{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:57:59Z","timestamp":1787313479202,"version":"build-2736575974"},"reference-count":64,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T00:00:00Z","timestamp":1745280000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011852","name":"PMU-Research and Innovation Fund (PMU-RIF)","doi-asserted-by":"publisher","award":["2022-SEED-018"],"award-info":[{"award-number":["2022-SEED-018"]}],"id":[{"id":"10.13039\/501100011852","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Preeclampsia (PE), a pregnancy complication characterized by high blood pressure and organ damage, has been suggested to be associated with mitochondrial dysfunction, although evidence remains limited. This study aimed to investigate the activity of oxidative phosphorylation (OXPHOS) enzymes and the expression of related proteins in placental tissues from women diagnosed with early-onset preeclampsia (eoPE, &lt;34 weeks of gestation), late-onset preeclampsia (loPE, \u226534 weeks of gestation), and normotensive controls. Placental samples were analyzed using immunohistochemistry, western blotting, and enzymatic activity assays to assess the activity and expression of OXPHOS complexes. Complex I activity was increased by 80% in eoPE and 56% in loPE, with positive correlations between normalized complex I expression, gestational age at delivery (r = 0.85, p = 0.01), and birth weight (r = 0.88, p = 0.004) in loPE. Relative complex II expression in loPE showed positive correlations with pregnancy duration (r = 0.76, p = 0.03) and birth weight (r = 0.77, p = 0.03), while in controls, complex II expression correlated with pregnancy duration (r = 0.64, p = 0.03). Additionally, complex IV enzyme activity in eoPE was negatively correlated with maternal age at birth (r = \u22120.69, p = 0.03). The observed correlations highlight mitochondrial metabolism as a promising biomarker for predicting disease progression and guiding therapeutic interventions in preeclampsia. Unraveling its precise role in PE pathogenesis is critical to advancing diagnostic precision and improving maternal-fetal outcomes.<\/jats:p>","DOI":"10.3390\/ijms26093951","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T20:24:52Z","timestamp":1745353492000},"page":"3951","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Mitochondrial Oxidative Phosphorylation Alterations in Placental Tissues from Early- and Late-Onset Preeclampsia"],"prefix":"10.3390","volume":"26","author":[{"given":"Theresa","family":"Lehenauer","sequence":"first","affiliation":[{"name":"University Children\u2019s Hospital, Salzburger Landesklinken (SALK) and Paracelsus Medical University (PMU), M\u00fcllner Hauptstra\u00dfe 48, 5020 Salzburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi","family":"Jaksch-Bogensperger","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Salzburger Landesklinken (SALK) and Paracelsus Medical University (PMU), M\u00fcllner Hauptstra\u00dfe 48, 5020 Salzburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3589-4924","authenticated-orcid":false,"given":"Sara","family":"Huber","sequence":"additional","affiliation":[{"name":"Research Program for Receptor Biochemistry and Tumor Metabolism, University Children\u2019s Hospital, Salzburger Landesklinken (SALK) and Paracelsus Medical University (PMU), M\u00fcllner Hauptstra\u00dfe 48, 5020 Salzburg, 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and Paracelsus Medical University (PMU), M\u00fcllner Hauptstra\u00dfe 48, 5020 Salzburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1198-4776","authenticated-orcid":false,"given":"Barbara","family":"Kofler","sequence":"additional","affiliation":[{"name":"Research Program for Receptor Biochemistry and Tumor Metabolism, University Children\u2019s Hospital, Salzburger Landesklinken (SALK) and Paracelsus Medical University (PMU), M\u00fcllner Hauptstra\u00dfe 48, 5020 Salzburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina","family":"Neumayer","sequence":"additional","affiliation":[{"name":"Department of Pathology, Salzburger Landesklinken (SALK) and Paracelsus Medical University (PMU), M\u00fcllner Hauptstra\u00dfe 48, 5020 Salzburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Gharehbaghi","sequence":"additional","affiliation":[{"name":"Department 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requirements and the response to stress","volume":"51","author":"Vaughan","year":"2016","journal-title":"Reprod. Domest. Anim."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Marin, R., Chiarello, D.I., Abad, C., Rojas, D., Toledo, F., and Sobrevia, L. (2020). Oxidative stress and mitochondrial dysfunction in early-onset and late-onset preeclampsia. Biochim. Biophys. Acta Mol. Basis Dis., 1866.","DOI":"10.1016\/j.bbadis.2020.165961"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3479","DOI":"10.1007\/s00018-019-03104-6","article-title":"Human placenta and trophoblast development: Key molecular mechanisms and model systems","volume":"76","author":"Knofler","year":"2019","journal-title":"Cell Mol. Life Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1038\/ncpneph0035","article-title":"Mechanisms of disease: Pre-eclampsia","volume":"1","author":"Noris","year":"2005","journal-title":"Nat. Clin. Pract. Nephrol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/s11906-022-01184-7","article-title":"Mitochondrial Dysfunction in the Pathogenesis of Preeclampsia","volume":"24","author":"Hu","year":"2022","journal-title":"Curr. Hypertens. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/S0140-6736(10)60279-6","article-title":"Pre-eclampsia","volume":"376","author":"Steegers","year":"2010","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2186","DOI":"10.1007\/s43032-021-00464-y","article-title":"Placental Mitochondrial Abnormalities in Preeclampsia","volume":"28","author":"Vangrieken","year":"2021","journal-title":"Reprod. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"18109","DOI":"10.1073\/pnas.1907548116","article-title":"Noncanonical mitochondrial unfolded protein response impairs placental oxidative phosphorylation in early-onset preeclampsia","volume":"116","author":"Yung","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"S928","DOI":"10.1016\/j.ajog.2020.11.005","article-title":"Placental energy metabolism in health and disease-significance of development and implications for preeclampsia","volume":"226","author":"Aye","year":"2022","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1079\/PNS19910042","article-title":"Energy and substrate requirements of the placenta and fetus","volume":"50","author":"Hay","year":"1991","journal-title":"Proc. Nutr. Soc."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Santoro, D., Di Bella, G., Toscano, A., Musumeci, O., Buemi, M., and Piccoli, G.B. (2019). Mitochondrial Disease (MELAS Syndrome) Discovered at the Start of Pregnancy in a Patient with Advanced CKD: A Clinical and Ethical Challenge. J. Clin. Med., 8.","DOI":"10.3390\/jcm8030303"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Tang, J.X., Thompson, K., Taylor, R.W., and Olahova, M. (2020). Mitochondrial OXPHOS Biogenesis: Co-Regulation of Protein Synthesis, Import, and Assembly Pathways. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21113820"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Annesi, L., Tossetta, G., Borghi, C., and Piani, F. (2024). The Role of Xanthine Oxidase in Pregnancy Complications: A Systematic Review. Antioxidants, 13.","DOI":"10.3390\/antiox13101234"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/S0002-9394(14)72661-3","article-title":"Improved surgical treatment of familial exudative vitreoretinopathy in children","volume":"120","author":"Glazer","year":"1995","journal-title":"Am. J. Ophthalmol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1038\/s41419-018-1190-9","article-title":"Placental mitochondrial adaptations in preeclampsia associated with progression to term delivery","volume":"9","author":"Holland","year":"2018","journal-title":"Cell Death Dis."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Vishnyakova, P.A., Volodina, M.A., Tarasova, N.V., Marey, M.V., Tsvirkun, D.V., Vavina, O.V., Khodzhaeva, Z.S., Kan, N.E., Menon, R., and Vysokikh, M.Y. (2016). Mitochondrial role in adaptive response to stress conditions in preeclampsia. Sci. Rep., 6.","DOI":"10.1038\/srep32410"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Vaka, R., Deer, E., Cunningham, M., McMaster, K.M., Wallace, K., Cornelius, D.C., Amaral, L.M., and LaMarca, B. (2021). Characterization of Mitochondrial Bioenergetics in Preeclampsia. J. Clin. Med., 10.","DOI":"10.3390\/jcm10215063"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/s41580-021-00415-0","article-title":"The assembly, regulation and function of the mitochondrial respiratory chain","volume":"23","author":"Vercellino","year":"2022","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1007\/s10545-015-9831-y","article-title":"Spectrum of combined respiratory chain defects","volume":"38","author":"Mayr","year":"2015","journal-title":"J. Inherit. Metab. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1038\/s42255-023-00835-6","article-title":"Fatty acid oxidation organizes mitochondrial supercomplexes to sustain astrocytic ROS and cognition","volume":"5","author":"Agulla","year":"2023","journal-title":"Nat. Metab."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"29834","DOI":"10.1074\/jbc.M110.139493","article-title":"Evidence for physical association of mitochondrial fatty acid oxidation and oxidative phosphorylation complexes","volume":"285","author":"Wang","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e202302505","DOI":"10.26508\/lsa.202302505","article-title":"NAD+ depletion is central to placental dysfunction in an inflammatory subclass of preeclampsia","volume":"7","author":"Jahan","year":"2024","journal-title":"Life Sci. Alliance"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"13450","DOI":"10.1073\/pnas.1614947113","article-title":"Nicotinamide benefits both mothers and pups in two contrasting mouse models of preeclampsia","volume":"113","author":"Li","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Mayer-Pickel, K., Nanda, M., Gajic, M., and Cervar-Zivkovic, M. (2023). Preeclampsia and the Antiphospholipid Syndrome. Biomedicines, 11.","DOI":"10.20944\/preprints202306.1785.v1"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1186\/s13075-021-02518-7","article-title":"Evaluation of the severe preeclampsia classification criterion for antiphospholipid syndrome in a study of 40 patients","volume":"23","author":"Larosa","year":"2021","journal-title":"Arthritis Res. Ther."},{"key":"ref_26","unstructured":"Bustamante, J.G., Goyal, A., Rout, P., and Singhal, M. (2025). Antiphospholipid Syndrome. StatPearls, StatPearls Publishing."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.jmb.2006.06.057","article-title":"Mitochondrial respiratory chain supercomplexes are destabilized in Barth Syndrome patients","volume":"361","author":"McKenzie","year":"2006","journal-title":"J. Mol. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"eaav1850","DOI":"10.1126\/sciadv.aav1850","article-title":"How cardiolipin modulates the dynamics of respiratory complex I","volume":"5","author":"Jussupow","year":"2019","journal-title":"Sci. Adv."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1007\/s00404-015-3669-2","article-title":"Effects of serum from patients with early-onset pre-eclampsia, HELLP syndrome, and antiphospholipid syndrome on fatty acid oxidation in trophoblast cells","volume":"292","author":"Yu","year":"2015","journal-title":"Arch. Gynecol. Obstet."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.yexcr.2017.07.029","article-title":"Impaired mitochondrial fusion, autophagy, biogenesis and dysregulated lipid metabolism is associated with preeclampsia","volume":"359","author":"Zhou","year":"2017","journal-title":"Exp. Cell Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.placenta.2011.11.027","article-title":"Decreased mitochondrial fatty acid oxidation in placentas from women with preeclampsia","volume":"33","author":"Bartha","year":"2012","journal-title":"Placenta"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/j.cell.2004.05.008","article-title":"Disruption of mitochondrial function during apoptosis is mediated by caspase cleavage of the p75 subunit of complex I of the electron transport chain","volume":"117","author":"Ricci","year":"2004","journal-title":"Cell"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/j.cell.2008.03.032","article-title":"Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell death","volume":"133","author":"Martinvalet","year":"2008","journal-title":"Cell"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1111\/j.0105-2896.2010.00902.x","article-title":"Granzyme A activates another way to die","volume":"235","author":"Lieberman","year":"2010","journal-title":"Immunol. Rev."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"eaaz7548","DOI":"10.1126\/science.aaz7548","article-title":"Granzyme A from cytotoxic lymphocytes cleaves GSDMB to trigger pyroptosis in target cells","volume":"368","author":"Zhou","year":"2020","journal-title":"Science"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"496.e1","DOI":"10.1016\/j.ajog.2010.06.047","article-title":"Preeclampsia is associated with increased cytotoxic T-cell capacity to paternal antigens","volume":"203","author":"Visser","year":"2010","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Teran, E., Hernandez, I., Tana, L., Teran, S., Galaviz-Hernandez, C., Sosa-Macias, M., Molina, G., and Calle, A. (2018). Mitochondria and Coenzyme Q10 in the Pathogenesis of Preeclampsia. Front. Physiol., 9.","DOI":"10.3389\/fphys.2018.01561"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.ijgo.2008.11.033","article-title":"Coenzyme Q10 supplementation during pregnancy reduces the risk of pre-eclampsia","volume":"105","author":"Teran","year":"2009","journal-title":"Int. J. Gynaecol. Obstet."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/s41586-018-0846-z","article-title":"Mitochondrial complex III is essential for suppressive function of regulatory T cells","volume":"565","author":"Weinberg","year":"2019","journal-title":"Nature"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.cmet.2005.05.001","article-title":"Mitochondrial complex III is required for hypoxia-induced ROS production and cellular oxygen sensing","volume":"1","author":"Guzy","year":"2005","journal-title":"Cell Metab."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1038\/s41584-023-00963-z","article-title":"Mitochondrial fumarate implicated in inflammation","volume":"19","author":"Onuora","year":"2023","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Harber, K.J., de Goede, K.E., Verberk, S.G.S., Meinster, E., de Vries, H.E., van Weeghel, M., de Winther, M.P.J., and Van den Bossche, J. (2020). Succinate Is an Inflammation-Induced Immunoregulatory Metabolite in Macrophages. Metabolites, 10.","DOI":"10.3390\/metabo10090372"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1038\/nature11986","article-title":"Succinate is an inflammatory signal that induces IL-1beta through HIF-1alpha","volume":"496","author":"Tannahill","year":"2013","journal-title":"Nature"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Huang, H., Li, G., He, Y., Chen, J., Yan, J., Zhang, Q., Li, L., and Cai, X. (2024). Cellular succinate metabolism and signaling in inflammation: Implications for therapeutic intervention. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1404441"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4256","DOI":"10.1074\/jbc.M115.688762","article-title":"Fumarate and Succinate Regulate Expression of Hypoxia-inducible Genes via TET Enzymes","volume":"291","author":"Laukka","year":"2016","journal-title":"J. Biol. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1161\/CIRCRESAHA.116.307505","article-title":"Mitochondrial Metabolism in Aging Heart","volume":"118","author":"Lesnefsky","year":"2016","journal-title":"Circ. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2991","DOI":"10.1016\/j.celrep.2016.08.041","article-title":"Role of Mitochondrial Complex IV in Age-Dependent Obesity","volume":"16","author":"Li","year":"2016","journal-title":"Cell Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1186\/s13048-024-01427-y","article-title":"Ovarian aging: Energy metabolism of oocytes","volume":"17","author":"Bao","year":"2024","journal-title":"J. Ovarian Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1186\/s12967-025-06223-w","article-title":"The impact of mitochondrial dysfunction on ovarian aging","volume":"23","author":"Zhang","year":"2025","journal-title":"J. Transl. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e13568","DOI":"10.1111\/acel.13568","article-title":"Loss of heterochromatin and retrotransposon silencing as determinants in oocyte aging","volume":"21","author":"Rothman","year":"2022","journal-title":"Aging Cell"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1038\/s43587-024-00697-x","article-title":"Rejuvenation of aged oocyte through exposure to young follicular microenvironment","volume":"4","author":"Wang","year":"2024","journal-title":"Nat. Aging"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Feichtinger, R.G., Zimmermann, F., Mayr, J.A., Neureiter, D., Hauser-Kronberger, C., Schilling, F.H., Jones, N., Sperl, W., and Kofler, B. (2010). Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastoma. BMC Cancer, 10.","DOI":"10.1186\/1471-2407-10-149"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Aminzadeh-Gohari, S., Weber, D.D., Catalano, L., Feichtinger, R.G., Kofler, B., and Lang, R. (2020). Targeting Mitochondria in Melanoma. Biomolecules, 10.","DOI":"10.3390\/biom10101395"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Morscher, R.J., Aminzadeh-Gohari, S., Feichtinger, R.G., Mayr, J.A., Lang, R., Neureiter, D., Sperl, W., and Kofler, B. (2015). Inhibition of Neuroblastoma Tumor Growth by Ketogenic Diet and\/or Calorie Restriction in a CD1-Nu Mouse Model. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0129802"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Feichtinger, R.G., Neureiter, D., Kemmerling, R., Mayr, J.A., Kiesslich, T., and Kofler, B. (2019). Low VDAC1 Expression Is Associated with an Aggressive Phenotype and Reduced Overall Patient Survival in Cholangiocellular Carcinoma. Cells, 8.","DOI":"10.3390\/cells8060539"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1002\/ana.26293","article-title":"Variants in Mitochondrial ATP Synthase Cause Variable Neurologic Phenotypes","volume":"91","author":"Zech","year":"2022","journal-title":"Ann. Neurol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"107675","DOI":"10.1016\/j.ymgme.2023.107675","article-title":"Expanding the phenotypic and biochemical spectrum of NDUFAF3-related mitochondrial disease","volume":"140","author":"Wente","year":"2023","journal-title":"Mol. Genet. Metab."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1111\/exd.14778","article-title":"Ageing is associated with a reduction in markers of mitochondrial energy metabolism in the human epidermis","volume":"32","author":"Vidali","year":"2023","journal-title":"Exp. Dermatol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1038\/sj.bjc.6605028","article-title":"Lack of complex I is associated with oncocytic thyroid tumours","volume":"100","author":"Zimmermann","year":"2009","journal-title":"Br. J. Cancer"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Huber, S., Fitzner, T., Feichtinger, R.G., Hochmann, S., Kraus, T., Sotlar, K., Kofler, B., and Varga, M. (2023). Galanin System in the Human Bile Duct and Perihilar Cholangiocarcinoma. Cells, 12.","DOI":"10.3390\/cells12131678"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1002\/glia.22621","article-title":"Alterations of oxidative phosphorylation complexes in astrocytomas","volume":"62","author":"Feichtinger","year":"2014","journal-title":"Glia"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s00401-002-0638-1","article-title":"Severe depletion of mitochondrial DNA in spinal muscular atrophy","volume":"105","author":"Berger","year":"2003","journal-title":"Acta Neuropathol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3830","DOI":"10.1158\/0008-5472.CAN-20-0754","article-title":"Decreased Mitochondrial DNA Content Drives OXPHOS Dysregulation in Chromophobe Renal Cell Carcinoma","volume":"80","author":"Xiao","year":"2020","journal-title":"Cancer Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.ymgme.2009.06.012","article-title":"A novel mutation of the RRM2B gene in an infant with early fatal encephalomyopathy, central hypomyelination, and tubulopathy","volume":"98","author":"Plecko","year":"2009","journal-title":"Mol. 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