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In this work, we aimed to further characterize the PBE cell line as a reliable in vitro model for investigating swine influenza virus (SIV) infection and immunity. We evaluated the capacity of two SIV subtypes, H1N1 and H3N2, to replicate and induce cytopathic effects in PBE cells and to modulate the expressions of IFNs, antiviral factors, inflammatory cytokines, and negative regulators of the TLR signaling. We demonstrated that PBE cells are susceptible to both H1N1 and H3N2. SIV infected PBE cells inducing notable cytopathic effects as shown by the alteration of transepithelial electrical resistance (TEER) and cilia. Both SIV subtypes replicated in PBE cells in similar proportion and altered TEER values in comparable magnitudes. However, SIV H3N2 induced higher alterations of cilia than H1N1. SIV infection induced changes in all the immune factors evaluated in PBE cells. We detected quantitative differences when the subtypes H1N1 and H3N2 were compared. The fold expression changes of IFN-\u03b2, Mx1, Mx2, IFITM1, OAS1, OAS2, and OASL were higher in PBE cells infected with H3N2 than in cells challenged with H1N1. In addition, although both subtypes stimulated IL-8 expression, only the H3N2 induced IL-6 in infected PBE cells. SIV H1N1 and H3N2 also upregulated the expressions of the negative regulators A20, BCL-3, and MKP-1, while only H1N1 increased SIGIRR and Tollip. Immortalized respiratory cell lines from pigs can be useful in vitro systems for the study of viral infections and immune responses. These studies are of importance in the context of influenza infections not only for the agricultural field because pigs are natural hosts of these viruses but also because these animals serve as intermediate reservoirs of viruses that can threaten humans\u2019 health. We demonstrated here that the PBE cell line can be a useful in vitro model to study SIV infection and immunity.<\/jats:p>","DOI":"10.3390\/ijms26125764","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T10:47:22Z","timestamp":1750070842000},"page":"5764","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Originally Established PBE Cell Line as a Reliable In Vitro Model for Investigating SIV Infection and Immunity"],"prefix":"10.3390","volume":"26","author":[{"given":"Xi-Chen","family":"Bai","sequence":"first","affiliation":[{"name":"Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kohtaro","family":"Fukuyama","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8216-2190","authenticated-orcid":false,"given":"Leonardo","family":"Albarracin","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"},{"name":"Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiya","family":"Imamura","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fu","family":"Namai","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"},{"name":"Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weichen","family":"Gong","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"},{"name":"Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wakako","family":"Ikeda-Ohtsubo","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"},{"name":"Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keita","family":"Nishiyama","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"},{"name":"Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4941-4960","authenticated-orcid":false,"given":"Julio","family":"Villena","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"},{"name":"Division of Animal Immunology and Omics, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4586-5553","authenticated-orcid":false,"given":"Haruki","family":"Kitazawa","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"},{"name":"Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.molimm.2013.02.008","article-title":"Review: Influenza virus in pigs","volume":"55","author":"Crisci","year":"2013","journal-title":"Mol. Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Sarli, G., D\u2019Annunzio, G., Gobbo, F., Benazzi, C., and Ostanello, F. (2021). The role of pathology in the diagnosis of swine respiratory disease. Vet. Sci., 8.","DOI":"10.3390\/vetsci8110256"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1038\/s41579-021-00639-z","article-title":"Infectious disease in an era of global change","volume":"20","author":"Baker","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"198118","DOI":"10.1016\/j.virusres.2020.198118","article-title":"Swine influenza virus: Current status and challenge","volume":"288","author":"Ma","year":"2020","journal-title":"Virus Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e2320303121","DOI":"10.1073\/pnas.2320303121","article-title":"Tropism for ciliated cells is the dominant driver of influenza viral burst size in the human airway","volume":"121","author":"Roach","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Punyadarsaniya, D., Liang, C.H., Winter, C., Petersen, H., Rautenschlein, S., Hennig-Pauka, I., Schwegmann-Wessels, C., Wu, C.Y., Wong, C.H., and Herrler, G. (2011). Infection of differentiated porcine airway epithelial cells by influenza virus: Differential susceptibility to infection by porcine and avian viruses. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0028429"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1016\/S2213-2600(18)30236-4","article-title":"Tropism, replication competence, and innate immune responses of influenza virus: An analysis of human airway organoids and ex-vivo bronchus cultures","volume":"6","author":"Hui","year":"2018","journal-title":"Lancet Respir. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"7469","DOI":"10.1128\/JVI.02677-05","article-title":"Influenza virus receptor specificity and cell tropism in mouse and human airway epithelial cells","volume":"80","author":"Ibricevic","year":"2006","journal-title":"J. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.virol.2018.12.016","article-title":"Development and characterization of swine primary respiratory epithelial cells and their susceptibility to infection by four influenza virus types","volume":"528","author":"Sreenivasan","year":"2019","journal-title":"Virology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"114943","DOI":"10.1016\/j.jviromet.2024.114943","article-title":"Establishment of Swine Primary Nasal, Tracheal, and Bronchial Epithelial Cell Culture Models for the Study of Influenza Virus Infection","volume":"327","author":"Krunkosky","year":"2024","journal-title":"J. Virol. Methods"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Reeves, S.R., Barrow, K.A., White, M.P., Rich, L.M., Naushab, M., and Debley, J.S. (2018). Stability of gene expression by primary bronchial epithelial cells over increasing passage number. BMC Pulm. Med., 18.","DOI":"10.1186\/s12890-018-0652-2"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e01091-20","DOI":"10.1128\/JVI.01091-20","article-title":"Primary swine respiratory epithelial cell lines for the efficient isolation and propagation of influenza a viruses","volume":"94","author":"Meliopoulos","year":"2020","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Rijsbergen, L.C., van Dijk, L.L.A., Engel, M.F.M., de Vries, R.D., and de Swart, R.L. (2021). In vitro modelling of respiratory virus infections in human airway epithelial cells\u2014A systematic review. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.683002"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Thomas, M., Pierson, M., Uprety, T., Zhu, L., Ran, Z., Sreenivasan, C.C., Wang, D., Hause, B., Francis, D.H., and Li, F. (2018). Comparison of Porcine Airway and Intestinal Epithelial Cell Lines for the Susceptibility and Expression of Pattern Recognition Receptors upon Influenza Virus Infection. Viruses, 10.","DOI":"10.3390\/v10060312"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0166-0934(02)00236-7","article-title":"Establishment and characterization of two new pig cell lines for use in virological diagnostic laboratories","volume":"107","author":"Ferrari","year":"2003","journal-title":"J. Virol. Methods"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"9763","DOI":"10.1002\/jcp.26942","article-title":"Establishment and characterization of a telomerase-immortalized porcine bronchial epithelial cell line","volume":"233","author":"Xie","year":"2018","journal-title":"J. Cell Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Fukuyama, K., Zhuang, T., Toyoshi, E., Raya Tonetti, F., Saha, S., Zhou, B., Ikeda-Ohtsubo, W., Nishiyama, K., Aso, H., and Villena, J. (2023). Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1117102"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/1297-9716-45-42","article-title":"Innate immune response to a H3N2 subtype swine influenza virus in newborn porcine trachea cells, alveolar macrophages, and precision-cut lung slices","volume":"45","author":"Melo","year":"2014","journal-title":"Vet. Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wu, N.H., Yang, W., Beineke, A., Dijkman, R., Matrosovich, M., Baumg\u00e4rtner, W., Thiel, V., Valentin-Weigand, P., Meng, F., and Herrler, G. (2016). The differentiated airway epithelium infected by influenza viruses maintains the bar rier function despite a dramatic loss of ciliated cells. Sci. Rep., 6.","DOI":"10.1038\/srep39668"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"6844","DOI":"10.1128\/JVI.02200-10","article-title":"Viral replication and innate host responses in primary human alveolar epithelial cells and alveolar macrophages infected with influenza H5N1 and H1N1 viruses","volume":"85","author":"Yu","year":"2011","journal-title":"J. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1093\/infdis\/jis455","article-title":"Influenza A viruses target type II pneumocytes in the human lung","volume":"206","author":"Weinheimer","year":"2012","journal-title":"J. Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1093\/infdis\/jiy719","article-title":"Infection Studies in Pigs and Porcine Airway Epithelial Cells Reveal an Evolution of A(H1N1)pdm09 Influenza A Viruses Toward Lower Virulence","volume":"219","author":"Fu","year":"2019","journal-title":"J. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Villal\u00f3n-Letelier, F., Brooks, A.G., Saunders, P.M., Londrigan, S.L., and Reading, P.C. (2017). Host Cell Restriction Factors That Limit Influenza A Infection. Viruses, 9.","DOI":"10.3390\/v9120376"},{"key":"ref_24","unstructured":"Fields, B.N. (2007). Fields\u2019 Virology, Lippincott Williams & Wilkins."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1016\/j.biochi.2007.04.015","article-title":"Interferon-induced Mx proteins in antiviral host defense","volume":"89","author":"Haller","year":"2007","journal-title":"Biochimie"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"220","DOI":"10.20506\/rst.17.1.1084","article-title":"Mx proteins: Mediators of innate resistance to RNA viruses","volume":"17","author":"Haller","year":"1998","journal-title":"Rev. Sci. Tech."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.coviro.2015.07.002","article-title":"Inhibition of the OAS\/RNase L pathway by viruses","volume":"15","author":"Drappier","year":"2015","journal-title":"Curr. Opin. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"99","DOI":"10.4168\/aair.2022.14.1.99","article-title":"OASL1-Mediated Inhibition of Type I IFN Reduces Influenza A Infection-Induced Airway Inflammation by Regulating ILC2s","volume":"14","author":"Chang","year":"2022","journal-title":"Allergy Asthma Immunol. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"L1178","DOI":"10.1152\/ajplung.00290.2014","article-title":"Influenza induces IL-8 and GM-CSF secretion by human alveolar epithelial cells through HGF\/c-Met and TGF-\u03b1\/EGFR signaling","volume":"308","author":"Ito","year":"2015","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"116","DOI":"10.3109\/08820139.2015.1099663","article-title":"Up-Regulation of Pro-Inflammatory Cytokines and Chemokine Production in Avian Influenza H9N2 Virus-Infected Human Lung Epithelial Cell Line (A549)","volume":"45","author":"Farzin","year":"2016","journal-title":"Immunol. Investig."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1089\/vim.2010.0125","article-title":"Induction of inflammatory cytokines and toll-like receptors in human normal respiratory epithelial cells infected with seasonal H1N1, 2009 pandemic H1N1, seasonal H3N2, and highly pathogenic H5N1 influenza virus","volume":"24","author":"Song","year":"2011","journal-title":"Viral Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Alagarasu, K., Kaushal, H., Shinde, P., Kakade, M., Chaudhary, U., Padbidri, V., Sangle, S.A., Salvi, S., Bavdekar, A.R., and D\u2019costa, P. (2021). TNFA and IL10 Polymorphisms and IL-6 and IL-10 Levels Influence Disease Severity in Influenza A(H1N1)pdm09 Virus Infected Patients. Genes, 12.","DOI":"10.3390\/genes12121914"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Zhang, J., Wang, J., Gong, Y., Gu, Y., Xiang, Q., and Tang, L.L. (2022). Interleukin-6 and granulocyte colony-stimulating factor as predictors of the prognosis of influenza-associated pneumonia. BMC Infect. Dis., 22.","DOI":"10.1186\/s12879-022-07321-6"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Liu, S., Yan, R., Chen, B., Pan, Q., Chen, Y., Hong, J., Zhang, L., Liu, W., Wang, S., and Chen, J.L. (2019). Influenza Virus-Induced Robust Expression of SOCS3 Contributes to Excessive Production of IL-6. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.01843"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Yang, M.L., Wang, C.T., Yang, S.J., Leu, C.H., Chen, S.H., Wu, C.L., and Shiau, A.L. (2017). IL-6 ameliorates acute lung injury in influenza virus infection. Sci. Rep., 7.","DOI":"10.1038\/srep43829"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e0069221","DOI":"10.1128\/JVI.00692-21","article-title":"Innate Antiviral Cytokine Response to Swine Influenza Virus by Swine Respiratory Epithelial Cells","volume":"95","author":"Bakre","year":"2021","journal-title":"J. Virol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1002\/eji.201646484","article-title":"Negative regulators of the RIG-I-like receptor signaling pathway","volume":"47","author":"Quicke","year":"2017","journal-title":"Eur. J. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Maelfait, J., Roose, K., Vereecke, L., Mc Guire, C., Sze, M., Schuijs, M.J., Willart, M., Iba\u00f1ez, L.I., Hammad, H., and Lambrecht, B.N. (2016). A20 Deficiency in Lung Epithelial Cells Protects against Influenza A Virus Infection. PLoS Pathog., 12.","DOI":"10.1371\/journal.ppat.1005410"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/S1074-7613(00)80291-5","article-title":"Critical roles for the Bcl-3 oncoprotein in T cell-mediated immunity, splenic microarchitecture, and germinal center reactions","volume":"6","author":"Franzoso","year":"1997","journal-title":"Immunity"},{"key":"ref_40","first-page":"184","article-title":"MAP kinase phosphatase (MKP)-1 in the innate immune response to influenza infection (42.4)","volume":"1","author":"Wancket","year":"2010","journal-title":"J. Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1016\/j.bbrc.2016.11.166","article-title":"Influenza a virus NS1 protein induced A20 contributes to viral replication by suppressing interferon-induced antiviral response","volume":"482","author":"Feng","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.ejphar.2006.03.073","article-title":"An inhibitory effect of A20 on NF-kappaB activation in airway epithelium upon influenza virus infection","volume":"541","author":"Onose","year":"2006","journal-title":"Eur. J. Pharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/j.bbrc.2014.06.059","article-title":"Induction of the cellular miR-29c by influenza virus inhibits the innate immune response through protection of A20 mRNA","volume":"450","author":"Zhang","year":"2014","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Gu, Y., Hsu, A.C., Zuo, X., Guo, X., Zhou, Z., Jiang, S., Ouyang, Z., and Wang, F. (2023). Chronic exposure to low-level lipopolysaccharide dampens influenza-mediated inflammatory response via A20 and PPAR network. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1119473"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"269","DOI":"10.3892\/ijo.2012.1685","article-title":"Bcl-3 suppresses Tax-induced NF-\u03baB activation through p65 nuclear translocation blockage in HTLV-1-infected cells","volume":"42","author":"Wang","year":"2013","journal-title":"Int. J. Oncol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"15302","DOI":"10.1128\/JVI.79.24.15302-15313.2005","article-title":"Respiratory syncytial virus-inducible BCL-3 expression antagonizes the STAT\/IRF and NF-kappaB signaling pathways by inducing histone deacetylase 1 recruitment to the interleukin-8 promoter","volume":"79","author":"Jamaluddin","year":"2005","journal-title":"J. Virol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"16004","DOI":"10.1074\/jbc.M900146200","article-title":"Bcl-2 expression and p38MAPK activity in cells infected with influenza A virus: Impact on virally induced apoptosis and viral replication","volume":"284","author":"Nencioni","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"148768","DOI":"10.1016\/j.gene.2024.148768","article-title":"SIGIRR suppresses hepatitis B virus X protein-induced chronic inflammation in hepatocytes","volume":"928","author":"Ye","year":"2024","journal-title":"Gene"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1916","DOI":"10.1080\/15548627.2022.2162798","article-title":"The nucleoprotein of influenza A virus inhibits the innate immune response by inducing mitophagy","volume":"19","author":"Zhang","year":"2023","journal-title":"Autophagy"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1111\/cea.12793","article-title":"Tollip SNP rs5743899 modulates human airway epithelial responses to rhinovirus infection","volume":"46","author":"Huang","year":"2016","journal-title":"Clin. Exp. Allergy"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1159\/000497072","article-title":"Tollip inhibits ST2 signaling in airway epithelial cells exposed to type 2 cytokines and rhinovirus","volume":"12","author":"Dakhama","year":"2020","journal-title":"J. Innate Immun."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1159\/000525315","article-title":"Tollip Inhibits IL-33 Release and Inflammation in Influenza A Virus-Infected Mouse Airways","volume":"15","author":"Schaunaman","year":"2023","journal-title":"J. Innate Immun."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1016\/j.ajpath.2025.02.005","article-title":"TOLLIP downregulation by cigarette smoke exposure impairs human lung defense against Influenza A virus infection","volume":"195","author":"Agraval","year":"2025","journal-title":"Am. J. Pathol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1007\/s11033-010-0146-7","article-title":"SIGIRR inhibits toll-like receptor 4, 5, 9-mediated immune responses in human airway epithelial cells","volume":"38","author":"Zhang","year":"2011","journal-title":"Mol. Biol. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Ueno-Shuto, K., Kamei, S., Hayashi, M., Fukuyama, A., Uchida, Y., Tokutomi, N., Suico, M.A., Kai, H., and Shuto, T. (2022). A Splice Switch in SIGIRR Causes a Defect of IL-37-Dependent Anti-Inflammatory Activity in Cystic Fibrosis Airway Epithelial Cells. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23147748"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Cumplido-Laso, G., Benitez, D.A., Mulero-Navarro, S., and Carvajal-Gonzalez, J.M. (2023). Transcriptional Regulation of Airway Epithelial Cell Differentiation: Insights into the Notch Pathway and Beyond. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241914789"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"L603","DOI":"10.1152\/ajplung.00283.2020","article-title":"First contact: The role of respiratory cilia in host-pathogen interactions in the airways","volume":"319","author":"Kuek","year":"2020","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/12\/5764\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:52:55Z","timestamp":1760032375000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/12\/5764"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,16]]},"references-count":57,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["ijms26125764"],"URL":"https:\/\/doi.org\/10.3390\/ijms26125764","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,16]]}}}