{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:11:55Z","timestamp":1790802715100,"version":"4.1.0"},"reference-count":103,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Chongqing Technology Innovation and Application Development Special Key Project","award":["2023TIAD-KPX0067"],"award-info":[{"award-number":["2023TIAD-KPX0067"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Vitellogenesis in fish represents a critical phase of oogenesis, significantly influencing the nutritional provisioning for oocyte maturation and subsequent offspring development. However, research on the physiological mechanisms governing vitellogenesis at the single-cell level remains limited. In this study, we performed single-nucleus RNA sequencing (snRNA-seq) on the ovaries of Sichuan bream (Sinibrama\u00a0taeniatus). We first identified six distinct cell types (germ cells, follicular cells, immune cells, stromal cells, endothelial cells, and epithelial cells) in the ovaries based on typical functional marker genes. Subsequently, we reconstructed the developmental trajectory of germ cells using pseudotime analysis, which describes the transcriptional dynamics of germ cells at various developmental stages. Additionally, we identified transcription factors (TFs) specific to germ cells that exhibit high activity at each developmental stage. Furthermore, we analyzed the genetic functional heterogeneity of germ cells and follicular cells at different developmental stages to elucidate their contributions to vitellogenesis. Finally, cell interaction analysis revealed that germ cells communicate with somatic cells or with each other via multiple receptors and ligands to regulate growth, development, and yolk acquisition. These findings enhance our understanding of the physiological mechanisms underlying vitellogenesis in fish, providing a theoretical foundation for regulating ovarian development in farmed fish.<\/jats:p>","DOI":"10.3390\/ijms26052265","type":"journal-article","created":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T03:26:21Z","timestamp":1741058781000},"page":"2265","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Characterizing the Ovarian Cytogenetic Dynamics of Sichuan Bream (Sinibrama\u00a0taeniatus) During Vitellogenesis at a Single-Cell Resolution"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-6408-7437","authenticated-orcid":false,"given":"Zhe","family":"Zhao","sequence":"first","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Southwest University, Chongqing 401329, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shixia","family":"Huang","sequence":"additional","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Southwest University, Chongqing 401329, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qilin","family":"Feng","sequence":"additional","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Southwest University, Chongqing 401329, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Peng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), School of Life Sciences, Southwest University, Chongqing 400715, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Southwest University, Chongqing 401329, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijian","family":"Wang","sequence":"additional","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Southwest University, Chongqing 401329, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.ygcen.2015.01.025","article-title":"Ovarian yolk formation in fishes: Molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins","volume":"221","author":"Hiramatsu","year":"2015","journal-title":"Gen. 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