{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T19:29:00Z","timestamp":1784230140249,"version":"3.55.0"},"reference-count":143,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:00:00Z","timestamp":1749513600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China Scholarship Council for Mengyang CAO","award":["202208080236"],"award-info":[{"award-number":["202208080236"]}]},{"name":"China Scholarship Council for Mengyang CAO","award":["KP-06-H81\/9"],"award-info":[{"award-number":["KP-06-H81\/9"]}]},{"name":"Bulgarian National Science Fund","award":["202208080236"],"award-info":[{"award-number":["202208080236"]}]},{"name":"Bulgarian National Science Fund","award":["KP-06-H81\/9"],"award-info":[{"award-number":["KP-06-H81\/9"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Ovarian tissue cryopreservation is increasingly recognized as an effective fertility preservation option for cancer patients undergoing gonadotoxic therapies. After cancer treatment, transplantation of frozen\u2013thawed ovarian tissue can restore both fertility and endocrine function. However, the threat of reintroducing malignant cells limits its application in patients with a high risk of ovarian metastasis. To eliminate potential cancer cells in grafts, a promising strategy involves isolating follicles from cryopreserved ovarian tissue and encapsulating them within biocompatible scaffolds to construct transplantable bioprosthetic ovaries. Here, we review the construction of bioprosthetic ovaries designed to mimic natural ovarian architecture, and further discuss the challenges in bioprosthetic ovary bioengineering along with potential strategies to address these issues.<\/jats:p>","DOI":"10.3390\/ijms26125545","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T09:59:14Z","timestamp":1749549554000},"page":"5545","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Construction and Bioengineering of Human Bioprosthetic Ovaries from Cryopreserved Ovarian Tissue"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5660-7074","authenticated-orcid":false,"given":"Mengyang","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, 50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3731-6116","authenticated-orcid":false,"given":"Plamen","family":"Todorov","sequence":"additional","affiliation":[{"name":"Institute of Biology and Immunology of Reproduction of Bulgarian Academy of Sciences (BAS), 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gohar","family":"Rahimi","sequence":"additional","affiliation":[{"name":"AMEDES Facharzt-fZentrum f\u00fcr Kinderwunsch, Pr\u00e4natale Medizin, Endokrinologie und Osteologie GmbH, 90518 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0282-8018","authenticated-orcid":false,"given":"Mahmoud","family":"Salama","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Biology, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1197-3399","authenticated-orcid":false,"given":"Teresa K.","family":"Woodruff","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Biology, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgenia","family":"Isachenko","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, 50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Skala","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, 50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3674-543X","authenticated-orcid":false,"given":"Volodimir","family":"Isachenko","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, 50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"The ESHRE Guideline Group on Female Fertility Preservation, Anderson, R.A., Amant, F., Braat, D., D\u2019Angelo, A., Chuva de Sousa Lopes, S.M., Demeestere, I., Dwek, S., Frith, L., and Lambertini, M. 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