{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T23:12:18Z","timestamp":1768432338999,"version":"3.49.0"},"reference-count":56,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,15]],"date-time":"2025-06-15T00:00:00Z","timestamp":1749945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The use of plastic products has increased, leading to higher levels of plastic pollution, and it is becoming a major public health concern. Health risks\u2014especially those related to reproductive system dysfunction caused by polystyrene microplastics (PS-MPs)\u2014are emerging issues that require urgent attention. This study aimed to investigate the effects of erinacine A-enriched Hericium erinaceus mycelium (HE) on high-fat-diet- and PS-MP-induced reproductive system dysfunction in male rats. Reproductive dysfunction was induced by administering a high-fat diet followed by exposure to PS-MPs for six weeks. The results showed that HE treatment significantly reduced nitric oxide levels and enhanced glutathione peroxidase activity. Furthermore, HE supplementation significantly downregulated pro-inflammatory cytokines such as interleukin (IL)-6 and IL-1\u03b2. Additionally, HE treatment significantly increased Kiss1 concentration, upregulated follicle-stimulating hormone and testosterone levels, reduced the area of the seminiferous tubule lumen, and prevented a reduction in epithelial thickness. HE treatment also significantly increased sperm count and reduced sperm abnormalities. Based on these findings, HE supplementation helps prevent reproductive system dysfunction by reducing oxidative stress and pro-inflammatory cytokines. Therefore, erinacine A-enriched H. erinaceus mycelium could be considered a potential food supplement or functional food ingredient for the treatment of reproductive or testicular dysfunction.<\/jats:p>","DOI":"10.3390\/ijms26125735","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T10:47:22Z","timestamp":1750070842000},"page":"5735","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Yamabushitake Mushroom (Hericium erinaceus (Bull.) Pers. 1797) Mycelium Improves Reproductive System Dysfunction in Male Rats Induced by Polystyrene Microplastics"],"prefix":"10.3390","volume":"26","author":[{"given":"Yi-Yuh","family":"Hwang","sequence":"first","affiliation":[{"name":"Department of Food Science, National Taiwan Ocean University, Keelung 20224, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2821-3772","authenticated-orcid":false,"given":"Sabri","family":"Sudirman","sequence":"additional","affiliation":[{"name":"Department of Fisheries Product Technology, Faculty of Agriculture, Universitas Sriwijaya, Indralaya 30662, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"En-Yu","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Food Science, National Taiwan Ocean University, Keelung 20224, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4020-0600","authenticated-orcid":false,"given":"Ruei-Feng","family":"Shiu","sequence":"additional","affiliation":[{"name":"Institute of Marine Environment and Ecology, National Taiwan Ocean University, Keelung 20224, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4877-6524","authenticated-orcid":false,"given":"Zwe-Ling","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Food Science, National Taiwan Ocean University, Keelung 20224, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deng-Fwu","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Food Science, National Taiwan Ocean University, Keelung 20224, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ghosh, S., Sinha, J.K., Ghosh, S., Vashisth, K., Han, S., and Bhaskar, R. (2023). Microplastics as an emerging threat to the global environment and human health. Sustainability, 15.","DOI":"10.3390\/su151410821"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Wang, W., Guan, J., Feng, Y., Nie, L., Xu, Y., Xu, H., and Fu, F. (2023). Polystyrene microplastics induced nephrotoxicity associated with oxidative stress, inflammation, and endoplasmic reticulum stress in juvenile rats. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.1059660"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"159834","DOI":"10.1016\/j.scitotenv.2022.159834","article-title":"Microplastics in human food chains: Food becoming a threat to health safety","volume":"858","author":"Mamun","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"173177","DOI":"10.1016\/j.scitotenv.2024.173177","article-title":"The hidden threat: Unraveling the impact of microplastics on reproductive health","volume":"935","author":"Wang","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Dubey, I., Khan, S., and Kushwaha, S. (2022). Developmental and reproductive toxic effects of exposure to microplastics: A review of associated signaling pathways. Front. Toxicol., 4.","DOI":"10.3389\/ftox.2022.901798"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Ijaz, M.U., Najam, S., Hamza, A., Azmat, R., Ashraf, A., Unuofin, J.O., Lebelo, S.L., and Simal-Gandara, J. (2023). Pinostrobin alleviates testicular and spermatological damage induced by polystyrene microplastics in adult albino rats. Biomed. Pharmacother., 162.","DOI":"10.1016\/j.biopha.2023.114686"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"124028","DOI":"10.1016\/j.jhazmat.2020.124028","article-title":"Reproductive toxicity of polystyrene microplastics: In vivo experimental study on testicular toxicity in mice","volume":"405","author":"Hou","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Hwang, Y.-Y., Sudirman, S., Wei, E.-Y., Kong, Z.-L., and Hwang, D.-F. (2024). Fucoidan from cladosiphon okamuranus enhances antioxidant activity and prevents reproductive dysfunction in polystyrene microplastic-induced male rats. Biomed. Pharmacother., 170.","DOI":"10.1016\/j.biopha.2023.115912"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"15579883221096549","DOI":"10.1177\/15579883221096549","article-title":"Microplastics may be a significant cause of male infertility","volume":"16","author":"Zhang","year":"2022","journal-title":"Am. J. Men\u2019s Health"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s12989-022-00453-2","article-title":"Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the lh-mediated lhr\/camp\/pka\/star pathway","volume":"19","author":"Jin","year":"2022","journal-title":"Part. Fibre Toxicol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"116180","DOI":"10.1016\/j.ecoenv.2024.116180","article-title":"Combined effects of polystyrene nanoplastics and lipopolysaccharide on testosterone biosynthesis and inflammation in mouse testis","volume":"273","author":"Li","year":"2024","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"115248","DOI":"10.1016\/j.ecoenv.2023.115248","article-title":"Probiotics improve polystyrene microplastics-induced male reproductive toxicity in mice by alleviating inflammatory response","volume":"263","author":"Zhang","year":"2023","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"110133","DOI":"10.1016\/j.ecoenv.2019.110133","article-title":"Exposure to polystyrene microplastics causes reproductive toxicity through oxidative stress and activation of the p38 mapk signaling pathway","volume":"190","author":"Xie","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e24013","DOI":"10.53388\/IMD202408013","article-title":"Hericium erinaceus: The enchanting medicinal-culinary mushroom of east asian tradition","volume":"8","author":"Qi","year":"2024","journal-title":"Integr. Med. Discov."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Chutimanukul, P., Phatthanamas, W., Thepsilvisut, O., Chantarachot, T., Thongtip, A., and Chutimanukul, P. (2023). Commercial scale production of yamabushitake mushroom (hericium erinaceus (bull.) pers. 1797) using rubber and bamboo sawdust substrates in tropical regions. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-40601-y"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3048","DOI":"10.3748\/wjg.v29.i20.3048","article-title":"Hericium erinaceus, a medicinal fungus with a centuries-old history: Evidence in gastrointestinal diseases","volume":"29","author":"Gravina","year":"2023","journal-title":"World J. Gastroenterol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Szu\u0107ko-Kociuba, I., Trzeciak-Ryczek, A., Kupnicka, P., and Chlubek, D. (2023). Neurotrophic and neuroprotective effects of hericium erinaceus. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242115960"},{"key":"ref_18","first-page":"676","article-title":"The chemical structures, biosynthesis, and biological activities of secondary metabolites from the culinary-medicinal mushrooms of the genus hericium: A review","volume":"22","author":"Qi","year":"2024","journal-title":"Chin. J. Nat. Med."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wu, Y.-L., Sun, H.-L., Chang, J.-C., Lin, W.-Y., Chen, P.-Y., Chen, C.-C., Lee, L.-Y., Li, C.-C., Hsieh, M., and Chen, H.-W. (2024). Erinacine a-enriched hericium erinaceus mycelium ethanol extract lessens cellular damage in cell and drosophila models of spinocerebellar ataxia type 3 by improvement of nrf2 activation. Antioxidants, 13.","DOI":"10.3390\/antiox13121495"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wang, L.-Y., Huang, C.-S., Chen, Y.-H., Chen, C.-C., Chen, C.-C., and Chuang, C.-H. (2019). Anti-inflammatory effect of erinacine c on no production through down-regulation of nf-\u03bab and activation of nrf2-mediated ho-1 in bv2 microglial cells treated with lps. Molecules, 24.","DOI":"10.3390\/molecules24183317"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"15073","DOI":"10.3390\/ijms150915073","article-title":"Protective effects of hericium erinaceus mycelium and its isolated erinacine a against ischemia-injury-induced neuronal cell death via the inhibition of inos\/p38 mapk and nitrotyrosine","volume":"15","author":"Lee","year":"2014","journal-title":"Int. J. Mol. Sci."},{"key":"ref_22","first-page":"e030624230635","article-title":"Metabolites of culinary-medicinal mushroom hericium erinaceus showed anti-neuroinflammatory activity in bv2 cells and anti-h2o2-induced oxidative stress activity in sh-sy5y cells","volume":"15","author":"Wei","year":"2025","journal-title":"Nat. Prod. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.26599\/FSHW.2022.9250152","article-title":"Bioactive compounds in hericium erinaceus and their biological properties: A review","volume":"13","author":"Qiu","year":"2024","journal-title":"Food Sci. Hum. Wellness"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/s41429-018-0065-8","article-title":"Three new cyathane diterpenes with neurotrophic activity from the liquid cultures of hericium erinaceus","volume":"71","author":"Zhang","year":"2018","journal-title":"J. Antibiot."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e37850","DOI":"10.1016\/j.heliyon.2024.e37850","article-title":"A pilot pharmacokinetic and metabolite identification study of erinacine a in a single landrace pig model","volume":"10","author":"Chen","year":"2024","journal-title":"Heliyon"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.1089\/ars.2010.3586","article-title":"Glutathione peroxidase-1 in health and disease: From molecular mechanisms to therapeutic opportunities","volume":"15","author":"Lubos","year":"2011","journal-title":"Antioxid. Redox Signal."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"104010","DOI":"10.1016\/j.mvr.2020.104010","article-title":"The role of glutathione and glutathione peroxidase in regulating cellular level of reactive oxygen and nitrogen species","volume":"131","author":"Panday","year":"2020","journal-title":"Microvasc. Res."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Lee, L.-Y., Chou, W., Chen, W.-P., Wang, M.-F., Chen, Y.-J., Chen, C.-C., and Tung, K.-C. (2021). Erinacine a-enriched hericium erinaceus mycelium delays progression of age-related cognitive decline in senescence accelerated mouse prone 8 (samp8) mice. Nutrients, 13.","DOI":"10.3390\/nu13103659"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Chiu, C.-H., Chyau, C.-C., Chen, C.-C., Lee, L.-Y., Chen, W.-P., Liu, J.-L., Lin, W.-H., and Mong, M.-C. (2018). Erinacine a-enriched hericium erinaceus mycelium produces antidepressant-like effects through modulating bdnf\/pi3k\/akt\/gsk-3\u03b2 signaling in mice. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19020341"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Lee, S.-L., Hsu, J.-Y., Chen, T.-C., Huang, C.-C., Wu, T.-Y., and Chin, T.-Y. (2022). Erinacine a prevents lipopolysaccharide-mediated glial cell activation to protect dopaminergic neurons against inflammatory factor-induced cell death in vitro and in vivo. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23020810"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Ogawa, S., and Parhar, I.S. (2018). Biological significance of kisspeptin\u2013kiss 1 receptor signaling in the habenula of teleost species. Front. Endocrinol., 9.","DOI":"10.3389\/fendo.2018.00222"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1093\/humupd\/dmu009","article-title":"The kisspeptin-gnrh pathway in human reproductive health and disease","volume":"20","author":"Skorupskaite","year":"2014","journal-title":"Hum. Reprod. Update"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1016\/j.fertnstert.2019.01.034","article-title":"Association between testosterone, semen parameters, and live birth in men with unexplained infertility in an intrauterine insemination population","volume":"111","author":"Trussell","year":"2019","journal-title":"Fertil. Steril."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"309","DOI":"10.4103\/aja.aja_42_19","article-title":"Fsh dosage effect on conventional sperm parameters: A meta-analysis of randomized controlled studies","volume":"22","author":"Cannarella","year":"2020","journal-title":"Asian J. Androl."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Grande, G., Barrachina, F., Soler-Ventura, A., Jodar, M., Mancini, F., Marana, R., Chiloiro, S., Pontecorvi, A., Oliva, R., and Milardi, D. (2022). The role of testosterone in spermatogenesis: Lessons from proteome profiling of human spermatozoa in testosterone deficiency. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.852661"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Guardo, F.D., Vloeberghs, V., Bardhi, E., Blockeel, C., Verheyen, G., Tournaye, H., and Drakopoulos, P. (2020). Low testosterone and semen parameters in male partners of infertile couples undergoing ivf with a total sperm count greater than 5 million. J. Clin. Med., 9.","DOI":"10.3390\/jcm9123824"},{"key":"ref_37","first-page":"123","article-title":"Impact of inflammation on male reproductive tract","volume":"16","author":"Azenabor","year":"2015","journal-title":"J. Reprod. Infertil."},{"key":"ref_38","first-page":"e42583","article-title":"The impact of oxidative stress on male reproductive function: Exploring the role of antioxidant supplementation","volume":"17","author":"Walke","year":"2023","journal-title":"Cureus"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"60","DOI":"10.5173\/ceju.2013.01.art19","article-title":"The role of oxidative stress and antioxidants in male fertility","volume":"65","author":"Wolski","year":"2013","journal-title":"Cent. Eur. J. Urol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1515\/jcim-2013-0001","article-title":"Hericium erinaceus: An edible mushroom with medicinal values","volume":"10","author":"Khan","year":"2013","journal-title":"J. Complement. Integr. Med."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Hasan, H., Bhushan, S., Fijak, M., and Meinhardt, A. (2022). Mechanism of inflammatory associated impairment of sperm function, spermatogenesis and steroidogenesis. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.897029"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"236","DOI":"10.5115\/acb.22.229","article-title":"Co-administration of alcohol and combination antiretroviral therapy (cart) in male sprague dawley rats: A study on testicular morphology, oxidative and cytokines perturbations","volume":"56","author":"Owembabazi","year":"2023","journal-title":"Anat. Cell Biol."},{"key":"ref_43","first-page":"e13615","article-title":"How defective spermatogenesis affects sperm DNA integrity","volume":"53","author":"Kuchakulla","year":"2020","journal-title":"Andrologia"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/j.toxrep.2023.04.006","article-title":"Apoptosis, inflammation, and oxidative stress in infertility: A mini review","volume":"10","author":"Ojo","year":"2023","journal-title":"Toxicol. Rep."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"170958","DOI":"10.1016\/j.peptides.2023.170958","article-title":"The impact of inflammatory stress on hypothalamic kisspeptin neurons: Mechanisms underlying inflammation-associated infertility in humans and domestic animals","volume":"162","author":"Magata","year":"2023","journal-title":"Peptides"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e17023","DOI":"10.1038\/sigtrans.2017.23","article-title":"Nf-\u03bab signaling in inflammation","volume":"2","author":"Liu","year":"2017","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Tsai, P.-C., Wu, Y.-K., Hu, J.-H., Li, I.C., Lin, T.-W., Chen, C.-C., and Kuo, C.-F. (2021). Preclinical bioavailability, tissue distribution, and protein binding studies of erinacine a, a bioactive compound from hericium erinaceus mycelia using validated lc-ms\/ms method. Molecules, 26.","DOI":"10.3390\/molecules26154510"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Tsai-Teng, T., Chin-Chu, C., Li-Ya, L., Wan-Ping, C., Chung-Kuang, L., Chien-Chang, S., Chi-Ying, H.F., Chien-Chih, C., and Shiao, Y.-J. (2016). Erinacine a-enriched hericium erinaceus mycelium ameliorates alzheimer\u2019s disease-related pathologies in appswe\/ps1de9 transgenic mice. J. Biomed. Sci., 23.","DOI":"10.1186\/s12929-016-0266-z"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"141469","DOI":"10.1016\/j.scitotenv.2020.141469","article-title":"Nano- and microplastics trigger secretion of protein-rich extracellular polymeric substances from phytoplankton","volume":"748","author":"Shiu","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"100068","DOI":"10.1016\/j.crtox.2022.100068","article-title":"Subchronic toxicity and genotoxicity studies of hericium erinaceus \u03b2-glucan extract preparation","volume":"3","author":"Chen","year":"2022","journal-title":"Curr. Res. Toxicol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.fct.2014.04.040","article-title":"Evaluation of the toxicological safety of erinacine a-enriched hericium erinaceus in a 28-day oral feeding study in sprague\u2013dawley rats","volume":"70","author":"Li","year":"2014","journal-title":"Food Chem. Toxicol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Sudirman, S., Su, C.-Y., Tsou, D., Lee, M.-C., and Kong, Z.-L. (2021). Hippocampus kuda protein hydrolysate improves male reproductive dysfunction in diabetic rats. Biomed. Pharmacother., 140.","DOI":"10.1016\/j.biopha.2021.111760"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Mussbacher, M., Schrottmaier, W.C., Salzmann, M., Brostjan, C., Schmid, J.A., Starlinger, P., and Assinger, A. (2017). Optimized plasma preparation is essential to monitor platelet-stored molecules in humans. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0188921"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Baek, S.-H., Park, T., Kang, M.-G., and Park, D. (2020). Anti-inflammatory activity and ros regulation effect of sinapaldehyde in lps-stimulated raw 264.7 macrophages. Molecules, 25.","DOI":"10.3390\/molecules25184089"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.reprotox.2010.11.010","article-title":"Effect of trans-resveratrol on induced oxidative stress in human sperm and in rat germinal cells","volume":"31","author":"Collodel","year":"2011","journal-title":"Reprod. Toxicol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1293\/tox.2019-0072","article-title":"Effects of 4% paraformaldehyde and modified davidson\u2019s fluid on the morphology and immunohistochemistry of xiang pig testes","volume":"33","author":"Wang","year":"2020","journal-title":"J. Toxicol. Pathol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/12\/5735\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:52:21Z","timestamp":1760032341000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/12\/5735"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,15]]},"references-count":56,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["ijms26125735"],"URL":"https:\/\/doi.org\/10.3390\/ijms26125735","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,15]]}}}