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(DAP) is a therapeutic option for vancomycin-resistant Enterococcus faecium (VRE) infections, but DAP resistance may occur during treatment. Previously, we identified a mutation within the E. faecium lafB gene that induces hypersusceptibility to DAP. The lafB gene encodes a glycosyltransferase involved in lipoteichoic acid anchor synthesis, which makes it a promising target for enhancing DAP efficacy. In this study, we characterized E. faecium LafB protein (EfLafB) biophysical properties, used AlphaFold3 to predict LafB in silico three-dimensional structure, and determined lafB gene mutation\u2019s role in virulence, comparing E. faecium HBSJRP18 (DAP-hypersusceptible) and a lafB revertant, HBSJRP18_2.7, and analyzing bacterial growth kinetics, biofilm formation ability, and virulence in a Galleria mellonella model. After gene cloning and expressing and purifying EfLafB, circular dichroism and SEC-MALS assays revealed its monomeric nature under in vitro conditions, with approximately a 40 kDa molecular mass and a melting temperature of 50 \u00b0C. In silico prediction indicated that LafB is an \u03b1\u03b2-type protein with two domains conforming to the GT-4 family glycosyltransferases. These results are further supported by the highly conserved amino acids (E257, D91, R184, and K185), likely involved in UDP-Glc binding. The studied lafB gene mutation resulted in a significant decrease in bacterial growth and virulence in the invertebrate model.<\/jats:p>","DOI":"10.3390\/ijms26135935","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T09:04:49Z","timestamp":1750410289000},"page":"5935","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring Daptomycin Hypersensitivity in Enterococcus faecium: The Impact of LafB Mutation on Bacterial Virulence"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1488-827X","authenticated-orcid":false,"given":"Pamela I. Huanambal","family":"Esqu\u00e9n","sequence":"first","affiliation":[{"name":"Department of Physics and Interdisciplinary Science, Sao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos 13563-120, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4264-3074","authenticated-orcid":false,"given":"Diego A.","family":"Leonardo","sequence":"additional","affiliation":[{"name":"Department of Physics and Interdisciplinary Science, Sao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos 13563-120, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2845-4958","authenticated-orcid":false,"given":"Livia R.","family":"Manzine","sequence":"additional","affiliation":[{"name":"Department of Physics and Interdisciplinary Science, Sao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos 13563-120, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erick Suclupe","family":"Farro","sequence":"additional","affiliation":[{"name":"Department of Physics and Interdisciplinary Science, Sao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos 13563-120, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica K.","family":"Kajfasz","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, University of Florida College of Dentistry, Gainsville, FL 32610, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6389-6041","authenticated-orcid":false,"given":"Suelen S.","family":"Mello","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mara C. L.","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Departamento de Doen\u00e7as Dermatol\u00f3gicas, Infecciosas e Parasit\u00e1rias, Faculdade de Medicina de S\u00e3o Jos\u00e9 do Rio Preto\u2014FAMERP, S\u00e3o Jos\u00e9 do Rio Preto 15090-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2731-3966","authenticated-orcid":false,"given":"Jo\u00e3o Renato","family":"Muniz","sequence":"additional","affiliation":[{"name":"Department of Physics and Interdisciplinary Science, Sao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos 13563-120, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6656-5379","authenticated-orcid":false,"given":"Alessandro S.","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Department of Physics and Interdisciplinary Science, Sao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos 13563-120, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael S.","family":"Gilmore","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline","family":"Abranches","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, University of Florida College of Dentistry, Gainsville, FL 32610, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 A.","family":"Lemos","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, University of Florida College of Dentistry, Gainsville, FL 32610, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4489-1215","authenticated-orcid":false,"given":"Ilana L. 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