{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T19:09:10Z","timestamp":1778180950532,"version":"3.51.4"},"reference-count":84,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T00:00:00Z","timestamp":1749772800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Laboratory of Molecular Nutrition and Proteomics, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Romania"},{"name":"\u201cEugen Ionescu\u201d doctoral and postdoctoral research scholarships, UAF"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Arthrospira platensis, a filamentous photosynthetic cyanobacterium, is widely recognized for its high nutritional value, broad spectrum of bioactive compounds, and excellent safety profile, making it a promising natural source for health-promoting applications. This study aimed to profile the phenolic constituents of an ethanolic extract of A. platensis (EAP) using HPLC-DAD-ESI-MS and to investigate its pharmacological effects in attenuating acute and sub-chronic experimental colitis, as well as its antioxidant and antifungal properties. Colitis was induced in BALB\/c mice by intrarectal administration of 2,4-dinitrobenzenesulfonic acid (DNBS), followed by oral administration of EAP at doses of 50, 100, and 200 mg\/kg. Phenolic profiling revealed eight major compounds, with a cumulative content of 6.777 mg\/g of extract, with Pyrogallol, Ferulic acid, and Chlorogenic acid being the most abundant. In vivo, EAP treatment significantly reduced the Disease Activity Index (DAI), alleviated macroscopic colonic damage, and preserved colonic mucosal integrity in both inflammatory phases. Biochemical analyses revealed significant reductions in myeloperoxidase (MPO) activity, nitric oxide (NO), and malondialdehyde (MDA) levels, accompanied by increased reduced glutathione (GSH) content and catalase activity. In vitro, EAP demonstrated notable antioxidant effects, including 56% DPPH and 47% ABTS radical scavenging activities, and an 81% ferrous ion-chelating capacity. Furthermore, it exhibited antifungal activity, with inhibition zones of 20 mm against Candida albicans and 15 mm against Aspergillus flavus, respectively. These findings highlight the multitarget bioactivity of EAP and support its potential as a natural agent for managing intestinal inflammation and oxidative stress across both acute and sub-chronic phases.<\/jats:p>","DOI":"10.3390\/ijms26125692","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T09:51:24Z","timestamp":1749808284000},"page":"5692","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Phenolic Profiling and Bioactive Properties of Arthrospira platensis Extract in Alleviating Acute and Sub-Chronic Colitis"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4971-8189","authenticated-orcid":false,"given":"Meriem","family":"Aziez","sequence":"first","affiliation":[{"name":"Laboratory of Plant Biotechnology and Ethnobotany, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia 06000, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8306-8670","authenticated-orcid":false,"given":"Ramona","family":"Suharoschi","sequence":"additional","affiliation":[{"name":"Molecular Nutrition and Proteomics Lab, Bld. Life Science Institute, Department of Food Science, University of Agricultural Science and Veterinary Medicine, 3-5 Calea M\u0103n\u0103stur, 400372 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed Sofiane","family":"Merakeb","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Biotechnology and Ethnobotany, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia 06000, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8939-7267","authenticated-orcid":false,"given":"Oana Lelia","family":"Pop","sequence":"additional","affiliation":[{"name":"Molecular Nutrition and Proteomics Lab, Bld. Life Science Institute, Department of Food Science, University of Agricultural Science and Veterinary Medicine, 3-5 Calea M\u0103n\u0103stur, 400372 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5284-0683","authenticated-orcid":false,"given":"C\u0103lina","family":"Ciont","sequence":"additional","affiliation":[{"name":"Molecular Nutrition and Proteomics Lab, Bld. Life Science Institute, Department of Food Science, University of Agricultural Science and Veterinary Medicine, 3-5 Calea M\u0103n\u0103stur, 400372 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Floricu\u021ba","family":"Ranga","sequence":"additional","affiliation":[{"name":"Food Biotechnology and Molecular Gastronomy, Bld. 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Life Science Institute, Department of Food Science, University of Agricultural Science and Veterinary Medicine, Calea M\u0103n\u0103stur 3-5, 400372 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Cozma","sequence":"additional","affiliation":[{"name":"Clinical Center of Diabetes, Nutrition and Metabolic Diseases, Cluj-Napoca, Faculty of Medicine, \u201cIuliu-Ha\u021bieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1124-6984","authenticated-orcid":false,"given":"Adriana","family":"Fodor","sequence":"additional","affiliation":[{"name":"Internal Medicine Department, 4th Medical Clinic \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elhadia","family":"Mansouri","sequence":"additional","affiliation":[{"name":"Laboratory of Toxicology, Faculty of Pharmacy of Algiers, Algiers 16000, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dalila","family":"Smati","sequence":"additional","affiliation":[{"name":"Pharmacy Department, Faculty of Medicine, Benyoucef Benkhadda University, Alger Centre 16000, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noureddine","family":"Bribi","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Biotechnology and Ethnobotany, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia 06000, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1146\/annurev-immunol-030409-101225","article-title":"Inflammatory Bowel Disease","volume":"28","author":"Kaser","year":"2010","journal-title":"Annu. 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