{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:13:56Z","timestamp":1783815236742,"version":"3.55.0"},"reference-count":34,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Mahasarakham University","award":["6517043\/2565"],"award-info":[{"award-number":["6517043\/2565"]}]},{"name":"Mahasarakham University","award":["TUFF53\/2566"],"award-info":[{"award-number":["TUFF53\/2566"]}]},{"name":"Thailand Science Research and Innovation Fundamental Fund","award":["6517043\/2565"],"award-info":[{"award-number":["6517043\/2565"]}]},{"name":"Thailand Science Research and Innovation Fundamental Fund","award":["TUFF53\/2566"],"award-info":[{"award-number":["TUFF53\/2566"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study investigates the prevalence of parasitic helminths in free-ranging domestic cats and dogs near the Chi River and natural reservoirs in Maha Sarakham Province, Thailand. Fecal samples from 39 cats and 148 dogs were analyzed using a modified formalin-ether concentration technique (FECT). The overall prevalence of helminth infections was 64.1% in cats and 51.4% in dogs. Common parasites were detected including soil-transmitted species like Ancylostoma sp. (hookworm), Toxocara spp., and Strongyloides sp., as well as foodborne helminths such as Taenia sp., Hymenolepis sp., Spirometra sp., and Opisthorchis sp. Multiple parasitic infections were commonly found in dogs (57.9%) and cats (46.2%). Our findings suggest that domestic cats and dogs act as important reservoirs for zoonotic helminths in the region. Notably, Opisthorchis viverrini-like eggs were found exclusively in cats, with a prevalence of 23.1%. The intron 5 of domain 1 of the taurocyamine kinase gene (TkD1Int5) was used for genotyping O. viverrini-like eggs. All O. viverrini-like egg samples with TkD1Int5 haplotypes (Ov116\u2013Ov123) were uniquely found in cats. Genetic analysis revealed that TkD1Int5 haplotypes were similar to those previously reported for Opisthorchis viverrini in various species of cyprinid fish across opisthorchiasis-endemic regions in Thailand and Lao PDR. Three TkD1Int5 haplogroups (I\u2013III) were classified, with O. viverrini-like eggs from cats distributed across all haplogroups. Notably, one haplotype (Ov118) was genetically distinct from the others and did not cluster into any haplogroup. These findings highlight the crucial role of cats as reservoir hosts and their potential contribution to the transmission of the zoonotic liver fluke O.\u00a0viverrini, posing a notable public health concern.<\/jats:p>","DOI":"10.3390\/ijms26073005","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T04:17:14Z","timestamp":1742962634000},"page":"3005","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Parasitic Helminth Infections and Intron Sequence Genotyping of Opisthorchis viverrini-like Eggs in Outdoor Domestic Cats and Dogs Across the Chi River Basin, Maha Sarakham Province, Thailand"],"prefix":"10.3390","volume":"26","author":[{"given":"Kotchaphon","family":"Vaisusuk","sequence":"first","affiliation":[{"name":"Department of Veterinary Technology and Veterinary Nursing, Faculty of Agricultural Technology, Rajabhat Maha Sarakham University, Maha Sarakham 44000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wasupon","family":"Chatan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinic, Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham 44000, Thailand"},{"name":"Center of Excellence in Biodiversity Research, Mahasarakham University, Maha Sarakham 44150, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warayutt","family":"Pilap","sequence":"additional","affiliation":[{"name":"Center of Excellence in Biodiversity Research, Mahasarakham University, Maha Sarakham 44150, Thailand"},{"name":"Walai Rukhavej Botanical Research Insitute, Mahasarakham University, Maha Sarakham 44150, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongjit","family":"Thanchomnang","sequence":"additional","affiliation":[{"name":"Biomedical Science Research Unit, Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2467-8566","authenticated-orcid":false,"given":"Chavanut","family":"Jaroenchaiwattanachote","sequence":"additional","affiliation":[{"name":"Center of Excellence in Biodiversity Research, Mahasarakham University, Maha Sarakham 44150, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9480-1778","authenticated-orcid":false,"given":"Paiboon","family":"Sithithaworn","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ross H.","family":"Andrews","sequence":"additional","affiliation":[{"name":"Department of Surgery & Cancer, Faculty of Medicine, Imperial College, London SW7 2AZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chairat","family":"Tantrawatpan","sequence":"additional","affiliation":[{"name":"Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Center of Excellence in Stem Cell Research and Innovation, Thammasat University, Rangsit Campus, Pathum Thani 12120, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4789-297X","authenticated-orcid":false,"given":"Weerachai","family":"Saijuntha","sequence":"additional","affiliation":[{"name":"Center of Excellence in Biodiversity Research, Mahasarakham University, Maha Sarakham 44150, Thailand"},{"name":"Biomedical Science Research Unit, Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/s13071-023-05670-y","article-title":"Zoonotic parasites associated with predation by dogs and cats","volume":"16","author":"Roldan","year":"2023","journal-title":"Parasit. Vectors"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/bs.apar.2018.05.003","article-title":"Reservoir animals and their roles in transmission of Opisthorchis viverrini","volume":"101","author":"Tangkawattana","year":"2018","journal-title":"Adv. Parasitol."},{"key":"ref_3","first-page":"1209","article-title":"A surveillance of canine gastrointestinal parasites in fecal samples from public areas of Bangkok, Thailand","volume":"5","author":"Kaewthamasorn","year":"2006","journal-title":"J. Animal Vet. Adv."},{"key":"ref_4","first-page":"69","article-title":"Gastrointestinal parasites of stray cats in Bangkok Metropolitan Areas, Thailand","volume":"41","author":"Jittapalapong","year":"2007","journal-title":"Kasetsart J. (Nat. Sci.)"},{"key":"ref_5","first-page":"31","article-title":"Gastrointestinal parasites of dogs and cats in a refuge in Nakhon Nayok, Thailand","volume":"45","author":"Rojekittikhun","year":"2014","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1292\/jvms.16-0293","article-title":"Prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower Northern Thailand","volume":"78","author":"Pumidonming","year":"2016","journal-title":"J. Vet. Med. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.parint.2011.08.004","article-title":"High prevalence of Opisthorchis viverrini infection in reservoir hosts in four districts of Khon Kaen Province, an opisthorchiasis endemic area of Thailand","volume":"61","author":"Aunpromma","year":"2012","journal-title":"Parasitol. Int."},{"key":"ref_8","first-page":"1153","article-title":"Prevalence and risk factors forOpisthorchis viverrini infection among cats and dogs in six districts surrounding the Ubonratana Dam, an endemic area for human opisthorchiasis in Northeastern Thailand","volume":"47","author":"Aunpromma","year":"2016","journal-title":"Southeast Asian J. Trop. Med. Publ. Health"},{"key":"ref_9","first-page":"2853","article-title":"A large scale study of the epidemiology and risk factors for the carcinogenic liver fluke Opisthorchis viverrini in Udon Thani Province, Thailand","volume":"18","author":"Prakobwong","year":"2017","journal-title":"Asian Pac. J. Cancer Prev."},{"key":"ref_10","first-page":"10","article-title":"Comprehensive Flood Mitigation and Management in the Chi River Basin, Thailand","volume":"13","author":"Kuntiyawichai","year":"2011","journal-title":"Lowland Technol. Int."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/978-3-030-18616-6_6","article-title":"Liver flukes: Clonorchis and Opisthorchis","volume":"Volume 1154","author":"Toledo","year":"2019","journal-title":"Digenetic Trematodes"},{"key":"ref_12","first-page":"36","article-title":"Prevalence of Opisthorchis viverrini infection in the canine and feline hosts in three villages, Khon Kane Province, northeast Thailand","volume":"41","author":"Enes","year":"2010","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1645\/GE-2764.1","article-title":"Discovery of Opisthorchis lobatus (Trematoda: Opisthorchiidae): A new record of small liver flukes in the Greater Mekong Sub-region","volume":"97","author":"Thaenkham","year":"2011","journal-title":"J. Parasitol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1093\/trstmh\/trv103","article-title":"Clonorchis sinensis and Opisthorchis spp. in Vietnam: Current status and prospects","volume":"110","author":"Doanh","year":"2016","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1186\/s13071-017-2514-9","article-title":"Updated molecular phylogenetic data for Opisthorchis spp. (Trematoda: Opisthorchioidea) from ducks in Vietnam","volume":"10","author":"Dao","year":"2017","journal-title":"Parasit. Vec."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"357","DOI":"10.3347\/kjp.2016.54.3.357","article-title":"Prevalence of Opisthorchis viverrini-like fluke infection in ducks in Binh Dinh Province, central Vietnam","volume":"54","author":"Dao","year":"2016","journal-title":"Korean J. Parasitol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1017\/S0031182022000646","article-title":"Does Opisthorchis viverrini circulate between humans and domestic cats in an endemic area in Thailand?","volume":"149","author":"Sato","year":"2022","journal-title":"Parasitology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.ijpara.2024.04.006","article-title":"Exploring the Second Intermediate Hosts and Morphology of Human- and Cat-Specific Opisthorchis viverrini-Like Populations","volume":"54","author":"Agustina","year":"2024","journal-title":"Int. J. Parasitol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2485","DOI":"10.1007\/s00436-020-06734-z","article-title":"Intron sequence variation of the echinostomes (Trematoda; Echinostomatidae): Implications for genetic investigations of the 37 collar-spined, Echinostoma miyagawai Ischii, 1932 and E. revolutum (Fr\u00f6elich, 1802)","volume":"199","author":"Saijuntha","year":"2020","journal-title":"Parasitol. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"104761","DOI":"10.1016\/j.meegid.2021.104761","article-title":"Genetic differentiation of Southeast Asian Paragonimus Braun 1899 (Digenea: Paragonimidae) and genetic variation in the Paragonimus heterotremus complex examined by nuclear DNA sequences","volume":"90","author":"Tantrawatpan","year":"2021","journal-title":"Infect. Genet. Evol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Tantrawatpan, C., Maleewong, W., Thanchomnang, T., Pilap, W., Agatsuma, T., Andrews, R.H., Sithithaworn, P., and Saijuntha, W. (2023). Characterization of intron regions and its potential as genetic marker for population genetic investigation of zoonotic liver fluke Opisthorchis viverrini sensu lato and Clonorchis sinensis from various geographical isolates and animal hosts. Animals, 13.","DOI":"10.3390\/ani13203200"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Zanzani, S.A., Gazzonis, A.L., Scarpa, P., Berrilli, F., and Manfredi, M.T. (2014). Intestinal parasites of owned dogs and cats from metropolitan and micropolitan areas: Prevalence, zoonotic risks, and pet owner awareness in northern Italy. Biomed. Res. Int., 2014.","DOI":"10.1155\/2014\/696508"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1111\/j.1863-2378.2008.01163.x","article-title":"Prevalence of dog intestinal parasites and risk perception of zoonotic infection by dog owners in S\u00e3o Paulo State, Brazil","volume":"55","author":"Katagiri","year":"2008","journal-title":"Zoonoses Public Health"},{"key":"ref_24","first-page":"247","article-title":"Intestinal helminth frequency in stray dogs sacrificed in the City of Queretaro, in the State of Queretaro, in Mexico","volume":"33","year":"2002","journal-title":"Vet. M\u00e9xico"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1590\/S1984-29612016019","article-title":"Gastrointestinal parasites of cats in Brazil: Frequency and zoonotic risk","volume":"25","author":"Monteiro","year":"2016","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e110","DOI":"10.1017\/S0022149X1900107X","article-title":"Dogs are reservoir hosts for possible transmission of human strongyloidiasis in Thailand: Molecular identification and genetic diversity of causative parasite species","volume":"94","author":"Sanpool","year":"2020","journal-title":"J. Helminthol."},{"key":"ref_27","first-page":"17","article-title":"Prevalence of helminth parasites of dogs and owners awareness about zoonotic parasites in Ambo town, central Ethiopia","volume":"14","author":"Zewdu","year":"2010","journal-title":"Ethiop. Vet. J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1038\/ncomms2380","article-title":"The impact of free-ranging domestic cats on wildlife of the United States","volume":"4","author":"Loss","year":"2013","journal-title":"Nat. Commun."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1071\/AM03051","article-title":"Home Range of House Cats Felis catus Living within a National Park","volume":"25","author":"Meek","year":"2003","journal-title":"Aust. Mammal."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/0035-9203(90)90159-C","article-title":"A high frequency of hepatobiliary disease and suspected cholangiocarcinoma associated with heavy Opisthorchis viverrini infection in a small community in northeast Thailand","volume":"84","author":"Elkins","year":"1990","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_31","unstructured":"Zajac, A.M., and Conboy, G.A. (2012). Textbook of Veterinary Clinical Parasitology, Wiley-Blackwell."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"Clustal W and Clustal X version 2.0","volume":"23","author":"Larkin","year":"2007","journal-title":"Bioinformatics"},{"key":"ref_33","first-page":"95","article-title":"BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp. Ser."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1093\/oxfordjournals.molbev.a026036","article-title":"Median-joining networks for inferring intraspecific phylogenies","volume":"16","author":"Bandelt","year":"1999","journal-title":"Mol. Biol. Evol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/7\/3005\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:00:15Z","timestamp":1760029215000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/7\/3005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,26]]},"references-count":34,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["ijms26073005"],"URL":"https:\/\/doi.org\/10.3390\/ijms26073005","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,26]]}}}