{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T14:46:08Z","timestamp":1767969968240,"version":"3.49.0"},"reference-count":28,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T00:00:00Z","timestamp":1749686400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The upcoming wave of personalized medicine, driven by genomic diagnostics and artificial intelligence, demands clearly defined pre-laboratory and laboratory procedures to ensure the acquisition of DNA and RNA of sufficient quantity and quality. In prostate cancer oncogenetics, diagnostic and prognostic assessments increasingly rely on personalized approaches, including Comprehensive Genomic Profiling (CGP). In this pilot study, we aimed to establish optimal pre-analytical and analytical conditions for selected genetic diagnostic methods using tissue samples acquired through multiparametric MRI-guided biopsy. Tissue specimens from thirteen patients were processed for DNA isolation, fluorescence in situ hybridization (FISH), and next-generation sequencing (NGS). Comparative analyses were performed on DNA derived from both fresh and formalin-fixed, paraffin-embedded (FFPE) samples. Sequencing quality metrics demonstrated markedly superior performance in fresh tissue compared to FFPE. These results highlight the importance of standardized tissue collection and processing protocols to enable reliable molecular diagnostics in prostate cancer. Our findings support the feasibility of integrating high-quality genomic testing into routine biopsy workflows and emphasize the need for further large-scale validation.<\/jats:p>","DOI":"10.3390\/ijms26125648","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T11:47:07Z","timestamp":1749728827000},"page":"5648","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Personalized High-Resolution Genetic Diagnostics of Prostate Adenocarcinoma Guided by Multiparametric Magnetic Resonance Imaging: Results of a Pilot Study"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4243-220X","authenticated-orcid":false,"given":"Jacek","family":"Wilkosz","sequence":"first","affiliation":[{"name":"Second Department of Urology, Medical University of Lodz, 93-513 Lodz, Poland"},{"name":"Department of General, Oncological, and Functional Urology, Copernicus Memorial Voivodship Multidisciplinary Centre for Oncology and Traumatology, Medical University of Lodz, 93-513 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dariusz Wojciech","family":"Sobieraj","sequence":"additional","affiliation":[{"name":"Department of Urology and Urological Oncology, John Paul II Podkarpackie Province Hospital in Krosno, 38-400 Krosno, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadeusz","family":"Ka\u0142u\u017cewski","sequence":"additional","affiliation":[{"name":"Department of Genetics, Polish Mother\u2019s Memorial Hospital Research Institute, 93-338 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jakub","family":"Kaczmarek","sequence":"additional","affiliation":[{"name":"General Surgery Department\u2014Endoscopy Unit and One-Day Surgery Department (Urology), Bonifraters\u2019 Medical Center, 93-378 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaros\u0142aw","family":"Szwalski","sequence":"additional","affiliation":[{"name":"CYTOPATH S.A., Histopathological Laboratory, 90-552 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micha\u0142","family":"Bednarek","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Genetics, R&D Division, GENOS Sp. z o.o., 91-033 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Morel","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Genetics, R&D Division, GENOS Sp. z o.o., 91-033 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u017baneta","family":"Kasprzyk","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Genetics, R&D Division, GENOS Sp. z o.o., 91-033 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2882-5448","authenticated-orcid":false,"given":"\u0141ukasz","family":"K\u0119pczy\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Genetics, Polish Mother\u2019s Memorial Hospital Research Institute, 93-338 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordan","family":"Sa\u0142amunia","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Genetics, R&D Division, GENOS Sp. z o.o., 91-033 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Gach","sequence":"additional","affiliation":[{"name":"Department of Genetics, Polish Mother\u2019s Memorial Hospital Research Institute, 93-338 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bogdan","family":"Ka\u0142u\u017cewski","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Genetics, R&D Division, GENOS Sp. z o.o., 91-033 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.5603\/njo.99065","article-title":"Cancer Incidence and Mortality in Poland in 2023","volume":"74","author":"Didkowska","year":"2024","journal-title":"Nowotw. 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