{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:43:58Z","timestamp":1790210638504,"version":"4.1.0"},"reference-count":54,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Research Support Foundation of the State of Minas Gerais","doi-asserted-by":"publisher","award":["APQ-01254-21"],"award-info":[{"award-number":["APQ-01254-21"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Research Support Foundation of the State of Minas Gerais","doi-asserted-by":"publisher","award":["403193\/2022-2"],"award-info":[{"award-number":["403193\/2022-2"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Research Support Foundation of the State of Minas Gerais","doi-asserted-by":"publisher","award":["APQ-03613-17"],"award-info":[{"award-number":["APQ-03613-17"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Coordination for the Improvement of Higher Education Personnel (CAPES) and INCT in Theranostics and Nanobiotechnology","doi-asserted-by":"publisher","award":["APQ-01254-21"],"award-info":[{"award-number":["APQ-01254-21"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Coordination for the Improvement of Higher Education Personnel (CAPES) and INCT in Theranostics and Nanobiotechnology","doi-asserted-by":"publisher","award":["403193\/2022-2"],"award-info":[{"award-number":["403193\/2022-2"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Coordination for the Improvement of Higher Education Personnel (CAPES) and INCT in Theranostics and Nanobiotechnology","doi-asserted-by":"publisher","award":["APQ-03613-17"],"award-info":[{"award-number":["APQ-03613-17"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The increasing number of individuals with chronic kidney disease (CKD), mainly due to lifestyle changes\u2014such as increased consumption of processed foods, physical inactivity, obesity, and smoking habits\u2014and population aging, highlights the need to identify new biomarkers to facilitate monitoring of CKD progression and, consequently, predict end-stage renal disease (ESRD). This study aimed to analyze the proteomic profile of urine samples from healthy individuals and those with ESRD to identify potential biomarkers for this advanced stage of CKD. Urine samples were collected from 20 participants, comprising 10 healthy individuals and 10 patients with ESRD, and analyzed via liquid chromatography coupled with a tandem mass spectrometer. Bioinformatics analyses, including gene ontology and protein interaction, were subsequently conducted. A total of 416 proteins were identified in the proteomic profiles of the groups, and 19 proteins showed statistically significant differences between them. Of these, five proteins\u2014hemopexin, beta-2-microglobulin, retinol-binding protein 4, transthyretin, and factor D\u2014emerged as potential biomarkers for ESRD. The proteins identified were able to characterize and differentiate the urinary proteomic profiles of the two groups. The five selected proteins represent promising candidates for ESRD biomarkers.<\/jats:p>","DOI":"10.3390\/ijms26125429","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T03:52:28Z","timestamp":1749181948000},"page":"5429","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Urine Proteomics for Detection of Potential Biomarkers for End-Stage Renal Disease"],"prefix":"10.3390","volume":"26","author":[{"given":"Nathalia R.","family":"Silva","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38402-293, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca U.","family":"Picolo","sequence":"additional","affiliation":[{"name":"Nanobiotechnology Laboratory Prof. Luiz Ricardo Goulart Filho, Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38405-319, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia C. M.","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38402-293, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta S.","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38402-293, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard C.","family":"Polveiro","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38410-337, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hebr\u00e9ia O.","family":"Almeida-Souza","sequence":"additional","affiliation":[{"name":"Nanobiotechnology Laboratory Prof. Luiz Ricardo Goulart Filho, Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38405-319, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7381-2788","authenticated-orcid":false,"given":"M\u00e1rio M.","family":"Martins","sequence":"additional","affiliation":[{"name":"Nanobiotechnology Laboratory Prof. Luiz Ricardo Goulart Filho, Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38405-319, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1803-4861","authenticated-orcid":false,"given":"Luiz R.","family":"Goulart Filho","sequence":"additional","affiliation":[{"name":"Nanobiotechnology Laboratory Prof. Luiz Ricardo Goulart Filho, Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38405-319, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana S.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38402-293, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e200044","DOI":"10.1590\/1980-549720200044","article-title":"Factors associated with chronic kidney disease: Epidemiological survey of the National Health Survey","volume":"23","author":"Aguiar","year":"2020","journal-title":"Rev. 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