{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T10:17:28Z","timestamp":1792577848416,"version":"4.5.0"},"reference-count":35,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science and Technology Council of Taiwan","award":["MOST 106-3114-B-040-001"],"award-info":[{"award-number":["MOST 106-3114-B-040-001"]}]},{"name":"National Science and Technology Council of Taiwan","award":["NSTC 111-2218-E-040-001"],"award-info":[{"award-number":["NSTC 111-2218-E-040-001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Preimplantation genetic testing for aneuploidy (PGT-A) is widely used to select euploid embryos for in vitro fertilization (IVF), but its accuracy in predicting the implantation potential for full segmental aneuploid (Seg-A) embryos remains unclear. In this study, we investigated chromosomal concordance between clinically biopsied trophectoderm (TE) and inner cell mass (ICM) in 175 donated blastocysts, which comprised\u00a0those clinically diagnosed as euploid (13), Seg-A (36), segmental mosaicism (Seg-M) (60), whole-chromosome aneuploid (Who-A) (52), and whole-chromosome mosaicism (14). Using next-generation sequencing (NGS), we found that TE\u2013ICM concordance rates were higher for euploid (85%) and Who-A (94%) embryos but significantly lower for Seg-A (25%) and Seg-M embryos (33%). For Seg-A, the euploidy rate in the ICM was 19% and the euploidy rate in the ICM was 63% for Seg-M. These low concordance rates may be due to technical and biological artifacts of PGT-A for Seg-A. Despite the significant discordance between TE and ICM, a subset of Seg-A embryos demonstrated euploidy. While clinically diagnosed euploid embryos remain the preferred choice, Seg-A embryos should be considered as having implantation potential. In particular, Seg-A results should be clearly distinguished from Who-A results and not routinely categorically discarded. Further research is required to refine the selection criteria, aided by parental karyotyping or re-biopsy, and to develop more reliable embryo assessment methods to ensure the accurate evaluation of reproductive potential and support shared decision making between doctors and patients.<\/jats:p>","DOI":"10.3390\/ijms26115284","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T11:40:32Z","timestamp":1748864432000},"page":"5284","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Assessing the Viability of Segmental Aneuploid Embryos: A Chromosomal Concordance Study of 175 Human Blastocysts"],"prefix":"10.3390","volume":"26","author":[{"given":"En-Hui","family":"Cheng","sequence":"first","affiliation":[{"name":"Genetic Diagnosis Laboratory, Lee Women\u2019s Hospital, Taichung 40652, Taiwan"},{"name":"Post Baccalaureate Medicine, National Chung Hsing University, Taichung 40227, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8503-4802","authenticated-orcid":false,"given":"Hui-Hsin","family":"Shih","sequence":"additional","affiliation":[{"name":"Genetic Diagnosis Laboratory, Lee Women\u2019s Hospital, Taichung 40652, Taiwan"},{"name":"Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8858-0916","authenticated-orcid":false,"given":"Tsung-Hsien","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan"},{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40402, Taiwan"},{"name":"Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung 40201, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4602-5732","authenticated-orcid":false,"given":"Pin-Yao","family":"Lin","sequence":"additional","affiliation":[{"name":"Post Baccalaureate Medicine, National Chung Hsing University, Taichung 40227, Taiwan"},{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9253-7037","authenticated-orcid":false,"given":"Tzu-Ning","family":"Yu","sequence":"additional","affiliation":[{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2649-2384","authenticated-orcid":false,"given":"Chun-Chia","family":"Huang","sequence":"additional","affiliation":[{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maw-Sheng","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan"},{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40402, Taiwan"},{"name":"Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung 40201, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1310-2350","authenticated-orcid":false,"given":"Chun-I","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40402, Taiwan"},{"name":"Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung 40201, Taiwan"},{"name":"Department of Obstetrics and Gynecology, School of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1093\/humrep\/deaa034","article-title":"The status of preimplantation genetic testing in the UK and USA","volume":"35","author":"Theobald","year":"2020","journal-title":"Hum. 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