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(ME\/CFS) is a debilitating disorder characterized by serious physical and cognitive impairments. Recent research underscores the role of immune dysfunction, including the role of autoantibodies, in ME\/CFS pathophysiology. Expanding on previous studies, we analyzed 7542 antibody\u2013antigen interactions in ME\/CFS patients using two advanced platforms: a 1134 autoantibody Luminex panel from Oncimmune and Augmenta Bioworks, along with Rapid Extracellular Antigen Profiling (REAP), a validated high-throughput method that measures autoantibody reactivity against 6183 extracellular human proteins and 225 human viral pathogen proteins. Unlike earlier reports, our analysis of 172 participants revealed no significant differences in autoantibody reactivities between ME\/CFS patients and controls, including against GPCRs such as \u03b2-adrenergic receptors. However, subtle trends in autoantibody ratios between male and female ME\/CFS subgroups, along with patterns of herpesvirus reactivation, suggest the need for broader and more detailed exploration.<\/jats:p>","DOI":"10.3390\/ijms26062799","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T05:03:59Z","timestamp":1742447039000},"page":"2799","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["An In-Depth Exploration of the Autoantibody Immune Profile in ME\/CFS Using Novel Antigen Profiling Techniques"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0111-0012","authenticated-orcid":false,"given":"Arnaud","family":"Germain","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jillian R.","family":"Jaycox","sequence":"additional","affiliation":[{"name":"Division of Translational Science and Therapeutics, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA"},{"name":"Department of Immunobiology, Yale School of Medicine, New Haven, CT 06510, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0536-6257","authenticated-orcid":false,"given":"Christopher J.","family":"Emig","sequence":"additional","affiliation":[{"name":"Augmenta Bioworks, Inc., Menlo Park, CA 94025, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron M.","family":"Ring","sequence":"additional","affiliation":[{"name":"Division of Translational Science and Therapeutics, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8141-3058","authenticated-orcid":false,"given":"Maureen R.","family":"Hanson","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,20]]},"reference":[{"key":"ref_1","unstructured":"Institute of Medicine (2015). Beyond Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome: Redefining an Illness, National Academies Press."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Deumer, U.S., Varesi, A., Floris, V., Savioli, G., Mantovani, E., Lopez-Carrasco, P., Rosati, G.M., Prasad, S., and Ricevuti, G. (2021). Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome (Me\/Cfs): An Overview. J. Clin. Med., 10.","DOI":"10.3390\/jcm10204786"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"100720","DOI":"10.1016\/j.bbih.2023.100720","article-title":"Identification of Cd8 T-Cell Dysfunction Associated with Symptoms in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome (Me\/Cfs) and Long COVID and Treatment with a Nebulized Antioxidant\/Anti-Pathogen Agent in a Retrospective Case Series","volume":"36","author":"Gil","year":"2024","journal-title":"Brain Behav. Immun. Health"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1172\/JCI132185","article-title":"Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome Patients Exhibit Altered T Cell Metabolism and Cytokine Associations","volume":"130","author":"Mandarano","year":"2020","journal-title":"J. Clin. Investig."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Maya, J. (2023). Surveying the Metabolic and Dysfunctional Profiles of T Cells and Nk Cells in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241511937"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Milivojevic, M., Che, X., Bateman, L., Cheng, A., Garcia, B.A., Hornig, M., Huber, M., Klimas, N.G., Lee, B., and Lee, H. (2020). Plasma Proteomic Profiling Suggests an Association between Antigen Driven Clonal B Cell Expansion and Me\/Cfs. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0236148"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Rivas, J.L., Palencia, T., Fernandez, G., and Garcia, M. (2018). Association of T and Nk Cell Phenotype with the Diagnosis of Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome (Me\/Cfs). Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.01028"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Seton, K.A., Espejo-Oltra, J.A., Gimenez-Orenga, K., Haagmans, R., Ramadan, D.J., Mehlsen, J., and European ME Research Group for Early Career Researchers (Young EMERG) (2024). Advancing Research and Treatment: An Overview of Clinical Trials in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome (Me\/Cfs) and Future Perspectives. J. Clin. Med., 13.","DOI":"10.3390\/jcm13020325"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"101373","DOI":"10.1016\/j.xcrm.2023.101373","article-title":"Single-Cell Transcriptomics of the Immune System in Me\/Cfs at Baseline and Following Symptom Provocation","volume":"5","author":"Vu","year":"2024","journal-title":"Cell Rep. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/j.autrev.2018.01.009","article-title":"Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome-Evidence for an Autoimmune Disease","volume":"17","author":"Sotzny","year":"2018","journal-title":"Autoimmun. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Fluge, O., Bruland, O., Risa, K., Storstein, A., Kristoffersen, E.K., Sapkota, D., Naess, H., Dahl, O., Nyland, H., and Mella, O. (2011). Benefit from B-Lymphocyte Depletion Using the Anti-Cd20 Antibody Rituximab in Chronic Fatigue Syndrome. A Double-Blind and Placebo-Controlled Study. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0026358"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Fluge, O., Risa, K., Lunde, S., Alme, K., Rekeland, I.G., Sapkota, D., Kristoffersen, E.K., Sorland, K., Bruland, O., and Dahl, O. (2015). B-Lymphocyte Depletion in Myalgic Encephalopathy\/Chronic Fatigue Syndrome. An Open-Label Phase Ii Study with Rituximab Maintenance Treatment. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0129898"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"585","DOI":"10.7326\/M18-1451","article-title":"B-Lymphocyte Depletion in Patients with Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome: A Randomized, Double-Blind, Placebo-Controlled Trial","volume":"170","author":"Fluge","year":"2019","journal-title":"Ann. Intern. Med."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Rekeland, I.G., Sorland, K., Neteland, L.L., Fossa, A., Alme, K., Risa, K., Dahl, O., Tronstad, K.J., Mella, O., and Fluge, O. (2024). Six-Year Follow-up of Participants in Two Clinical Trials of Rituximab or Cyclophosphamide in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0307484"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Scheibenbogen, C., Loebel, M., Freitag, H., Krueger, A., Bauer, S., Antelmann, M., Doehner, W., Scherbakov, N., Heidecke, H., and Reinke, P. (2018). Immunoadsorption to Remove Ss2 Adrenergic Receptor Antibodies in Chronic Fatigue Syndrome Cfs\/Me. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0193672"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Tolle, M., Freitag, H., Antelmann, M., Hartwig, J., Schuchardt, M., van der Giet, M., Eckardt, K.U., Grabowski, P., and Scheibenbogen, C. (2020). Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome: Efficacy of Repeat Immunoadsorption. J. Clin. Med., 9.","DOI":"10.3390\/jcm9082443"},{"key":"ref_17","first-page":"225","article-title":"Autoantibodies against Muscarinic Cholinergic Receptor in Chronic Fatigue Syndrome","volume":"12","author":"Tanaka","year":"2003","journal-title":"Int. J. Mol. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.bbi.2015.09.013","article-title":"Antibodies to Beta Adrenergic and Muscarinic Cholinergic Receptors in Patients with Chronic Fatigue Syndrome","volume":"52","author":"Loebel","year":"2016","journal-title":"Brain Behav. Immun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"100107","DOI":"10.1016\/j.bbih.2020.100107","article-title":"Autoantibodies to Beta-Adrenergic and Muscarinic Cholinergic Receptors in Myalgic Encephalomyelitis (Me) Patients\u2014A Validation Study in Plasma and Cerebrospinal Fluid from Two Swedish Cohorts","volume":"7","author":"Bynke","year":"2020","journal-title":"Brain Behav. Immun. Health"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Ryabkova, V., Gavrilova, N.Y., Poletaeva, A.A., Pukhalenko, A.I., Koshkina, I.A., Churilov, L.P., and Shoenfeld, Y. (2023). Autoantibody Correlation Signatures in Fibromyalgia and Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome: Association with Symptom Severity. Biomedicines, 11.","DOI":"10.3390\/biomedicines11020257"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5224","DOI":"10.1038\/s41467-018-07598-9","article-title":"Gpcr-Specific Autoantibody Signatures Are Associated with Physiological and Pathological Immune Homeostasis","volume":"9","author":"Marques","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1038\/s41467-022-28905-5","article-title":"Autoantibodies Targeting Gpcrs and Ras-Related Molecules Associate with COVID-19 Severity","volume":"13","author":"Halpert","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.it.2008.10.002","article-title":"Naturally Occurring Auto-Antibodies in Homeostasis and Disease","volume":"30","author":"Lutz","year":"2009","journal-title":"Trends Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1038\/nrrheum.2017.134","article-title":"Functional Autoantibodies Targeting G Protein-Coupled Receptors in Rheumatic Diseases","volume":"13","author":"Riemekasten","year":"2017","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Nagele, E.P., Han, M., Acharya, N.K., DeMarshall, C., Kosciuk, M.C., and Nagele, R.G. (2013). Natural Igg Autoantibodies Are Abundant and Ubiquitous in Human Sera, and Their Number Is Influenced by Age, Gender, and Disease. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0060726"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e2115695119","DOI":"10.1073\/pnas.2115695119","article-title":"Autoreactive Antibodies Control Blood Glucose by Regulating Insulin Homeostasis","volume":"119","author":"Amendt","year":"2022","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"100894","DOI":"10.1016\/j.xcrm.2022.100894","article-title":"Inactive Disease in Patients with Lupus Is Linked to Autoantibodies to Type I Interferons That Normalize Blood Ifnalpha and B Cell Subsets","volume":"4","author":"Bradford","year":"2023","journal-title":"Cell Rep. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"103","DOI":"10.7326\/0003-4819-116-2-103","article-title":"A Chronic Illness Characterized by Fatigue, Neurologic and Immunologic Disorders, and Active Human Herpesvirus Type 6 Infection","volume":"116","author":"Buchwald","year":"1992","journal-title":"Ann. Intern. Med."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Lee, J.S., Lacerda, E.M., Nacul, L., Kingdon, C.C., Norris, J., O\u2019Boyle, S., Roberts, C.H., Palla, L., Riley, E.M., and Cliff, J.M. (2021). Salivary DNA Loads for Human Herpesviruses 6 and 7 Are Correlated with Disease Phenotype in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome. Front. Med., 8.","DOI":"10.3389\/fmed.2021.656692"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e2437","DOI":"10.1002\/rmv.2437","article-title":"Reactivation of Herpesviruses During COVID-19: A Systematic Review and Meta-Analysis","volume":"33","author":"Shafiee","year":"2023","journal-title":"Rev. Med. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1300\/J092v11n01_02","article-title":"Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome: Clinical Working Case Definition, Diagnostic and Treatment Protocols","volume":"11","author":"Carruthers","year":"2003","journal-title":"J. Chronic Fatigue Syndr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"48507","DOI":"10.18632\/oncotarget.17397","article-title":"Implementing Liquid Biopsies into Clinical Decision Making for Cancer Immunotherapy","volume":"8","author":"Quandt","year":"2017","journal-title":"Oncotarget"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Welberry, C., Macdonald, I., McElveen, J., Parsy-Kowalska, C., Allen, J., Healey, G., Irving, W., Murray, A., and Chapman, C. (2020). Tumor-Associated Autoantibodies in Combination with Alpha-Fetoprotein for Detection of Early Stage Hepatocellular Carcinoma. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0232247"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1136\/annrheumdis-2018-eular.2383","article-title":"Thu0042 Cancer Immunotherapy Array: A Novel Screening Tool for Immune System Profiling in Cancer Immunotherapy Bridging Autoimmunity and Cancer","volume":"77","author":"Budde","year":"2018","journal-title":"Ann. Rheum. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1186\/s12967-021-03184-8","article-title":"Paradoxical Sex-Specific Patterns of Autoantibody Response to Sars-Cov-2 Infection","volume":"19","author":"Liu","year":"2021","journal-title":"J. Transl. Med."},{"key":"ref_36","unstructured":"The RA-MAP Consortium (2022). Ra-Map, Molecular Immunological Landscapes in Early Rheumatoid Arthritis and Healthy Vaccine Recipients. Sci. Data, 9, 196."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"102583","DOI":"10.1016\/j.autrev.2020.102583","article-title":"Autoantibodies to Stratify Systemic Sclerosis Patients into Clinically Actionable Subsets","volume":"19","author":"Mahler","year":"2020","journal-title":"Autoimmun. Rev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.jim.2018.05.011","article-title":"Label-Free Microarray-Based Detection of Autoantibodies in Human Serum","volume":"459","author":"Bucukovski","year":"2018","journal-title":"J. Immunol. Methods"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"O\u2019Neal, A.J., Glass, K.A., Emig, C.J., Vitug, A.A., Henry, S.J., Shungu, D.C., Mao, X., Levine, S.M., and Hanson, M.R. (2022). Survey of Anti-Pathogen Antibody Levels in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome. Proteomes, 10.","DOI":"10.3390\/proteomes10020021"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1073\/pnas.0905778107","article-title":"G Protein-Coupled Receptor Kinase 2 (Grk2) Modulation and Cell Cycle Progression","volume":"107","author":"Penela","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/s00281-018-0723-8","article-title":"Sex Differences in Autoimmune Disorders of the Central Nervous System","volume":"41","author":"Gold","year":"2019","journal-title":"Semin. Immunopathol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Fagan, T.F., and Faustman, D.L. (2004). Sex Differences in Autoimmunity. Advances in Molecular and Cell Biology, Elsevier.","DOI":"10.1016\/S1569-2558(03)34020-2"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Edwards, M., Dai, R., and Ahmed, S.A. (2018). Our Environment Shapes Us: The Importance of Environment and Sex Differences in Regulation of Autoantibody Production. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.00478"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.curtheres.2014.06.004","article-title":"The Prevalence of Antinuclear Antibodies in the General Population of China: A Cross-Sectional Study","volume":"76","author":"Guo","year":"2014","journal-title":"Curr. Ther. Res. Clin. Exp."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1038\/nri.2016.90","article-title":"Sex Differences in Immune Responses","volume":"16","author":"Klein","year":"2016","journal-title":"Nat. Rev. Immunol."},{"key":"ref_46","first-page":"42","article-title":"Serum Levels of Immunoglobulins (Igg, Iga, Igm) in a General Adult Population and Their Relationship with Alcohol Consumption, Smoking and Common Metabolic Abnormalities","volume":"151","author":"Alende","year":"2008","journal-title":"Clin. Exp. Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Freitag, H., Szklarski, M., Lorenz, S., Sotzny, F., Bauer, S., Philippe, A., Kedor, C., Grabowski, P., Lange, T., and Riemekasten, G. (2021). Autoantibodies to Vasoregulative G-Protein-Coupled Receptors Correlate with Symptom Severity, Autonomic Dysfunction and Disability in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome. J. Clin. Med., 10.","DOI":"10.3390\/jcm10163675"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Sotzny, F., Filgueiras, I.S., Kedor, C., Freitag, H., Wittke, K., Bauer, S., Sepulveda, N., da Fonseca, D.L.M., Baiocchi, G.C., and Marques, A.H.C. (2022). Dysregulated Autoantibodies Targeting Vaso- and Immunoregulatory Receptors in Post Covid Syndrome Correlate with Symptom Severity. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.981532"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1159\/000517930","article-title":"Human Herpesvirus 6 Infection and Risk of Chronic Fatigue Syndrome: A Systematic Review and Meta-Analysis","volume":"65","author":"Mozhgani","year":"2022","journal-title":"Intervirology"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Ruiz-Pablos, M., Paiva, B., Montero-Mateo, R., Garcia, N., and Zabaleta, A. (2021). Epstein-Barr Virus and the Origin of Myalgic Encephalomyelitis or Chronic Fatigue Syndrome. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.656797"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1038\/s41586-023-06651-y","article-title":"Distinguishing Features of Long Covid Identified through Immune Profiling","volume":"623","author":"Klein","year":"2023","journal-title":"Nature"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the False Discovery Rate: A Practical and Powerful Approach to Multiple Testing","volume":"57","author":"Benjamini","year":"1995","journal-title":"J. R. Stat. Soc. Ser. B"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1038\/s41586-021-03631-y","article-title":"Diverse Functional Autoantibodies in Patients with COVID-19","volume":"595","author":"Wang","year":"2021","journal-title":"Nature"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"100172","DOI":"10.1016\/j.crmeth.2022.100172","article-title":"High-Throughput Identification of Autoantibodies That Target the Human Exoproteome","volume":"2","author":"Wang","year":"2022","journal-title":"Cell Rep. 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