{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:23:43Z","timestamp":1760059423502,"version":"build-2065373602"},"reference-count":31,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T00:00:00Z","timestamp":1749686400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32171238","82173466","82473579"],"award-info":[{"award-number":["32171238","82173466","82473579"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>With growing public concern about the health effects of low-dose radiation, numerous studies have demonstrated that low-dose radiation can cause damage to the immune system, making intervention measures essential. This study investigated the protective effects of silybin against low-dose radiation-induced immune system damage and its underlying mechanisms at both the cellular and animal levels. At the cellular level, CCK-8 assays, ROS measurements, and RT-qPCR analysis revealed that silybin alleviated the reduction in RAW264.7 cell proliferation, intracellular ROS levels, and inflammatory cytokine expression following low-dose radiation exposure. At the animal level, comparative analyses of post-irradiation body weight, peripheral blood cell counts, immune organ coefficients, spleen HE\/IHC staining, and spleen immune cell numbers demonstrated that silybin mitigated the radiation-induced decrease in body weight, reduction in peripheral blood leukocyte counts, inflammatory cell infiltration in the spleen, decline in spleen immune cell numbers, and increase in cGAS protein-positive cells. These findings indicate that silybin exerts protective effects against low-dose radiation-induced immune system damage, potentially by regulating the cGAS signaling pathway to reduce radiation-induced cellular injury, thereby enhancing its radioprotective properties.<\/jats:p>","DOI":"10.3390\/ijms26125656","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T03:53:12Z","timestamp":1749786792000},"page":"5656","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Studies on the Protective Effect of Silybin Against Low-Dose Radiation-Induced Damage to the Immune System"],"prefix":"10.3390","volume":"26","author":[{"given":"Yu","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Public Health, University of South China, Hengyang 421001, China"},{"name":"Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanan","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Public Health, University of South China, Hengyang 421001, China"},{"name":"Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Public Health, University of South China, Hengyang 421001, China"},{"name":"Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lanfang","family":"Ma","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China"},{"name":"School of Life Science, Hebei University, Baoding 071000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Xu","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lehan","family":"Ding","sequence":"additional","affiliation":[{"name":"School of Public Health, University of South China, Hengyang 421001, China"},{"name":"Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongling","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weixiang","family":"Hu","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7660-0393","authenticated-orcid":false,"given":"Kai","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping-Kun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Guan","sequence":"additional","affiliation":[{"name":"School of Public Health, University of South China, Hengyang 421001, China"},{"name":"Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China"},{"name":"School of Life Science, Hebei University, Baoding 071000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.mrfmmm.2010.01.007","article-title":"Non-cancer diseases and non-targeted effects","volume":"687","author":"Hildebrandt","year":"2010","journal-title":"Mutat. 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