{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:08:31Z","timestamp":1789564111518,"version":"build-2803163510"},"reference-count":112,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Education of the Junta de Castilla y Le\u00f3n and the European Regional Development Fund (ERDF) by TCUE Plan 2021\u20132023","award":["134\/2021"],"award-info":[{"award-number":["134\/2021"]}]},{"name":"Department of Education of the Junta de Castilla y Le\u00f3n and the European Regional Development Fund (ERDF) by TCUE Plan 2021\u20132023","award":["067\/2300007 POC"],"award-info":[{"award-number":["067\/2300007 POC"]}]},{"name":"Application of Genomics through the Study of Genetic polymorphisms in the treatment and prevention of chronic diseases in older adult patients","award":["134\/2021"],"award-info":[{"award-number":["134\/2021"]}]},{"name":"Application of Genomics through the Study of Genetic polymorphisms in the treatment and prevention of chronic diseases in older adult patients","award":["067\/2300007 POC"],"award-info":[{"award-number":["067\/2300007 POC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Vitamin D is a key micronutrient in the function of the skeletomuscular system. Athletes are at increased risk of developing vitamin D deficiency during the execution of very demanding disciplines such as CrossFit\u00ae. Single-nucleotide polymorphisms (SNPs) may influence circulating 25-hydroxy-vitamin D (25(OH)D) levels. An observational, longitudinal pilot study was conducted with 50 trained males according to specific inclusion criteria. Blood samples were obtained to determine 25(OH)D, vitamin D-binding protein (VDBP), vitamin D-receptor (VDR)circulating levels, and the presence of SNPs after DNA isolation and genotyping: rs10741657 to CYP2R1, rs2282679 to GC and rs2228570 to VDR genes. Significant differences (p &lt; 0.05) in 25(OH)D concentration were determined between the biallelic combinations of rs228679 (GC) and rs228570 (VDR). The VDBP and VDR proteins did not show different levels in the case of the rs10741657 (CYP2R1) alleles. Statistically significant weak positive correlations (p &lt; 0.05) were observed between 25(OH)D and AA-alleles of the CYP2R1 and VDR genes, and TT-alleles of the GC gene. Additionally, AA (rs10741657 and rs2228570) and TT (rs2282679) have a probability between 2 and 4 of having major effects on the concentration of 25(OH)D. Conversely, GG alleles present a probability of suboptimal values of 25(OH)D of 69%, 34%, and 24% for VDR, GC, and CYP2R1, respectively, showing a strong moderate positive correlation (r = 0.41) between the degrees of sports performance and 25(OH)D plasma levels. CYP2R1 (rs10741657), GC (rs2282679), and VDR (rs2228570) affect the concentration of serum 25(OH)D, as an indicator of vitamin D status and play a critical role in the sports performance of CrossFit\u00ae practitioners.<\/jats:p>","DOI":"10.3390\/ijms26125602","type":"journal-article","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T11:18:40Z","timestamp":1749640720000},"page":"5602","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Single-Nucleotide Polymorphisms (SNPs) in Vitamin D Physiology Genes May Modulate Serum 25(OH)D Levels in Well-Trained CrossFit\u00ae Athletes, Which May Be Associated with Performance Outcomes"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6522-8896","authenticated-orcid":false,"given":"Diego","family":"Fern\u00e1ndez-L\u00e1zaro","sequence":"first","affiliation":[{"name":"Area of Histology, Faculty of Medicine and INCYL, University of Valladolid, 47005 Valladolid, Spain"},{"name":"Neurobiology Research Group, Faculty of Medicine, University of Valladolid, 47005 Valladolid, Spain"},{"name":"Research Group \u201cNutrition and Physical Activity\u201d, Spanish Nutrition Society \u201cSE\u00d1\u201d, 28010 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6554-4602","authenticated-orcid":false,"given":"Juan","family":"Mielgo-Ayuso","sequence":"additional","affiliation":[{"name":"Research Group \u201cNutrition and Physical Activity\u201d, Spanish Nutrition Society \u201cSE\u00d1\u201d, 28010 Madrid, Spain"},{"name":"Department of Health Sciences, Faculty of Health Sciences, University of Burgos, 09001 Burgos, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7818-9777","authenticated-orcid":false,"given":"Jes\u00fas","family":"Seco-Calvo","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine (IBIOMED), University of Le\u00f3n, 27071 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2612-934X","authenticated-orcid":false,"given":"Eduardo","family":"Guti\u00e9rrez-Abej\u00f3n","sequence":"additional","affiliation":[{"name":"Pharmacological Big Data Laboratory, Department of Cell Biology, Genetics, Histology and Pharmacology, Faculty of Medicine, University of Valladolid, 47003 Valladolid, Spain"},{"name":"Pharmacy Directorate, Castilla y Leon Health Council, 47007 Valladolid, Spain"},{"name":"Atenci\u00f3n Primaria, \u00c1rea de Salud de Valladolid Este, 47010 Valladolid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5128-1672","authenticated-orcid":false,"given":"Enrique","family":"Roche","sequence":"additional","affiliation":[{"name":"Research Group \u201cNutrition and Physical Activity\u201d, Spanish Nutrition Society \u201cSE\u00d1\u201d, 28010 Madrid, Spain"},{"name":"Department of Applied Biology-Nutrition, Institute of Bioengineering, Miguel Hernandez University, 03220 Elche, Spain"},{"name":"Alicante Institute for Health and Biomedical Research (ISABIAL), 03010 Alicante, Spain"},{"name":"CIBER Fisiopatolog\u00eda de la Obesidad y Nutrici\u00f3n (CIBEROBN), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4280-5611","authenticated-orcid":false,"given":"Manuel","family":"Garrosa","sequence":"additional","affiliation":[{"name":"Area of Histology, Faculty of Medicine and INCYL, University of Valladolid, 47005 Valladolid, Spain"},{"name":"Neurobiology Research Group, Faculty of Medicine, University of Valladolid, 47005 Valladolid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1136\/bjsports-2012-091735","article-title":"The effects of vitamin D3 supplementation on serum total 25[OH]D concentration and physical performance: A randomised dose-response study","volume":"47","author":"Close","year":"2013","journal-title":"Br. 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