{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:29:01Z","timestamp":1760059741196,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Science Foundation","award":["20-14-00074-P","075-15-2022-310","Priority-2030"],"award-info":[{"award-number":["20-14-00074-P","075-15-2022-310","Priority-2030"]}]},{"name":"Ministry of Science and Higher Education of the Russian Federation","award":["20-14-00074-P","075-15-2022-310","Priority-2030"],"award-info":[{"award-number":["20-14-00074-P","075-15-2022-310","Priority-2030"]}]},{"name":"Tomsk State University Development Program","award":["20-14-00074-P","075-15-2022-310","Priority-2030"],"award-info":[{"award-number":["20-14-00074-P","075-15-2022-310","Priority-2030"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The strategy of active surveillance for papillary thyroid microcarcinoma (PTMC) is becoming increasingly popular within the global medical community. A key criterion for selecting this strategy is the absence of any signs of lymphogenic or distant metastases. The present study assessed the diagnostic accuracy of molecular genetic markers for predicting the metastatic potential of patients with PTMC. We evaluated the expression levels of 33 molecular genetic markers in cytology samples from 92 patients with PTMC and confirmed histological diagnosis. Among these patients, 32 had metastases to regional cervical lymph nodes. Our findings revealed the upregulated expression of the HMGA2, TIMP1, and FN1 genes, as well as microRNA-146b, in patients with metastatic PTMC. Conversely, we found the downregulated expression of miRNA-7 and -148b in metastatic tumors. In metastatic tumors, significant reductions were observed in DIO1 activity (11-fold), TFF3 gene expression (8-fold), TPO expression (4-fold), and SLC26A7 expression (2.6-fold). All the markers exhibited high sensitivity (84.5\u201390.6%) in detecting metastatic PTMC, although the specificity proved to be low. The use of molecular markers to predict lymphogenic metastatic spread in patients with PTMC could enhance existing risk grading systems. Such assessments can already be applicable at the preoperative stage.<\/jats:p>","DOI":"10.3390\/ijms26136418","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T08:22:07Z","timestamp":1751530927000},"page":"6418","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Predicting the Metastatic Potential of Papillary Thyroid Microcarcinoma Based on the Molecular Profile of Preoperative Cytology Specimens"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5559-9872","authenticated-orcid":false,"given":"Sergei A.","family":"Lukyanov","sequence":"first","affiliation":[{"name":"Department of General Surgery, South Ural State Medical University, 454141 Chelyabinsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9401-5737","authenticated-orcid":false,"given":"Sergei E.","family":"Titov","sequence":"additional","affiliation":[{"name":"Department of the Structure and Function of Chromosomes, Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia"},{"name":"AO Vector-Best, 630117 Novosibirsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aria V.","family":"Dzodzaeva","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Endocrinology, 115478 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir E.","family":"Vanushko","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Endocrinology, 115478 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitry G.","family":"Beltsevich","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Endocrinology, 115478 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3799-9407","authenticated-orcid":false,"given":"Yuliya A.","family":"Veryaskina","sequence":"additional","affiliation":[{"name":"Department of the Structure and Function of Chromosomes, Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia"},{"name":"Laboratory of Gene Engineering, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Semyon V.","family":"Kupriyanov","sequence":"additional","affiliation":[{"name":"Laboratory of Evolutionary Cytogenetics, National Research Tomsk State University, 634050 Tomsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2122-2297","authenticated-orcid":false,"given":"Ekaterina V.","family":"Bondarenko","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Endocrinology, 115478 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina A.","family":"Troshina","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Endocrinology, 115478 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6891-0009","authenticated-orcid":false,"given":"Liliya S.","family":"Urusova","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Endocrinology, 115478 Moscow, Russia"},{"name":"Institute of Clinical Morphology and Digital Pathology, I.M. Sechenov First Moscow State Medical University, 119991 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergei V.","family":"Sergiyko","sequence":"additional","affiliation":[{"name":"Department of General Surgery, South Ural State Medical University, 454141 Chelyabinsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e208759","DOI":"10.1001\/jamanetworkopen.2020.8759","article-title":"Global Burden of Thyroid Cancer From 1990 to 2017","volume":"3","author":"Deng","year":"2020","journal-title":"JAMA Netw. Open"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101630","DOI":"10.1016\/j.beem.2022.101630","article-title":"Active surveillance of low-risk papillary thyroid microcarcinoma","volume":"37","author":"Sugitani","year":"2023","journal-title":"Best Pract. Res. Clin. Endocrinol. 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