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The natural accumulation of omega-3 fatty acids in cell membranes is crucial for maintaining the membrane structure, supporting interactions with the ECS, and restoring synaptic plasticity and cognition impaired by prenatal ethanol (EtOH) exposure. However, it remains unclear whether omega-3 supplementation can mitigate the long-term effects on the ECS, endocannabinoid-dependent synaptic plasticity, and cognition following adolescent binge drinking. Here, we demonstrated that omega-3 supplementation during EtOH withdrawal increases CB1 receptors in hippocampal presynaptic terminals of male mice, along with the recovery of receptor-stimulated [35S]GTP\u03b3S binding to G\u03b1i\/o proteins. These changes are associated with long-term potentiation (LTP) at excitatory medial perforant path (MPP) synapses in the dentate gyrus (DG), which depends on anandamide (AEA), transient receptor potential vanilloid 1 (TRPV1), and N-methyl-D-aspartate (NMDA) receptors. Finally, omega-3 intake following binge drinking reduced the time and number of errors required to locate the escape box in the Barnes maze test. Collectively, these findings suggest that omega-3 supplementation restores Barnes maze performance to levels comparable to those of control mice after adolescent binge drinking. This recovery is likely mediated by modulation of the hippocampal ECS, enhancing endocannabinoid-dependent excitatory synaptic plasticity.<\/jats:p>","DOI":"10.3390\/ijms26125507","type":"journal-article","created":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T06:46:01Z","timestamp":1749451561000},"page":"5507","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Omega-3 Fatty Acids Mitigate Long-Lasting Disruption of the Endocannabinoid System in the Adult Mouse Hippocampus Following Adolescent Binge Drinking"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8296-9231","authenticated-orcid":false,"given":"Maitane","family":"Serrano","sequence":"first","affiliation":[{"name":"Department of Neurosciences, Faculty of Medicine and Nursing, University of the Basque Country UPV\/EHU, 48940 Leioa, Spain"},{"name":"Achucarro Basque Center for Neuroscience, Science Park of the 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(2017). The burden of binge and heavy drinking on the brain: Effects on adolescent and young adult neural structure and function. Front. Psychol., 8.","DOI":"10.3389\/fpsyg.2017.01111"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Kang, E., Yun, B., Cha, J., Suk, H.I., and Shin, E.K. (2024). Neurodevelopmental imprints of sociomarkers in adolescent brain connectomes. Sci. Rep., 14.","DOI":"10.1038\/s41598-024-71309-2"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1038\/s41386-019-0530-5","article-title":"Intermittent ethanol exposure during adolescence impairs cannabinoid type 1 receptor-dependent long-term depression and recognition memory in adult mice","volume":"45","author":"Puente","year":"2020","journal-title":"Neuropsychopharmacology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1111\/acer.14306","article-title":"Interactions between alcohol and the endocannabinoid system","volume":"44","author":"Kunos","year":"2020","journal-title":"Alcohol Clin. Exp. Res."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Navarrete, F., Garc\u00eda-Guti\u00e9rrez, M.S., Gasparyan, A., Navarro, D., L\u00f3pez-Pic\u00f3n, F., Morcuende, A., Femen\u00eda, T., and Manzanares, J. (2022). Biomarkers of the endocannabinoid system in substance use disorders. Biomolecules, 12.","DOI":"10.3390\/biom12030396"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Mar\u00edn, L., Flores-L\u00f3pez, M., Pastor, A., Gavito, A.L., Su\u00e1rez, J., de la Torre, R., Pav\u00f3n, F.J., Rodr\u00edguez de Fonseca, F., and Serrano, A. (2022). Acute stress and alcohol exposure during adolescence result in an anxious phenotype in adulthood: Role of altered glutamate\/endocannabinoid transmission mechanisms. Prog. Neuropsychopharmacol. Biol. Psychiatry, 113.","DOI":"10.1016\/j.pnpbp.2021.110460"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"03","DOI":"10.35946\/arcr.v42.1.03","article-title":"The synaptic interactions of alcohol and the endogenous cannabinoid system","volume":"42","author":"Wolfe","year":"2022","journal-title":"Alcohol Res. Curr. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1152\/physrev.00019.2008","article-title":"Endocannabinoid-mediated control of synaptic transmission","volume":"89","author":"Kano","year":"2009","journal-title":"Physiol. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e146861","DOI":"10.1172\/JCI146861","article-title":"Targeting diacylglycerol lipase reduces alcohol consumption in preclinical models","volume":"131","author":"Winters","year":"2021","journal-title":"J. Clin. Investig."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1080\/00952990.2025.2456499","article-title":"Changes in peripheral endocannabinoid levels in substance use disorders: A review of clinical evidence","volume":"51","author":"Elliott","year":"2025","journal-title":"Am. J. Drug Alcohol Abus."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"09","DOI":"10.35946\/arcr.v42.1.09","article-title":"Alcohol-endocannabinoid interactions: Implications for addiction-related behavioral processes","volume":"42","author":"Serrano","year":"2022","journal-title":"Alcohol Res. Curr. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1046\/j.1471-4159.2003.01576.x","article-title":"Cannabinoid CB1 receptor knockout mice exhibit markedly reduced voluntary alcohol consumption and lack alcohol-induced dopamine release in the nucleus accumbens","volume":"84","author":"Hungund","year":"2003","journal-title":"J. Neurochem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"55","DOI":"10.2174\/187152710790966623","article-title":"Blockade of the cannabinoid CB1 receptor and alcohol dependence: Preclinical evidence and preliminary clinical data","volume":"9","author":"Maccioni","year":"2010","journal-title":"CNS Neurol. Disord. Drug Targets"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Rico-Barrio, I., Pe\u00f1asco, S., Lekunberri, L., Serrano, M., Ega\u00f1a-Huguet, J., Mimenza, A., Soria-Gomez, E., Ramos, A., Buceta, I., and Gerrikagoitia, I. (2021). Environmental enrichment rescues endocannabinoid-dependent synaptic plasticity lost in young adult male mice after ethanol exposure during adolescence. Biomedicines, 9.","DOI":"10.3390\/biomedicines9070825"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1002\/fsn3.121","article-title":"Long-chain polyunsaturated fatty acid sources and evaluation of their nutritional and functional properties","volume":"2","author":"Abedi","year":"2014","journal-title":"Food. Sci. Nutr."},{"key":"ref_16","first-page":"323","article-title":"Importance of a balanced omega-6\/omega-3 ratio for the maintenance of health: Nutritional recommendations","volume":"26","year":"2011","journal-title":"Nutr. Hosp."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Cutuli, D., Pagani, M., Caporali, P., Galbusera, A., Laricchiuta, D., Foti, F., Neri, C., Spalleta, G., Caltagirone, C., and Petrosini, L. (2016). Effects of omega-3 fatty acid supplementation on cognitive functions and neural substrates: A voxel-based morphometry study in aged mice. Front. Aging Neurosci., 8.","DOI":"10.3389\/fnagi.2016.00038"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.neulet.2006.06.058","article-title":"Elevated oxidation of docosahexaenoic acid, 22:6 (n-3), in brain regions of rats undergoing ethanol withdrawal","volume":"405","author":"Milne","year":"2006","journal-title":"Neurosci. Lett."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Tajuddin, N., Moon, K.H., Marshall, S.A., Nixon, K., Neafsey, E.J., Kim, H.Y., and Collins, M.A. (2014). Neuroinflammation and neurodegeneration in adult rat brain from binge ethanol exposure: Abrogation by docosahexaenoic acid. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0101223"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Joffre, C., Rey, C., and Lay\u00e9, S. (2019). N-3 polyunsaturated fatty acids and the resolution of neuroinflammation. Front. Pharmacol., 10.","DOI":"10.3389\/fphar.2019.01022"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.neulet.2013.05.051","article-title":"Omega-3 fatty acids can reverse the long-term deficits in hippocampal synaptic plasticity caused by prenatal ethanol exposure","volume":"551","author":"Patten","year":"2013","journal-title":"Neurosci. Lett."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Serrano, M., Rico-Barrio, I., and Grandes, P. (2023). The effect of omega-3 fatty acids on alcohol-induced damage. Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1068343"},{"key":"ref_23","first-page":"nuaf036","article-title":"Impact of omega-3 polyunsaturated fatty acids on alcohol use and negative consequences: A systematic review","volume":"26","author":"Cardona","year":"2025","journal-title":"Nutr. Rev."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6851","DOI":"10.1523\/JNEUROSCI.3516-16.2017","article-title":"Amplification of mGlu5-endocannabinoid signaling rescues behavioral and synaptic deficits in a mouse model of adolescent and adult dietary polyunsaturated fatty acid imbalance","volume":"37","author":"Manduca","year":"2017","journal-title":"J. Neurosci."},{"key":"ref_25","first-page":"2571","article-title":"Nutritional n-3 PUFA deficiency abolishes endocannabinoid gating of hippocampal long-term potentiation","volume":"27","author":"Thomazeau","year":"2017","journal-title":"Cereb. Cortex"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Mart\u00edn-Llorente, A., Serrano, M., Bonilla-Del R\u00edo, I., Lekunberri, L., Ocerin, G., Puente, N., Ramos, A., Rico-Barrio, I., Gerrikagoitia, I., and Grandes, P. (2023). Omega-3 recovers cannabinoid 1 receptor expression in the adult mouse brain after adolescent binge drinking. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242417316"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1111\/adb.12585","article-title":"Adolescent ethanol intake alters cannabinoid type-1 receptor localization in astrocytes of the adult mouse hippocampus","volume":"24","author":"Puente","year":"2019","journal-title":"Addict. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Serrano, M., Saumell-Esnaola, M., Ocerin, G., Garc\u00eda del Ca\u00f1o, G., Puente, N., Sall\u00e9s, J., Rodr\u00edguez de Fonseca, F., Rodr\u00edguez-Arias, M., Gerrikagoitia, I., and Grandes, P. (2024). Impact of omega-3 on endocannabinoid system expression and function, enhancing cognition and behavior in male mice. Nutrients, 16.","DOI":"10.3390\/nu16244344"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.neuropharm.2019.04.020","article-title":"Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus","volume":"153","author":"Puente","year":"2019","journal-title":"Neuropharmacology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1038\/s41386-018-0055-3","article-title":"Deficient endocannabinoid signaling in the central amygdala contributes to alcohol dependence-related anxiety-like behavior and excessive alcohol intake","volume":"43","author":"Serrano","year":"2018","journal-title":"Neuropsychopharmacology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1111\/adb.12573","article-title":"Inhibition of fatty acid amide hydrolase in the central amygdala alleviates co-morbid expression of innate anxiety and excessive alcohol intake","volume":"23","author":"Stopponi","year":"2018","journal-title":"Addict. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1089\/neu.2018.5854","article-title":"A novel role for the endocannabinoid system in ameliorating motivation for alcohol drinking and negative behavioral affect after traumatic brain injury in rats","volume":"36","author":"Fucich","year":"2019","journal-title":"J. Neurotrauma"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Baos, A., Alegre-Zurano, L., Cantacorps, L., Mart\u00edn-S\u00e1nchez, A., and Valverde, O. (2021). Role of cannabinoids in alcohol-induced neuroinflammation. Prog. Neuropsychopharmacol. Biol. Psychiatry, 104.","DOI":"10.1016\/j.pnpbp.2020.110054"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"107462","DOI":"10.1016\/j.phrs.2024.107462","article-title":"Excessive alcohol intake produces persistent mechanical allodynia and dysregulates the endocannabinoid system in the lumbar dorsal root ganglia of genetically-selected Marchigian Sardinian alcohol-preferring rats","volume":"209","author":"Borgonetti","year":"2024","journal-title":"Pharmacol. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1111\/j.1530-0277.2007.00366.x","article-title":"Bidirectional alterations of hippocampal cannabinoid 1 receptors and their endogenous ligands in a rat model of alcohol withdrawal and dependence","volume":"31","author":"Mitrirattanakul","year":"2007","journal-title":"Alcohol Clin. Exp. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2991","DOI":"10.1523\/JNEUROSCI.4872-05.2006","article-title":"The CB1 cannabinoid receptor is the major cannabinoid receptor at excitatory presynaptic sites in the hippocampus and cerebellum","volume":"26","author":"Kawamura","year":"2006","journal-title":"J. Neurosci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.alcohol.2012.01.002","article-title":"A Review of the interactions between alcohol and the endocannabinoid system: Implications for alcohol dependence and future directions for research","volume":"46","author":"Pava","year":"2012","journal-title":"Alcohol"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Liput, D.J., Pauly, J.R., Stinchcomb, A.L., and Nixon, K. (2017). Binge alcohol exposure transiently changes the endocannabinoid system: A potential target to prevent alcohol-induced neurodegeneration. Brain Sci., 7.","DOI":"10.3390\/brainsci7120158"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1111\/j.1369-1600.2010.00299.x","article-title":"Innate difference in the endocannabinoid signaling and its modulation by alcohol consumption in alcohol-preferring sP rats","volume":"17","author":"Vinod","year":"2012","journal-title":"Addict. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Oliver, E.E., Hughes, E.K., Puckett, M.K., Chen, R., Lowther, W.T., and Howlett, A.C. (2020). Cannabinoid receptor interacting protein 1a (CRIP1a) in health and disease. Biomolecules, 10.","DOI":"10.3390\/biom10121609"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s00232-021-00197-8","article-title":"Cholesterol in GPCR Structures: Prevalence and relevance","volume":"255","author":"Sarkar","year":"2022","journal-title":"J. Membr. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Jakubik, J., and El-Fakahany, E.E. (2021). Allosteric modulation of GPCRs of class A by cholesterol. Int. J. Mol. Sci., 22.","DOI":"10.20944\/preprints202101.0409.v1"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Carvajal, F., S\u00e1nchez-Gil, A., Cardona, D., Rinc\u00f3n-Cervera, M.A., and Lerma-Cabrera, J.M. (2023). The effect of very-long-chain n-3 polyunsaturated fatty acids in the central nervous system and their potential benefits for treating alcohol use disorder: Reviewing pre-clinical and clinical data. Nutrients, 15.","DOI":"10.3390\/nu15132993"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.biopsych.2008.02.020","article-title":"Antistress effect of TRPV1 channel on synaptic plasticity and spatial memory","volume":"64","author":"Li","year":"2008","journal-title":"Biol. Psychiatry"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1523\/JNEUROSCI.3303-06.2007","article-title":"Reduced anxiety, conditioned fear, and hippocampal long-term potentiation in transient receptor potential vanilloid type 1 receptor-deficient mice","volume":"27","author":"Marsch","year":"2007","journal-title":"J. Neurosci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1523\/JNEUROSCI.0257-19.2019","article-title":"suppression of presynaptic glutamate release by postsynaptic metabotropic NMDA receptor signalling to pannexin-1","volume":"40","author":"Bialecki","year":"2020","journal-title":"J. Neurosci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1113\/jphysiol.2006.121988","article-title":"TRPV1 is a novel target for omega-3 polyunsaturated fatty acids","volume":"578","author":"Matta","year":"2007","journal-title":"J. Physiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1101\/lm.046722.117","article-title":"Synaptic plasticity mechanisms common to learning and alcohol use disorder","volume":"25","author":"Lovinger","year":"2018","journal-title":"Learn Mem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2763","DOI":"10.1111\/acer.12542","article-title":"Ethanol attenuation of long-term depression in the nucleus accumbens can be overcome by activation of TRPV1 receptors","volume":"38","author":"Renteria","year":"2014","journal-title":"Alcohol Clin. Exp. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"6350","DOI":"10.1523\/JNEUROSCI.3786-12.2013","article-title":"Anandamide-CB1 receptor signaling contributes to postnatal ethanol-induced neonatal neurodegeneration, adult synaptic, and memory deficits","volume":"33","author":"Subbanna","year":"2013","journal-title":"J. Neurosci."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Drissi, I., Deschamps, C., Fouquet, G., Alary, R., Peineau, S., Gosset, P., Sueur, H., Marcq, I., Debuysscher, S., and Naassila, M. (2020). Memory and plasticity impairment after binge drinking in adolescent rat hippocampus: GluN2A\/GluN2B NMDA receptor subunits imbalance through HDAC2. Addict. Biol., 25.","DOI":"10.1111\/adb.12760"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2398212819848213","DOI":"10.1177\/2398212819848213","article-title":"Persistent memories of long-term potentiation and the N-methyl-D-aspartate receptor","volume":"3","author":"Bliss","year":"2019","journal-title":"Brain Neurosci. Adv."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1113\/jphysiol.1994.sp020051","article-title":"Facilitatory effect of docosahexaenoic acid on N-methyl-D-aspartate response in pyramidal neurones of rat cerebral cortex","volume":"475","author":"Nishikawa","year":"1994","journal-title":"J. Physiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1016\/j.jnutbio.2012.04.003","article-title":"Omega-3 supplementation can restore glutathione levels and prevent oxidative damage caused by prenatal ethanol exposure","volume":"24","author":"Patten","year":"2013","journal-title":"J. Nutr. Biochem."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Isaac, A.R., Coelho de Velasco, P., Dias Fraga, K.Y., Tavares-do-Carmo, M.G., Pereira Campos, R.M., Iannotti, F.A., Verde, R., Gondim Martins, D.B., Aragao Santos, T., and Klippel Ferreira, B. (2021). Maternal omega-3 intake differentially affects the endocannabinoid system in the progeny\u2019s neocortex and hippocampus: Impact on synaptic markers. J. Nutr. Biochem., 96.","DOI":"10.1016\/j.jnutbio.2021.108782"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1007\/s00429-014-0711-2","article-title":"The transient receptor potential vanilloid-1 is localized at excitatory synapses in the mouse dentate gyrus","volume":"220","author":"Puente","year":"2015","journal-title":"Brain Struct. Funct."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0306-4522(99)00107-4","article-title":"Chronic administration of docosahexaenoic acid improves reference memory-related learning ability in young rats","volume":"93","author":"Gamoh","year":"1999","journal-title":"Neuroscience"},{"key":"ref_58","first-page":"3","article-title":"Alcohol\u2019s unique effects on cognition in women: A 2020 (re)view to envision future research and treatment","volume":"40","author":"Fama","year":"2020","journal-title":"Alcohol Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/12\/5507\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:48:42Z","timestamp":1760032122000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/12\/5507"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,9]]},"references-count":58,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["ijms26125507"],"URL":"https:\/\/doi.org\/10.3390\/ijms26125507","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,9]]}}}