{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T11:56:52Z","timestamp":1789041412502,"version":"build-2803163510"},"reference-count":48,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T00:00:00Z","timestamp":1750291200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Project of the Natural Science Foundation of Xinjiang Uygur Autonomous Region in China","award":["2021D01D14"],"award-info":[{"award-number":["2021D01D14"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study aimed to investigate the preventive effects of isostrictiniin (ITN) from Nymphaea candida against acute lung injury (ALI) through lipopolysaccharide (LPS)-induced ALI mice and LPS-induced A549 cells. Compared with the model group, ITN (50 and 100 mg\/kg) significantly reduced the lung indexes, W\/D rates, BALF WBC counts, and total protein contents in ALI mice (p &lt; 0.05), as well as the blood neu counts (p &lt; 0.01), while increasing the blood lym counts (p &lt; 0.01). ITN (50 and 100 mg\/kg) also markedly decreased the lung tissue TNF-\u03b1, IL-6, IL-1\u03b2, MDA, and MPO activities in ALI mice (p &lt; 0.01) and enhanced the SOD and GSH levels (p &lt; 0.01). Additionally, ITN (50 and 100 mg\/kg) significantly improved lung histopathological damage in ALI mice. Moreover, ITN (10 and 25 \u00b5M) significantly reduced the NO, PGE2, IL-1\u03b2, IL-6, TNF-\u03b1, and MDA levels in LPS-induced A549 cells (p &lt; 0.01) while significantly increasing the SOD and GSH activities (p &lt; 0.01). After LPS-induced A549 cells, the Keap1, p-JNK\/JNK, p-ERK1\/2\/ERK1\/2, p-P38\/P38, p-I\u03baB\u03b1\/I\u03baB\u03b1, and p-NF-\u03baBp65\/NF-\u03baB p65 levels were significantly upregulated (p &lt; 0.05), whereas the Nrf2 and HO-1 protein expressions were downregulated (p &lt; 0.05). After treatment with ITN (25 \u03bcM), the changes in these relative protein expressions in LPS-induced A549 cells were significantly reversed (p &lt; 0.05). The above results indicate that ITN has a better preventive effect against ALI, and its mechanisms are related to the regulation of the Keap1-Nrf2\/HO-1 and MAPK\/NF-\u03baB signaling pathways.<\/jats:p>","DOI":"10.3390\/ijms26125912","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T03:05:27Z","timestamp":1750388727000},"page":"5912","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Isostrictiniin Alleviates LPS-Induced Acute Lung Injury via the Regulation of the Keap1-Nrf2\/HO-1 and MAPK\/NF-\u03baB Signaling Pathways"],"prefix":"10.3390","volume":"26","author":[{"given":"Wanting","family":"Ding","sequence":"first","affiliation":[{"name":"School of Pharmacy, Xinjiang Medical University, Urumqi 830011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Xinjiang Medical University, Urumqi 830011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wulipan","family":"Tuohudaali","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Xinjiang Medical University, Urumqi 830011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9984-0541","authenticated-orcid":false,"given":"Chenyang","family":"Li","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory for Uighur Medicine, Institute of Materia Medica of Xinjiang, Urumqi 830004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Yao","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory for Uighur Medicine, Institute of Materia Medica of Xinjiang, Urumqi 830004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9890-4582","authenticated-orcid":false,"given":"Jun","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Xinjiang Medical University, Urumqi 830011, China"},{"name":"Xinjiang Key Laboratory for Uighur Medicine, Institute of Materia Medica of Xinjiang, Urumqi 830004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,19]]},"reference":[{"key":"ref_1","first-page":"914","article-title":"Targeting TLR4 and regulating the Keap1\/Nrf2 pathway with andrographolide to suppress inflammation and ferroptosis in LPS-induced acute lung injury","volume":"22","author":"Yichen","year":"2024","journal-title":"China J. 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