{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:08:13Z","timestamp":1760058493889,"version":"build-2065373602"},"reference-count":35,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T00:00:00Z","timestamp":1744416000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"science and technology planning project of Guangzhou","award":["202002030019","2021A1515012402"],"award-info":[{"award-number":["202002030019","2021A1515012402"]}]},{"name":"Natural Science Foundation of Guangdong Province","award":["202002030019","2021A1515012402"],"award-info":[{"award-number":["202002030019","2021A1515012402"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The brown planthopper Nilaparvata lugens, a major rice pest, threatens global food security through rapid reproduction. This study investigates the role of the tramtrack (ttk) gene in male reproductive development and spermatogenesis using RNA interference (RNAi). Gene expression analysis revealed higher ttk levels in testes. RNAi-mediated knockdown of ttk in fourth-instar male nymphs reduced its expression by up to 80%, leading to severely impaired gonad development. Testes, vas deferens, and accessory glands in treated males exhibited 8\u201389% volume reductions compared to controls, accompanied by a 51\u201369% decline in sperm count and 60\u201385% reduction in sperm motility. Consequently, eggs fertilized by treated males showed a 73% decrease in hatching rates, with arrested embryonic development. These findings demonstrate ttk\u2019s critical role in spermatogenesis and gonad maturation in N. lugens, highlighting its potential as an RNAi target for sustainable pest control strategies.<\/jats:p>","DOI":"10.3390\/ijms26083643","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T06:18:36Z","timestamp":1744611516000},"page":"3643","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reduction in Gonad Development and Sperm Motility in Male Brown Planthopper Nilaparvata lugens via RNAi-Mediated Knockdown of tramtrack"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3603-8733","authenticated-orcid":false,"given":"Bo","family":"Feng","sequence":"first","affiliation":[{"name":"State Key Laboratory for Biocontrol and Institute of Entomology, Sun Yat-sen University, Guangzhou 510275, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biocontrol and Institute of Entomology, Sun Yat-sen University, Guangzhou 510275, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanghai","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biocontrol and Institute of Entomology, Sun Yat-sen University, Guangzhou 510275, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"197","DOI":"10.4161\/spmg.21798","article-title":"Drosophila spermiogenesis: Big things come from little packages","volume":"2","author":"Fabian","year":"2012","journal-title":"Spermatogenesis"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/S0092-8674(00)81802-4","article-title":"Control of male sexual behavior and sexual orientation in Drosophila by the fruitless gene","volume":"87","author":"Ryner","year":"1996","journal-title":"Cell"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Ueno, M., Nakata, M., Kaneko, Y., Iwami, M., Takayanagi-Kiya, S., and Kiya, T. 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