{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:05:49Z","timestamp":1760058349304,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ohio State University Comprehensive Cancer Center and the NIH","award":["P30 CA016058","R01 GM108743"],"award-info":[{"award-number":["P30 CA016058","R01 GM108743"]}]},{"name":"Ohio State University Comprehensive Cancer Center\/Department of Radiation Oncology","award":["P30 CA016058","R01 GM108743"],"award-info":[{"award-number":["P30 CA016058","R01 GM108743"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The role of p31Comet in deactivating the spindle assembly checkpoint is well described in the literature; however, the data are all completed using Variant 2 of p31Comet. p31Comet is known to be expressed as two different splice variants: Variant 1 and Variant 2. Variant 1 contains an additional 32 N-terminal residues compared to Variant 2. We report that Variant 1 exhibits a reduced ability to bind to MAD2 and thus a reduced ability to induce mitotic progression. Additionally, we show that Variant 1 exhibits reduced stability compared to Variant 2. We further show that Variant 1 is uniquely expressed in the Testes, indicating a potentially unique role of Variant 1 in that organ. Overall, we demonstrate the N-terminus of p31Comet is capable of modulating p31Comet activity in mitosis.<\/jats:p>","DOI":"10.3390\/ijms26073089","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T04:11:40Z","timestamp":1743135100000},"page":"3089","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["p31Comet Splice Variants Induce Distinct Spindle Assembly Checkpoint Dynamics due to Their Unique N-Termini"],"prefix":"10.3390","volume":"26","author":[{"given":"Luke","family":"Scarberry","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center, College of Medicine, The Ohio State University, Columbus, OH 43210, USA"},{"name":"Biomedical Sciences Graduate Program, College of Medicine, The Ohio State University, Columbus, OH 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5387-5909","authenticated-orcid":false,"given":"Garrett","family":"Thesing","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center, College of Medicine, The Ohio State University, Columbus, OH 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Brennan","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center, College of Medicine, The Ohio State University, Columbus, OH 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madison","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center, College of Medicine, The Ohio State University, Columbus, OH 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2482-0332","authenticated-orcid":false,"given":"Matthew K.","family":"Summers","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center, College of Medicine, The Ohio State University, Columbus, OH 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1038\/35053094","article-title":"MAD2 haplo-insufficiency causes premature anaphase and chromosome instability in mammalian cells","volume":"409","author":"Michel","year":"2001","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e170079","DOI":"10.1172\/jci.insight.170079","article-title":"Biallelic MAD2L1BP (p31comet) mutation is associated with mosaic aneuploidy and juvenile granulosa cell tumors","volume":"8","author":"Hellmuth","year":"2023","journal-title":"JCI Insight"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/S0092-8674(00)80875-2","article-title":"Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2","volume":"101","author":"Dobles","year":"2000","journal-title":"Cell"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.ccr.2009.10.023","article-title":"Whole chromosome instability caused by Bub1 insufficiency drives tumorigenesis through tumor suppressor gene loss of heterozygosity","volume":"16","author":"Baker","year":"2009","journal-title":"Cancer Cell"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e85649","DOI":"10.7554\/eLife.85649","article-title":"Biallelic variants in MAD2L1BP (p31(comet)) cause female infertility characterized by oocyte maturation arrest","volume":"12","author":"Huang","year":"2023","journal-title":"Elife"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1002\/ijc.24094","article-title":"Spindle assembly checkpoint and p53 deficiencies cooperate for tumorigenesis in mice","volume":"124","author":"Chi","year":"2009","journal-title":"Int. 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