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H.Rob. (Asteraceae). Cytotoxic activity was tested against a panel of human adherent cancer cell lines, including breast, cervical, and oropharyngeal cancer and glioblastoma. Cell cycle analyses using flow cytometry and Hoechst 33258-propidium iodide (HOPI) fluorescent double staining were used to describe the proapoptotic property of CCF. Wound healing assessment and the Boyden chamber assay were performed to characterize the antimetastatic action of the compound. The firefly luciferase assay was applied to clarify the action of CCF on estrogenic receptors. CCF demonstrated remarkable selective growth inhibition against the HPV-18-positive human cervical cancer cell line HeLa (IC50 = 2.28 \u00b5M). The compound elicited crucial markers of apoptosis, inhibited the migration and invasion capacity of HeLa cells, and demonstrated an antiestrogenic property. Our current data indicate that the meroterpenoid scaffold presented here displays remarkable antiproliferative and antimetastatic effects on HeLa cells and can be considered a valuable model for designing further analogs targeting cervical carcinoma.<\/jats:p>","DOI":"10.3390\/ijms26104489","type":"journal-article","created":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T06:47:15Z","timestamp":1746686835000},"page":"4489","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of the Antiproliferative and Antimetastatic Properties of Centrapalus pauciflorus Meroterpenoid Centrapalus Coumarin F"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2553-8443","authenticated-orcid":false,"given":"Hazhmat","family":"Ali","sequence":"first","affiliation":[{"name":"Institute of Pharmacodynamics and Biopharmacy, University of Szeged, 6720 Szeged, Hungary"},{"name":"College of Medicine, University of Duhok, Duhok 42001, Kurdistan Region, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1740-1411","authenticated-orcid":false,"given":"Shelan","family":"Rasool","sequence":"additional","affiliation":[{"name":"Institute of Pharmacodynamics and Biopharmacy, University of Szeged, 6720 Szeged, Hungary"},{"name":"College of Medicine, University of Duhok, Duhok 42001, Kurdistan Region, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Bello","family":"Saidu","sequence":"additional","affiliation":[{"name":"Institute of Pharmacognosy, University of Szeged, 6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e9ter","family":"Germ\u00e1n","sequence":"additional","affiliation":[{"name":"Institute of Pharmacodynamics and Biopharmacy, University of Szeged, 6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6998-5632","authenticated-orcid":false,"given":"G\u00e1bor J.","family":"Szebeni","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Genomics, Core Facility, HUN-REN Biological Research Centre, 6726 Szeged, Hungary"},{"name":"Department of Internal Medicine, Hematology Centre, University of Szeged, 6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enik\u0151","family":"Szab\u00f3","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Genomics, Core Facility, HUN-REN Biological Research Centre, 6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5013-247X","authenticated-orcid":false,"given":"D\u00f3ra","family":"R\u00e9dei","sequence":"additional","affiliation":[{"name":"Institute of Pharmacognosy, University of Szeged, 6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judit","family":"Hohmann","sequence":"additional","affiliation":[{"name":"Institute of Pharmacognosy, University of Szeged, 6720 Szeged, Hungary"},{"name":"HUN-REN-USZ Biologically Active Natural Products Research Group, University of Szeged, 6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Istv\u00e1n","family":"Zupk\u00f3","sequence":"additional","affiliation":[{"name":"Institute of Pharmacodynamics and Biopharmacy, University of Szeged, 6720 Szeged, Hungary"},{"name":"Interdisciplinary Centre of Natural Products, University of Szeged, 6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"441","DOI":"10.4103\/0019-509X.200658","article-title":"Cancer and cure: A critical analysis","volume":"53","author":"Roy","year":"2016","journal-title":"Indian J. 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