{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T00:44:50Z","timestamp":1793148290424,"version":"4.5.0"},"reference-count":164,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T00:00:00Z","timestamp":1741132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Engineering and Physical Sciences Research Council (EPSRC)","award":["EP\/T517823\/1"],"award-info":[{"award-number":["EP\/T517823\/1"]}]},{"name":"Engineering and Physical Sciences Research Council (EPSRC)","award":["EP\/W524347\/1"],"award-info":[{"award-number":["EP\/W524347\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Myocardial infarction remains a leading cause of death worldwide due to the heart\u2019s limited regenerative capability and the current lack of viable therapeutic solutions. Therefore, there is an urgent need to develop effective treatment options to restore cardiac function after a heart attack. Stem cell-derived cardiac cells have been extensively utilised in cardiac tissue regeneration studies. However, the use of Matrigel as a substrate for the culture and maturation of these cells has been a major limitation for the translation of this research into clinical application. Hydrogels are emerging as a promising system to overcome this problem. They are biocompatible and can provide stem cells with a supportive scaffold that mimics the extracellular matrix, which is essential for repairing damaged tissue in the myocardium after an infarction. Thus, hydrogels provide an alternative and reproducible option in addressing myocardial infarction due to their unique potential therapeutic benefits. This review explores the different types of natural and synthetic polymers used to create hydrogels and their various delivery methods, the most common being via injection and cardiac patches and other applications such as bioprinting. Many challenges remain before hydrogels can be used in a clinical setting, but they hold great promise for the future of cardiac tissue regeneration.<\/jats:p>","DOI":"10.3390\/ijms26052309","type":"journal-article","created":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T05:54:38Z","timestamp":1741154078000},"page":"2309","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Hydrogels for Cardiac Tissue Regeneration: Current and Future Developments"],"prefix":"10.3390","volume":"26","author":[{"given":"Sonja","family":"Holme","sequence":"first","affiliation":[{"name":"Division of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7637-4135","authenticated-orcid":false,"given":"Stephen M.","family":"Richardson","sequence":"additional","affiliation":[{"name":"Division of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0443-4562","authenticated-orcid":false,"given":"Jordi","family":"Bella","sequence":"additional","affiliation":[{"name":"Division of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Pinali","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9NT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"256","DOI":"10.21037\/atm.2016.06.33","article-title":"Epidemiology of coronary heart disease and acute coronary syndrome","volume":"4","author":"Leischik","year":"2016","journal-title":"Ann. Transl. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2350","DOI":"10.1016\/j.jacc.2023.11.007","article-title":"Global Burden of Cardiovascular Diseases and Risks, 1990\u20132022","volume":"82","author":"Mensah","year":"2023","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_3","unstructured":"Global Burden of Disease Collaborative Network (2024). Global Burden of Disease Study 2021 (GBD 2021), Institute for Health Metrics and Evaluation (IHME). Available online: http:\/\/www.healthdata.org\/research-analysis\/library\/global-burden-disease-2021-findings-gbd-2021-study."},{"key":"ref_4","unstructured":"World Health Organization (2023, December 12). UN Decade of Healthy Ageing: Plan of Action 2021\u20132030. Available online: https:\/\/cdn.who.int\/media\/docs\/default-source\/decade-of-healthy-ageing\/decade-proposal-final-apr2020-en.pdf."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2343","DOI":"10.1016\/j.jacc.2023.11.003","article-title":"A Heart-Healthy and Stroke-Free World: Using Data to Inform Global Action","volume":"82","author":"Mensah","year":"2023","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_6","unstructured":"Institute of Medicine Committee on Social Security Cardiovascular Disability Criteria (2010). Cardiovascular Disability: Updating the Social Security Listings, National Academies Press."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/ije\/dyq165","article-title":"World Health Organization definition of myocardial infarction: 2008-09 revision","volume":"40","author":"Mendis","year":"2011","journal-title":"Int. J. Epidemiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/j.mcna.2007.03.005","article-title":"Pathophysiology of Acute Myocardial Infarction","volume":"91","author":"Burke","year":"2007","journal-title":"Med. Clin. N. Am."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1093\/eurheartj\/ehv071","article-title":"Myocardial infarction: Mechanisms, diagnosis, and complications","volume":"36","year":"2015","journal-title":"Eur. Heart J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e16","DOI":"10.1161\/CIR.0000000000000985","article-title":"Mechanical Complications of Acute Myocardial Infarction: A Scientific Statement from the American Heart Association","volume":"144","author":"Damluji","year":"2021","journal-title":"Circulation"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1093\/eurheartj\/ehu505","article-title":"Cardiovascular risk in post-myocardial infarction patients: Nationwide real world data demonstrate the importance of a long-term perspective","volume":"36","author":"Jernberg","year":"2015","journal-title":"Eur. Heart J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1007\/s12013-015-0553-4","article-title":"Myocardial Infarction: Symptoms and Treatments","volume":"72","author":"Lu","year":"2015","journal-title":"Cell Biochem. Biophys."},{"key":"ref_13","unstructured":"Ahmad, M., Mehta, P., Reddivari, A.K.R., and Mungee, S. (2024). Percutaneous Coronary Intervention. StatPearls, StatPearls Publishing LLC."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Mousaei Ghasroldasht, M., Seok, J., Park, H.S., Liakath Ali, F.B., and Al-Hendy, A. (2022). Stem Cell Therapy: From Idea to Clinical Practice. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23052850"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1056\/NEJM200106073442303","article-title":"Evidence that human cardiac myocytes divide after myocardial infarction","volume":"344","author":"Beltrami","year":"2001","journal-title":"N. Engl. J. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1126\/science.1164680","article-title":"Evidence for cardiomyocyte renewal in humans","volume":"324","author":"Bergmann","year":"2009","journal-title":"Science"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1002\/med.21839","article-title":"Combining stem cells in myocardial infarction: The road to superior repair?","volume":"42","author":"Evens","year":"2022","journal-title":"Med. Res. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1152\/physiol.00036.2016","article-title":"Modeling Physiological Events in 2D vs. 3D Cell Culture","volume":"32","author":"Duval","year":"2017","journal-title":"Physiology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.4252\/wjsc.v11.i12.1065","article-title":"Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling","volume":"11","author":"Chaicharoenaudomrung","year":"2019","journal-title":"World J. Stem Cells"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Gisone, I., Cecchettini, A., Ceccherini, E., Persiani, E., Morales, M.A., and Vozzi, F. (2022). Cardiac tissue engineering: Multiple approaches and potential applications. Front. Bioeng. Biotechnol., 10.","DOI":"10.3389\/fbioe.2022.980393"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Oh, S., and Choo, A.B.H. (2011). Stem Cells. Comprehensive Biotechnology, Academic Press. [2nd ed.].","DOI":"10.1016\/B978-0-08-088504-9.00038-6"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Augustine, R., Dan, P., Hasan, A., Khalaf, I.M., Prasad, P., Ghosal, K., Gentile, C., McClements, L., and Maureira, P. (2021). Stem cell-based approaches in cardiac tissue engineering: Controlling the microenvironment for autologous cells. Biomed. Pharmacother., 138.","DOI":"10.1016\/j.biopha.2021.111425"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1111\/j.1469-7580.2008.00878.x","article-title":"Tissue engineering: Strategies, stem cells and scaffolds","volume":"213","author":"Howard","year":"2008","journal-title":"J. Anat."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Vishwakarma, A., Sharpe, P., Shi, S., and Ramalingam, M. (2015). Chapter 18: Embryonic Versus Adult Stem Cells. Stem Cell Biology and Tissue Engineering in Dental Sciences, Academic Press.","DOI":"10.1016\/B978-0-12-397157-9.00001-1"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Akter, F. (2016). Chapter 6-Cardiovascular Tissue Engineering. Tissue Engineering Made Easy, Academic Press.","DOI":"10.1016\/B978-0-12-805361-4.00006-0"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1186\/s13287-020-01648-0","article-title":"Mending a broken heart: Current strategies and limitations of cell-based therapy","volume":"11","author":"Liew","year":"2020","journal-title":"Stem Cell Res. Ther."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1007\/s12015-013-9448-1","article-title":"Adult stem cells and cardiac regeneration","volume":"9","author":"Turksen","year":"2013","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Lee, S.J., Yoo, J.J., and Atala, A. (2016). Chapter 13-Tissue-Derived Matrices. In Situ Tissue Regeneration, Academic Press.","DOI":"10.1016\/B978-0-12-802225-2.00001-5"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1126\/science.282.5391.1145","article-title":"Embryonic stem cell lines derived from human blastocysts","volume":"282","author":"Thomson","year":"1998","journal-title":"Science"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1111\/1755-5922.12032","article-title":"Clinical application of adult stem cells for therapy for cardiac disease","volume":"31","author":"Ghodsizad","year":"2013","journal-title":"Cardiovasc. Ther."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.cell.2006.07.024","article-title":"Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors","volume":"126","author":"Takahashi","year":"2006","journal-title":"Cell"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/j.cell.2007.11.019","article-title":"Induction of pluripotent stem cells from adult human fibroblasts by defined factors","volume":"131","author":"Takahashi","year":"2007","journal-title":"Cell"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s00441-021-03414-x","article-title":"Recent progress in induced pluripotent stem cell-derived 3D cultures for cardiac regeneration","volume":"384","author":"Wang","year":"2021","journal-title":"Cell Tissue Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1016\/j.athoracsur.2003.09.081","article-title":"Cellular cardiomyoplasty: Clinical application","volume":"77","author":"Chachques","year":"2004","journal-title":"Ann. Thorac. Surg."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"121","DOI":"10.2217\/rme.09.74","article-title":"Stem cell paracrine actions and tissue regeneration","volume":"5","author":"Baraniak","year":"2010","journal-title":"Regen. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/S0140-6736(00)03617-5","article-title":"Myoblast transplantation for heart failure","volume":"357","author":"Scorsin","year":"2001","journal-title":"Lancet"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Muqresh, M.A., Harbieh, I., and Haider, K.H. (2023). Myogenic Cardiac Regeneration: Clinical Studies Using Skeletal Myoblasts. Handbook of Stem Cell Applications, Springer.","DOI":"10.1007\/978-981-99-0846-2_54-1"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2369","DOI":"10.1001\/jama.2012.25321","article-title":"Comparison of allogeneic vs autologous bone marrow\u2013derived mesenchymal stem cells delivered by transendocardial injection in patients with ischemic cardiomyopathy: The POSEIDON randomized trial","volume":"308","author":"Hare","year":"2012","journal-title":"JAMA"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1038\/s41569-018-0036-6","article-title":"Therapeutic approaches for cardiac regeneration and repair","volume":"15","author":"Hashimoto","year":"2018","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1093\/eurheartj\/ehv136","article-title":"Bone marrow-derived mesenchymal stromal cell treatment in patients with severe ischaemic heart failure: A randomized placebo-controlled trial (MSC-HF trial)","volume":"36","author":"Mathiasen","year":"2015","journal-title":"Eur. Heart J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1038\/nature13233","article-title":"Human embryonic-stem-cell-derived cardiomyocytes regenerate non-human primate hearts","volume":"510","author":"Chong","year":"2014","journal-title":"Nature"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1161\/CIRCRESAHA.117.311578","article-title":"Lack of Remuscularization Following Transplantation of Human Embryonic Stem Cell-Derived Cardiovascular Progenitor Cells in Infarcted Nonhuman Primates","volume":"122","author":"Zhu","year":"2018","journal-title":"Circ. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/nbt.4162","article-title":"Human embryonic stem cell-derived cardiomyocytes restore function in infarcted hearts of non-human primates","volume":"36","author":"Liu","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.yjmcc.2023.03.009","article-title":"Cellular reprogramming of fibroblasts in heart regeneration","volume":"180","author":"Chi","year":"2023","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e1629","DOI":"10.1002\/wsbm.1629","article-title":"Two decades of heart regeneration research: Cardiomyocyte proliferation and beyond","volume":"16","author":"Huang","year":"2024","journal-title":"WIREs Mech. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1038\/nature19815","article-title":"Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts","volume":"538","author":"Shiba","year":"2016","journal-title":"Nature"},{"key":"ref_47","first-page":"286","article-title":"Maturation of induced pluripotent stem cell-derived cardiomyocytes and its therapeutic effect on myocardial infarction in mouse","volume":"20","author":"Wu","year":"2023","journal-title":"Bioact. Mater."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1097\/TP.0000000000002384","article-title":"Transplantation of Human-induced Pluripotent Stem Cell-derived Cardiomyocytes Is Superior to Somatic Stem Cell Therapy for Restoring Cardiac Function and Oxygen Consumption in a Porcine Model of Myocardial Infarction","volume":"103","author":"Shida","year":"2019","journal-title":"Transplantation"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"S29","DOI":"10.1161\/CIRCULATIONAHA.111.084343","article-title":"Feasibility, safety, and therapeutic efficacy of human induced pluripotent stem cell-derived cardiomyocyte sheets in a porcine ischemic cardiomyopathy model","volume":"126","author":"Kawamura","year":"2012","journal-title":"Circulation"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.cardfail.2022.10.433","article-title":"Cardiac Regenerative Therapy Using Human Pluripotent Stem Cells for Heart Failure: A State-of-the-Art Review","volume":"29","author":"Kishino","year":"2023","journal-title":"J. Card. Fail."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Wang, D., Chen, M., Yang, B., Zhang, F., and Cao, K. (2011). Intramyocardial transplantation of undifferentiated rat induced pluripotent stem cells causes tumorigenesis in the heart. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0019012"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1161\/CIRCRESAHA.114.302533","article-title":"Myocardial extracellular matrix: An ever-changing and diverse entity","volume":"114","author":"Rienks","year":"2014","journal-title":"Circ. Res."},{"key":"ref_53","unstructured":"Rama Sashank, M., Joseph Orgel, P.R.O., and Zvi, L. (2021). Chapter 2: Extracellular Matrix in Cardiac Tissue Mechanics and Physiology: Role of Collagen Accumulation. Extracellular Matrix, IntechOpen."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Song, R., and Zhang, L. (2020). Cardiac ECM: Its Epigenetic Regulation and Role in Heart Development and Repair. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21228610"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/s41578-020-0199-8","article-title":"Synthetic alternatives to Matrigel","volume":"5","author":"Aisenbrey","year":"2020","journal-title":"Nat. Rev. Mater."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Funaki, M., and Janmey, P.A. (2017). Chapter 23-Technologies to Engineer Cell Substrate Mechanics in Hydrogels. Biology and Engineering of Stem Cell Niches, Elsevier.","DOI":"10.1016\/B978-0-12-802734-9.00023-8"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1093\/ilar.49.3.310","article-title":"From bench to cageside: Risk assessment for rodent pathogen contamination of cells and biologics","volume":"49","author":"Peterson","year":"2008","journal-title":"ILAR J."},{"key":"ref_58","first-page":"105","article-title":"Chapter Four-Nanotechnology in wastewater treatment","volume":"Volume 99","author":"Turan","year":"2022","journal-title":"Comprehensive Analytical Chemistry"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1039\/C9PY01021A","article-title":"Hydrogel scaffolds for tissue engineering: The importance of polymer choice","volume":"11","author":"Spicer","year":"2020","journal-title":"Polym. Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s43088-021-00172-1","article-title":"Hydrogel based tissue engineering and its future applications in personalized disease modeling and regenerative therapy","volume":"11","author":"Chaudhary","year":"2022","journal-title":"Beni Suef Univ. J. Basic Appl. Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4337","DOI":"10.1016\/S0142-9612(03)00340-5","article-title":"Hydrogels for tissue engineering: Scaffold design variables and applications","volume":"24","author":"Drury","year":"2003","journal-title":"Biomaterials"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Gomez-Florit, M., Pardo, A., Domingues, R.M.A., Gra\u00e7a, A.L., Babo, P.S., Reis, R.L., and Gomes, M.E. (2020). Natural-Based Hydrogels for Tissue Engineering Applications. Molecules, 25.","DOI":"10.3390\/molecules25245858"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"50376","DOI":"10.1002\/app.50376","article-title":"Synthetic hydrogels: Synthesis, novel trends, and applications","volume":"138","author":"Madihally","year":"2021","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"485","DOI":"10.34297\/AJBSR.2021.11.001682","article-title":"A review of hydrogels, their properties and applications in medicine","volume":"11","author":"Chamkouri","year":"2021","journal-title":"Am. J. Biomed. Sci. Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1039\/C8BM01246F","article-title":"Advances in crosslinking strategies of biomedical hydrogels","volume":"7","author":"Hu","year":"2019","journal-title":"Biomater. Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.jare.2013.07.006","article-title":"Hydrogel: Preparation, characterization, and applications: A review","volume":"6","author":"Ahmed","year":"2015","journal-title":"J. Adv. Res."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Stojkov, G., Niyazov, Z., Picchioni, F., and Bose, R.K. (2021). Relationship between Structure and Rheology of Hydrogels for Various Applications. Gels, 7.","DOI":"10.3390\/gels7040255"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Hama, R., Ulziibayar, A., Reinhardt, J.W., Watanabe, T., Kelly, J., and Shinoka, T. (2023). Recent Developments in Biopolymer-Based Hydrogels for Tissue Engineering Applications. Biomolecules, 13.","DOI":"10.3390\/biom13020280"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"33523","DOI":"10.1021\/acsami.8b10064","article-title":"Ultratough, Self-Healing, and Tissue-Adhesive Hydrogel for Wound Dressing","volume":"10","author":"Chen","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1039\/C7BM00780A","article-title":"Supramolecular polymeric biomaterials","volume":"6","author":"Mann","year":"2018","journal-title":"Biomater. Sci."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Pan, Z., Ye, H., and Wu, D. (2021). Recent advances on polymeric hydrogels as wound dressings. APL Bioeng., 5.","DOI":"10.1063\/5.0038364"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.ejpb.2007.02.025","article-title":"Thermoresponsive hydrogels in biomedical applications","volume":"68","author":"Klouda","year":"2008","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0144-8617(88)90001-X","article-title":"Alginates","volume":"8","author":"Gacesa","year":"1988","journal-title":"Carbohydr. Polym."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"112468","DOI":"10.1039\/C6RA24006B","article-title":"Preparation and properties of polyacrylamide\/polyvinyl alcohol physical double network hydrogel","volume":"6","author":"Zhang","year":"2016","journal-title":"RSC Adv."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1023\/A:1008983215833","article-title":"Effect of chitosan and dextran on the properties of poly (vinyl alcohol) hydrogels","volume":"10","author":"Cascone","year":"1999","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Nasution, H., Harahap, H., Dalimunthe, N.F., Ginting, M.H.S., Jaafar, M., Tan, O.O.H., Aruan, H.K., and Herfananda, A.L. (2022). Hydrogel and Effects of Crosslinking Agent on Cellulose-Based Hydrogels: A Review. Gels, 8.","DOI":"10.3390\/gels8090568"},{"key":"ref_77","unstructured":"Conde, J. (2018). Chapter 15-Hydrogels as a New Platform to Recapitulate the Tumor Microenvironment. Handbook of Nanomaterials for Cancer Theranostics, Elsevier."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"10238","DOI":"10.1021\/acs.jafc.3c01162","article-title":"A Review of the Mechanism, Properties, and Applications of Hydrogels Prepared by Enzymatic Cross-linking","volume":"71","author":"Li","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1007\/s00253-012-4569-z","article-title":"Enzyme-catalyzed protein crosslinking","volume":"97","author":"Heck","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.eurpolymj.2017.01.027","article-title":"A review of the designs and prominent biomedical advances of natural and synthetic hydrogel formulations","volume":"88","author":"Gyles","year":"2017","journal-title":"Eur. Polym. J."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1002\/bip.22486","article-title":"Review collagen-based biomaterials for wound healing","volume":"101","author":"Chattopadhyay","year":"2014","journal-title":"Biopolymers"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1160\/TH04-08-0522","article-title":"Myocardial extra-cellular matrix and its regulation by metalloproteinases and their inhibitors","volume":"93","author":"Kassiri","year":"2005","journal-title":"Thromb. Haemost."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Andreazza, R., Morales, A., Pieniz, S., and Labidi, J. (2023). Gelatin-Based Hydrogels: Potential Biomaterials for Remediation. Polymers, 15.","DOI":"10.3390\/polym15041026"},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Sun, M., Sun, X., Wang, Z., Guo, S., Yu, G., and Yang, H. (2018). Synthesis and Properties of Gelatin Methacryloyl (GelMA) Hydrogels and Their Recent Applications in Load-Bearing Tissue. Polymers, 10.","DOI":"10.3390\/polym10111290"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Lierova, A., Kasparova, J., Filipova, A., Cizkova, J., Pekarova, L., Korecka, L., Mannova, N., Bilkova, Z., and Sinkorova, Z. (2022). Hyaluronic Acid: Known for Almost a Century, but Still in Vogue. Pharmaceutics, 14.","DOI":"10.3390\/pharmaceutics14040838"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"3280","DOI":"10.1039\/c2sm06463d","article-title":"Hyaluronic acid-based hydrogels: From a natural polysaccharide to complex networks","volume":"8","author":"Xu","year":"2012","journal-title":"Soft Matter"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s10856-019-6318-7","article-title":"Overview of natural hydrogels for regenerative medicine applications","volume":"30","author":"Catoira","year":"2019","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"101452","DOI":"10.1016\/j.progpolymsci.2021.101452","article-title":"Collagen blended with natural polymers: Recent advances and trends","volume":"122","author":"Sionkowska","year":"2021","journal-title":"Prog. Polym. Sci."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1500122","DOI":"10.1002\/advs.201500122","article-title":"Injectable Hydrogels for Cardiac Tissue Repair after Myocardial Infarction","volume":"2","author":"Hasan","year":"2015","journal-title":"Adv. Sci."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.jacc.2004.04.040","article-title":"Injectable fibrin scaffold improves cell transplant survival, reduces infarct expansion, and induces neovasculature formation in ischemic myocardium","volume":"44","author":"Christman","year":"2004","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"221","DOI":"10.31083\/j.rcm.2019.04.534","article-title":"Application of injectable hydrogels for cardiac stem cell therapy and tissue engineering","volume":"20","author":"Alagarsamy","year":"2019","journal-title":"Rev. Cardiovasc. Med."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Khanna, A., Zamani, M., and Huang, N.F. (2021). Extracellular Matrix-Based Biomaterials for Cardiovascular Tissue Engineering. J. Cardiovasc. Dev. Dis., 8.","DOI":"10.3390\/jcdd8110137"},{"key":"ref_93","unstructured":"Dua, K., Mehta, M., de Jesus Andreoli Pinto, T., Pont, L.G., Williams, K.A., and Rathbone, M.J. (2021). Chapter 28: Advancements on microparticles-based drug delivery systems for cancer therapy. Advanced Drug Delivery Systems in the Management of Cancer, Academic Press."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"2056","DOI":"10.1038\/nprot.2012.127","article-title":"Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior","volume":"7","author":"Fischer","year":"2012","journal-title":"Nat. Protoc."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Patel, L., Worch, J.C., Dove, A.P., and Gehmlich, K. (2023). The Utilisation of Hydrogels for iPSC-Cardiomyocyte Research. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24129995"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"4639","DOI":"10.1016\/j.biomaterials.2010.02.044","article-title":"Bioactive modification of poly (ethylene glycol) hydrogels for tissue engineering","volume":"31","author":"Zhu","year":"2010","journal-title":"Biomaterials"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"740","DOI":"10.3390\/polym3020740","article-title":"Hydrogels for Cardiac Tissue Engineering","volume":"3","author":"Li","year":"2011","journal-title":"Polymers"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.ejpb.2014.07.005","article-title":"Polyethylene glycol (PEG)\u2013Poly (N-isopropylacrylamide) (PNIPAAm) based thermosensitive injectable hydrogels for biomedical applications","volume":"88","author":"Alexander","year":"2014","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1016\/j.jmbbm.2011.04.005","article-title":"PVA hydrogel properties for biomedical application","volume":"4","author":"Jiang","year":"2011","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.ijpharm.2016.10.061","article-title":"Hydrogel based approaches for cardiac tissue engineering","volume":"523","author":"Saludas","year":"2017","journal-title":"Int. J. Pharm."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.tibtech.2016.01.002","article-title":"Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair","volume":"34","author":"Klotz","year":"2016","journal-title":"Trends Biotechnol."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.biomaterials.2015.08.045","article-title":"Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels","volume":"73","author":"Yue","year":"2015","journal-title":"Biomaterials"},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Pe\u00f1a, B., Laughter, M., Jett, S., Rowland, T.J., Taylor, M.R.G., Mestroni, L., and Park, D. (2018). Injectable Hydrogels for Cardiac Tissue Engineering. Macromol. Biosci., 18.","DOI":"10.1002\/mabi.201800079"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"2036","DOI":"10.1039\/D2TB02019J","article-title":"Hydrogels with electrically conductive nanomaterials for biomedical applications","volume":"11","author":"Kougkolos","year":"2023","journal-title":"J. Mater. Chem. B"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.addr.2019.06.001","article-title":"Electrically conductive nanomaterials for cardiac tissue engineering","volume":"144","author":"Ashtari","year":"2019","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Li, X.-P., Qu, K.-Y., Zhou, B., Zhang, F., Wang, Y.-Y., Abodunrin, O.D., Zhu, Z., and Huang, N.-P. (2021). Electrical stimulation of neonatal rat cardiomyocytes using conductive polydopamine-reduced graphene oxide-hybrid hydrogels for constructing cardiac microtissues. Colloids Surf. B Biointerfaces, 205.","DOI":"10.1016\/j.colsurfb.2021.111844"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.addr.2020.10.005","article-title":"Synthetic peptide hydrogels as 3D scaffolds for tissue engineering","volume":"160","author":"Ding","year":"2020","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.addr.2015.04.023","article-title":"Self-assembling peptide-based delivery of therapeutics for myocardial infarction","volume":"96","author":"French","year":"2016","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_109","first-page":"120","article-title":"Self-assemble peptide biomaterials and their biomedical applications","volume":"4","author":"Chen","year":"2019","journal-title":"Bioact. Mater."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"24459","DOI":"10.1021\/acsami.8b08659","article-title":"Folic Acid Derived Hydrogel Enhances the Survival and Promotes Therapeutic Efficacy of iPS Cells for Acute Myocardial Infarction","volume":"10","author":"Li","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_111","doi-asserted-by":"crossref","unstructured":"Burgess, K.A., Frati, C., Meade, K., Gao, J., Castillo Diaz, L., Madeddu, D., Graiani, G., Cavalli, S., Miller, A.F., and Oceandy, D. (2021). Functionalised peptide hydrogel for the delivery of cardiac progenitor cells. Mater. Sci. Eng. C Mater. Biol. Appl., 119.","DOI":"10.1016\/j.msec.2020.111539"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S0142-9612(01)00099-0","article-title":"Control of self-assembling oligopeptide matrix formation through systematic variation of amino acid sequence","volume":"23","author":"Caplan","year":"2002","journal-title":"Biomaterials"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1163\/156856202320176493","article-title":"Effects of systematic variation of amino acid sequence on the mechanical properties of a self-assembling, oligopeptide biomaterial","volume":"13","author":"Caplan","year":"2002","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/pj.2012.216","article-title":"Hydrogel scaffolds composed of genetically synthesized self-assembling peptides for three-dimensional cell culture","volume":"45","author":"Mie","year":"2013","journal-title":"Polym. J."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1007\/s12015-024-10721-7","article-title":"Delivery of Stem Cells and BMP-2 With Functionalized Self-Assembling Peptide Enhances Regeneration of Infarcted Myocardium","volume":"20","author":"Guo","year":"2024","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"20200153","DOI":"10.1002\/VIW.20200153","article-title":"Recent fabrications and applications of cardiac patch in myocardial infarction treatment","volume":"3","author":"Li","year":"2022","journal-title":"View"},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.msec.2010.12.017","article-title":"Biomimetic material strategies for cardiac tissue engineering","volume":"31","author":"Prabhakaran","year":"2011","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"88F","DOI":"10.1016\/S0002-9149(02)03343-X","article-title":"Surgical therapy in advanced heart failure","volume":"91","author":"Vitali","year":"2003","journal-title":"Am. J. Cardiol."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.biomaterials.2016.07.035","article-title":"Functional engineered human cardiac patches prepared from nature\u2019s platform improve heart function after acute myocardial infarction","volume":"105","author":"Wang","year":"2016","journal-title":"Biomaterials"},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1038\/nature07040","article-title":"Stem-cell-based therapy and lessons from the heart","volume":"453","author":"Passier","year":"2008","journal-title":"Nature"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/j.stem.2014.11.009","article-title":"Cardiac repair in a porcine model of acute myocardial infarction with human induced pluripotent stem cell-derived cardiovascular cells","volume":"15","author":"Ye","year":"2014","journal-title":"Cell Stem Cell"},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"943","DOI":"10.4070\/kcj.2021.0291","article-title":"Biomaterials-based Approaches for Cardiac Regeneration","volume":"51","author":"Vasu","year":"2021","journal-title":"Korean Circ. J."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"1712","DOI":"10.1161\/CIRCULATIONAHA.117.030785","article-title":"Large Cardiac Muscle Patches Engineered from Human Induced-Pluripotent Stem Cell-Derived Cardiac Cells Improve Recovery from Myocardial Infarction in Swine","volume":"137","author":"Gao","year":"2018","journal-title":"Circulation"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"33088","DOI":"10.1021\/acsami.8b13571","article-title":"Cardiac Stem Cell Patch Integrated with Microengineered Blood Vessels Promotes Cardiomyocyte Proliferation and Neovascularization after Acute Myocardial Infarction","volume":"10","author":"Su","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"4256","DOI":"10.1021\/acsbiomaterials.0c00418","article-title":"Delivering More of an Injectable Human Recombinant Collagen III Hydrogel Does Not Improve Its Therapeutic Efficacy for Treating Myocardial Infarction","volume":"6","author":"Pupkaite","year":"2020","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1007\/s11897-023-00630-0","article-title":"Hydrogels for Cardiac Restorative Support: Relevance of Gelation Mechanisms for Prospective Clinical Use","volume":"20","author":"Vetter","year":"2023","journal-title":"Curr. Heart Fail. Rep."},{"key":"ref_127","doi-asserted-by":"crossref","unstructured":"Li, P., Hu, J., Wang, J., Zhang, J., Wang, L., and Zhang, C. (2023). The Role of Hydrogel in Cardiac Repair and Regeneration for Myocardial Infarction: Recent Advances and Future Perspectives. Bioengineering, 10.","DOI":"10.3390\/bioengineering10020165"},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1161\/CIRCINTERVENTIONS.114.001478","article-title":"Intracoronary Delivery of Injectable Bioabsorbable Scaffold (IK-5001) to Treat Left Ventricular Remodeling After ST-Elevation Myocardial Infarction","volume":"7","author":"Frey","year":"2014","journal-title":"Circ. Cardiovasc. Interv."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"2297","DOI":"10.1093\/eurheartj\/ehv259","article-title":"A prospective comparison of alginate-hydrogel with standard medical therapy to determine impact on functional capacity and clinical outcomes in patients with advanced heart failure (AUGMENT-HF trial)","volume":"36","author":"Anker","year":"2015","journal-title":"Eur. Heart J."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"2300029","DOI":"10.1002\/adhm.202300029","article-title":"Advances in Injectable Hydrogel Strategies for Heart Failure Treatment","volume":"12","author":"He","year":"2023","journal-title":"Adv. Healthc. Mater."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"e004058","DOI":"10.1161\/CIRCINTERVENTIONS.116.004058","article-title":"Injectable Shear-Thinning Hydrogels for Minimally Invasive Delivery to Infarcted Myocardium to Limit Left Ventricular Remodeling","volume":"9","author":"Rodell","year":"2016","journal-title":"Circ. Cardiovasc. Interv."},{"key":"ref_132","doi-asserted-by":"crossref","unstructured":"Silva-Pedrosa, R., Salgado, A.J., and Ferreira, P.E. (2023). Revolutionizing Disease Modeling: The Emergence of Organoids in Cellular Systems. Cells, 12.","DOI":"10.3390\/cells12060930"},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"e274","DOI":"10.1002\/mco2.274","article-title":"Organoids: The current status and biomedical applications","volume":"4","author":"Yang","year":"2023","journal-title":"MedComm"},{"key":"ref_134","doi-asserted-by":"crossref","unstructured":"Xuan, W., Tipparaju, S.M., and Ashraf, M. (2022). Transformational Applications of Human Cardiac Organoids in Cardiovascular Diseases. Front. Cell Dev. Biol., 10.","DOI":"10.3389\/fcell.2022.936084"},{"key":"ref_135","doi-asserted-by":"crossref","unstructured":"Sahara, M. (2023). Recent Advances in Generation of In Vitro Cardiac Organoids. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24076244"},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/s41536-021-00140-4","article-title":"Next generation of heart regenerative therapies: Progress and promise of cardiac tissue engineering","volume":"6","author":"Tenreiro","year":"2021","journal-title":"NPJ Regen. Med."},{"key":"ref_137","doi-asserted-by":"crossref","unstructured":"Sun, Z., Zhao, J., Leung, E., Flandes-Iparraguirre, M., Vernon, M., Silberstein, J., De-Juan-Pardo, E.M., and Jansen, S. (2023). Three-Dimensional Bioprinting in Cardiovascular Disease: Current Status and Future Directions. Biomolecules, 13.","DOI":"10.3390\/biom13081180"},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1039\/C7BM00765E","article-title":"Bioinks for 3D bioprinting: An overview","volume":"6","author":"Inci","year":"2018","journal-title":"Biomater. Sci."},{"key":"ref_139","first-page":"1388","article-title":"Advances in 3D bioprinting technology for cardiac tissue engineering and regeneration","volume":"6","author":"Liu","year":"2021","journal-title":"Bioact. Mater."},{"key":"ref_140","doi-asserted-by":"crossref","unstructured":"Fatimi, A., Okoro, O.V., Podstawczyk, D., Siminska-Stanny, J., and Shavandi, A. (2022). Natural Hydrogel-Based Bio-Inks for 3D Bioprinting in Tissue Engineering: A Review. Gels, 8.","DOI":"10.3390\/gels8030179"},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"7948","DOI":"10.7150\/thno.61621","article-title":"3D bioprinting in cardiac tissue engineering","volume":"11","author":"Wang","year":"2021","journal-title":"Theranostics"},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1007\/s42242-022-00192-5","article-title":"Droplet-based bioprinting enables the fabrication of cell\u2013hydrogel\u2013microfibre composite tissue precursors","volume":"5","author":"Kotlarz","year":"2022","journal-title":"Bio-Des. Manuf."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.biomaterials.2016.06.012","article-title":"A comprehensive review on droplet-based bioprinting: Past, present and future","volume":"102","author":"Gudapati","year":"2016","journal-title":"Biomaterials"},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"76","DOI":"10.25289\/ML.2021.10.2.76","article-title":"An Overview of Laser-assisted Bioprinting (LAB) in Tissue Engineering Applications","volume":"10","author":"Ventura","year":"2021","journal-title":"Med. Lasers"},{"key":"ref_145","doi-asserted-by":"crossref","unstructured":"Wu, C.A., Zhu, Y., and Woo, Y.J. (2023). Advances in 3D Bioprinting: Techniques, Applications, and Future Directions for Cardiac Tissue Engineering. Bioengineering, 10.","DOI":"10.3390\/bioengineering10070842"},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"106039","DOI":"10.1016\/j.isci.2023.106039","article-title":"Stereolithography apparatus and digital light processing-based 3D bioprinting for tissue fabrication","volume":"26","author":"Li","year":"2023","journal-title":"iScience"},{"key":"ref_147","doi-asserted-by":"crossref","unstructured":"Boularaoui, S., Al Hussein, G., Khan, K.A., Christoforou, N., and Stefanini, C. (2020). An overview of extrusion-based bioprinting with a focus on induced shear stress and its effect on cell viability. Bioprinting, 20.","DOI":"10.1016\/j.bprint.2020.e00093"},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.matpr.2022.09.010","article-title":"Design, fabrication, and testing of a low-cost extrusion based 3D bioprinter for thermo-sensitive and light sensitive hydrogels","volume":"70","author":"Ferreira","year":"2022","journal-title":"Mater. Today Proc."},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1089\/ten.tec.2022.0214","article-title":"Optimization of Freeform Reversible Embedding of Suspended Hydrogel Microspheres for Substantially Improved Three-Dimensional Bioprinting Capabilities","volume":"29","author":"Wu","year":"2023","journal-title":"Tissue Eng. Part. C Methods"},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1126\/science.aav9051","article-title":"3D bioprinting of collagen to rebuild components of the human heart","volume":"365","author":"Lee","year":"2019","journal-title":"Science"},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.yjmcc.2023.12.009","article-title":"Stem cell-based therapy in cardiac repair after myocardial infarction: Promise, challenges, and future directions","volume":"188","author":"Yan","year":"2024","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_152","doi-asserted-by":"crossref","unstructured":"Xu, Q., Xiao, Z., Yang, Q., Yu, T., Deng, X., Chen, N., Huang, Y., Wang, L., Guo, J., and Wang, J. (2024). Hydrogel-based cardiac repair and regeneration function in the treatment of myocardial infarction. Mater. Today Bio, 25.","DOI":"10.1016\/j.mtbio.2024.100978"},{"key":"ref_153","first-page":"3904","article-title":"Biofabrication of natural hydrogels for cardiac, neural, and bone Tissue engineering Applications","volume":"6","author":"Elkhoury","year":"2021","journal-title":"Bioact. Mater."},{"key":"ref_154","doi-asserted-by":"crossref","unstructured":"Zhao, L., Zhou, Y., Zhang, J., Liang, H., Chen, X., and Tan, H. (2023). Natural Polymer-Based Hydrogels: From Polymer to Biomedical Applications. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15102514"},{"key":"ref_155","doi-asserted-by":"crossref","unstructured":"Ho, T.-C., Chang, C.-C., Chan, H.-P., Chung, T.-W., Shu, C.-W., Chuang, K.-P., Duh, T.-H., Yang, M.-H., and Tyan, Y.-C. (2022). Hydrogels: Properties and Applications in Biomedicine. Molecules, 27.","DOI":"10.3390\/molecules27092902"},{"key":"ref_156","doi-asserted-by":"crossref","unstructured":"Salehi, S., Naghib, S.M., Garshasbi, H.R., Ghorbanzadeh, S., and Zhang, W. (2023). Smart stimuli-responsive injectable gels and hydrogels for drug delivery and tissue engineering applications: A review. Front. Bioeng. Biotechnol., 11.","DOI":"10.3389\/fbioe.2023.1104126"},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1039\/C1SM06513K","article-title":"Shear-thinning hydrogels for biomedical applications","volume":"8","author":"Guvendiren","year":"2012","journal-title":"Soft Matter"},{"key":"ref_158","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.yjmcc.2022.04.020","article-title":"Minimally invasive delivery of a hydrogel-based exosome patch to prevent heart failure","volume":"169","author":"Cheng","year":"2022","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1038\/s41551-021-00796-9","article-title":"Injectable and conductive cardiac patches repair infarcted myocardium in rats and minipigs","volume":"5","author":"Wang","year":"2021","journal-title":"Nat. Biomed. Eng."},{"key":"ref_160","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.jacbts.2020.12.006","article-title":"3-Dimensional Bioprinting of Cardiovascular Tissues: Emerging Technology","volume":"6","author":"Sung","year":"2021","journal-title":"JACC Basic Transl. Sci."},{"key":"ref_161","doi-asserted-by":"crossref","unstructured":"Gardin, C., Ferroni, L., Latremouille, C., Chachques, J.C., Mitre\u010di\u0107, D., and Zavan, B. (2020). Recent Applications of Three Dimensional Printing in Cardiovascular Medicine. Cells, 9.","DOI":"10.3390\/cells9030742"},{"key":"ref_162","doi-asserted-by":"crossref","first-page":"e1900554","DOI":"10.1002\/adhm.201900554","article-title":"3D Bioprinting: The Emergence of Programmable Biodesign","volume":"9","author":"Begum","year":"2020","journal-title":"Adv. Healthc. Mater."},{"key":"ref_163","doi-asserted-by":"crossref","first-page":"105374","DOI":"10.1016\/j.reactfunctpolym.2022.105374","article-title":"4D bioprinting of smart polymers for biomedical applications: Recent progress, challenges, and future perspectives","volume":"179","author":"Arif","year":"2022","journal-title":"React. Funct. Polym."},{"key":"ref_164","doi-asserted-by":"crossref","unstructured":"Kabirian, F., Mela, P., and Heying, R. (2022). 4D Printing Applications in the Development of Smart Cardiovascular Implants. Front. Bioeng. 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