{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:07:04Z","timestamp":1760058424324,"version":"build-2065373602"},"reference-count":55,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the University of Lodz, Department of Industrial Microbiology and Biotechnology internal funds","award":["B2411000000036.01"],"award-info":[{"award-number":["B2411000000036.01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Silver nanoparticles (AgNPs) are used in a variety of different fields due to their excellent antimicrobial potential. Despite clear advantages, concerns about their toxicity have arisen, also concerning biogenic nanoparticles. Simultaneously, global healthcare is facing a problem of spreading antimicrobial resistance towards existing antibiotics. Using combined therapies involving AgNPs and antibiotics seems to be a promising solution to the above problems. The aim of this study was to evaluate the enhancement of the effectiveness of AgNPs, ciprofloxacin, and gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa. The research involved the assessment of antimicrobial and antibiofilm-forming activities and the analysis of phospholipid and fatty acid profiles. Our results showed that combining the tested antimicrobials can enhance their activity against the tested bacterial strains. However, no effect was observed while mixing AgNPs with ciprofloxacin against P. aeruginosa. The most significant effect was obtained by combining 3.125 \u00b5g\/mL of AgNPs with 0.125 \u00b5g\/mL of gentamicin against S. aureus. It was also shown that the tested antimicrobials applied in combination exhibited an increased inhibitory activity towards bacterial biofilm formation by S. aureus. Lipidomic analysis revealed that under the influence of the tested antimicrobials, the properties of the cell membrane were altered in different ways depending on the bacterial strain.<\/jats:p>","DOI":"10.3390\/ijms26083529","type":"journal-article","created":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T07:49:02Z","timestamp":1744184942000},"page":"3529","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic Activity of Gloeophyllum striatum-Derived AgNPs with Ciprofloxacin and Gentamicin Against Human Pathogenic Bacteria"],"prefix":"10.3390","volume":"26","author":[{"given":"Aleksandra","family":"To\u0144czyk","sequence":"first","affiliation":[{"name":"Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, 12\/16 Banacha Street, 90-237 Lodz, Poland"},{"name":"BioMedChem Doctoral School of University of Lodz and Lodz Institutes of Polish Academy of Sciences, 21\/23 Matejki Street, 90-237 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0785-4696","authenticated-orcid":false,"given":"Katarzyna","family":"Niedzia\u0142kowska","sequence":"additional","affiliation":[{"name":"Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, 12\/16 Banacha Street, 90-237 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5589-1555","authenticated-orcid":false,"given":"Przemys\u0142aw","family":"Bernat","sequence":"additional","affiliation":[{"name":"Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, 12\/16 Banacha Street, 90-237 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6395-570X","authenticated-orcid":false,"given":"Katarzyna","family":"Lisowska","sequence":"additional","affiliation":[{"name":"Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, 12\/16 Banacha Street, 90-237 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"111725","DOI":"10.1016\/j.inoche.2023.111725","article-title":"Synergistic antibacterial effect of Persicaria odorata synthesized silver nanoparticles with antibiotics on drug-resistant bacteria","volume":"159","author":"Hadi","year":"2024","journal-title":"Inorg. 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