{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T15:28:28Z","timestamp":1767713308287,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T00:00:00Z","timestamp":1745539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hungarian National Research, Development, and Innovation Fund","award":["NKFI K139231","K134357","STIA-OTKA"],"award-info":[{"award-number":["NKFI K139231","K134357","STIA-OTKA"]}]},{"DOI":"10.13039\/501100002332","name":"Semmelweis University","doi-asserted-by":"publisher","award":["NKFI K139231","K134357","STIA-OTKA"],"award-info":[{"award-number":["NKFI K139231","K134357","STIA-OTKA"]}],"id":[{"id":"10.13039\/501100002332","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Excessive activity of the hormone angiotensin II (AngII) is known to contribute to the pathogenesis of multiple cardiovascular diseases, including atherosclerosis, vascular remodeling, and hypertension, primarily through inducing gene expression changes. In this study, we identified LMCD1 (LIM and cysteine-rich domains 1, also known as Dyxin), primarily recognized as a transcription co-factor involved in various oncogenic processes, cardiac hypertrophy, and vascular remodeling, as a potential key factor in AngII-mediated effects in vascular smooth muscle cells (VSMCs). We demonstrated that AngII upregulates LMCD1 expression in primary rat VSMCs through type 1 angiotensin receptor (AT1-R) activation, leading to calcium signaling and p38 MAPK pathway activation. Additionally, we also demonstrated in A7r5 vascular smooth muscle cells that LMCD1 protein overexpression results in enhanced cell proliferation and cell migration. Our findings provide insights into the mechanisms by which AngII mediates changes in LMCD1 expression. The elevated expression of LMCD1 enhanced the cell proliferation and migration in VSMCs in vitro experiments, suggesting that LMCD1 may be an important factor in vascular remodeling and the pathogenesis of severe cardiovascular diseases. These results raise the possibility that LMCD1 could be a promising pharmacological target in the cardiovascular dysfunctions associated with AT1-R overactivation.<\/jats:p>","DOI":"10.3390\/ijms26094053","type":"journal-article","created":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:08:24Z","timestamp":1745554104000},"page":"4053","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Role of LMCD1 in the Long-Term Effects of Angiotensin II in Vascular Smooth Muscle Cells"],"prefix":"10.3390","volume":"26","author":[{"given":"Janka Borb\u00e1la","family":"G\u00e9m","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Semmelweis University, T\u0171zolt\u00f3 utca 37-47, 1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kinga Bernadett","family":"Kov\u00e1cs","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Semmelweis University, T\u0171zolt\u00f3 utca 37-47, 1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8779-2383","authenticated-orcid":false,"given":"Szilvia","family":"Barsi","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Semmelweis University, T\u0171zolt\u00f3 utca 37-47, 1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saba","family":"Hadadnejadtehrani","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Semmelweis University, T\u0171zolt\u00f3 utca 37-47, 1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9385-2419","authenticated-orcid":false,"given":"Amir","family":"Damouni","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Semmelweis University, T\u0171zolt\u00f3 utca 37-47, 1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e1bor","family":"Turu","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Semmelweis University, T\u0171zolt\u00f3 utca 37-47, 1094 Budapest, Hungary"},{"name":"Institute of Molecular Life Sciences, Centre of Excellence of the Hungarian Academy of Sciences, HUN-REN-SU Research Centre of Natural Sciences, Magyar tud\u00f3sok k\u00f6r\u00fatja 2, 1117 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2746-9370","authenticated-orcid":false,"given":"Andr\u00e1s D\u00e1vid","family":"T\u00f3th","sequence":"additional","affiliation":[{"name":"Institute of Molecular Life Sciences, Centre of Excellence of the Hungarian Academy of Sciences, HUN-REN-SU Research Centre of Natural Sciences, Magyar tud\u00f3sok k\u00f6r\u00fatja 2, 1117 Budapest, Hungary"},{"name":"Department of Internal Medicine and Haematology, Semmelweis University, 1088 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7777-806X","authenticated-orcid":false,"given":"P\u00e9ter","family":"V\u00e1rnai","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Semmelweis University, T\u0171zolt\u00f3 utca 37-47, 1094 Budapest, Hungary"},{"name":"HUN-REN-SU Molecular Physiology Research Group, Hungarian Research Network and Semmelweis University, 1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8438-7251","authenticated-orcid":false,"given":"L\u00e1szl\u00f3","family":"Hunyady","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Semmelweis University, T\u0171zolt\u00f3 utca 37-47, 1094 Budapest, Hungary"},{"name":"Institute of Molecular Life Sciences, Centre of Excellence of the Hungarian Academy of Sciences, HUN-REN-SU Research Centre of Natural Sciences, Magyar tud\u00f3sok k\u00f6r\u00fatja 2, 1117 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e1s","family":"Balla","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Semmelweis University, T\u0171zolt\u00f3 utca 37-47, 1094 Budapest, Hungary"},{"name":"HUN-REN-SU Molecular Physiology Research Group, Hungarian Research Network and Semmelweis University, 1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1152\/physrev.00038.2017","article-title":"Angiotensin II Signal Transduction: An Update on Mechanisms of Physiology and Pathophysiology","volume":"98","author":"Forrester","year":"2018","journal-title":"Physiol. 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