{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:01:05Z","timestamp":1790190065484,"version":"4.1.0"},"reference-count":64,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T00:00:00Z","timestamp":1743379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Minjiang University Introduces Talent pre-research project","award":["MJY22008"],"award-info":[{"award-number":["MJY22008"]}]},{"name":"Minjiang University Introduces Talent pre-research project","award":["MJY24020"],"award-info":[{"award-number":["MJY24020"]}]},{"name":"Minjiang University Introduces Talent pre-research project","award":["2025F15"],"award-info":[{"award-number":["2025F15"]}]},{"name":"Minjiang University Introduces Talent pre-research project","award":["2021FJSCZY02"],"award-info":[{"award-number":["2021FJSCZY02"]}]},{"name":"Science and Technology Project of Fuzhou institute of Oceanography","award":["MJY22008"],"award-info":[{"award-number":["MJY22008"]}]},{"name":"Science and Technology Project of Fuzhou institute of Oceanography","award":["MJY24020"],"award-info":[{"award-number":["MJY24020"]}]},{"name":"Science and Technology Project of Fuzhou institute of Oceanography","award":["2025F15"],"award-info":[{"award-number":["2025F15"]}]},{"name":"Science and Technology Project of Fuzhou institute of Oceanography","award":["2021FJSCZY02"],"award-info":[{"award-number":["2021FJSCZY02"]}]},{"name":"Seed Industry Innovation and Industrialization in Fujian Province","award":["MJY22008"],"award-info":[{"award-number":["MJY22008"]}]},{"name":"Seed Industry Innovation and Industrialization in Fujian Province","award":["MJY24020"],"award-info":[{"award-number":["MJY24020"]}]},{"name":"Seed Industry Innovation and Industrialization in Fujian Province","award":["2025F15"],"award-info":[{"award-number":["2025F15"]}]},{"name":"Seed Industry Innovation and Industrialization in Fujian Province","award":["2021FJSCZY02"],"award-info":[{"award-number":["2021FJSCZY02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Reproduction and breeding are crucial to maintaining abalone aquaculture. Understanding the molecular underpinnings of sexual maturation is essential for advancing knowledge in reproductive biology. However, the molecular mechanisms of gonadal development in abalones remain poorly understood, particularly in microRNA (miRNA)-mediated regulation. Thus, this study conducted a comprehensive transcriptomic analysis of abalone Haliotis discus hannai (H. discus hannai) to identify genes and miRNAs associated with ovarian and testicular discovery. This study identified 685 differentially expressed (DE) genes between the H. discus hannai ovary (DD_ovary) and testis (DD_testis) groups, comprising 479 upregulated and 206 downregulated genes in the DD_ovary. Moreover, 137 miRNAs, including 83 novel and 54 known miRNAs, were detected, with 30 upregulated and 27 downregulated in the DD_ovary compared to the DD_testis. Bioinformatics analysis revealed that these miRNAs regulate key processes such as carbohydrate metabolic processes, kinase and hydrolase activity, and starch and sucrose metabolism, all potentially associated with reproductive traits. Further, key mRNA candidates, including Vitelline envelope sperm lysin receptor (Verl) and Testis-specific serine\/threonine-protein kinase (Tssk) 1, and miRNAs such as novel_90 and novel_120, were identified as components of a functional miRNA-mRNA network associated with sexual maturity and sex determination. These key genes were verified using qRT-PCR and fluorescence in situ hybridization (FISH). These transcriptomic and miRNA datasets provide valuable resources for understanding abalone reproductive biology and may support molecular breeding strategies.<\/jats:p>","DOI":"10.3390\/ijms26073235","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T05:10:07Z","timestamp":1743397807000},"page":"3235","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Integrated Analysis of mRNA and miRNA Associated with Reproduction in Female and Male Gonads in Abalone (Haliotis discus hannai)"],"prefix":"10.3390","volume":"26","author":[{"given":"Jianfang","family":"Huang","sequence":"first","affiliation":[{"name":"Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou 350108, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingcan","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenghan","family":"She","sequence":"additional","affiliation":[{"name":"Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou 350108, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianming","family":"Chen","sequence":"additional","affiliation":[{"name":"Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou 350108, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caihuan","family":"Ke","sequence":"additional","affiliation":[{"name":"College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,31]]},"reference":[{"key":"ref_1","unstructured":"Qing, W. 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