{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:10:26Z","timestamp":1760058626494,"version":"build-2065373602"},"reference-count":29,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T00:00:00Z","timestamp":1745193600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Projects I+D+i Programa Operativo FEDER Andaluc\u00eda 2014\u20132020","award":["A-CTS.205.UGR18","P18-RT-698","CB16-10-00238","CTS-101","PI19-01372"],"award-info":[{"award-number":["A-CTS.205.UGR18","P18-RT-698","CB16-10-00238","CTS-101","PI19-01372"]}]},{"name":"PAIDI 2020, Junta de Andaluc\u00eda","award":["A-CTS.205.UGR18","P18-RT-698","CB16-10-00238","CTS-101","PI19-01372"],"award-info":[{"award-number":["A-CTS.205.UGR18","P18-RT-698","CB16-10-00238","CTS-101","PI19-01372"]}]},{"DOI":"10.13039\/501100004587","name":"ISCIII","doi-asserted-by":"publisher","award":["A-CTS.205.UGR18","P18-RT-698","CB16-10-00238","CTS-101","PI19-01372"],"award-info":[{"award-number":["A-CTS.205.UGR18","P18-RT-698","CB16-10-00238","CTS-101","PI19-01372"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Consejer\u00eda de Innovaci\u00f3n, Ciencia y Empresa, Junta de Andaluc\u00eda","award":["A-CTS.205.UGR18","P18-RT-698","CB16-10-00238","CTS-101","PI19-01372"],"award-info":[{"award-number":["A-CTS.205.UGR18","P18-RT-698","CB16-10-00238","CTS-101","PI19-01372"]}]},{"name":"Ministerio de Educaci\u00f3n, Spain","award":["A-CTS.205.UGR18","P18-RT-698","CB16-10-00238","CTS-101","PI19-01372"],"award-info":[{"award-number":["A-CTS.205.UGR18","P18-RT-698","CB16-10-00238","CTS-101","PI19-01372"]}]},{"name":"FIS, ISCIII, Spain","award":["A-CTS.205.UGR18","P18-RT-698","CB16-10-00238","CTS-101","PI19-01372"],"award-info":[{"award-number":["A-CTS.205.UGR18","P18-RT-698","CB16-10-00238","CTS-101","PI19-01372"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Melatonin is an indolamine derived from tryptophan, which is highly conserved throughout evolution, including in zebrafish, where it controls important cellular processes, such as circadian rhythms, oxidative stress, inflammation, and mitochondrial homeostasis. These functions of melatonin and its synthesis route are quite similar to those in humans. One of the most important enzymes in melatonin synthesis is acetylserotonin O-methyltransferase (ASMT), the rate-limiting enzyme, which catalyzes its final step. Due to genome duplication, zebrafish has two genes for this enzyme, asmt1 and asmt2. These genes show differential expression; asmt1 is primarily expressed in the retina and the pineal gland, and asmt2 is expressed in peripheral tissues, indicating different functions. Therefore, the aim of this work was to develop a mutant model for each asmt gene and to analyze their phenotypic effects in zebrafish. The results showed that the loss of 80% of the asmt2 gene affected melatonin concentration and consequently disrupted the sleep\/wake rhythm in larvae, decreasing by 50% the distance traveled. In contrast, the loss of asmt1 had a greater influence on the physical condition of adults, as locomotor activity decreased by 50%, and 75% showed malformations. These data reveal distinct functional roles of melatonin depending on their site of production that may affect the development of zebrafish.<\/jats:p>","DOI":"10.3390\/ijms26083912","type":"journal-article","created":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T20:42:00Z","timestamp":1745268120000},"page":"3912","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lack of asmt1 or asmt2 Yields Different Phenotypes and Malformations in Larvae to Adult Zebrafish"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1171-010X","authenticated-orcid":false,"given":"Paula","family":"Aranda-Mart\u00ednez","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Medicina, Departamento de Fisiolog\u00eda, Instituto de Biotecnolog\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria (Ibs. Granada), Hospital Cl\u00ednico Universitario San Cecilio, 18016 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5593-6733","authenticated-orcid":false,"given":"Jos\u00e9","family":"Fern\u00e1ndez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Medicina, Departamento de Fisiolog\u00eda, Instituto de Biotecnolog\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria (Ibs. Granada), Hospital Cl\u00ednico Universitario San Cecilio, 18016 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Elena","family":"D\u00edaz-Casado","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Medicina, Departamento de Fisiolog\u00eda, Instituto de Biotecnolog\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria (Ibs. Granada), Hospital Cl\u00ednico Universitario San Cecilio, 18016 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5855-0449","authenticated-orcid":false,"given":"Yolanda","family":"Ram\u00edrez-Casas","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Medicina, Departamento de Fisiolog\u00eda, Instituto de Biotecnolog\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria (Ibs. Granada), Hospital Cl\u00ednico Universitario San Cecilio, 18016 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9680-581X","authenticated-orcid":false,"given":"Mar\u00eda","family":"Mart\u00edn-Esteban\u00e9","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Medicina, Departamento de Fisiolog\u00eda, Instituto de Biotecnolog\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria (Ibs. Granada), Hospital Cl\u00ednico Universitario San Cecilio, 18016 Granada, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alba","family":"L\u00f3pez-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Medicina, Departamento de Fisiolog\u00eda, Instituto de Biotecnolog\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria (Ibs. Granada), Hospital Cl\u00ednico Universitario San Cecilio, 18016 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1256-7656","authenticated-orcid":false,"given":"Germaine","family":"Escames","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Medicina, Departamento de Fisiolog\u00eda, Instituto de Biotecnolog\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria (Ibs. Granada), Hospital Cl\u00ednico Universitario San Cecilio, 18016 Granada, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9680-1560","authenticated-orcid":false,"given":"Dar\u00edo","family":"Acu\u00f1a-Castroviejo","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Medicina, Departamento de Fisiolog\u00eda, Instituto de Biotecnolog\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria (Ibs. Granada), Hospital Cl\u00ednico Universitario San Cecilio, 18016 Granada, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"},{"name":"UGC de Laboratorios Cl\u00ednicos, Hospital Universitario Cl\u00ednico San Cecilio, 18016 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhao, D., Yu, Y., Shen, Y., Liu, Q., Zhao, Z., Sharma, R., and Reiter, R.J. (2019). Melatonin Synthesis and Function: Evolutionary History in Animals and Plants. Front. Endocrinol., 10.","DOI":"10.3389\/fendo.2019.00249"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Reiter, R.J., Sharma, R., Tan, D.X., Chuffa, L.G.A., da Silva, D.G.H., Slominski, A.T., Steinbrink, K., and Kleszczynski, K. (2024). 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