid":false,"given":"Juan Carlos","family":"Gallego-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Grupo Medicina de Translaci\u00f3n\u2014Facultad de Medicina, Universidad de Antioquia, Medell\u00edn 050010, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"520","DOI":"10.14423\/SMJ.0000000000001152","article-title":"Arboviruses and Their Vectors","volume":"113","author":"Madewell","year":"2020","journal-title":"South. 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