{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T14:43:29Z","timestamp":1780497809161,"version":"3.54.1"},"reference-count":87,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,14]],"date-time":"2025-06-14T00:00:00Z","timestamp":1749859200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"SNUBH Research Fund","award":["02-2021-0009"],"award-info":[{"award-number":["02-2021-0009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Wound healing remains a significant clinical challenge worldwide, and effective management strategies are essential for improving outcomes. This study evaluated SCderm Matrix, a novel acellular dermal matrix (ADM) patch developed using supercritical carbon dioxide (sCO2) processing of human skin tissue. This innovative processing method preserves structural integrity while enhancing biocompatibility, resulting in a patch characterized by porous architecture, uniform thickness, excellent tensile strength, and optical transparency. In vivo wound healing experiments using full-thickness skin wounds in Sprague\u2013Dawley rats demonstrated the patch\u2019s superior performance. Treatment with the sCO2 ADM patch accelerated wound closure, reduced inflammation, and enhanced granulation tissue formation compared to both untreated controls and two commercially available ADM products. Histological analysis revealed improved re-epithelialization and collagen deposition, while molecular and immunohistochemical assessments showed decreased reactive oxygen species (ROS) and pro-inflammatory cytokines. Simultaneously, the treatment upregulated key proliferation and remodeling markers including alpha smooth muscle actin (\u03b1-SMA), vimentin, and transforming growth factor beta 1 (TGF-\u03b21). These findings demonstrate that the SCderm Matrix promotes wound healing through multiple mechanisms: modulating inflammatory responses, enhancing antioxidant defenses, and supporting tissue regeneration. The results suggest this biomaterial has significant potential as an effective and versatile solution for clinical wound care applications.<\/jats:p>","DOI":"10.3390\/ijms26125715","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T09:51:22Z","timestamp":1750067482000},"page":"5715","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Supercritical Carbon Dioxide-Processed Acellular Dermal Matrix Patch for Enhanced Wound Healing"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5615-6341","authenticated-orcid":false,"given":"Xinrui","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"},{"name":"Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7193-9053","authenticated-orcid":false,"given":"Linh Thi Thuy","family":"Le","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea"},{"name":"Department of Biomedical Science, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"},{"name":"Department of Medical Technology, Haiphong University of Medicine and Pharmacy, Haiphong 180000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxun","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"},{"name":"Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caijun","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"},{"name":"Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Ngan","family":"Giang","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea"},{"name":"Department of Medical Device Development, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4541-8198","authenticated-orcid":false,"given":"Thuy-Tien Thi","family":"Trinh","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea"},{"name":"Korean Institute of Nonclinical Study (KINS), Seongnam 13605, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Korean Institute of Nonclinical Study (KINS), Seongnam 13605, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong Woo","family":"Shin","sequence":"additional","affiliation":[{"name":"DOF Inc., Hwaseong 18468, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Woo","family":"Bae","sequence":"additional","affiliation":[{"name":"DOF Inc., Hwaseong 18468, Republic of 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