{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:12:14Z","timestamp":1760058734128,"version":"build-2065373602"},"reference-count":72,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,4,27]],"date-time":"2025-04-27T00:00:00Z","timestamp":1745712000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Lebanese American University\u2014President Intramural Research Fund (PIRF)","award":["I0005"],"award-info":[{"award-number":["I0005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Sodium orthovanadate is a non-selective protein tyrosine phosphatase inhibitor that can cause several types of kidney injury, including glomerulosclerosis, inflammation, and tubular damage. Cannabis is widely known for its medicinal use, and several studies have demonstrated its anti-diabetic and anti-inflammatory properties. The current study investigated the therapeutic effect of Lebanese cannabis oil extract (COE) against sodium orthovanadate-induced nephrotoxicity both in vitro and in vivo. Sprague Dawley male rats were intraperitoneally injected with 10 mg\/kg sodium orthovanadate for 10 days followed by 5 mg\/kg; 10 mg\/kg; or 20 mg\/kg intraperitoneal injection of cannabis oil extract, starting on day 4 until day 10. The body weight of the rats was monitored during the study, and clinical parameters, including serum urea, creatinine, and electrolytes, as well as kidney and heart pathology, were measured. Conditionally immortalized cultured rat podocytes were exposed to either sodium orthovanadate or selective phosphatase inhibitors, including DUSPi (DUSP1\/6 inhibitor) and SF1670 (PTEN inhibitor), in the presence or absence of cannabis oil extract. MTS and an in vitro scratch assay were used to assess podocyte cell viability and migration, respectively. Western blot analysis was used to evaluate the phosphorylation levels of AKT and p38 MAPK. Rats injected with sodium orthovanadate displayed a marked reduction in body weight and an increase in serum creatinine and urea in comparison to the control non-treated group. All doses of COE caused a significant decrease in serum urea, with a significant decrease in serum creatinine observed at a dose of 20 mg\/kg. Moreover, the COE treatment of rats injected with orthovanadate (20 mg\/kg) showed a marked reduction in renal vascular dilatation, scattered foci of acute tubular necrosis, and numerous mitoses in tubular cells compared to the sodium orthovanadate-treated group. The cell viability assay revealed that COE reversed cytotoxicity induced by sodium orthovanadate and specific phosphatase inhibitors (DUSPi and SF1670) in rat podocytes. The in vitro scratch assay showed that COE partially restored the migratory capacity of podocytes incubated with DUSPi and SF1670. Time-course and dose-dependent experiments showed that COE (1 \u03bcg\/mL) induced a significant increase in phospho-(S473)-AKT, along with a decrease in phospho (T180 + Y182) P38 levels. The current results demonstrated that Lebanese cannabis oil possesses important kidney protective effects against sodium orthovanadate-induced renal injury.<\/jats:p>","DOI":"10.3390\/ijms26094142","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T11:48:33Z","timestamp":1745840913000},"page":"4142","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic Effect of Lebanese Cannabis Oil Extract in the Management of Sodium Orthovanadate-Induced Nephrotoxicity in Rats"],"prefix":"10.3390","volume":"26","author":[{"given":"Christabel","family":"Habchy","sequence":"first","affiliation":[{"name":"Pharmaceutical Sciences Department, School of Pharmacy, Lebanese American University, Byblos P.O. Box 36, Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9610-4749","authenticated-orcid":false,"given":"Alia","family":"Khalil","sequence":"additional","affiliation":[{"name":"Gilbert and Rose-Marie Chagoury School of Medicine Room 4722, Lebanese American University, Byblos P.O. Box 36, Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9924-2496","authenticated-orcid":false,"given":"Wassim","family":"Shebaby","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Department, School of Pharmacy, Lebanese American University, Byblos P.O. Box 36, Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Bylan","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Department, School of Pharmacy, Lebanese American University, Byblos P.O. Box 36, Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marissa","family":"El Hage","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Department, School of Pharmacy, Lebanese American University, Byblos P.O. Box 36, Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5573-2469","authenticated-orcid":false,"given":"Mona","family":"Saad","sequence":"additional","affiliation":[{"name":"Gilbert and Rose-Marie Chagoury School of Medicine Room 4722, Lebanese American University, Byblos P.O. Box 36, Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9334-9847","authenticated-orcid":false,"given":"Selim","family":"Nasser","sequence":"additional","affiliation":[{"name":"Gilbert and Rose-Marie Chagoury School of Medicine Room 4722, Lebanese American University, Byblos P.O. Box 36, Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0746-3687","authenticated-orcid":false,"given":"Wissam H.","family":"Faour","sequence":"additional","affiliation":[{"name":"Gilbert and Rose-Marie Chagoury School of Medicine Room 4722, Lebanese American University, Byblos P.O. Box 36, Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamad","family":"Mroueh","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Department, School of Pharmacy, Lebanese American University, Byblos P.O. Box 36, Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.pharmthera.2010.05.006","article-title":"Common pathophysiological mechanisms of chronic kidney disease: Therapeutic perspectives","volume":"128","year":"2010","journal-title":"Pharmacol. Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"134","DOI":"10.26719\/emhj.18.032","article-title":"Ultrapure water in haemodialysis: A step towards better quality in Lebanon","volume":"25","author":"Aoun","year":"2019","journal-title":"East. Mediterr. Health J."},{"key":"ref_3","unstructured":"Goyal, A., Daneshpajouhnejad, P., Hashmi, M.F., and Bashir, K. (2024). Acute Kidney Injury. StatPearls, StatPearls Publishing LLC."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.4155\/fmc.12.103","article-title":"The potentiality of vanadium in medicinal applications","volume":"4","author":"Rehder","year":"2012","journal-title":"Future Med. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Irving, E., and Stoker, A.W. (2017). Vanadium Compounds as PTP Inhibitors. Molecules, 22.","DOI":"10.3390\/molecules22122269"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1038\/ki.1980.140","article-title":"Effects of sodium orthovanadate on whole kidney and single nephron function","volume":"18","author":"Higashi","year":"1980","journal-title":"Kidney Int."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1136\/oem.51.7.500","article-title":"Renal toxicity and arterial hypertension in rats chronically exposed to vanadate","volume":"51","author":"Boscolo","year":"1994","journal-title":"Occup. Environ. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/s12011-017-1004-4","article-title":"Ameliorated Effects of (-)-Epigallocatechin Gallate Against Toxicity Induced by Vanadium in the Kidneys of Wistar Rats","volume":"180","author":"Soussi","year":"2017","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1242\/jcs.114.13.2375","article-title":"Tumor suppressor PTEN: Modulator of cell signaling, growth, migration and apoptosis","volume":"114","author":"Yamada","year":"2001","journal-title":"J. Cell Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S0898-6568(01)00234-0","article-title":"PTEN: The down side of PI 3-kinase signalling","volume":"14","author":"Leslie","year":"2002","journal-title":"Cell Signal"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1038\/s41392-021-00571-x","article-title":"PTEN suppresses tumorigenesis by directly dephosphorylating Akt","volume":"6","author":"Bu","year":"2021","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"F1210","DOI":"10.1152\/ajprenal.00660.2011","article-title":"PTEN loss defines a TGF-\u03b2-induced tubule phenotype of failed differentiation and JNK signaling during renal fibrosis","volume":"302","author":"Lan","year":"2012","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2429","DOI":"10.1172\/JCI78945","article-title":"Phosphatidylinositol 3-kinase signaling determines kidney size","volume":"125","author":"Chen","year":"2015","journal-title":"J. Clin. Investig."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1002\/path.4508","article-title":"Loss of PTEN promotes podocyte cytoskeletal rearrangement, aggravating diabetic nephropathy","volume":"236","author":"Lin","year":"2015","journal-title":"J. Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Huang, C.-Y., and Tan, T.-H. (2012). DUSPs, to MAP kinases and beyond. Cell Biosci., 2.","DOI":"10.1186\/2045-3701-2-24"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3203","DOI":"10.1038\/sj.onc.1210412","article-title":"Differential regulation of MAP kinase signalling by dual-specificity protein phosphatases","volume":"26","author":"Owens","year":"2007","journal-title":"Oncogene"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1172\/JCI200112367","article-title":"Apoptosis in podocytes induced by TGF-beta and Smad7","volume":"108","author":"Schiffer","year":"2001","journal-title":"J. Clin. Investig."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2941","DOI":"10.1681\/ASN.2005010055","article-title":"Podocyte depletion causes glomerulosclerosis: Diphtheria toxin-induced podocyte depletion in rats expressing human diphtheria toxin receptor transgene","volume":"16","author":"Wharram","year":"2005","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"An, N., Bassil, K., Al Jowf, G.I., Steinbusch, H.W.M., Rothermel, M., de Nijs, L., and Rutten, B.P.F. (2021). Dual-specificity phosphatases in mental and neurological disorders. Prog. Neurobiol., 198.","DOI":"10.1016\/j.pneurobio.2020.101906"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1159\/000520142","article-title":"Dual-Specificity Phosphatases and Kidney Diseases","volume":"8","author":"Li","year":"2022","journal-title":"Kidney Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s00011-017-1110-y","article-title":"Transforming growth factor-\u03b21 and phosphatases modulate COX-2 protein expression and TAU phosphorylation in cultured immortalized podocytes","volume":"67","author":"Abdallah","year":"2018","journal-title":"Inflamm. Res."},{"key":"ref_22","unstructured":"Gupta, R.C. (2016). Chapter 53\u2014Cannabis sativa and Hemp. Nutraceuticals, Academic Press."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1080\/2325548X.2014.901859","article-title":"Cannabis: Evolution and Ethnobotany","volume":"2","author":"Voeks","year":"2014","journal-title":"AAG Rev. Books"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"199","DOI":"10.3989\/asclepio.1997.v49.i2.373","article-title":"Therapeutic use of Cannibis sativa L. in Arab medicine","volume":"49","author":"Lozano","year":"1997","journal-title":"Asclepio"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"113743","DOI":"10.1016\/j.jep.2020.113743","article-title":"In vivo and in vitro anti-inflammatory activity evaluation of Lebanese Cannabis sativa L. ssp","volume":"270","author":"Shebaby","year":"2021","journal-title":"indica (Lam.). J. Ethnopharmacol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Bylan, D., Khalil, A., Shebaby, W., Habchy, C., Nasser, S., Faour, W.H., and Mroueh, M. (2024). Lebanese cannabis oil extract protected against folic acid-induced kidney fibrosis in rats. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0311790"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s42238-025-00260-4","article-title":"Lebanese Cannabis sativa L. extract protects from cisplatin-induced nephrotoxicity in mice by inhibiting podocytes apoptosis","volume":"7","author":"Khalil","year":"2025","journal-title":"J. Cannabis Res."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Andre, C.M., Hausman, J.F., and Guerriero, G. (2016). Cannabis sativa: The Plant of the Thousand and One Molecules. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.00019"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.phrs.2016.01.033","article-title":"Cannabidiol, neuroprotection and neuropsychiatric disorders","volume":"112","author":"Campos","year":"2016","journal-title":"Pharmacol. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2083","DOI":"10.1002\/ptr.6400","article-title":"Cannabis sativa L. extract and cannabidiol inhibit in vitro mediators of skin inflammation and wound injury","volume":"33","author":"Sangiovanni","year":"2019","journal-title":"Phytother. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1212\/WNL.0000000000000363","article-title":"Systematic review: Efficacy and safety of medical marijuana in selected neurologic disorders: Report of the Guideline Development Subcommittee of the American Academy of Neurology","volume":"82","author":"Koppel","year":"2014","journal-title":"Neurology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.etp.2008.04.006","article-title":"The preventive effect of cannabinoids on reperfusion-induced ischemia of mouse kidney","volume":"60","author":"Feizi","year":"2008","journal-title":"Exp. Toxicol. Pathol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.lfs.2012.07.030","article-title":"Cannabidiol treatment ameliorates ischemia\/reperfusion renal injury in rats","volume":"91","author":"Fouad","year":"2012","journal-title":"Life Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Ucibior, A., Go\u0142\u0119biowska, D., Adamczyk, A., Nied\u017awiecka, I., and Fornal, E. (2014). The renal effects of vanadate exposure: Potential biomarkers and oxidative stress as a mechanism of functional renal disorders--preliminary studies. BioMed Res. Int., 2014.","DOI":"10.1155\/2014\/740105"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1038\/ki.2008.109","article-title":"Failure to phosphorylate AKT in podocytes from mice with early diabetic nephropathy promotes cell death","volume":"73","author":"Tejada","year":"2008","journal-title":"Kidney Int."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Cai, C., Hu, W., Zhang, Y., Hu, X., Yang, S., Qiu, H., Wang, R., Ma, M., Qiu, Y., and Chu, T. (2021). BCI Suppresses RANKL-Mediated Osteoclastogenesis and Alleviates Ovariectomy-Induced Bone Loss. Front. Pharmacol., 12.","DOI":"10.3389\/fphar.2021.772540"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1002\/2211-5463.13418","article-title":"The cytotoxic action of BCI is not dependent on its stated DUSP1 or DUSP6 targets in neuroblastoma cells","volume":"12","author":"Thompson","year":"2022","journal-title":"FEBS Open Bio"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Silva-Nolasco, A.M., Camacho, L., Saavedra-D\u00edaz, R.O., Hern\u00e1ndez-Abreu, O., Le\u00f3n, I.E., and S\u00e1nchez-Lombardo, I. (2020). Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity. Inorganics, 8.","DOI":"10.3390\/inorganics8120067"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"83","DOI":"10.15171\/jcvtr.2018.13","article-title":"Complete inhibition of phosphatase and tensin homolog promotes the normal and oxygen-glucose deprivation\/reperfusion-injured PC12 cells to cell death","volume":"10","author":"Rasemi","year":"2018","journal-title":"J. Cardiovasc. Thorac. Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Zhou, J., Jia, L., Hu, Z., and Wang, Y. (2017). Pharmacological Inhibition of PTEN Aggravates Acute Kidney Injury. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-10336-8"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1089\/can.2019.0102","article-title":"Neuroprotective Effect of Cannabidiol Against Hydrogen Peroxide in Hippocampal Neuron Culture","volume":"6","author":"Kim","year":"2021","journal-title":"Cannabis Cannabinoid Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2932","DOI":"10.1111\/bph.15020","article-title":"Cannabidiol protects against high glucose-induced oxidative stress and cytotoxicity in cardiac voltage-gated sodium channels","volume":"177","author":"Fouda","year":"2020","journal-title":"Br. J. Pharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Li, Y., Hao, D., Wei, D., Xiao, Y., Liu, L., Li, X., Wang, L., Gan, Y., Yan, W., and Ke, B. (2022). Photoprotective Effects of Cannabidiol against Ultraviolet-B-Induced DNA Damage and Autophagy in Human Keratinocyte Cells and Mouse Skin Tissue. Molecules, 27.","DOI":"10.3390\/molecules27196740"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Robitaille, A.C., Caron, E., Zucchini, N., Mukawera, E., Adam, D., Mariani, M.K., G\u00e9linas, A., Fortin, A., Brochiero, E., and Grandvaux, N. (2017). DUSP1 regulates apoptosis and cell migration, but not the JIP1-protected cytokine response, during Respiratory Syncytial Virus and Sendai Virus infection. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-17689-0"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1038\/onc.2010.449","article-title":"DUSP1\/MKP1 promotes angiogenesis, invasion and metastasis in non-small-cell lung cancer","volume":"30","author":"Bandres","year":"2011","journal-title":"Oncogene"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"14","DOI":"10.20892\/j.issn.2095-3941.2017.0107","article-title":"Dual-specificity phosphatase 6 (DUSP6): A review of its molecular characteristics and clinical relevance in cancer","volume":"15","author":"Ahmad","year":"2018","journal-title":"Cancer Biol. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.canlet.2017.10.007","article-title":"Pharmacological inhibition of DUSP6 suppresses gastric cancer growth and metastasis and overcomes cisplatin resistance","volume":"412","author":"Wu","year":"2018","journal-title":"Cancer Lett."},{"key":"ref_48","first-page":"182","article-title":"Long non-coding RNA FER1L4 inhibits cell proliferation and metastasis through regulation of the PI3K\/AKT signaling pathway in lung cancer cells","volume":"20","author":"Gao","year":"2019","journal-title":"Mol. Med. Rep."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"4035257","DOI":"10.1155\/2021\/4035257","article-title":"DOK7 Inhibits Cell Proliferation, Migration, and Invasion of Breast Cancer via the PI3K\/PTEN\/AKT Pathway","volume":"2021","author":"Yue","year":"2021","journal-title":"J. Oncol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.2337\/db18-0837","article-title":"Diabetes-Induced DUSP4 Reduction Promotes Podocyte Dysfunction and Progression of Diabetic Nephropathy","volume":"68","author":"Denhez","year":"2019","journal-title":"Diabetes"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2035","DOI":"10.1046\/j.1523-1755.2000.00070.x","article-title":"Regulation of mouse podocyte process dynamics by protein tyrosine phosphatases rapid communication","volume":"57","author":"Reiser","year":"2000","journal-title":"Kidney Int."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Anis, O., Vinayaka, A.C., Shalev, N., Namdar, D., Nadarajan, S., Anil, S.M., Cohen, O., Belausov, E., Ramon, J., and Mayzlish Gati, E. (2021). Cannabis-Derived Compounds Cannabichromene and \u03949-Tetrahydrocannabinol Interact and Exhibit Cytotoxic Activity against Urothelial Cell Carcinoma Correlated with Inhibition of Cell Migration and Cytoskeleton Organization. Molecules, 26.","DOI":"10.3390\/molecules26020465"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"77543","DOI":"10.18632\/oncotarget.12721","article-title":"Cannabinoids synergize with carfilzomib, reducing multiple myeloma cells viability and migration","volume":"7","author":"Nabissi","year":"2016","journal-title":"Oncotarget"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Gerasymchuk, M., Robinson, G.I., Groves, A., Haselhorst, L., Nandakumar, S., Stahl, C., Kovalchuk, O., and Kovalchuk, I. (2022). Phytocannabinoids Stimulate Rejuvenation and Prevent Cellular Senescence in Human Dermal Fibroblasts. Cells, 11.","DOI":"10.3390\/cells11233939"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1021\/acs.molpharmaceut.8b01252","article-title":"Cannabidiol Increases Proliferation, Migration, Tubulogenesis, and Integrity of Human Brain Endothelial Cells through TRPV2 Activation","volume":"16","author":"Luo","year":"2019","journal-title":"Mol. Pharm."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.fitote.2016.11.010","article-title":"Target regulation of PI3K\/Akt\/mTOR pathway by cannabidiol in treatment of experimental multiple sclerosis","volume":"116","author":"Giacoppo","year":"2017","journal-title":"Fitoterapia"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Ivanov, V.N., Grabham, P.W., Wu, C.C., and Hei, T.K. (2021). Author Correction: Inhibition of autophagic flux differently modulates cannabidiol-induced death in 2D and 3D glioblastoma cell cultures. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-98292-2"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0378-4274(98)00385-3","article-title":"Effect of age on vanadium nephrotoxicity in rats","volume":"105","author":"Granero","year":"1999","journal-title":"Toxicol. Lett."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"850","DOI":"10.5604\/01.3001.0010.4783","article-title":"The toxicity of vanadium on gastrointestinal, urinary and reproductive system, and its influence on fertility and fetuses malformations","volume":"71","author":"Wilk","year":"2017","journal-title":"Postepy Hig. Med. Dosw."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"143","DOI":"10.5487\/TR.2018.34.2.143","article-title":"Vanadate-Induced Renal cAMP and Malondialdehyde Accumulation Suppresses Alpha 1 Sodium Potassium Adenosine Triphosphatase Protein Levels","volume":"34","author":"Nakchui","year":"2018","journal-title":"Toxicol. Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"127","DOI":"10.15171\/jrip.2019.24","article-title":"The attenuative effects of oral resveratrol on renalchanges induced by vanadium injection in rats","volume":"8","author":"Zendeboodi","year":"2019","journal-title":"J. Ren. Inj. Prev."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/S0006-2952(02)01213-3","article-title":"Effect of pirfenidone against vanadate-induced kidney fibrosis in rats","volume":"64","author":"Xie","year":"2002","journal-title":"Biochem. Pharmacol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1186\/1476-511X-10-65","article-title":"The protective effect of Malva sylvestris on rat kidney damaged by vanadium","volume":"10","author":"Marouane","year":"2011","journal-title":"Lipids Health Dis."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/sj.bjp.0707442","article-title":"The diverse CB1 and CB2 receptor pharmacology of three plant cannabinoids: Delta9-tetrahydrocannabinol, cannabidiol and delta9-tetrahydrocannabivarin","volume":"153","author":"Pertwee","year":"2008","journal-title":"Br. J. Pharmacol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"116717","DOI":"10.1016\/j.jns.2020.116717","article-title":"Clinical uses of cannabis and cannabinoids in the United States","volume":"411","author":"Levinsohn","year":"2020","journal-title":"J. Neurol. Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1111\/bph.13338","article-title":"The novel, orally available and peripherally restricted selective cannabinoid CB2 receptor agonist LEI-101 prevents cisplatin-induced nephrotoxicity","volume":"173","author":"Mukhopadhyay","year":"2016","journal-title":"Br. J. Pharmacol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"E5420","DOI":"10.1073\/pnas.1419901111","article-title":"Overactive cannabinoid 1 receptor in podocytes drives type 2 diabetic nephropathy","volume":"111","author":"Jourdan","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1111\/dom.13150","article-title":"Cannabinoid-1 receptor deletion in podocytes mitigates both glomerular and tubular dysfunction in a mouse model of diabetic nephropathy","volume":"20","author":"Jourdan","year":"2018","journal-title":"Diabetes Obes. Metab."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"4371","DOI":"10.1111\/bph.14495","article-title":"Reversal of albuminuria by combined AM6545 and perindopril therapy in experimental diabetic nephropathy","volume":"175","author":"Barutta","year":"2018","journal-title":"Br. J. Pharmacol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.freeradbiomed.2009.11.022","article-title":"Cannabinoid-2 receptor limits inflammation, oxidative\/nitrosative stress, and cell death in nephropathy","volume":"48","author":"Mukhopadhyay","year":"2010","journal-title":"Free Radic. Biol. Med."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1002\/cbf.3397","article-title":"Ghrelin modulates intracellular signalling pathways that are critical for podocyte survival","volume":"37","author":"Abdallah","year":"2019","journal-title":"Cell Biochem. Funct."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1007\/s00011-020-01383-5","article-title":"Ghrelin-induced multi-organ damage in mice fed obesogenic diet","volume":"69","author":"Stephan","year":"2020","journal-title":"Inflamm. 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