{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T16:39:50Z","timestamp":1787157590687,"version":"build-2736575974"},"reference-count":62,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T00:00:00Z","timestamp":1745971200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010751","name":"FONDECYT","doi-asserted-by":"publisher","award":["1240162"],"award-info":[{"award-number":["1240162"]}],"id":[{"id":"10.13039\/501100010751","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010751","name":"FONDECYT","doi-asserted-by":"publisher","award":["3200677"],"award-info":[{"award-number":["3200677"]}],"id":[{"id":"10.13039\/501100010751","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010751","name":"FONDECYT","doi-asserted-by":"publisher","award":["ID24I10119"],"award-info":[{"award-number":["ID24I10119"]}],"id":[{"id":"10.13039\/501100010751","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FONDEF IDeA","award":["1240162"],"award-info":[{"award-number":["1240162"]}]},{"name":"FONDEF IDeA","award":["3200677"],"award-info":[{"award-number":["3200677"]}]},{"name":"FONDEF IDeA","award":["ID24I10119"],"award-info":[{"award-number":["ID24I10119"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Recessive dystrophic epidermolysis bullosa (RDEB) is a severe genetic disease caused by COL7A1 mutations. It leads to skin fragility, chronic inflammation, and impaired wound healing. The condition often results in fibrotic scarring, pseudosyndactyly, and cutaneous squamous cell carcinoma (SCC). However, current animal models fail to fully replicate chronic RDEB wounds. In this study, we used Collagen VII-hypomorphic mice (Col7a1flNeo\/flNeo) and created full-thickness wounds on their paw skin, an area prone to fibrosis due to mechanical stress. We analyzed the healing process using histology, immunofluorescence, and electron microscopy. The RDEB mice showed delayed wound closure, increased inflammation, and poor granulation tissue formation. At 30 days post-injury, we observed persistent fibrosis, with elevated levels of Collagen I, \u03b1-SMA+ myofibroblasts, and tenascin-C. These mice also had fewer intraepidermal nerve fibers, which may help explain the neuropathic pain associated with RDEB. Our model reproduces the main features of chronic RDEB wounds. It offers a useful tool for evaluating therapies aimed at reducing inflammation, fibrosis, and tumor risk in these patients.<\/jats:p>","DOI":"10.3390\/ijms26094281","type":"journal-article","created":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T09:16:12Z","timestamp":1746090972000},"page":"4281","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Paw Skin as a Translational Model for Investigating Fibrotic and Inflammatory Wound Healing Defects in Recessive Dystrophic Epidermolysis Bullosa"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0231-2468","authenticated-orcid":false,"given":"Cristian","family":"De Gregorio","sequence":"first","affiliation":[{"name":"Centro de Medicina Regenerativa, Facultad de Medicina, Cl\u00ednica Alemana-Universidad del Desarrollo, Santiago 7610658, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giselle","family":"Ramos-Gonzalez","sequence":"additional","affiliation":[{"name":"Centro de Medicina Regenerativa, Facultad de Medicina, Cl\u00ednica Alemana-Universidad del Desarrollo, Santiago 7610658, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernardo","family":"Morales-Catal\u00e1n","sequence":"additional","affiliation":[{"name":"Centro de Anatom\u00eda Patol\u00f3gica, Biolog\u00eda Molecular y Gen\u00e9tica, ATRYS, Santiago 7510249, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7696-4215","authenticated-orcid":false,"given":"Fernando","family":"Ezquer","sequence":"additional","affiliation":[{"name":"Centro de Medicina Regenerativa, Facultad de Medicina, Cl\u00ednica Alemana-Universidad del Desarrollo, Santiago 7610658, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Ezquer","sequence":"additional","affiliation":[{"name":"Centro de Medicina Regenerativa, Facultad de Medicina, Cl\u00ednica Alemana-Universidad del Desarrollo, Santiago 7610658, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/nrm2636","article-title":"Epidermal homeostasis: A balancing act of stem cells in the skin","volume":"10","author":"Blanpain","year":"2009","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Jevti\u0107, M., L\u00f6wa, A., Nov\u00e1\u010dkov\u00e1, A., Kov\u00e1\u010dik, A., Kaessmeyer, S., Erdmann, G., V\u00e1vrov\u00e1, K., and Hedtrich, S. (2020). Impact of intercellular crosstalk between epidermal keratinocytes and dermal fibroblasts on skin homeostasis. Biochim. Biophys. Acta Mol. Cell Res., 1867.","DOI":"10.1016\/j.bbamcr.2020.118722"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1089\/scd.2021.0124","article-title":"Histology Scoring System for Murine Cutaneous Wounds","volume":"30","author":"Boodhoo","year":"2021","journal-title":"Stem Cells Dev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1111\/1523-1747.ep12560795","article-title":"Type VII collagen is a normal component of epidermal basement membrane, which shows altered expression in recessive dystrophic epidermolysis bullosa","volume":"90","author":"Leigh","year":"1988","journal-title":"J. Investig. Dermatol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1016\/j.ajpath.2017.03.003","article-title":"Pathomechanisms of Altered Wound Healing in Recessive Dystrophic Epidermolysis Bullosa","volume":"187","author":"Cianfarani","year":"2017","journal-title":"Am. J. Pathol."},{"key":"ref_6","first-page":"1125","article-title":"Hereditary epidermolysis bullosa","volume":"13","author":"Laimer","year":"2015","journal-title":"J. Dtsch. Dermatol. Ges."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1016\/j.jaad.2014.01.903","article-title":"Inherited epidermolysis bullosa: Updated recommendations on diagnosis and classification","volume":"70","author":"Fine","year":"2014","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.matbio.2018.12.001","article-title":"Decorin counteracts disease progression in mice with recessive dystrophic epidermolysis bullosa","volume":"81","author":"Cianfarani","year":"2019","journal-title":"Matrix Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3498","DOI":"10.1172\/JCI68127","article-title":"Collagen VII plays a dual role in wound healing","volume":"123","author":"Velati","year":"2013","journal-title":"J. Clin. Investig."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2108","DOI":"10.1016\/j.jid.2023.05.024","article-title":"Type VII Collagen Deficiency in the Oncogenesis of Cutaneous Squamous Cell Carcinoma in Dystrophic Epidermolysis Bullosa","volume":"143","author":"South","year":"2023","journal-title":"J. Investig. Dermatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"11703","DOI":"10.1038\/ncomms11703","article-title":"Tenascin-C drives persistence of organ fibrosis","volume":"7","author":"Bhattacharyya","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1089\/wound.2013.0466","article-title":"The Role of TGFbeta Signaling in Wound Epithelialization","volume":"3","author":"Ramirez","year":"2014","journal-title":"Adv. Wound Care"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.matbio.2015.08.001","article-title":"Skin tissue repair: Matrix microenvironmental influences","volume":"49","author":"Wells","year":"2016","journal-title":"Matrix Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/s41584-019-0324-5","article-title":"Evasion of apoptosis by myofibroblasts: A hallmark of fibrotic diseases","volume":"16","author":"Hinz","year":"2020","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e165","DOI":"10.1038\/oncsis.2015.24","article-title":"Stiff substrates increase YAP-signaling-mediated matrix metalloproteinase-7 expression","volume":"4","author":"Nukuda","year":"2015","journal-title":"Oncogenesis"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1172\/JCI34292","article-title":"A hypomorphic mouse model of dystrophic epidermolysis bullosa reveals mechanisms of disease and response to fibroblast therapy","volume":"118","author":"Fritsch","year":"2008","journal-title":"J. Clin. Investig."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.15252\/emmm.201505061","article-title":"Losartan ameliorates dystrophic epidermolysis bullosa and uncovers new disease mechanisms","volume":"7","author":"Thriene","year":"2015","journal-title":"EMBO Mol. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1111\/j.1468-3083.2004.01067.x","article-title":"Vitamin and trace metal levels in recessive dystrophic epidermolysis bullosa","volume":"18","author":"Vrillat","year":"2004","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1111\/bjd.18475","article-title":"Natural history of growth and anaemia in children with epidermolysis bullosa: A retrospective cohort study","volume":"182","author":"Reimer","year":"2020","journal-title":"Br. J. Dermatol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.jdermsci.2017.12.009","article-title":"Skin wound healing in humans and mice: Challenges in translational research","volume":"90","author":"Zomer","year":"2018","journal-title":"J. Dermatol. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0140-6736(07)61128-3","article-title":"Pathogenesis and clinical features of psoriasis","volume":"370","author":"Griffiths","year":"2007","journal-title":"Lancet"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1089\/wound.2021.0126","article-title":"Skin Inflammation with a Focus on Wound Healing","volume":"12","author":"Matar","year":"2023","journal-title":"Adv. Wound Care"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Raziyeva, K., Kim, Y., Zharkinbekov, Z., Kassymbek, K., Jimi, S., and Saparov, A. (2021). Immunology of Acute and Chronic Wound Healing. Biomolecules, 11.","DOI":"10.3390\/biom11050700"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Johnson, B.Z., Stevenson, A.W., Pr\u00eale, C.M., Fear, M.W., and Wood, F.M. (2020). The Role of IL-6 in Skin Fibrosis and Cutaneous Wound Healing. Biomedicines, 8.","DOI":"10.3390\/biomedicines8050101"},{"key":"ref_25","first-page":"547","article-title":"Injury- and inflammation-driven skin fibrosis: The paradigm of epidermolysis bullosa","volume":"68\u201369","year":"2018","journal-title":"Matrix Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1615\/CritRevImmunol.v31.i2.30","article-title":"Expression and immune function of tenascin-C","volume":"31","author":"Udalova","year":"2011","journal-title":"Crit. Rev. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.semcdb.2022.03.019","article-title":"Tenascin-C in fibrosis in multiple organs: Translational implications","volume":"128","author":"Bhattacharyya","year":"2022","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1111\/j.1365-2230.2004.01428.x","article-title":"Assessment of mobility, activities and pain in different subtypes of epidermolysis bullosa","volume":"29","author":"Fine","year":"2004","journal-title":"Clin. Exp. Dermatol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1186\/s13023-024-03349-w","article-title":"Pain in recessive dystrophic epidermolysis bullosa (RDEB): Findings of the Prospective Epidermolysis Bullosa Longitudinal Evaluation Study (PEBLES)","volume":"19","author":"Jeffs","year":"2024","journal-title":"Orphanet J. Rare Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1093\/brain\/awx069","article-title":"Recessive dystrophic epidermolysis bullosa results in painful small fibre neuropathy","volume":"140","author":"Ivulic","year":"2017","journal-title":"Brain"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1002\/cne.22019","article-title":"Altered cutaneous nerve regeneration in a simian immunodeficiency virus\/macaque intracutaneous axotomy model","volume":"514","author":"Ebenezer","year":"2009","journal-title":"J. Comp. Neurol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.1097\/j.pain.0000000000002599","article-title":"Characterisation of the pathophysiology of neuropathy and sensory dysfuncion in a mouse model of recessive dystrophic epidermolysis bullosa","volume":"163","author":"Schmidt","year":"2022","journal-title":"Pain"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1038\/jidsymp.2009.5","article-title":"Possible involvement of basement membrane damage in skin photoaging","volume":"14","author":"Amano","year":"2009","journal-title":"J. Investig. Dermatol. Symp. Proc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/s12079-017-0417-3","article-title":"Restoration of the basement membrane after wounding: A hallmark of young human skin altered with aging","volume":"12","author":"Fisher","year":"2018","journal-title":"J. Cell Commun. Signal."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1111\/j.1468-3083.2011.04415.x","article-title":"Cytokines, chemokines and growth factors in wound healing","volume":"26","author":"Behm","year":"2012","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Zhao, R., Liang, H., Clarke, E., Jackson, C., and Xue, M. (2016). Inflammation in Chronic Wounds. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17122085"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Nguyen, A.V., and Soulika, A.M. (2019). The Dynamics of the Skin\u2019s Immune System. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20081811"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3907","DOI":"10.1093\/hmg\/ddu102","article-title":"Monozygotic twins discordant for recessive dystrophic epidermolysis bullosa phenotype highlight the role of TGF-beta signalling in modifying disease severity","volume":"23","author":"Odorisio","year":"2014","journal-title":"Hum. Mol. Genet."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2280","DOI":"10.1016\/j.jid.2020.02.040","article-title":"Proteomic Profiling of Fibroblasts Isolated from Chronic Wounds Identifies Disease-Relevant Signaling Pathways","volume":"140","author":"Berberich","year":"2020","journal-title":"J. Investig. Dermatol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e14392","DOI":"10.15252\/emmm.202114392","article-title":"Pro-inflammatory immunity supports fibrosis advancement in epidermolysis bullosa: Intervention with Ang-(1\u20137)","volume":"13","author":"Bernasconi","year":"2021","journal-title":"EMBO Mol. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2381","DOI":"10.1038\/jid.2014.185","article-title":"Loss of collagen VII is associated with reduced transglutaminase 2 abundance and activity","volume":"134","author":"Mack","year":"2014","journal-title":"J. Investig. Dermatol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1139\/cjpp-2016-0248","article-title":"miR-29b promotes skin wound healing and reduces excessive scar formation by inhibition of the TGF-beta1\/Smad\/CTGF signaling pathway","volume":"95","author":"Guo","year":"2017","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2704","DOI":"10.1093\/rheumatology\/keab852","article-title":"Iontophoresis of treprostinil promotes wound healing in a murine model of scleroderma-related ulcers","volume":"61","author":"Kotzki","year":"2022","journal-title":"Rheumatology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s00403-015-1614-7","article-title":"Impaired wound healing in bleomycin-induced murine scleroderma: A new model of wound retardation","volume":"308","author":"Maeda","year":"2016","journal-title":"Arch. Dermatol. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.1016\/S0002-9440(10)63066-0","article-title":"Healing of burn wounds in transgenic mice overexpressing transforming growth factor-beta 1 in the epidermis","volume":"159","author":"Yang","year":"2001","journal-title":"Am. J. Pathol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"djv293","DOI":"10.1093\/jnci\/djv293","article-title":"Suppression of TGFbeta and Angiogenesis by Type VII Collagen in Cutaneous SCC","volume":"108","author":"Martins","year":"2016","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Nystr\u00f6m, A., Bruckner-Tuderman, L., and Kiritsi, D. (2021). Dystrophic Epidermolysis Bullosa: Secondary Disease Mechanisms and Disease Modifiers. Front. Genet., 12.","DOI":"10.3389\/fgene.2021.737272"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Wong, V.W., Sorkin, M., Glotzbach, J.P., Longaker, M.T., and Gurtner, G.C. (2011). Surgical approaches to create murine models of human wound healing. J. Biomed. Biotechnol., 2011.","DOI":"10.1155\/2011\/969618"},{"key":"ref_49","first-page":"e50265","article-title":"Murine model of wound healing","volume":"75","author":"Dunn","year":"2013","journal-title":"J. Vis. Exp."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1038\/nprot.2013.002","article-title":"The mouse excisional wound splinting model, including applications for stem cell transplantation","volume":"8","author":"Wang","year":"2013","journal-title":"Nat. Protoc."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1016\/j.jid.2018.08.005","article-title":"Research Techniques Made Simple: Animal Models of Wound Healing","volume":"138","author":"Grada","year":"2018","journal-title":"J. Investig. Dermatol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1186\/s13023-022-02299-5","article-title":"The national economic burden of rare disease in the United States in 2019","volume":"17","author":"Yang","year":"2022","journal-title":"Orphanet J. Rare Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1111\/ijd.16197","article-title":"The impact of epidermolysis bullosa on the family and healthcare practitioners: A scoping review","volume":"62","author":"Chateau","year":"2023","journal-title":"Int. J. Dermatol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/bjd.21619","article-title":"Inflammatory skin diseases and wounds","volume":"187","author":"Dissemond","year":"2022","journal-title":"Br. J. Dermatol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.jdcr.2024.03.013","article-title":"Small fiber neuropathy in epidermolysis bullosa simplex","volume":"48","author":"Babakoohi","year":"2024","journal-title":"JAAD Case Rep."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1038\/s41572-020-0210-0","article-title":"Epidermolysis bullosa","volume":"6","author":"Bardhan","year":"2020","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.abd.2023.06.009","article-title":"Hereditary epidermolysis bullosa: Clinical-epidemiological profile of 278 patients at a tertiary hospital in Sao Paulo, Brazil","volume":"99","author":"Thien","year":"2024","journal-title":"An. Bras. Dermatol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1159\/000122834","article-title":"An in vivo investigation on the wound-healing effect of two medicinal herbs using an animal model with foot ulcer","volume":"41","author":"Lau","year":"2008","journal-title":"Eur. Surg. Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"118551","DOI":"10.1016\/j.molliq.2022.118551","article-title":"Betaine-urea deep eutectic solvent improves imipenem antibiotic activity","volume":"350","author":"Olivares","year":"2022","journal-title":"J. Mol. Liq."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1186\/s13287-016-0412-2","article-title":"Localization of human adipose-derived stem cells and their effect in repair of diabetic foot ulcers in rats","volume":"7","author":"Shi","year":"2016","journal-title":"Stem Cell Res. Ther."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"De Gregorio, C., Contador, D., Campero, M., Ezquer, M., and Ezquer, F. (2018). Characterization of diabetic neuropathy progression in a mouse model of type 2 diabetes mellitus. Biol. Open, 7.","DOI":"10.1242\/bio.036830"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1186\/s13287-020-01680-0","article-title":"Human adipose-derived mesenchymal stem cell-conditioned medium ameliorates polyneuropathy and foot ulceration in diabetic BKS db\/db mice","volume":"11","author":"Contador","year":"2020","journal-title":"Stem Cell Res. Ther."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/9\/4281\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:25:11Z","timestamp":1760030711000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/9\/4281"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,30]]},"references-count":62,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["ijms26094281"],"URL":"https:\/\/doi.org\/10.3390\/ijms26094281","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,30]]}}}