{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:11:44Z","timestamp":1760058704440,"version":"build-2065373602"},"reference-count":54,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T00:00:00Z","timestamp":1745539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MINCyT","award":["1236","809","0025","J018-T1","IHMC22\/00011"],"award-info":[{"award-number":["1236","809","0025","J018-T1","IHMC22\/00011"]}]},{"name":"CONICET","award":["1236","809","0025","J018-T1","IHMC22\/00011"],"award-info":[{"award-number":["1236","809","0025","J018-T1","IHMC22\/00011"]}]},{"name":"UNCuyo","award":["1236","809","0025","J018-T1","IHMC22\/00011"],"award-info":[{"award-number":["1236","809","0025","J018-T1","IHMC22\/00011"]}]},{"name":"Carlos III Health Institute (ISCIII)","award":["1236","809","0025","J018-T1","IHMC22\/00011"],"award-info":[{"award-number":["1236","809","0025","J018-T1","IHMC22\/00011"]}]},{"name":"European Union","award":["1236","809","0025","J018-T1","IHMC22\/00011"],"award-info":[{"award-number":["1236","809","0025","J018-T1","IHMC22\/00011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>High-fat diets (HFDs) have been found to compromise male fertility, with cholesterol dysregulation being a key factor. Sterol regulatory element-binding protein 2 (SREBP2) is a crucial transcription factor that regulates cholesterol biosynthesis and uptake, playing an essential role in maintaining cholesterol homeostasis in the testes. This study investigated the dynamics of SREBP2 and cholesterol levels during rabbit spermatogenesis under HFD conditions. Our findings reveal that SREBP2 expression fluctuates throughout the seminiferous epithelium cycle. However, HFDs induce stage-specific disruptions in cholesterol balance, leading to sperm with increased membrane cholesterol, a reduced sperm count in semen analysis, impaired motility, abnormal morphology, and decreased functionality. In the control group, SREBP2 expression patterns underscored its critical role in normal spermatogenesis. Interestingly, supplementation with extra virgin olive oil (EVOO) reversed the negative effects of HFD, normalizing SREBP2 expression and cholesterol content, which improved sperm quality. These findings emphasize the importance of stage-specific analysis in understanding how dietary fat impacts male fertility and suggest that EVOO may serve as a potential nutritional intervention to protect reproductive health.<\/jats:p>","DOI":"10.3390\/ijms26094062","type":"journal-article","created":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:08:24Z","timestamp":1745554104000},"page":"4062","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cholesterol and SREBP2 Dynamics During Spermatogenesis Stages in Rabbits: Effects of High-Fat Diet and Protective Role of Extra Virgin Olive Oil"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0752-7713","authenticated-orcid":false,"given":"Mar\u00eda Virginia","family":"Avena","sequence":"first","affiliation":[{"name":"Andrological Research Laboratory of Mendoza (LIAM), IHEM, National University of Cuyo, CONICET, Mendoza M5502JMA, Argentina"},{"name":"Metabolic Diseases Laboratory (LEM), Juan Agust\u00edn Maza University, Mendoza M5519, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abi Karenina","family":"Funes","sequence":"additional","affiliation":[{"name":"Andrological Research Laboratory of Mendoza (LIAM), IHEM, National University of Cuyo, CONICET, Mendoza M5502JMA, Argentina"},{"name":"Metabolic Diseases Laboratory (LEM), Juan Agust\u00edn Maza University, Mendoza M5519, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda \u00c1ngeles","family":"Monclus","sequence":"additional","affiliation":[{"name":"Andrological Research Laboratory of Mendoza (LIAM), IHEM, National University of Cuyo, CONICET, Mendoza M5502JMA, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6709-6447","authenticated-orcid":false,"given":"Paola Vanina","family":"Boarelli","sequence":"additional","affiliation":[{"name":"Metabolic Diseases Laboratory (LEM), Juan Agust\u00edn Maza University, Mendoza M5519, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2180-1814","authenticated-orcid":false,"given":"Luis Fernando","family":"Barbisan","sequence":"additional","affiliation":[{"name":"Biosciences Institute, Department of Structural and Functional Biology, S\u00e3o Paulo State University (UNESP), Botucatu 18610-034, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Rosa","family":"Bernal-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Internal Medicine Department, Regional University Hospital of M\u00e1laga, M\u00e1laga Biomedical Research Institute (IBIMA), 29009 M\u00e1laga, Spain"},{"name":"Biomedical Research Networking Center on Obesity and Nutrition (CIBERobn), Carlos III Health Institute, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"G\u00f3mez-Huelgas","sequence":"additional","affiliation":[{"name":"Internal Medicine Department, Regional University Hospital of M\u00e1laga, M\u00e1laga Biomedical Research Institute (IBIMA), 29009 M\u00e1laga, Spain"},{"name":"Biomedical Research Networking Center on Obesity and Nutrition (CIBERobn), Carlos III Health Institute, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8830-6482","authenticated-orcid":false,"given":"Tania Estefania Saez","family":"Lancellotti","sequence":"additional","affiliation":[{"name":"Andrological Research Laboratory of Mendoza (LIAM), IHEM, National University of Cuyo, CONICET, Mendoza M5502JMA, Argentina"},{"name":"Internal Medicine Department, Regional University Hospital of M\u00e1laga, M\u00e1laga Biomedical Research Institute (IBIMA), 29009 M\u00e1laga, Spain"},{"name":"Biomedical Research Networking Center on Obesity and Nutrition (CIBERobn), Carlos III Health Institute, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8634-1396","authenticated-orcid":false,"given":"Miguel Walter","family":"Forn\u00e9s","sequence":"additional","affiliation":[{"name":"Andrological Research Laboratory of Mendoza (LIAM), IHEM, National University of Cuyo, CONICET, Mendoza M5502JMA, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4521786","DOI":"10.1155\/2019\/4521786","article-title":"Dietary Cholesterol and Lipid Overload: Impact on Male Fertility","volume":"2019","author":"Saez","year":"2019","journal-title":"Oxidative Med. 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