{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:48:07Z","timestamp":1791337687559,"version":"4.3.1"},"reference-count":33,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T00:00:00Z","timestamp":1741132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011789","name":"Science and Technology Department of Jilin Province","doi-asserted-by":"publisher","award":["20240404035ZP"],"award-info":[{"award-number":["20240404035ZP"]}],"id":[{"id":"10.13039\/501100011789","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011789","name":"Science and Technology Department of Jilin Province","doi-asserted-by":"publisher","award":["2024CX275"],"award-info":[{"award-number":["2024CX275"]}],"id":[{"id":"10.13039\/501100011789","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004032","name":"Graduate Innovation Fund of Jilin University","doi-asserted-by":"publisher","award":["20240404035ZP"],"award-info":[{"award-number":["20240404035ZP"]}],"id":[{"id":"10.13039\/501100004032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004032","name":"Graduate Innovation Fund of Jilin University","doi-asserted-by":"publisher","award":["2024CX275"],"award-info":[{"award-number":["2024CX275"]}],"id":[{"id":"10.13039\/501100004032","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Acute lung injury (ALI) is a life-threatening condition triggered by pneumonia, viral infections, or physical trauma. It manifests clinically as progressive respiratory failure and refractory hypoxemia. Using a lipopolysaccharide (LPS)-induced acute lung injury mouse model, we demonstrated that amniotic mesenchymal stem cells (AMSCs) exhibit robust reparative and anti-inflammatory properties. Our analysis encompassed inflammatory mediators; histological damage; tight junction integrity; epithelial\u2013mesenchymal transition (EMT); and the TGF-\u03b2\/Smad, TLR4\/NF-\u03baB\/MAPK, pyroptosis, and apoptosis signaling pathways. Our key results demonstrated that in ALI-afflicted mice, AMSCs exhibited targeted pulmonary tropism, homing in on injured alveolar regions, where they restored the morphology and functionality of damaged tissues and organelles, re-established lung barrier function, and attenuated the aberrantly activated TLR4\/NF-\u03baB\/MAPK and TGF-\u03b2\/Smad pathways associated with inflammation. These coordinated mechanisms contributed to pyroptosis, apoptosis, and fibrosis suppression. In conclusion, AMSCs mitigated the inflammatory injury process in ALI mice through multiple mechanisms, thereby supporting the potential development of MSC-based therapeutic strategies.<\/jats:p>","DOI":"10.3390\/ijms26052314","type":"journal-article","created":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T05:54:38Z","timestamp":1741154078000},"page":"2314","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Targeting Lung Damage: Amniotic Mesenchymal Stem Cells Mitigate Lipopolysaccharide-Induced Acute Lung Injury via Multiple Signaling Pathways"],"prefix":"10.3390","volume":"26","author":[{"given":"Xinhui","family":"Niu","sequence":"first","affiliation":[{"name":"Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Zhang","sequence":"additional","affiliation":[{"name":"NMPA Key Laboratory for Quality Control of Cell and Gene Therapy Medicine Products, Northeast Normal University, Changchun 130024, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoliang","family":"Xing","sequence":"additional","affiliation":[{"name":"NMPA Key Laboratory for Quality Control of Cell and Gene Therapy Medicine Products, Northeast Normal University, Changchun 130024, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinrui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yating","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4021-1067","authenticated-orcid":false,"given":"Yi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4664-8940","authenticated-orcid":false,"given":"Manman","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2495","DOI":"10.1182\/blood-2018-03-841593","article-title":"Platelet-derived TLT-1 is a prognostic indicator in ALI\/ARDS and prevents tissue damage in the lungs in a mouse model","volume":"132","author":"Deal","year":"2018","journal-title":"Blood"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1038\/s41419-021-04115-7","article-title":"Endotoxin stabilizes protein arginine methyltransferase 4 (PRMT4) protein triggering death of lung epithelia","volume":"12","author":"Lai","year":"2021","journal-title":"Cell Death Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2520348","DOI":"10.1155\/2022\/2520348","article-title":"Melatonin Suppresses Macrophage M1 Polarization and ROS-Mediated Pyroptosis via Activating ApoE\/LDLR Pathway in Influenza A-Induced Acute Lung Injury","volume":"2022","author":"Xu","year":"2022","journal-title":"Oxid. 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