{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T09:47:36Z","timestamp":1783504056310,"version":"3.55.0"},"reference-count":28,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Biological Breeding-National Science and Technology Major Project","award":["2023ZD0407504"],"award-info":[{"award-number":["2023ZD0407504"]}]},{"name":"Biological Breeding-National Science and Technology Major Project","award":["245-2022-Z1090224009"],"award-info":[{"award-number":["245-2022-Z1090224009"]}]},{"name":"Biological Breeding-National Science and Technology Major Project","award":["245-2023-F2010123001"],"award-info":[{"award-number":["245-2023-F2010123001"]}]},{"name":"Chinese Universities Scientific Fund","award":["2023ZD0407504"],"award-info":[{"award-number":["2023ZD0407504"]}]},{"name":"Chinese Universities Scientific Fund","award":["245-2022-Z1090224009"],"award-info":[{"award-number":["245-2022-Z1090224009"]}]},{"name":"Chinese Universities Scientific Fund","award":["245-2023-F2010123001"],"award-info":[{"award-number":["245-2023-F2010123001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Bovine pluripotent stem cells (PSCs) hold significant potential for diverse applications in agriculture, reproductive biotechnology, and biomedical research. However, challenges persist in establishing stable bovine PSC lines and understanding the mechanisms underlying their pluripotency maintenance. Here, we derived bovine embryonic stem cells (bESCs) from Holstein cattle embryos. These cells exhibited robust differentiation capacity into three germ layers in vitro and in vivo. Transcriptome analysis revealed distinct molecular profiles compared to primed-state bESCs. Notably, bESC proliferation ceased on methanol-treated feeder cells, in contrast to mouse ESCs (mESCs), which proliferated normally. Pathway analysis identified key signaling events critical for bESC survival and proliferation, highlighting species-specific regulatory mechanisms. Furthermore, the derived bESCs demonstrated chimerism capacity in early bovine embryos, underscoring their functional pluripotency. This work provides a foundation for advancing bovine embryology research and stem cell-based biotechnologies in livestock.<\/jats:p>","DOI":"10.3390\/ijms26083536","type":"journal-article","created":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T12:05:56Z","timestamp":1744200356000},"page":"3536","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Establishing Bovine Embryonic Stem Cells and Dissecting Their Self-Renewal Mechanisms"],"prefix":"10.3390","volume":"26","author":[{"given":"Ningxiao","family":"Li","sequence":"first","affiliation":[{"name":"Shaanxi Centre of Stem Cells Engineering & Technology, Key Laboratory of Livestock Biology, College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Yang","sequence":"additional","affiliation":[{"name":"Shaanxi Centre of Stem Cells Engineering & Technology, 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