{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:49:18Z","timestamp":1787028558867,"version":"build-2736575974"},"reference-count":48,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T00:00:00Z","timestamp":1743206400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Klebsiella oxytoca bacilli co-form the human intestinal microbiota, but in favorable conditions, they may also affect immunocompromised individuals, causing urinary tract infections, bacteremia, or antibiotic-associated hemorrhagic colitis. The growing numbers of clinical outbreaks of K. oxytoca infections make these bacteria an emerging pathogen, which is still masked by the predominant K. pneumoniae isolates. Thus, it is very important to advance knowledge on K. oxytoca pathogenicity. This work aims to characterize a urine isolate, K. oxytoca 0.062, from central Poland, which appears to present a multidrug-resistant and extended-spectrum \u03b2-lactamases-positive phenotype. The structural experiments include sugar and methylation analyses, mass spectrometry, and 1H and 13C Nuclear Magnetic Resonance (NMR) spectroscopy. Additionally, 1H,1H ROESY, and 1H,13C HMBC experiments were carried out on the high-molecular-weight O polysaccharide fraction of K. oxytoca lipopolysaccharides (LPSs). These analyses led to the detection of two polysaccharide antigens: one neutral, containing a linear trisaccharide unit called mannan, and one acidic, which is built up of a branched tetrasaccharide unit containing two mannopyranose (\u03b1-Manp) residues, one galactopyranose (\u03b2-Galp) residue, and one galacturonic acid (\u03b1-GalpA) residue. The GalpA residue seems to be a potential minor epitope, recognized by the selected Proteus antisera in the serological studies.<\/jats:p>","DOI":"10.3390\/ijms26073177","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T03:25:02Z","timestamp":1743391502000},"page":"3177","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The First Report on the Structure of Polysaccharide Surface Antigens of the Clinical Klebsiella oxytoca 0.062 Strain and the Contribution in the Serological Cross-Reactions"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7425-3719","authenticated-orcid":false,"given":"Agata","family":"Palusiak","sequence":"first","affiliation":[{"name":"Department of Biology of Bacteria, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12\/16, 90-237 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2975-9593","authenticated-orcid":false,"given":"Anna","family":"Turska-Szewczuk","sequence":"additional","affiliation":[{"name":"Department of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e00006-21","DOI":"10.1128\/CMR.00006-21","article-title":"Klebsiella oxytoca complex: Update on taxonomy, antimicrobial resistance, and virulence","volume":"35","author":"Yang","year":"2022","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","first-page":"000592","article-title":"Improved molecular characterization of the Klebsiella oxytoca complex reveals the prevalence of the kleboxymycin biosynthetic gene cluster","volume":"7","author":"Shibu","year":"2021","journal-title":"Microb. Genom."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Stewart, J., Judd, L.M., Jenney, A., Holt, K.E., Wyres, K.L., and Hawkey, J. (2022). Epidemiology and genomic analysis of Klebsiella oxytoca from a single hospital network in Australia. BMC Infect. Dis., 22.","DOI":"10.1186\/s12879-022-07687-7"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1093\/gbe\/evx019","article-title":"Population structure of multidrug-resistant Klebsiella oxytoca within hospitals across the United Kingdom and Ireland identifies sharing of virulence and resistance genes with K. pneumoniae","volume":"9","author":"Moradigaravand","year":"2017","journal-title":"Genome Biol. Evol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Palusiak, A. (2022). Proteus mirabilis and Klebsiella pneumoniae as pathogens capable of causing co-infections and exhibiting similarities in their virulence factors. Front. Cell. Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.991657"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4198","DOI":"10.1128\/JB.187.12.4198-4206.2005","article-title":"A second outer-core region in Klebsiella pneumoniae lipopolysaccharide","volume":"187","author":"Izquierdo","year":"2005","journal-title":"J. Bacteriol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Knirel, Y.A., and Valvano, M.A. (2011). Structure of O-antigens. Bacterial Lipopolysaccharides, Springer.","DOI":"10.1007\/978-3-7091-0733-1"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1177\/1753425909360668","article-title":"Structure and serology of O-antigens as the basis for classification of Proteus strains","volume":"17","author":"Knirel","year":"2011","journal-title":"Innate. Immun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.molimm.2014.11.016","article-title":"The antigens contributing to the serological cross-reactions of Proteus antisera with Klebsiella representatives","volume":"64","author":"Palusiak","year":"2015","journal-title":"Mol. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Palusiak, A. (2021). The contribution of polysaccharide antigens from clinical Proteus spp. and Klebsiella spp. isolates to the serological cross-reactions. Front. Cell. Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.707578"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"121581","DOI":"10.1016\/j.carbpol.2023.121581","article-title":"The OL101 O antigen locus specifies a novel Klebsiella pneumoniae serotype O13 structure","volume":"326","author":"Artyszuk","year":"2024","journal-title":"Carbohydr. Polym."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Drzewiecka, D., Levina, E.A., Shashkov, A.S., Kalinchuk, N.A., and Knirel, Y.A. (2024). Structural and serological characterization of yet another new O antigen, O86, in Proteus mirabilis clinical strains. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252413642"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1016\/j.chom.2021.09.003","article-title":"Klebsiella oxytoca causes colonization resistance against multidrug-resistant K. pneumoniae in the gut via cooperative carbohydrate competition","volume":"29","author":"Osbelt","year":"2021","journal-title":"Cell Host Microbe"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/0008-6215(88)80156-3","article-title":"A computer-assisted structural analysis of regular polysaccharides on the basis of 13C-N.M.R. data","volume":"175","author":"Lipkind","year":"1988","journal-title":"Carbohydr. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1002\/mrc.1260260904","article-title":"Stereochemical factors determining the effects of glycosylation on the 13C chemical shifts in carbohydrates","volume":"26","author":"Shashkov","year":"1988","journal-title":"Magn. Reson. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.1016\/j.carres.2006.08.008","article-title":"Structural elucidation of the O-antigenic polysaccharide from enterohemorrhagic (EHEC) Escherichia coli O48:H21","volume":"341","author":"MacLean","year":"2006","journal-title":"Carbohydr. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0065-2318(08)60055-4","article-title":"Carbon-13 nuclear magnetic resonance spectroscopy of monosaccharides","volume":"41","author":"Bock","year":"1983","journal-title":"Adv. Carbohydr. Chem. Biochem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0008-6215(89)84069-8","article-title":"Computer-assisted structural analysis of polysaccharides with an extended version of casper using 1H- and 13C-n.m.r. data","volume":"188","author":"Jansson","year":"1989","journal-title":"Carbohydr. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"108060","DOI":"10.1016\/j.carres.2020.108060","article-title":"Structure of the O-specific polysaccharide from Azospirillum formosense CC-Nfb-7(T)","volume":"494","author":"Sigida","year":"2020","journal-title":"Carbohydr. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/0008-6215(88)84018-7","article-title":"The Nuclear Overhauser effect and structural factors de-termining the conformations of disaccharide glycosides","volume":"181","author":"Lipkind","year":"1988","journal-title":"Carbohydr. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"25070","DOI":"10.1074\/jbc.M202683200","article-title":"Structures of lipopolysaccharides from Klebsiella pneumoniae. Elucidation of the structure of the linkage region between core and polysaccharide O chain and identification of the residues at the non-reducing termini of the O chains","volume":"277","author":"Vinogradov","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_22","unstructured":"EUCAST (2013). EUCAST Guidelines for Detection of Resistance Mechanisms and Specific Resistances of Clinical and\/or Epidemiological Importance, EUCAST. Available online: https:\/\/www.eucast.org\/resistance_mechanisms."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"768","DOI":"10.3349\/ymj.2010.51.5.768","article-title":"Molecular Characteristics of Extended Spectrum \u03b2-Lactamases in Escherichia coli and Klebsiella pneumoniae and the prevalence of qnr in Extended Spectrum \u03b2-Lactamase isolates in a Tertiary Care Hospital in Korea","volume":"51","author":"Kim","year":"2010","journal-title":"Yonsei Med. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1159\/000500311","article-title":"Phenotypic and genotypic characterization of ESBL-, AmpC-, and carbapenemase-producing Klebsiella pneumoniae and Escherichia coli isolates","volume":"28","author":"Kazemian","year":"2019","journal-title":"Med. Princ. Pract."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Khalifa, S.M., Abd El-Aziz, A.M., Hassan, R., and Abdelmegeed, E.S. (2021). \u03b2-lactam resistance associated with \u03b2-lactamase production and porin alteration in clinical isolates of E. coli and K. pneumoniae. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0251594"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s13756-023-01263-7","article-title":"Carriage of ESBL-producing Klebsiella pneumoniae and Escherichia coli among children in rural Ghana: A cross-sectional study","volume":"12","author":"Akenten","year":"2023","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1093\/jac\/dkl505","article-title":"A novel reverse-line hybridization assay for identifying genotypes of CTX-M-type extended-spectrum \u03b2-lactamases","volume":"59","author":"Ensor","year":"2007","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e01023-21","DOI":"10.1128\/Spectrum.01023-21","article-title":"Mechanistic insights into the capsule-targeting depolymerase from a Klebsiella pneumoniae bacteriophage","volume":"9","author":"Dunstan","year":"2021","journal-title":"Microbiol. Spectr."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Zhu, J., Wang, T., Chen, L., and Du, H. (2021). Virulence factors in hypervirulent Klebsiella pneumoniae. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.642484"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Kaszowska, M., Majkowska-Skrobek, G., Markwitz, P., Lood, C., Jachymek, W., Maciejewska, A., Lukasiewicz, J., and Drulis-Kawa, Z. (2021). The mutation in wbaP cps gene cluster selected by phage-borne depolymerase abolishes capsule production and diminishes the virulence of Klebsiella pneumoniae. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222111562"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1080\/08927014.2016.1231300","article-title":"Role of interspecies interactions in dual-species biofilms developed In vitro by uropathogens isolated from polymicrobial urinary catheter-associated bacteriuria","volume":"32","author":"Mateyca","year":"2016","journal-title":"Biofouling"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1099\/jmm.0.47395-0","article-title":"Species interactions in mixedcommunity crystalline biofilms on urinary catheters","volume":"56","author":"Macleod","year":"2007","journal-title":"J. Med. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"22993","DOI":"10.1074\/jbc.274.33.22993","article-title":"The structure of the colony migration factor from pathogenic Proteus mirabilis. A capsular polysaccharide that facilitates swarming","volume":"274","author":"Rahman","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Kelly, S.D., Ovchinnikova, O.G., M\u00fcller, F., Steffen, M., Braun, M., Sweeney, R.P., Kowarik, M., Follador, R., Lowary, T.L., and Serventi, F. (2023). Identification of a second glycoform of the clinically prevalent O1 antigen from Klebsiella pneumoniae. Microbiology, 120.","DOI":"10.1073\/pnas.2301302120"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0008-6215(88)80157-5","article-title":"The use of bacteriophage-mediated depolymerisation in the structural investigation of the capsular polysaccharide from Escherichia coli serotype K36","volume":"175","author":"Parolis","year":"1988","journal-title":"Carbohydr. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"129","DOI":"10.18388\/abp.2014_1934","article-title":"The amide of galacturonic acid with lysine as an immunodominant component of the lipopolysaccharide core region from Proteus penneri 42 strain","volume":"61","author":"Palusiak","year":"2014","journal-title":"Acta Biochim. Pol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"107831","DOI":"10.1016\/j.carres.2019.107831","article-title":"Structural and serological characterization of the O82 antigen of a Proteus mirabilis strain isolated from a patient in Poland","volume":"486","author":"Levina","year":"2019","journal-title":"Carbohydr. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1099\/mic.0.28611-0","article-title":"The ionic interaction of Klebsiella pneumoniae K2 capsule and core lipopolysaccharide","volume":"152","author":"Fresno","year":"2006","journal-title":"Microbiology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.amjmed.2021.03.047","article-title":"\u2018String Test\u2019 for hypermucoviscous Klebsiella pneumoniae","volume":"134","author":"Eisenmenger","year":"2021","journal-title":"Am. J. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"13","DOI":"10.3109\/10520294109106189","article-title":"Staining bacteria and yeasts with acid dyes","volume":"16","author":"Maneval","year":"1941","journal-title":"Stain. Technol."},{"key":"ref_41","unstructured":"The European Committee on Antimicrobial Susceptibility Testing (2024). Breakpoint Tables for Interpretation of MICs and Zone Diameters, The European Committee on Antimicrobial Susceptibility Testing. Available online: http:\/\/www.eucast.org."},{"key":"ref_42","unstructured":"M100 Clinical and Laboratory Standard Institute (2020). Performance Standards for Antimicrobial Susceptibility Testing, M100 Clinical and Laboratory Standard Institute. [30th ed.]. Available online: https:\/\/www.nih.org.pk."},{"key":"ref_43","first-page":"83","article-title":"Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure","volume":"5","author":"Westphal","year":"1965","journal-title":"Methods Carbohydr. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"108896","DOI":"10.1016\/j.carres.2023.108896","article-title":"Immunochemical studies and gene cluster relationships of closely related O-antigens of Aeromonas hydrophila Pt679, Aeromonas popoffii A4, and Aeromonas sobria K928 strains classified into the PGO1 serogroup dominant in Polish aquaculture of carp and rainbow trout","volume":"531","author":"Kurzylewska","year":"2023","journal-title":"Carbohydr. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0008-6215(84)85242-8","article-title":"A simple and rapid method for the permethylation of carbohydrates","volume":"131","author":"Ciucanu","year":"1984","journal-title":"Carbohydr. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S0008-6215(00)80882-4","article-title":"Assignment of absolute configuration of sugars by g.l.c. of their acetylated glycosides formed from chiral alcohols","volume":"62","author":"Leontein","year":"1978","journal-title":"Carbohydr. Res"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0003-2697(82)90673-X","article-title":"A Sensitive silver-stain for detecting lipopolysaccharide in polyacrylamide gels","volume":"119","author":"Tsai","year":"1982","journal-title":"Anal. Biochem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1006\/abio.1995.1238","article-title":"Improved method for silver staining of glycoproteins in thin sodium dodecyl sulfate polyacrylamide gels","volume":"226","author":"Poulsen","year":"1995","journal-title":"Anal. Biochem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/7\/3177\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:05:16Z","timestamp":1760029516000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/7\/3177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,29]]},"references-count":48,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["ijms26073177"],"URL":"https:\/\/doi.org\/10.3390\/ijms26073177","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,29]]}}}