{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:00:46Z","timestamp":1791644446704,"version":"4.3.4"},"reference-count":57,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,15]],"date-time":"2025-06-15T00:00:00Z","timestamp":1749945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study explored the extraction of genistein-7-O-[\u03b1-rhamnopyranosyl-(1\u21926)]-\u03b2-glucopyranoside (GTG) from Derris scandens using an aqueous-ethanol solvent system, aiming to optimize yield and antioxidant activity. Hansen solubility parameters (HSP) were employed to determine the optimal solvent composition, with the highest GTG yield (6.83 \u00b1 0.06 mg\/g dried weight) obtained from 50% ethanol\u2014correlating well with HSP predictions. Ultrasonic extraction was most effective with solvents having a dielectric constant between 50 and 60. The antioxidant potential of isolated GTG was evaluated using the DPPH assay, which yielded an IC50 of 87.86 \u00b1 1.85 \u03bcM, and the FRAP assay, with a value of 34.23 \u00b1 2.75 mg FeSO4 equivalents. Molecular orbital analysis revealed HOMO and LUMO energy gaps (\u0394E = 10.6715 eV) similar to known antioxidants such as gallic acid, ascorbic acid, Trolox, and quercetin. These findings demonstrate that HSP effectively guided solvent selection for ultrasound-assisted extraction of GTG. The antioxidant activity is attributed to GTG\u2019s capacity to donate electrons and stabilize radicals via extended charge delocalization within the aglycone structure, confirming its potential as a natural antioxidant agent.<\/jats:p>","DOI":"10.3390\/ijms26125740","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T10:47:22Z","timestamp":1750070842000},"page":"5740","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Application of Hansen Solubility Parameters in the Aqueous-Ethanol Extraction of Genistein-7-O-[\u03b1-rhamnopyranosyl-(1\u21926)]-\u03b2-glucopyranoside from Derris scandens and Its Molecular Orbital Study on Antioxidant Activity"],"prefix":"10.3390","volume":"26","author":[{"given":"Thitiporn","family":"Tantinithiphong","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok 26120, Thailand"},{"name":"Technical and Planning Division, Department of Thai Traditional and Alternative Medicine, Ministry of Public Health, Nonthaburi 11000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanna","family":"Eiamart","sequence":"additional","affiliation":[{"name":"Chula Pharmacokinetic Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarin","family":"Tadtong","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok 26120, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suwanna","family":"Vorarat","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok 26120, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9301-5038","authenticated-orcid":false,"given":"Weerasak","family":"Samee","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok 26120, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1055\/s-2004-827147","article-title":"Anti-inflammatory isoflavonoids from the stems of Derris scandens","volume":"70","author":"Laupattarakasem","year":"2004","journal-title":"Planta Medica"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"227","DOI":"10.3329\/bjb.v53i2.74032","article-title":"Chemical and Biological Profiling of Derris scandens (Roxb.) 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