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FONDECYT de Iniciaci\u00f3n","award":["11251077"],"award-info":[{"award-number":["11251077"]}]},{"name":"ANID FONDECYT de Iniciaci\u00f3n","award":["SIP-20242196"],"award-info":[{"award-number":["SIP-20242196"]}]},{"name":"ANID FONDECYT de Iniciaci\u00f3n","award":["SIP-20241409"],"award-info":[{"award-number":["SIP-20241409"]}]},{"name":"Secretaria de Investigaci\u00f3n y Posgrado IPN","award":["1221362"],"award-info":[{"award-number":["1221362"]}]},{"name":"Secretaria de Investigaci\u00f3n y Posgrado IPN","award":["NoSA77210087"],"award-info":[{"award-number":["NoSA77210087"]}]},{"name":"Secretaria de Investigaci\u00f3n y Posgrado IPN","award":["11251077"],"award-info":[{"award-number":["11251077"]}]},{"name":"Secretaria de Investigaci\u00f3n y Posgrado IPN","award":["SIP-20242196"],"award-info":[{"award-number":["SIP-20242196"]}]},{"name":"Secretaria de Investigaci\u00f3n y Posgrado IPN","award":["SIP-20241409"],"award-info":[{"award-number":["SIP-20241409"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>PLAC8, expressed by interstitial extravillous trophoblasts (iEVTs), plays a crucial role in trophoblast invasion, differentiation, and immunotolerance. Its dysregulation may contribute to pregnancy complications, such as preeclampsia. This study investigates the role of PLAC8 in trophoblast invasiveness and endothelial-like differentiation under different oxygen tensions. Swan-71 cells were transiently transfected with PLAC8 overexpression or knockdown plasmids. Invasion was assessed using Matrigel-coated transwells, endothelial-like differentiation through tube formation assays, and vasculogenic marker expression (VEGF, PGF, ANGPT2) by RT-PCR. Hypoxia experiments were performed at different oxygen conditions. PLAC8 overexpression enhanced trophoblast invasion but reduced endothelial-like differentiation, downregulating VEGF and PGF while upregulating ANGPT2. Hypoxia increased PLAC8 expression, indicating oxygen tension as a regulatory factor. PLAC8 manipulation did not affect cell viability. PLAC8 modulates trophoblast behavior by promoting invasion while inhibiting endothelial-like differentiation. Its regulation of vasculogenic and angiogenic factors suggests a critical role in placental homeostasis and potential relevance to pregnancy disorders, such as preeclampsia.<\/jats:p>","DOI":"10.3390\/ijms26115371","type":"journal-article","created":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T03:57:34Z","timestamp":1749009454000},"page":"5371","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["PLAC8 Expression Regulates Trophoblast Invasion and Conversion into an Endothelial Phenotype (eEVT)"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9925-6668","authenticated-orcid":false,"given":"Laura J.","family":"Barrag\u00e1n-Z\u00fa\u00f1iga","sequence":"first","affiliation":[{"name":"Instituto Polit\u00e9cnico Nacional, CIIDIR-Durango, Durango 34220, Mexico"},{"name":"Centro Estatal de Cancerolog\u00eda, Secretaria de Salud de Durango, Durango 34000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0439-2263","authenticated-orcid":false,"given":"Rodrigo","family":"Escalona-Rivano","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Science, Universidad San Sebasti\u00e1n, Santiago 7510602, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catalina","family":"Cordero-Tirado","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Science, Universidad San Sebasti\u00e1n, Santiago 7510602, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha","family":"Sosa-Mac\u00edas","sequence":"additional","affiliation":[{"name":"Instituto Polit\u00e9cnico Nacional, CIIDIR-Durango, Durango 34220, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1020-734X","authenticated-orcid":false,"given":"Ivo","family":"Carrasco-Wong","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Science, Universidad San Sebasti\u00e1n, Santiago 7510602, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Science, Universidad San Sebasti\u00e1n, Santiago 7510602, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Galaviz-Hernandez","sequence":"additional","affiliation":[{"name":"Instituto Polit\u00e9cnico Nacional, CIIDIR-Durango, Durango 34220, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1159\/000443636","article-title":"The Placenta as an Organ and a Source of Stem Cells and Extracellular Matrix: A Review","volume":"201","author":"Lobo","year":"2016","journal-title":"Cells Tissues Organs"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1161\/CIRCRESAHA.118.313276","article-title":"Compendium on the Pathophysiology and Treatment of Hypertension Preeclampsia","volume":"124","author":"Rana","year":"2019","journal-title":"Circ. 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