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The growing threat of drug resistance to artemisinin-based combination therapies (ACTs) jeopardizes progress toward elimination. This study examined P. falciparum samples collected from 141 patients in Brazil (2013\u20132023) by PCR and DNA sequencing to identify single-nucleotide polymorphisms in the pfcrt, pfmdr1, and pfk13 genes. Half of the samples carried the SVMNTMCGI haplotype in pfcrt, and none of the samples showed C350R mutations. In pfmdr1, the NYCDY haplotype was dominant (70%), with low occurrences of N86Y (4%) and no Y184F polymorphisms. No mutations linked to artemisinin partial resistance were detected in pfk13. Only one Amazonas sample exhibited wild-type haplotypes across all genes. Genetic diversity was more pronounced in pfcrt than pfmdr1, reflecting selective drug pressure. Significant linkage disequilibrium (LD) was observed within pfcrt (C72S and K76T) and pfmdr1 (S1034C and N1042D), but not between the two genes. The absence of pfk13-resistant mutations and the low prevalence of key pfmdr1 markers support the efficacy of ACTs. The persistence of diverse haplotypes and intragenic LD reflects ongoing drug pressure, underscoring the need for continuous genetic surveillance to anticipate emerging resistance.<\/jats:p>","DOI":"10.3390\/ijms26135977","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T09:08:44Z","timestamp":1750669724000},"page":"5977","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Tracking Drug Resistance in Plasmodium falciparum: Genetic Diversity of Key Resistance Markers in Brazilian Malaria Hotspots"],"prefix":"10.3390","volume":"26","author":[{"given":"Rebecca","family":"de Abreu-Fernandes","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Mal\u00e1ria, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21041-361, Brazil"},{"name":"Centro de Pesquisa, Diagn\u00f3stico e Treinamento em Mal\u00e1ria (CPD-Mal), Reference 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Med. Surg."},{"key":"ref_2","unstructured":"World Health Organization (2024). World Malaria Report 2024, World Health Organization. Available online: https:\/\/www.who.int\/teams\/global-malaria-programme\/reports\/world-malaria-report-2024."},{"key":"ref_3","unstructured":"Tableau Public (2025, January 13). Malaria 2024. Available online: https:\/\/public.tableau.com\/app\/profile\/mal.ria.brasil\/viz\/Dadosparacidado_201925_03_2020\/Incio."},{"key":"ref_4","unstructured":"Minist\u00e9rio da Sa\u00fade (2021). Guia de tratamento da mal\u00e1ria no Brasil; Secretaria de Vigil\u00e2ncia em Sa\u00fade, Departamento de Imuniza\u00e7\u00e3o e Doen\u00e7as Transmiss\u00edveis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1056\/NEJMp1403340","article-title":"The origins of antimalarial-drug resistance","volume":"371","author":"Packard","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.pt.2013.08.002","article-title":"Mapping \u2018partially resistant\u2019, \u2018fully resistant\u2019, and \u2018super resistant\u2019 malaria","volume":"29","author":"Naidoo","year":"2013","journal-title":"Trends Parasitol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"369","DOI":"10.3347\/kjp.2019.57.4.369","article-title":"Molecular Surveillance of Pfkelch13 and Pfmdr1 Mutations in Plasmodium falciparum Isolates from Southern Thailand","volume":"57","author":"Khammanee","year":"2019","journal-title":"Korean J. Parasitol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1056\/NEJMoa1314981","article-title":"Spread of artemisinin resistance in Plasmodium falciparum malaria","volume":"371","author":"Ashley","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"11672","DOI":"10.1073\/pnas.1507142112","article-title":"Adaptive evolution of malaria parasites in French Guiana: Reversal of chloroquine resistance by acquisition of a mutation in pfcrt","volume":"112","author":"Pelleau","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2619","DOI":"10.1056\/NEJMc0805011","article-title":"Evidence of artemisinin-resistant malaria in western Cambodia","volume":"359","author":"Noedl","year":"2008","journal-title":"N. Engl. J. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1186\/s12936-015-0892-z","article-title":"Falciparum malaria molecular drug resistance in the Democratic Republic of Congo: A systematic review","volume":"14","author":"Mvumbi","year":"2015","journal-title":"Malar. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/s12936-021-03613-5","article-title":"Molecular surveillance of pfcrt, pfmdr1, and pfk13-propeller mutations in Plasmodium falciparum isolates imported from Africa to China","volume":"20","author":"Huang","year":"2021","journal-title":"Malar. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/nature12876","article-title":"A molecular marker of artemisinin-resistant Plasmodium falciparum malaria","volume":"505","author":"Ariey","year":"2014","journal-title":"Nature"},{"key":"ref_14","unstructured":"World Health Organization (2025, June 18). Malaria: Artemisinin Partial Resistance [Internet], Available online: https:\/\/www.who.int\/news-room\/questions-and-answers\/item\/artemisinin-resistance."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Zhao, Y., Liu, Z., Soe, M.T., Wang, L., Soe, T.N., Wei, H., Than, A., Aung, P.L., Li, Y., and Zhang, X. (2019). Genetic Variations Associated with Drug Resistance Markers in Asymptomatic Plasmodium falciparum Infections in Myanmar. Genes, 10.","DOI":"10.3390\/genes10090692"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"de Abreu-Fernandes, R., Almeida-de-Oliveira, N.K., Gama, B.E., Gomes, L.R., De Lavigne Mello, A.R., Queiroz, L.T., Barros, J.A., Alecrim, M.D.G.C., Medeiros de Souza, R., and Pratt-Riccio, L.R. (2023). Plasmodium falciparum Chloroquine-pfcrt Resistant Haplotypes in Brazilian Endemic Areas Four Decades after CQ Withdrawn. Pathogens, 12.","DOI":"10.3390\/pathogens12050731"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e00303-17","DOI":"10.1128\/AAC.02655-16","article-title":"Molecular profile of malaria drug resistance markers of Plasmodium falciparum in Suriname","volume":"61","author":"Chenet","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1186\/s12936-024-05107-6","article-title":"Molecular survey of pfmdr-1, pfcrt, and pfk13 gene mutations among patients returning from Plasmodium falciparum endemic areas to Turkey","volume":"23","year":"2024","journal-title":"Malar. J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"11553","DOI":"10.1038\/ncomms11553","article-title":"Globally prevalent PfMDR1 mutations modulate Plasmodium falciparum susceptibility to artemisinin-based combination therapies","volume":"7","author":"Veiga","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1128\/AAC.00968-08","article-title":"Selection of Plasmodium falciparum multidrug resistance gene 1 alleles in asexual stages and gametocytes by artemether-lumefantrine in Nigerian children with uncomplicated falciparum malaria","volume":"53","author":"Happi","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Ndong Ngomo, J.M., Mawili-Mboumba, D.P., M\u2019Bondoukw\u00e9, N.P., Ditombi, B.M., Koumba Lengongo, J.V., Batchy Ognagosso, F.B., and Bouyou-Akotet, M.K. (2023). Drug Resistance Molecular Markers of Plasmodium falciparum and Severity of Malaria in Febrile Children in the Sentinel Site for Malaria Surveillance of Melen in Gabon: Additional Data from the Plasmodium Diversity Network African Network. Trop. Med. Infect. Dis., 8.","DOI":"10.3390\/tropicalmed8040184"},{"key":"ref_22","first-page":"7071383","article-title":"Effects of Drug Policy Changes on Evolution of Molecular Markers of Plasmodium falciparum Resistance to Chloroquine, Amodiaquine, and Sulphadoxine-Pyrimethamine in the South West Region of Cameroon","volume":"2018","author":"Moyeh","year":"2018","journal-title":"Malar. Res. Treat."},{"key":"ref_23","unstructured":"Global Malaria Programme (2018). Artemisinin Resistance and Artemisinin-Based Combination Therapy Efficacy, Status Report, World Health Organization. Available online: https:\/\/iris.who.int\/handle\/10665\/274362."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1590\/0074-0276130579","article-title":"Emerging Plasmodium vivax resistance to chloroquine in South America: An overview","volume":"109","author":"Cravo","year":"2014","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"MalariaGEN Plasmodium falciparum Community Project (2016). Genomic epidemiology of artemisinin-resistant malaria. eLife, 5, e08714.","DOI":"10.7554\/eLife.08714"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1186\/1475-2875-9-355","article-title":"Brazilian Plasmodium falciparum isolates: Investigation of candidate polymorphisms for artemisinin resistance before the introduction of artemisinin-based combination therapy","volume":"9","author":"Gama","year":"2010","journal-title":"Malar. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e00354-18","DOI":"10.1128\/AAC.00354-18","article-title":"Absence of K13 polymorphism in Plasmodium falciparum from Brazilian areas where the parasite is endemic","volume":"62","author":"Gomes","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2854","DOI":"10.1093\/jac\/dkab264","article-title":"Kelch13 mutations in Plasmodium falciparum and risk of spreading in Amazon basin countries","volume":"76","author":"Mathieu","year":"2021","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"de Aguiar-Barros, J., Granja, F., de Abreu-Fernandes, R., de Queiroz, L.T., da Silva, E.S., Cit\u00f3, A.C., Mocelin, N.K.A., Daniel-Ribeiro, C.T., and Ferreira-da-Cruz, M.F. (2024). Molecular surveillance of artemisinin-resistant Plasmodium falciparum parasites in mining areas of the Roraima Indigenous Territory in Brazil. Int. J. Environ. Res. Public Health, 21.","DOI":"10.20944\/preprints202404.1822.v1"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/ng.3189","article-title":"Genetic architecture of artemisinin-resistant Plasmodium falciparum","volume":"47","author":"Miotto","year":"2015","journal-title":"Nat Genet."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.4269\/ajtmh.19-0954","article-title":"Artemether-Lumefantrine Efficacy for the Treatment of Uncomplicated Plasmodium falciparum Malaria in Choco, Colombia after 8 Years as First-Line Treatment","volume":"102","author":"Olivera","year":"2020","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e20180225","DOI":"10.1590\/0037-8682-0225-2018","article-title":"Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene","volume":"52","author":"Chapadense","year":"2019","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1186\/s12936-016-1306-6","article-title":"Molecular mutation profile of pfcrt in Plasmodium falciparum isolates imported from Africa in Henan Province","volume":"15","author":"Zhou","year":"2016","journal-title":"Malar. J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1186\/1475-2875-13-372","article-title":"Analysis of polymorphisms in Plasmodium falciparum genes related to drug resistance: A survey over four decades under different treatment policies in Brazil","volume":"13","author":"Inoue","year":"2014","journal-title":"Malar. J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1186\/s12936-017-1806-z","article-title":"Assessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literature","volume":"16","author":"Costa","year":"2017","journal-title":"Malar. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1016\/j.jiph.2022.03.015","article-title":"Molecular surveillance of chloroquine-resistant Plasmodium falciparum in sub-Saharan African countries after withdrawal of chloroquine for treatment of uncomplicated malaria: A systematic review","volume":"15","author":"Njiro","year":"2022","journal-title":"J. Infect. Public Health"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1186\/s12936-020-03281-x","article-title":"Prevalence of mutations in the Plasmodium falciparum chloroquine resistance transporter, PfCRT, and association with ex vivo susceptibility to common anti-malarial drugs against African Plasmodium falciparum isolates","volume":"19","author":"Foguim","year":"2020","journal-title":"Malar. J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3314","DOI":"10.1038\/s41467-018-05652-0","article-title":"Emerging Southeast Asian PfCRT mutations confer Plasmodium falciparum resistance to the first-line antimalarial piperaquine","volume":"9","author":"Ross","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1186\/s12936-016-1503-3","article-title":"Selection of Plasmodium falciparum pfcrt and pfmdr1 polymorphisms after treatment with artesunate-amodiaquine fixed-dose combination or artemether-lumefantrine in Liberia","volume":"15","author":"Otienoburu","year":"2016","journal-title":"Malar. J."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1186\/s12936-016-1490-4","article-title":"Selection of N86F184D1246 haplotype of pfmdr1 gene by artemether-lumefantrine drug pressure on Plasmodium falciparum populations in Senegal","volume":"15","author":"Mbaye","year":"2016","journal-title":"Malar. J."},{"key":"ref_41","first-page":"310","article-title":"Molecular surveillance for polymorphisms associated with artemisinin-based combination therapy resistance in Plasmodium falciparum isolates collected in the state of Roraima, Brazil","volume":"102","author":"Lucchi","year":"2020","journal-title":"Malar. J."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Adam, R., Mukhtar, M.M., Abubakar, U.F., Damudi, H.A., Muhammad, A., and Ibrahim, S.S. (2021). Polymorphism Analysis of pfmdr1 and pfcrt from Plasmodium falciparum Isolates in Northwestern Nigeria Revealed the Major Markers Associated with Antimalarial Resistance. Diseases, 9.","DOI":"10.3390\/diseases9010006"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1590\/S0074-02762011000100013","article-title":"Sequence analysis of coding DNA fragments of pfcrt and pfmdr-1 genes in Plasmodium falciparum isolates from Odisha, India","volume":"106","author":"Sutar","year":"2011","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1186\/s12936-020-03247-z","article-title":"Malaria in Venezuela: Changes in the complexity of infection reflects the increment in transmission intensity","volume":"19","author":"Pacheco","year":"2020","journal-title":"Malar. J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"106190","DOI":"10.1016\/j.ijantimicag.2020.106190","article-title":"Absence of association between polymorphisms in the pfcoronin and pfk13 genes and the presence of Plasmodium falciparum parasites after treatment with artemisinin derivatives in Senegal","volume":"56","author":"Delandre","year":"2020","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e01320-21","DOI":"10.1128\/AAC.01320-21","article-title":"Mutation in the Plasmodium falciparum BTB\/POZ domain of K13 protein confers artemisinin resistance","volume":"66","author":"Paloque","year":"2022","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.exppara.2007.02.011","article-title":"Real-Time PCR versus Conventional PCR for Malaria Parasite Detection in Low-Grade Parasitemia","volume":"116","author":"Gama","year":"2007","journal-title":"Exp. Parasitol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e180350","DOI":"10.1590\/0074-02760180350","article-title":"Analytical Validation of Real-Time Quantitative PCR Assays for Optimum Diagnosis of Vivax Malaria","volume":"114","author":"Moreira","year":"2019","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1007\/s004360050173","article-title":"Malaria Diagnosis: Standardization of a Polymerase Chain Reaction for the Detection of Plasmodium falciparum Parasites in Individuals with Low-Grade Parasitemia","volume":"82","author":"Zalis","year":"1996","journal-title":"Parasitol. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/0166-6851(93)90077-B","article-title":"High Sensitivity of Detection of Human Malaria Parasites by the Use of Nested Polymerase Chain Reaction","volume":"61","author":"Snounou","year":"1993","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_51","first-page":"95","article-title":"BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp. Ser."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1093\/bioinformatics\/btp187","article-title":"DnaSP v5: A software for comprehensive analysis of DNA polymorphism data","volume":"25","author":"Librado","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1093\/bioinformatics\/bth457","article-title":"Haploview: Analysis and visualization of LD and haplotype maps","volume":"21","author":"Barrett","year":"2005","journal-title":"Bioinformatics"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/13\/5977\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:56:21Z","timestamp":1760032581000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/13\/5977"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,21]]},"references-count":53,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["ijms26135977"],"URL":"https:\/\/doi.org\/10.3390\/ijms26135977","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,21]]}}}