{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:28:02Z","timestamp":1787318882599,"version":"build-2736575974"},"reference-count":58,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Ultraviolet B (UVB), a component of solar ultraviolet light, is a major contributor to skin photodamage. UVB exposure primarily affects the epidermis, which leads to wrinkle formation, loss of skin elasticity, oxidative stress, and inflammation. Prolonged or intense UVB exposure can increase the risk of skin cancer. Collagen peptides are known as functional foods that improve skin dryness and wound healing. In this study, we aimed to investigate the protective and ameliorative effects of a low-molecular-weight collagen peptide (LMWCP) with a high absorption rate and photodamage. In vitro analysis using human dermal fibroblasts (HDFs) demonstrated that LMWCP promoted skin protection by increasing procollagen type I production, enhancing cell proliferation and migration, and inhibiting MMP-1 activity. Furthermore, LMWCP intake was indicated by improved skin hydration, reduced trans-epidermal water loss (TEWL), and changes in the clinical parameters, including skin elasticity, erythema, and scaling scores in UVB-exposed hairless mice. In the UVB-damaged tissues, an increase in skin elasticity-related enzymes was observed along with a decrease in aging-related and pro-inflammatory gene expression. Histological analysis revealed an increase in collagen content and restoration of dermal thickness. These findings suggested that LMWCP has significant benefits in preventing and improving UVB-induced skin damage.<\/jats:p>","DOI":"10.3390\/ijms26136427","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T09:57:39Z","timestamp":1751536659000},"page":"6427","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Low-Molecular-Weight Collagen Peptide Improves Skin Dehydration and Barrier Dysfunction in Human Dermal Fibrosis Cells and UVB-Exposed SKH-1 Hairless Mice"],"prefix":"10.3390","volume":"26","author":[{"given":"Eunjung","family":"Choi","sequence":"first","affiliation":[{"name":"Health Food Research and Development, NEWTREE Co., Ltd., Seoul 05604, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heeyeon","family":"Joo","sequence":"additional","affiliation":[{"name":"Health Food Research and Development, NEWTREE Co., Ltd., Seoul 05604, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myunghee","family":"Kim","sequence":"additional","affiliation":[{"name":"Health Food Research and Development, NEWTREE Co., Ltd., Seoul 05604, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do-Un","family":"Kim","sequence":"additional","affiliation":[{"name":"Health Food Research and Development, NEWTREE Co., Ltd., Seoul 05604, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2467-0327","authenticated-orcid":false,"given":"Hee-Chul","family":"Chung","sequence":"additional","affiliation":[{"name":"Health Food Research and Development, NEWTREE Co., Ltd., Seoul 05604, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4764-7640","authenticated-orcid":false,"given":"Jae Gon","family":"Kim","sequence":"additional","affiliation":[{"name":"INVIVO Co., Ltd., 121, Deahak-ro, Nonsan 32992, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"key":"ref_1","first-page":"4.1","article-title":"Functions of the Skin","volume":"Volume 1","author":"Burns","year":"2004","journal-title":"Rook\u2019s Textbook of Dermatology"},{"key":"ref_2","unstructured":"Geerligs, M. (2010). Skin Layer Mechanics, Technische Universiteit Eindhoven."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/S0029-6465(22)02423-9","article-title":"Skin anatomy, physiology, and pathophysiology","volume":"34","author":"Wysocki","year":"1999","journal-title":"Nurs. Clin. N. Am."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Lloyd, D.H., and Patel, A. (2012). Structure and function of the skin. BSAVA Manual of Canine and Feline Dermatology, BSAVA Library.","DOI":"10.22233\/9781905319886.1"},{"key":"ref_5","first-page":"159","article-title":"What is the \u2018true\u2019 function of skin?","volume":"11","author":"Kligman","year":"2002","journal-title":"Exp. Dermatol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Yadav, N., Parveen, S., Chakravarty, S., Banerjee, M.J.S.A., and Exposure, C.A.U.-R. (2019). Skin Anatomy and Morphology, Springer.","DOI":"10.1007\/978-981-13-2541-0_1"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.jphotobiol.2016.09.007","article-title":"Commercial sunscreen formulations: UVB irradiation stability and effect on UVB irradiation-induced skin oxidative stress and inflammation","volume":"163","author":"Vilela","year":"2016","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Slominski, R.M., Chander, R., Jetten, A.M., and Slominski, A.T. (2025). Neuro\u2013immuno\u2013endocrinology of the skin: How environment regulates body homeostasis. Nat. Rev. Endocrinol., 1\u201315.","DOI":"10.1038\/s41574-025-01107-x"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e2308374121","DOI":"10.1073\/pnas.2308374121","article-title":"Photo-neuro-immuno-endocrinology: How the ultraviolet radiation regulates the body, brain, and immune system","volume":"121","author":"Slominski","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Balasubramanian, P., Prabhakaran, M.P., Sireesha, M., and Ramakrishna, S. (2013). Collagen in human tissues: Structure, function, and biomedical implications from a tissue engineering perspective. Polymer Composites\u2013Polyolefin Fractionation\u2013Polymeric Peptidomimetics\u2013Collagens, Springer.","DOI":"10.1007\/12_2012_176"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Geng, R., Kang, S.-G., Huang, K., and Tong, T.J.N. (2021). Boosting the photoaged skin: The potential role of dietary components. Nutrients, 13.","DOI":"10.3390\/nu13051691"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1002\/ptr.6538","article-title":"Recent advances in the anti-aging effects of phytoestrogens on collagen, water content, and oxidative stress","volume":"34","author":"Liu","year":"2020","journal-title":"Phytother. Res."},{"key":"ref_13","first-page":"209","article-title":"Skin aging & modern age anti-aging strategies","volume":"7","author":"Mohiuddin","year":"2019","journal-title":"Int. J. Clin. Dermatol. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3008","DOI":"10.1039\/D3FO04949C","article-title":"Effects of collagen hydrolysates on UV-induced photoaging mice: Gly-Pro-Hyp as a potent anti-photoaging peptide","volume":"15","author":"Yang","year":"2024","journal-title":"Food Funct."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5936","DOI":"10.1039\/D3FO01516E","article-title":"The combined effect of commercial tilapia collagen peptides and antioxidants against UV-induced skin photoaging in mice","volume":"14","author":"Song","year":"2023","journal-title":"Food Funct."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Woo, H., Jeong, G.A., Choi, H., and Lee, C.J. (2023). Characterization of low-molecular-weight collagen from Korean native chicken feet hydrolyzed using alcalase. J. Microbiol. Biotechnol., 33.","DOI":"10.4014\/jmb.2212.12047"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1111\/jocd.16026","article-title":"Low-molecular-weight collagen peptides supplement promotes a healthy skin: A randomized, double-blinded, placebo-controlled study","volume":"23","author":"Seong","year":"2024","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1007\/s00726-021-03072-x","article-title":"The effects of collagen peptide supplementation on body composition, collagen synthesis, and recovery from joint injury and exercise: A systematic review","volume":"53","author":"Khatri","year":"2021","journal-title":"Amino Acids"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"299","DOI":"10.13103\/JFHS.2024.39.4.299","article-title":"Comparing Molecular Weight Dependent Absorption Rates of Collagen in Oral Mucosal and Epidermis\/dermis Tissue Models","volume":"39","author":"Hong","year":"2024","journal-title":"J. Food Hyg. Saf."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Chae, M., Bae, I.-H., Lim, S., Jung, K., Roh, J., and Kim, W. (2021). AP collagen peptides prevent cortisol-induced decrease of collagen type I in human dermal fibroblasts. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22094788"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1002\/fft2.373","article-title":"A comprehensive review of specific activity and intrinsic connections of food-derived bioactive peptides for human health","volume":"5","author":"Zhao","year":"2024","journal-title":"Food Front."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1097\/JD9.0000000000000068","article-title":"Pathogenesis of photoaging in human dermal fibroblasts","volume":"3","author":"Lee","year":"2020","journal-title":"Int. J. Dermatol. Venereol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1111\/j.0022-202X.2004.22614.x","article-title":"Reduced fibroblast interaction with intact collagen as a mechanism for depressed collagen synthesis in photodamaged skin","volume":"122","author":"Varani","year":"2004","journal-title":"J. Investig. Dermatol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/0012-1606(81)90149-4","article-title":"Role of collagen and fibronectin in neural crest cell adhesion and migration","volume":"87","author":"Greenberg","year":"1981","journal-title":"Dev. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Xu, Q., Hou, W., Zheng, Y., Liu, C., Gong, Z., Lu, C., Lai, W., and Maibach, H.I. (2014). Ultraviolet A-induced cathepsin K expression is mediated via MAPK\/AP-1 pathway in human dermal fibroblasts. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0102732"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.imlet.2012.09.005","article-title":"The anti-inflammatory effect of alloferon on UVB-induced skin inflammation through the down-regulation of pro-inflammatory cytokines","volume":"149","author":"Kim","year":"2013","journal-title":"Immunol. Lett."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"8379539","DOI":"10.1155\/2017\/8379539","article-title":"Resveratrol-enriched rice attenuates UVB-ROS-induced skin aging via downregulation of inflammatory cascades","volume":"2017","author":"Subedi","year":"2017","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ansary, T.M., Hossain, M.R., Kamiya, K., Komine, M., and Ohtsuki, M. (2021). Inflammatory molecules associated with ultraviolet radiation-mediated skin aging. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22083974"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1007\/s00011-022-01598-8","article-title":"Photoaging: UV radiation-induced inflammation and immunosuppression accelerate the aging process in the skin","volume":"71","author":"Salminen","year":"2022","journal-title":"Inflamm. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5503","DOI":"10.2174\/0929867324666171106164916","article-title":"The impact of ultraviolet radiation on barrier function in human skin: Molecular mechanisms and topical therapeutics","volume":"25","author":"Alhasaniah","year":"2018","journal-title":"Curr. Med. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Lee, H.R., Hong, S.-M., Cho, K., Kim, S.H., Ko, E., Lee, E., Kim, H.J., Jeon, S.Y., Do, S.G., and Kim, S.Y. (2024). Potential Role of Dietary Salmon Nasal Cartilage Proteoglycan on UVB-Induced Photoaged Skin. Biomol. Ther., 32.","DOI":"10.4062\/biomolther.2024.010"},{"key":"ref_32","first-page":"9","article-title":"Oral collagen supplementation: A systematic review of dermatological applications","volume":"18","author":"Choi","year":"2019","journal-title":"J. Drugs Dermatol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Anbualakan, K., Tajul Urus, N.Q., Makpol, S., Jamil, A., Mohd Ramli, E.S., Md Pauzi, S.H., and Muhammad, N. (2022). A scoping review on the effects of carotenoids and flavonoids on skin damage due to ultraviolet radiation. Nutrients, 15.","DOI":"10.3390\/nu15010092"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1111\/ijfs.13345","article-title":"Effects of UV-B on vitamin C, phenolics, flavonoids and their related enzyme activities in mung bean sprouts (Vigna radiata)","volume":"52","author":"Wang","year":"2017","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1089\/ars.2013.5653","article-title":"Damage to skin extracellular matrix induced by UV exposure","volume":"21","author":"Watson","year":"2014","journal-title":"Antioxid. Redox Signal."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.jtv.2016.03.002","article-title":"Introduction to skin aging","volume":"26","author":"Tobin","year":"2017","journal-title":"J. Tissue Viability"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e16688","DOI":"10.1111\/jocd.16688","article-title":"Influences on skin and intrinsic aging: Biological, environmental, and therapeutic insights","volume":"24","author":"Hussein","year":"2025","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1111\/j.1600-0781.1997.tb00214.x","article-title":"Structural and biochemical basis for the UVB-induced alterations in epidermal barrier function","volume":"13","author":"Holleran","year":"1997","journal-title":"Photodermatol. Photoimmunol. Photomed."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1080\/09540105.2021.1967300","article-title":"Fermented black rice and blueberry with Lactobacillus plantarum MG4221 improve UVB-induced skin injury","volume":"32","author":"Park","year":"2021","journal-title":"Food Agric. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s00403-009-0994-y","article-title":"The UV response of the skin: A review of the MAPK, NF\u03baB and TNF\u03b1 signal transduction pathways","volume":"302","author":"Muthusamy","year":"2010","journal-title":"Arch. Dermatol. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1089\/rej.2017.1971","article-title":"Prunella vulgaris L. exerts a protective effect against extrinsic aging through NF-\u03baB, MAPKs, AP-1, and TGF-\u03b2\/Smad signaling pathways in UVB-aged normal human dermal fibroblasts","volume":"21","author":"Zhang","year":"2018","journal-title":"Rejuvenation Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1467-2494.2004.00241.x","article-title":"Ultraviolet radiation and skin aging: Roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation\u2013a review","volume":"27","author":"Pillai","year":"2005","journal-title":"Int. J. Cosmet. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"9","DOI":"10.14310\/horm.2002.11109","article-title":"The human skin as a hormone target and an endocrine gland","volume":"3","author":"Zouboulis","year":"2004","journal-title":"HORMONES-ATHENS-"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Shuster, E. (2008). Hormonal influences in multiple sclerosis. Adv. Mult. Scler. Exp. Demyelinating Dis., 267\u2013311.","DOI":"10.1007\/978-3-540-73677-6_11"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.jdermsci.2007.02.001","article-title":"Increase of collagen synthesis by obovatol through stimulation of the TGF-\u03b2 signaling and inhibition of matrix metalloproteinase in UVB-irradiated human fibroblast","volume":"46","author":"Choi","year":"2007","journal-title":"J. Dermatol. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"253","DOI":"10.4161\/derm.21923","article-title":"Hyaluronic acid: A key molecule in skin aging","volume":"4","author":"Papakonstantinou","year":"2012","journal-title":"Derm.-Endocrinol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Kang, M.C., Yumnam, S., and Kim, S.Y. (2018). Oral intake of collagen peptide attenuates ultraviolet B irradiation-induced skin dehydration in vivo by regulating hyaluronic acid synthesis. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19113551"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e15029","DOI":"10.1111\/exd.15029","article-title":"Cutaneous-immuno-neuro-endocrine (CINE) system: A complex enterprise transforming skin into a super organ","volume":"33","author":"Shastri","year":"2024","journal-title":"Exp. Dermatol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1992","DOI":"10.1210\/en.2017-03230","article-title":"How UV light touches the brain and endocrine system through skin, and why","volume":"159","author":"Slominski","year":"2018","journal-title":"Endocrinology"},{"key":"ref_50","first-page":"17","article-title":"Collagen-structure, properties and application","volume":"23","author":"Owczarzy","year":"2020","journal-title":"Eng. Biomater."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1111\/exd.12832","article-title":"The dynamic anatomy and patterning of skin","volume":"25","author":"Wong","year":"2016","journal-title":"Exp. Dermatol."},{"key":"ref_52","first-page":"47","article-title":"Skin aging","volume":"17","year":"2008","journal-title":"Acta Dermatovenerol. Alp. Panon. Adriat."},{"key":"ref_53","unstructured":"Schoenfeld, P. (2018). The Collagen Diet: Rejuvenate Skin, Strengthen Joints and Feel Younger by Boosting Collagen Intake and Production, Simon and Schuster."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2701","DOI":"10.1080\/13880209.2016.1179768","article-title":"Ethanol extract of Terminalia chebula fruit protects against UVB-induced skin damage","volume":"54","author":"Yakaew","year":"2016","journal-title":"Pharm. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Son, D.J., Jung, J.C., Choi, Y.M., Ryu, H.Y., Lee, S., and Davis, B.A. (2020). Wheat extract oil (WEO) attenuates UVB-induced photoaging via collagen synthesis in human keratinocytes and hairless mice. Nutrients, 12.","DOI":"10.3390\/nu12020300"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Imokawa, G. (2009). Mechanism of UVB-Induced Wrinkling of the Skin: Paracrine Cytokine Linkage Between Keratinocytes and Fibroblasts Leading to the Stimulation of Elastase, Elsevier.","DOI":"10.1038\/jidsymp.2009.11"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Horv\u00e1th, S., Koml\u00f3di, R., Perkecz, A., Pint\u00e9r, E., Gyulai, R., and Kem\u00e9ny, \u00c1. (2019). Methodological refinement of Aldara-induced psoriasiform dermatitis model in mice. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-39903-x"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Neu, S.D., Strzepa, A., Martin, D., Sorci-Thomas, M.G., Pritchard, K.A., and Dittel, B.N. (2021). Myeloperoxidase inhibition ameliorates plaque psoriasis in mice. Antioxidants, 10.","DOI":"10.3390\/antiox10091338"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/13\/6427\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:04:05Z","timestamp":1760033045000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/13\/6427"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,3]]},"references-count":58,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["ijms26136427"],"URL":"https:\/\/doi.org\/10.3390\/ijms26136427","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,3]]}}}