{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T20:28:04Z","timestamp":1781641684148,"version":"3.54.5"},"reference-count":57,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T00:00:00Z","timestamp":1744675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instituto Mexicano del Seguro Social","award":["R-2008-3601-113\/FIS\/IMSS\/PROT\/551"],"award-info":[{"award-number":["R-2008-3601-113\/FIS\/IMSS\/PROT\/551"]}]},{"name":"Instituto Mexicano del Seguro Social","award":["HIM\/2021\/068"],"award-info":[{"award-number":["HIM\/2021\/068"]}]},{"name":"Direction of Research at the Hospital Infantil de M\u00e9xico Federico G\u00f3mez","award":["R-2008-3601-113\/FIS\/IMSS\/PROT\/551"],"award-info":[{"award-number":["R-2008-3601-113\/FIS\/IMSS\/PROT\/551"]}]},{"name":"Direction of Research at the Hospital Infantil de M\u00e9xico Federico G\u00f3mez","award":["HIM\/2021\/068"],"award-info":[{"award-number":["HIM\/2021\/068"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Patients with end-stage renal disease (ESRD) are at increased risk of cardiovascular disease (CVD), such as myocardial infarction (MI). Uremic toxins and endothelial dysfunction are central to this process. In this exploratory study, we used the Affymetrix GeneChip microarray to investigate the gene expression profile in uremic serum-induced human coronary arterial endothelial cells (HCAECs) from ESRD patients with and without MI (UWI and UWOI groups) as an approach to its underlying mechanism. We also explored which pathways are involved in this process. We found 100 differentially expressed genes (DEGs) among the conditions of interest by supervised principal component analysis and hierarchical cluster analysis. The expressions of four major DEGs were validated by quantitative RT-PCR. Pathway analysis and molecular network were used to analyze the interaction and expression patterns. Ten pathways were identified as the main enriched metabolic pathways according to the transcriptome profiling analysis, which were, among others, positive regulation of inflammatory response, positive regulation of extracellular signal-regulated kinases 1 and 2 (ERK1\/2) cascade, cardiac muscle cell development, highlighting positive regulation of mitogen-activated protein kinase (MAPK) activity (p = 0.00016). Up- and down-regulation of genes from HCAECs exposed to uremic serum could contribute to increased endothelial dysfunction and CVD in ESRD patients. Our study suggests that inflammation and the ERK-MAPK pathway are highly enriched in kidney disease patients with MI, suggesting their role in ESRD pathology. Further studies and approaches based on MAPK pathway interfering strategies are needed to confirm these data.<\/jats:p>","DOI":"10.3390\/ijms26083732","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T09:41:11Z","timestamp":1744710071000},"page":"3732","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7040-9379","authenticated-orcid":false,"given":"Ang\u00e9lica","family":"Rangel-L\u00f3pez","sequence":"first","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades Nefrol\u00f3gicas, UMAE Hospital de Especialidades, Centro M\u00e9dico Nacional (CMN) Siglo XXI (SXXI), Instituto Mexicano del Seguro Social (IMSS), Mexico City 06720, Mexico"},{"name":"Laboratorio de Virolog\u00eda, Unidad de Investigaci\u00f3n en Enfermedades Infecciosas, Hospital Infantil de M\u00e9xico Federico G\u00f3mez-Secretar\u00eda de Salud (SS), Mexico City 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minerva","family":"Mata-Rocha","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Gen\u00e9tica Humana, UMAE Hospital de Pediatr\u00eda, CMN SXXI IMSS, Mexico City 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5672-648X","authenticated-orcid":false,"given":"Oscar Alberto","family":"P\u00e9rez-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Laboratorio de Oncolog\u00eda Experimental, Instituto Nacional de Pediatr\u00eda-SS, Mexico City 04530, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5030-5836","authenticated-orcid":false,"given":"Ricardo","family":"L\u00f3pez-Romero","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n en Biomedicina y Oncolog\u00eda Gen\u00f3mica, Hospital de Gineco-Pediatr\u00eda 3A, IMSS, Mexico City 07760, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7318-0618","authenticated-orcid":false,"given":"Dulce Mar\u00eda","family":"L\u00f3pez-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Enfermedades Infecciosas, Instituto Nacional de Enfermedades Respiratorias, SS, Mexico City 14080, Mexico"},{"name":"Laboratorio de Biotecnolog\u00eda y Bioinform\u00e1tica Gen\u00f3mica, ENCB-Instituto Polit\u00e9cnico Nacional, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Ju\u00e1rez-M\u00e9ndez","sequence":"additional","affiliation":[{"name":"Laboratorio de Oncolog\u00eda Experimental, Instituto Nacional de Pediatr\u00eda-SS, Mexico City 04530, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Villegas-Ruiz","sequence":"additional","affiliation":[{"name":"Laboratorio de Oncolog\u00eda Experimental, Instituto Nacional de Pediatr\u00eda-SS, Mexico City 04530, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1871-4311","authenticated-orcid":false,"given":"Alfonso","family":"M\u00e9ndez-Tenorio","sequence":"additional","affiliation":[{"name":"Laboratorio de Biotecnolog\u00eda y Bioinform\u00e1tica Gen\u00f3mica, ENCB-Instituto Polit\u00e9cnico Nacional, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8027-6231","authenticated-orcid":false,"given":"Juan Manuel","family":"Mej\u00eda-Aragur\u00e9","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00f3mica Funcional del C\u00e1ncer, Instituto Nacional de Medicina Gen\u00f3mica, SS, Mexico City 14610, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Orihuela-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Departamento Cl\u00ednico de Cardiolog\u00eda-UMAE Hospital de Especialidades, CMN SXXI IMSS, Mexico City 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1608-1808","authenticated-orcid":false,"given":"Cleto","family":"\u00c1lvarez-Aguilar","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias M\u00e9dicas y Biol\u00f3gicas \u201cDr. Ignacio Ch\u00e1vez\u201d, Universidad Michoacana de San Nicolas de Hidalgo, Morelia, Michoac\u00e1n 58020, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abraham","family":"Majluf-Cruz","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Hemostasia, Trombosis y Aterog\u00e9nesis, Hospital General Regional 1, IMSS, Mexico City 03103, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dante","family":"Amato","sequence":"additional","affiliation":[{"name":"Facultad de Estudios Superiores Iztacala, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City 54090, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Zavala-Vega","sequence":"additional","affiliation":[{"name":"Laboratorio Cl\u00ednico y Banco de Sangre, Instituto Nacional de Neurolog\u00eda y Neurocirug\u00eda, SS, Mexico City 14269, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Melchor-Doncel de la 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