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Functional evidence of TRP channels\u2019 involvement in regulating cold-induced developmental\/reproductive responses remains scarce. Here, we show that mutations affecting cold-sensing TRP channels antagonize the reduction in ovarian development induced by low temperatures (reproductive dormancy) in Drosophila melanogaster. More specifically, mutants for brv1, trp, and trpl significantly lowered dormancy levels at 12 \u00b0C and exhibited well-developed oocytes characterized by advanced vitellogenesis. Similarly, functional knockouts for norpA, a gene encoding a phospholipase C acting downstream to Trp and Trpl, exhibited a reduced dormancy response, suggesting that Ca2+ signaling is key to relaying cold-sensing stimuli during dormancy induction and maintenance. Finally, mutants with an altered temperature preference (i.e., exhibiting impaired cold or warm avoidance) differentially responded to the cold, either lowering or increasing dormancy levels. In summary, our phenotypic analysis provides functional evidence of developmental\/reproductive modulation by specific cold-sensing TRP channels in Drosophila melanogaster and indicates that temperature preference affects developmental processes.<\/jats:p>","DOI":"10.3390\/ijms26125638","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T07:44:23Z","timestamp":1749714263000},"page":"5638","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cold-Sensing TRP Channels and Temperature Preference Modulate Ovarian Development in the Model Organism Drosophila melanogaster"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8857-7282","authenticated-orcid":false,"given":"Gabriele","family":"Andreatta","sequence":"first","affiliation":[{"name":"Department of Biology, University of Padua, 35131 Padua, Italy"},{"name":"Department of Comparative Biomedicine and Food Science, University of Padua, 35020 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2800-9923","authenticated-orcid":false,"given":"Sara","family":"Montagnese","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Padua, 35128 Padua, Italy"},{"name":"Chronobiology, Faculty of Health and Medical Sciences, University of Surrey, Guildford GU2 7XH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2489-9116","authenticated-orcid":false,"given":"Rodolfo","family":"Costa","sequence":"additional","affiliation":[{"name":"Chronobiology, Faculty of Health and Medical Sciences, University of Surrey, Guildford GU2 7XH, UK"},{"name":"Institute of Neuroscience, National Research Council (CNR), 35131 Padua, Italy"},{"name":"Department of Biomedical Sciences, University of Padua, 35131 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.cell.2011.01.028","article-title":"The Coding of Temperature in the Drosophila Brain","volume":"144","author":"Gallio","year":"2011","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1038\/nature10715","article-title":"Modulation of TRPA1 Thermal Sensitivity Enables Sensory Discrimination in Drosophila","volume":"481","author":"Kang","year":"2011","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1007\/s00424-006-0199-6","article-title":"Temperature Sensing across Species","volume":"454","author":"McKemy","year":"2007","journal-title":"Pflug. 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