{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T19:25:16Z","timestamp":1789241116153,"version":"build-2803163510"},"reference-count":186,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:00:00Z","timestamp":1751587200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Three-dimensional (3D) spheroid models have revolutionized in vitro cancer research by offering more physiologically relevant alternatives to traditional two-dimensional (2D) cultures. A systematic search identifies English-language studies on patient-derived cancer spheroids for drug screening, using defined inclusion and exclusion criteria, with data extracted on cancer type, culture methods, spheroid characteristics, and therapeutic responses. This manuscript evaluates the methods for spheroid formation and the cellular sources used, highlighting the diverse applications and preferences in this field. The five most investigated cancer origins for spheroid seeding are breast, colon, lung, ovary, and brain cancers, reflecting their clinical importance and research focus. Among seeding methodologies, forced-floating and scaffold-based methods predominate, demonstrating reliability and versatility in spheroid generation. Other techniques, including microfluidics, bioprinting, hanging drop, and suspension culture also play significant roles, each with distinct advantages and limitations. This review underscores the increasing use of spheroid models and the need for standardization in methodologies to enhance the reproducibility and translational potential in cancer research.<\/jats:p>","DOI":"10.3390\/ijms26136478","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T08:58:23Z","timestamp":1751878703000},"page":"6478","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Unlocking the Potential of Spheroids in Personalized Medicine: A Systematic Review of Seeding Methodologies"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1003-5752","authenticated-orcid":false,"given":"Karolina M.","family":"Lonkwic","sequence":"first","affiliation":[{"name":"Clinic of General and Oncological Surgery, Medical University of Lodz, 92-213 Lodz, Poland"},{"name":"Mabion S.A., 95-050 Konstantynow Lodzki, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rados\u0142aw","family":"Zajdel","sequence":"additional","affiliation":[{"name":"Department of Economic and Medical Informatics, University of Lodz, 90-214 Lodz, Poland"},{"name":"Department of Medical Informatics and Statistics, Medical University of Lodz, 90-645 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Kaczka","sequence":"additional","affiliation":[{"name":"Clinic of General and Oncological Surgery, Medical University of Lodz, 92-213 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1002\/jez.1400090405","article-title":"The Outgrowth of the Nerve Fiber as a Mode of Protoplasmic Movement","volume":"9","author":"Harrison","year":"1910","journal-title":"J. Exp. Zool."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1084\/jem.97.5.695","article-title":"Studies on the Propagation in Vitro of Poliomyelitis Viruses. IV. Viral Multiplication in a Stable Strain of Human Malignant Epithelial Cells (Strain HeLa) Derived from an Epidermoid Carcinoma of the Cervix","volume":"97","author":"Scherer","year":"1953","journal-title":"J. Exp. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e2202936","DOI":"10.1002\/adhm.202202936","article-title":"Recent Advances on Cell Culture Platforms for In Vitro Drug Screening and Cell Therapies: From Conventional to Microfluidic Strategies","volume":"12","author":"Cardoso","year":"2023","journal-title":"Adv. Healthc. Mater."},{"key":"ref_4","unstructured":"Moo-Young, M. (2011). 5.42\u2014In Vitro Cancer Model for Drug Testing. Comprehensive Biotechnology, Academic Press. [2nd ed.]."},{"key":"ref_5","first-page":"910","article-title":"2D and 3D Cell Cultures\u2014A Comparison of Different Types of Cancer Cell Cultures","volume":"14","author":"Kolenda","year":"2016","journal-title":"Arch. Med. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1002\/jcp.24683","article-title":"3D Cell Culture Systems: Advantages and Applications","volume":"230","author":"Ravi","year":"2015","journal-title":"J. Cell. Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Zanoni, M., Piccinini, F., Arienti, C., Zamagni, A., Santi, S., Polico, R., Bevilacqua, A., and Tesei, A. (2016). 3D Tumor Spheroid Models for in Vitro Therapeutic Screening: A Systematic Approach to Enhance the Biological Relevance of Data Obtained. Sci. Rep., 6.","DOI":"10.1038\/srep19103"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Ryoo, H., Kimmel, H., Rondo, E., and Underhill, G.H. (2023). Advances in High Throughput Cell Culture Technologies for Therapeutic Screening and Biological Discovery Applications. Bioeng. Transl. Med., 9.","DOI":"10.1002\/btm2.10627"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Hoarau-V\u00e9chot, J., Rafii, A., Touboul, C., and Pasquier, J. (2018). Halfway between 2D and Animal Models: Are 3D Cultures the Ideal Tool to Study Cancer-Microenvironment Interactions?. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19010181"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"45745","DOI":"10.18632\/oncotarget.9935","article-title":"The Relevance of Using 3D Cell Cultures, in Addition to 2D Monolayer Cultures, When Evaluating Breast Cancer Drug Sensitivity and Resistance","volume":"7","author":"Breslin","year":"2016","journal-title":"Oncotarget"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.drudis.2012.10.003","article-title":"Three-Dimensional Cell Culture: The Missing Link in Drug Discovery","volume":"18","author":"Breslin","year":"2013","journal-title":"Drug Discov. Today"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Jensen, C., and Teng, Y. (2020). Is It Time to Start Transitioning From 2D to 3D Cell Culture?. Front. Mol. Biosci., 7.","DOI":"10.3389\/fmolb.2020.00033"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Habanjar, O., Diab-Assaf, M., Caldefie-Chezet, F., and Delort, L. (2021). 3D Cell Culture Systems: Tumor Application, Advantages, and Disadvantages. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222212200"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"110264","DOI":"10.1016\/j.msec.2019.110264","article-title":"Comparison of 2D and 3D Cell Culture Models for Cell Growth, Gene Expression and Drug Resistance","volume":"107","author":"Fontoura","year":"2020","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Abuwatfa, W.H., Pitt, W.G., and Husseini, G.A. (2024). Scaffold-Based 3D Cell Culture Models in Cancer Research. J. Biomed. Sci., 31.","DOI":"10.1186\/s12929-024-00994-y"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Franchi-Mendes, T., Eduardo, R., Domenici, G., and Brito, C. (2021). 3D Cancer Models: Depicting Cellular Crosstalk within the Tumour Microenvironment. Cancers, 13.","DOI":"10.3390\/cancers13184610"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Park, C.H., Park, J.H., and Suh, Y.J. (2024). Perspective of 3D Culture in Medicine: Transforming Disease Research and Therapeutic Applications. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1491669"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"(2003). Goodbye, flat biology?. Nature, 424, 861.","DOI":"10.1038\/424861b"},{"key":"ref_19","first-page":"100334","article-title":"Ultra-Sensitive pH Responsive Hydrogels with Injectable and Self-Healing Performance for Controlled Drug Delivery","volume":"9","author":"Yu","year":"2025","journal-title":"Int. J. Pharm. X"},{"key":"ref_20","first-page":"113","article-title":"Growth of Multicell Spheroids in Tissue Culture as a Model of Nodular Carcinomas","volume":"46","author":"Sutherland","year":"1971","journal-title":"JNCI J. Natl. Cancer Inst."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"El Harane, S., Zidi, B., El Harane, N., Krause, K.-H., Matthes, T., and Preynat-Seauve, O. (2023). Cancer Spheroids and Organoids as Novel Tools for Research and Therapy: State of the Art and Challenges to Guide Precision Medicine. Cells, 12.","DOI":"10.3390\/cells12071001"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Gilazieva, Z., Ponomarev, A., Rutland, C., Rizvanov, A., and Solovyeva, V. (2020). Promising Applications of Tumor Spheroids and Organoids for Personalized Medicine. Cancers, 12.","DOI":"10.3390\/cancers12102727"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Nayak, P., Bentivoglio, V., Varani, M., and Signore, A. (2023). Three-Dimensional In Vitro Tumor Spheroid Models for Evaluation of Anticancer Therapy: Recent Updates. Cancers, 15.","DOI":"10.3390\/cancers15194846"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Agastin, S., Giang, U.-B.T., Geng, Y., DeLouise, L.A., and King, M.R. (2011). Continuously Perfused Microbubble Array for 3D Tumor Spheroid Model. Biomicrofluidics, 5.","DOI":"10.1063\/1.3596530"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1021\/acsbiomaterials.6b00144","article-title":"Rapid Enhancement of Cellular Spheroid Assembly by Acoustically Driven Microcentrifugation","volume":"2","author":"Alhasan","year":"2016","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"6924","DOI":"10.1021\/acsami.9b21471","article-title":"Adjustable and Versatile 3D Tumor Spheroid Culture Platform with Interfacial Elastomeric Wells","volume":"12","author":"An","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1002\/1878-0261.12156","article-title":"Characterization of Genetic Intratumor Heterogeneity in Colorectal Cancer and Matching Patient-Derived Spheroid Cultures","volume":"12","author":"Jeppesen","year":"2018","journal-title":"Mol. Oncol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.2147\/DDDT.S108004","article-title":"Real-Time Monitoring of Cisplatin Cytotoxicity on Three-Dimensional Spheroid Tumor Cells","volume":"10","author":"Baek","year":"2016","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2349","DOI":"10.1002\/1097-0142(19810515)47:10<2349::AID-CNCR2820471005>3.0.CO;2-L","article-title":"Surgical Adjuvant Therapy in Colon Carcinoma: A Human Tumor Spheroid Model for Evaluating Radiation Sensitizing Agents","volume":"47","author":"Barone","year":"1981","journal-title":"Cancer"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1177\/1087057105277841","article-title":"A More Aggressive Breast Cancer Spheroid Model Coupled to an Electronic Capillary Sensor System for a High-Content Screening of Cytotoxic Agents in Cancer Therapy: 3-Dimensional in Vitro Tumor Spheroids as a Screening Model","volume":"10","author":"Impidjati","year":"2005","journal-title":"J. Biomol. Screen."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1097\/JCMA.0000000000000226","article-title":"Phenotypic and microRNA Characterization of the Neglected CD24+ Cell Population in MCF-7 Breast Cancer 3-Dimensional Spheroid Culture","volume":"83","author":"Boo","year":"2020","journal-title":"J. Chin. Med. Assoc."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Brooks, E.A., Gencoglu, M.F., Corbett, D.C., Stevens, K.R., and Peyton, S.R. (2019). An Omentum-Inspired 3D PEG Hydrogel for Identifying ECM-Drivers of Drug Resistant Ovarian Cancer. APL Bioeng., 3.","DOI":"10.1063\/1.5091713"},{"key":"ref_33","first-page":"400","article-title":"Glioblastoma Spheroid Growth and Chemotherapeutic Responses in Single and Dual-Stiffness Hydrogels","volume":"163","author":"Bruns","year":"2023","journal-title":"Mech. Cells Fibers"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1021\/acsabm.1c01138","article-title":"Type-I Collagen\/Collagenase Modulates the 3D Structure and Behavior of Glioblastoma Spheroid Models","volume":"5","author":"Calori","year":"2022","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1002\/elps.202100382","article-title":"Microfluidic Platform for Studying the Anti-Cancer Effect of Ursolic Acid on Tumor Spheroid","volume":"43","author":"Chang","year":"2022","journal-title":"Electrophoresis"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"117","DOI":"10.4236\/jct.2022.133009","article-title":"Breast Cancer MCF-7 Cell Spheroid Culture for Drug Discovery and Development","volume":"13","author":"Chen","year":"2022","journal-title":"J. Cancer Ther."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1007\/s10544-010-9417-2","article-title":"Alginate-Based Microfluidic System for Tumor Spheroid Formation and Anticancer Agent Screening","volume":"12","author":"Chen","year":"2010","journal-title":"Biomed. Microdevices"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Chen, Z., Ma, N., Sun, X., Li, Q., Zeng, Y., Chen, F., Sun, S., Xu, J., Zhang, J., and Ye, H. (2021). Automated Evaluation of Tumor Spheroid Behavior in 3D Culture Using Deep Learning-Based Recognition. Biomaterials, 272.","DOI":"10.1016\/j.biomaterials.2021.120770"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"596","DOI":"10.18632\/oncoscience.171","article-title":"High-Content Analysis of Tumour Cell Invasion in Three-Dimensional Spheroid Assays","volume":"2","author":"Cheng","year":"2015","journal-title":"Oncoscience"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.slasd.2021.10.008","article-title":"Detection and Impact of Hypoxic Regions in Multicellular Tumor Spheroid Cultures Formed by Head and Neck Squamous Cell Carcinoma Cells Lines","volume":"27","author":"Close","year":"2022","journal-title":"SLAS Discov."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Das, T., Meunier, L., Barbe, L., Provencher, D., Guenat, O., Gervais, T., and Mes-Masson, A.-M. (2013). Empirical Chemosensitivity Testing in a Spheroid Model of Ovarian Cancer Using a Microfluidics-Based Multiplex Platform. Biomicrofluidics, 7.","DOI":"10.1063\/1.4774309"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1177\/1087057116651867","article-title":"Reproducibility of Uniform Spheroid Formation in 384-Well Plates: The Effect of Medium Evaporation","volume":"21","author":"Das","year":"2016","journal-title":"SLAS Discov."},{"key":"ref_43","first-page":"e55403","article-title":"Evaporation-Reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates","volume":"121","author":"Das","year":"2017","journal-title":"J. Vis. Exp."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1007\/s10565-018-9423-3","article-title":"Colorectal Cancer Spheroid Biobanks: Multi-Level Approaches to Drug Sensitivity Studies","volume":"34","author":"Bruselles","year":"2018","journal-title":"Cell Biol. Toxicol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"112100","DOI":"10.1016\/j.msec.2021.112100","article-title":"Fabrication of PNIPAm-Based Thermoresponsive Hydrogel Microwell Arrays for Tumor Spheroid Formation","volume":"125","author":"Dhamecha","year":"2021","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"7630","DOI":"10.1039\/C6TB02668K","article-title":"The Effect of the Shape of Gold Core\u2013Mesoporous Silica Shell Nanoparticles on the Cellular Behavior and Tumor Spheroid Penetration","volume":"4","author":"Dias","year":"2016","journal-title":"J. Mater. Chem. B"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Domenici, G., Eduardo, R., Castillo-Ecija, H., Orive, G., Montero Carcaboso, \u00c1., and Brito, C. (2021). PDX-Derived Ewing\u2019s Sarcoma Cells Retain High Viability and Disease Phenotype in Alginate Encapsulated Spheroid Cultures. Cancers, 13.","DOI":"10.3390\/cancers13040879"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Dufau, I., Frongia, C., Sicard, F., Dedieu, L., Cordelier, P., Ausseil, F., Ducommun, B., and Valette, A. (2012). Multicellular Tumor Spheroid Model to Evaluate Spatio-Temporal Dynamics Effect of Chemotherapeutics: Application to the Gemcitabine\/CHK1 Inhibitor Combination in Pancreatic Cancer. BMC Cancer, 12.","DOI":"10.1186\/1471-2407-12-15"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1021\/acs.molpharmaceut.7b00843","article-title":"Ratio-Dependent Synergism of a Doxorubicin and Olaparib Combination in 2D and Spheroid Models of Ovarian Cancer","volume":"15","author":"Eetezadi","year":"2017","journal-title":"Mol. Pharm."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Eguchi, H., Kimura, R., Onuma, S., Ito, A., Yu, Y., Yoshino, Y., Matsunaga, T., Endo, S., and Ikari, A. (2022). Elevation of Anticancer Drug Toxicity by Caffeine in Spheroid Model of Human Lung Adenocarcinoma A549 Cells Mediated by Reduction in Claudin-2 and Nrf2 Expression. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232415447"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1093\/neuonc\/nos266","article-title":"Cyclopamine Cooperates with EGFR Inhibition to Deplete Stem-like Cancer Cells in Glioblastoma-Derived Spheroid Cultures","volume":"14","author":"Eimer","year":"2012","journal-title":"Neuro-Oncology"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"6844","DOI":"10.1364\/BOE.440444","article-title":"Three-Dimensional Dynamics Optical Coherence Tomography for Tumor Spheroid Evaluation","volume":"12","author":"Miyazawa","year":"2021","journal-title":"Biomed. Opt. Express"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1002\/1097-0290(20010320)72:6<579::AID-BIT1023>3.0.CO;2-L","article-title":"Dynamics of Spheroid Self-Assembly in Liquid-Overlay Culture of DU 145 Human Prostate Cancer Cells","volume":"72","author":"Enmon","year":"2001","journal-title":"Biotechnol. Bioeng."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1016\/j.tranon.2019.04.001","article-title":"A Three-Dimensional Ex Vivo Viability Assay Reveals a Strong Correlation Between Response to Targeted Inhibitors and Mutation Status in Melanoma Lymph Node Metastases","volume":"12","author":"McFadden","year":"2019","journal-title":"Transl. Oncol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"7462","DOI":"10.1021\/acsabm.0c00679","article-title":"One-Step Dip-Coating-Fabricated Core\u2013Shell Silk Fibroin Rice Paper Fibrous Scaffolds for 3D Tumor Spheroid Formation","volume":"3","author":"Fu","year":"2020","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.colsurfb.2018.11.038","article-title":"Spontaneous Formation of Tumor Spheroid on a Hydrophilic Filter Paper for Cancer Stem Cell Enrichment","volume":"174","author":"Fu","year":"2019","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1039\/D1LC01012C","article-title":"Acoustic Bubble for Spheroid Trapping, Rotation, and Culture: A Tumor-on-a-Chip Platform (ABSTRACT Platform)","volume":"22","author":"Gao","year":"2022","journal-title":"Lab Chip"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1021\/acsbiomaterials.7b00069","article-title":"Comparative Study of Multicellular Tumor Spheroid Formation Methods and Implications for Drug Screening","volume":"4","author":"Gencoglu","year":"2018","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.18632\/oncotarget.28134","article-title":"Optimization of Tumor Spheroid Model in Mesothelioma and Lung Cancers and Anti-Cancer Drug Testing in H2052\/484 Spheroids","volume":"12","author":"Gendre","year":"2021","journal-title":"Oncotarget"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1186\/s12935-021-01898-9","article-title":"Morphological and Molecular Characteristics of Spheroid Formation in HT-29 and Caco-2 Colorectal Cancer Cell Lines","volume":"21","author":"Gheytanchi","year":"2021","journal-title":"Cancer Cell Int."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Goisnard, A., Daumar, P., Dubois, C., Aubel, C., Roux, M., Depresle, M., Gauthier, J., Vidalinc, B., Penault-Llorca, F., and Mounetou, E. (2021). LightSpot\u00ae-FL-1 Fluorescent Probe: An Innovative Tool for Cancer Drug Resistance Analysis by Direct Detection and Quantification of the P-glycoprotein (P-gp) on Monolayer Culture and Spheroid Triple Negative Breast Cancer Models. Cancers, 13.","DOI":"10.3390\/cancers13164050"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/s00441-018-2920-0","article-title":"Enrichment of Cancer Stem Cells by Agarose Multi-Well Dishes and 3D Spheroid Culture","volume":"375","author":"Guo","year":"2019","journal-title":"Cell Tissue Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.21873\/anticanres.11555","article-title":"Spheroid-Based 3D Cell Cultures Enable Personalized Therapy Testing and Drug Discovery in Head and Neck Cancer","volume":"37","author":"Hagemann","year":"2017","journal-title":"Anticancer Res."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Han, S., Lim, J.Y., Cho, K., Lee, H.W., Park, J.Y., Ro, S.W., Kim, K.S., Seo, H.R., and Kim, D.Y. (2022). Anti-Cancer Effects of YAP Inhibitor (CA3) in Combination with Sorafenib against Hepatocellular Carcinoma (HCC) in Patient-Derived Multicellular Tumor Spheroid Models (MCTS). Cancers, 14.","DOI":"10.3390\/cancers14112733"},{"key":"ref_65","first-page":"e60273","article-title":"Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-Resolution Confocal Microscopy","volume":"151","author":"Harmer","year":"2019","journal-title":"J. Vis. Exp."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s00262-016-1927-1","article-title":"A Novel Three-Dimensional Heterotypic Spheroid Model for the Assessment of the Activity of Cancer Immunotherapy Agents","volume":"66","author":"Herter","year":"2017","journal-title":"Cancer Immunol. Immunother."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Ho, W.Y., Yeap, S.K., Ho, C.L., Rahim, R.A., and Alitheen, N.B. (2012). Development of Multicellular Tumor Spheroid (MCTS) Culture from Breast Cancer Cell and a High Throughput Screening Method Using the MTT Assay. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0044640"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"6355236","DOI":"10.1155\/2021\/6355236","article-title":"Synergistic Cytotoxicity between Elephantopus Scaber and Tamoxifen on MCF-7-Derived Multicellular Tumor Spheroid","volume":"2021","author":"Ho","year":"2021","journal-title":"Evid. -Based Complement. Altern. Med."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"134","DOI":"10.21037\/tcr-21-1577","article-title":"Patient-Derived Tumor Spheroid Cultures as a Promising Tool to Assist Personalized Therapeutic Decisions in Breast Cancer","volume":"11","author":"Hofmann","year":"2021","journal-title":"Transl. Cancer Res."},{"key":"ref_70","first-page":"3093","article-title":"Toxicity of Mitoxantrone-Loaded Superparamagnetic Iron Oxide Nanoparticles in a HT-29 Tumour Spheroid Model","volume":"36","author":"Hornung","year":"2016","journal-title":"Anticancer Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"5395","DOI":"10.2147\/OTT.S249756","article-title":"Characterization of Triple-Negative Breast Cancer MDA-MB-231 Cell Spheroid Model","volume":"13","author":"Huang","year":"2020","journal-title":"OncoTargets Ther."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1124\/jpet.119.256693","article-title":"Cellular Uptake and Efflux of Palbociclib In Vitro in Single Cell and Spheroid Models","volume":"370","author":"Jove","year":"2019","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Ju, F.N., Kim, C.-H., Lee, K.-H., Kim, C.-D., Lim, J., Lee, T., Park, C.G., and Kim, T.-H. (2023). Gold Nanostructure-Integrated Conductive Microwell Arrays for Uniform Cancer Spheroid Formation and Electrochemical Drug Screening. Biosens. Bioelectron., 222.","DOI":"10.1016\/j.bios.2022.115003"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Karamikamkar, S., Behzadfar, E., and Cheung, K.C. (2018). A Novel Approach to Producing Uniform 3-D Tumor Spheroid Constructs Using Ultrasound Treatment. Biomed. Microdevices, 20.","DOI":"10.1007\/s10544-018-0260-1"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1016\/j.yexcr.2012.03.026","article-title":"Loss of Cancer Drug Activity in Colon Cancer HCT-116 Cells during Spheroid Formation in a New 3-D Spheroid Cell Culture System","volume":"318","author":"Karlsson","year":"2012","journal-title":"Exp. Cell Res."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"103202","DOI":"10.1016\/j.pdpdt.2022.103202","article-title":"Antitumor Activity of Photodynamic Therapy with Tetracationic Derivative of Synthetic Bacteriochlorin in Spheroid Culture of Liver and Colon Cancer Cells","volume":"40","author":"Karshieva","year":"2022","journal-title":"Photodiagn. Photodyn. Ther."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Kato, E.E., and Sampaio, S.C. (2021). Crotoxin Modulates Events Involved in Epithelial\u2013Mesenchymal Transition in 3D Spheroid Model. Toxins, 13.","DOI":"10.3390\/toxins13110830"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1901751","DOI":"10.1002\/adhm.201901751","article-title":"Vertically Coated Graphene Oxide Micro-Well Arrays for Highly Efficient Cancer Spheroid Formation and Drug Screening","volume":"9","author":"Kim","year":"2020","journal-title":"Adv. Healthc. Mater."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"2822","DOI":"10.1039\/C9LC00140A","article-title":"Tumor Spheroid-on-a-Chip: A Standardized Microfluidic Culture Platform for Investigating Tumor Angiogenesis","volume":"19","author":"Ko","year":"2019","journal-title":"Lab Chip"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1089\/adt.2018.896","article-title":"High Content Screening Characterization of Head and Neck Squamous Cell Carcinoma Multicellular Tumor Spheroid Cultures Generated in 384-Well Ultra-Low Attachment Plates to Screen for Better Cancer Drug Leads","volume":"17","author":"Kochanek","year":"2019","journal-title":"Assay Drug Dev. Technol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1177\/2472555219896999","article-title":"Maximizing the Value of Cancer Drug Screening in Multicellular Tumor Spheroid Cultures: A Case Study in Five Head and Neck Squamous Cell Carcinoma Cell Lines","volume":"25","author":"Kochanek","year":"2020","journal-title":"SLAS Discov."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1002\/jcb.25247","article-title":"Metabolic Suppression of a Drug-Resistant Subpopulation in Cancer Spheroid Cells","volume":"117","author":"Koshkin","year":"2016","journal-title":"J. Cell. Biochem."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.ejpb.2022.10.003","article-title":"Surprising Efficacy Twist of Two Established Cytostatics Revealed by A-La-Carte 3D Cell Spheroid Preparation Protocol","volume":"180","year":"2022","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Kudl\u00e1\u010dov\u00e1, J., Kotrchov\u00e1, L., Kostka, L., Rand\u00e1rov\u00e1, E., Filipov\u00e1, M., Janou\u0161kov\u00e1, O., Fang, J., and Etrych, T. (2020). Structure-to-Efficacy Relationship of HPMA-Based Nanomedicines: The Tumor Spheroid Penetration Study. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12121242"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"3789","DOI":"10.1021\/acs.molpharmaceut.7b00548","article-title":"Polylactide-Based Block Copolymeric Micelles Loaded with Chlorin E6 for Photodynamic Therapy: In Vitro Evaluation in Monolayer and 3D Spheroid Models","volume":"14","author":"Kumari","year":"2017","journal-title":"Mol. Pharm."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1177\/2472555217698818","article-title":"Exploring Drug Dosing Regimens In Vitro Using Real-Time 3D Spheroid Tumor Growth Assays","volume":"22","author":"McGee","year":"2017","journal-title":"SLAS Discov."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1016\/j.bmc.2012.12.007","article-title":"Development, Validation and Pilot Screening of an in Vitro Multi-Cellular Three-Dimensional Cancer Spheroid Assay for Anti-Cancer Drug testing","volume":"21","author":"Lama","year":"2013","journal-title":"Bioorg. Med. Chem."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Landgraf, L., Kozlowski, A., Zhang, X., Fournelle, M., Becker, F.-J., Tretbar, S., and Melzer, A. (2022). Focused Ultrasound Treatment of a Spheroid In Vitro Tumour Model. Cells, 11.","DOI":"10.3390\/cells11091518"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"540","DOI":"10.3109\/21691401.2014.968820","article-title":"A Collagen-Based Multicellular Tumor Spheroid Model for Evaluation of the Efficiency of Nanoparticle Drug Delivery","volume":"44","author":"Le","year":"2016","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"3041","DOI":"10.1002\/bit.27114","article-title":"In Vitro Lung Cancer Multicellular Tumor Spheroid Formation Using a Microfluidic Device","volume":"116","author":"Lee","year":"2019","journal-title":"Biotechnol. Bioeng."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"e529","DOI":"10.1002\/cpz1.529","article-title":"High-Throughput Screening of Anti-Cancer Drugs Using a Microfluidic Spheroid Culture Device with a Concentration Gradient Generator","volume":"2","author":"Lee","year":"2022","journal-title":"Curr. Protoc."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1007\/s12195-014-0349-4","article-title":"Optimization of Aqueous Biphasic Tumor Spheroid Microtechnology for Anti-Cancer Drug Testing in 3D Culture","volume":"7","author":"Lemmo","year":"2014","journal-title":"Cell. Mol. Bioeng."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"152653","DOI":"10.1016\/j.prp.2019.152653","article-title":"Enhancement of Cisplatin-Induced Cytotoxicity against Cervical Cancer Spheroid Cells by Targeting Long Non-Coding RNAs","volume":"215","author":"Li","year":"2019","journal-title":"Pathol. Res. Pract."},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Lim, W., and Park, S. (2018). A Microfluidic Spheroid Culture Device with a Concentration Gradient Generator for High-Throughput Screening of Drug Efficacy. Molecules, 23.","DOI":"10.20944\/preprints201811.0608.v1"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.1021\/acssensors.0c02266","article-title":"Thermosensitive and Conductive Hybrid Polymer for Real-Time Monitoring of Spheroid Growth and Drug Responses","volume":"6","author":"Lin","year":"2021","journal-title":"ACS Sens."},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Liu, X., Lin, H., Song, J., Zhang, T., Wang, X., Huang, X., and Zheng, C. (2021). A Novel SimpleDrop Chip for 3D Spheroid Formation and Anti-Cancer Drug Assay. Micromachines, 12.","DOI":"10.3390\/mi12060681"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1747-1028-6-22","article-title":"Live Cell Division Dynamics Monitoring in 3D Large Spheroid Tumor Models Using Light Sheet Microscopy","volume":"6","author":"Lorenzo","year":"2011","journal-title":"Cell Div."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"2364","DOI":"10.1039\/D2LC00244B","article-title":"Non-Small Cell Lung Carcinoma Spheroid Models in Agarose Microwells for Drug Response Studies","volume":"22","author":"Luan","year":"2022","journal-title":"Lab Chip"},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Madsen, N.H., Nielsen, B.S., Nhat, S.L., Skov, S., Gad, M., and Larsen, J. (2021). Monocyte Infiltration and Differentiation in 3D Multicellular Spheroid Cancer Models. Pathogens, 10.","DOI":"10.3390\/pathogens10080969"},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Marshall, S.K., Saelim, B., Taweesap, M., Pachana, V., Panrak, Y., Makchuchit, N., and Jaroenpakdee, P. (2022). Anti-EGFR Targeted Multifunctional I-131 Radio-Nanotherapeutic for Treating Osteosarcoma: In Vitro 3D Tumor Spheroid Model. Nanomaterials, 12.","DOI":"10.3390\/nano12193517"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.bbamcr.2017.12.005","article-title":"Elevation of Sensitivity to Anticancer Agents of Human Lung Adenocarcinoma A549 Cells by Knockdown of Claudin-2 Expression in Monolayer and Spheroid Culture Models","volume":"1865","author":"Maruhashi","year":"2018","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"ref_102","doi-asserted-by":"crossref","unstructured":"Melnik, D., Sahana, J., Corydon, T.J., Kopp, S., Nassef, M.Z., Wehland, M., Infanger, M., Grimm, D., and Kr\u00fcger, M. (2020). Dexamethasone Inhibits Spheroid Formation of Thyroid Cancer Cells Exposed to Simulated Microgravity. Cells, 9.","DOI":"10.3390\/cells9020367"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1002\/jbm.b.34832","article-title":"Physically-Cross-Linked Poly(Vinyl Alcohol) Cell Culture Plate Coatings Facilitate Preservation of Cell\u2013Cell Interactions, Spheroid Formation, and Stemness","volume":"109","author":"Molyneaux","year":"2021","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"R45","DOI":"10.1186\/bcr1747","article-title":"Application of the Multicellular Tumour Spheroid Model to Screen PET Tracers for Analysis of Early Response of Chemotherapy in Breast Cancer","volume":"9","author":"Monazzam","year":"2007","journal-title":"Breast Cancer Res."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.1111\/cas.15840","article-title":"Novel and Efficient Method for Culturing Patient-Derived Gastric Cancer Stem Cells","volume":"114","author":"Morimoto","year":"2023","journal-title":"Cancer Sci."},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Mosaad, E.O., Chambers, K.F., Futrega, K., Clements, J.A., and Doran, M.R. (2018). The Microwell-Mesh: A High-Throughput 3D Prostate Cancer Spheroid and Drug-Testing Platform. Sci. Rep., 8.","DOI":"10.1038\/s41598-017-18050-1"},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Mueggler, A., Pilotto, E., Perriraz-Mayer, N., Jiang, S., Addeo, A., B\u00e9dat, B., Karenovics, W., Triponez, F., and Serre-Beinier, V. (2023). An Optimized Method to Culture Human Primary Lung Tumor Cell Spheroids. Cancers, 15.","DOI":"10.21203\/rs.3.rs-3456085\/v1"},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Nashimoto, Y., Okada, R., Hanada, S., Arima, Y., Nishiyama, K., Miura, T., and Yokokawa, R. (2020). Vascularized Cancer on a Chip: The Effect of Perfusion on Growth and Drug Delivery of Tumor Spheroid. Biomaterials, 229.","DOI":"10.1016\/j.biomaterials.2019.119547"},{"key":"ref_109","doi-asserted-by":"crossref","unstructured":"Nigjeh, S.E., Yeap, S.K., Nordin, N., Kamalideghan, B., Ky, H., and Rosli, R. (2018). Citral Induced Apoptosis in MDA-MB-231 Spheroid Cells. BMC Complement. Altern. Med., 18.","DOI":"10.1186\/s12906-018-2115-y"},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Nittayaboon, K., Leetanaporn, K., Sangkhathat, S., Roytrakul, S., and Navakanitworakul, R. (2022). Cytotoxic Effect of Metformin on Butyrate-Resistant PMF-K014 Colorectal Cancer Spheroid cells. Biomed. Pharmacother., 151.","DOI":"10.1016\/j.biopha.2022.113214"},{"key":"ref_111","doi-asserted-by":"crossref","unstructured":"Ohya, S., Kajikuri, J., Endo, K., Kito, H., and Matsui, M. (2021). KCa1.1 K+ Channel Inhibition Overcomes Resistance to Antiandrogens and Doxorubicin in a Human Prostate Cancer LNCaP Spheroid Model. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222413553"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.ijpharm.2016.08.049","article-title":"Solid Lipid Nanoparticles Co-Loaded with Doxorubicin and \u03b1-Tocopherol Succinate Are Effective against Drug-Resistant Cancer Cells in Monolayer and 3-D Spheroid Cancer Cell Models","volume":"512","author":"Oliveira","year":"2016","journal-title":"Int. J. Pharm."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.7150\/ijms.74109","article-title":"Reproduction of the Antitumor Effect of Cisplatin and Cetuximab Using a Three-Dimensional Spheroid Model in Oral Cancer","volume":"19","author":"Ono","year":"2022","journal-title":"Int. J. Med. Sci."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1177\/2472555217696798","article-title":"A Novel Cellular Spheroid-Based Autophagy Screen Applying Live Fluorescence Microscopy Identifies Nonactin as a Strong Inducer of Autophagosomal Turnover","volume":"22","author":"Pampaloni","year":"2017","journal-title":"SLAS Discov."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1158\/0008-5472.CAN-14-2611","article-title":"Novel Morphologic and Genetic Analysis of Cancer Cells in a 3D Microenvironment Identifies STAT3 as a Regulator of Tumor Permeability Barrier Function","volume":"76","author":"Park","year":"2016","journal-title":"Cancer Res."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"2540","DOI":"10.1007\/s11095-016-1978-1","article-title":"Targeting of Micelles and Liposomes Loaded with the Pro-Apoptotic Drug, NCL-240, into NCI\/ADR-RES Cells in a 3D Spheroid Model","volume":"33","author":"Pattni","year":"2016","journal-title":"Pharm. Res."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.jconrel.2012.09.003","article-title":"Accumulation and Toxicity of Antibody-Targeted Doxorubicin-Loaded PEG\u2013PE Micelles in Ovarian Cancer Cell Spheroid Model","volume":"164","author":"Perche","year":"2012","journal-title":"J. Control. Release"},{"key":"ref_118","doi-asserted-by":"crossref","unstructured":"Preda, P., Enciu, A.-M., Tanase, C., Dudau, M., Albulescu, L., Maxim, M.-E., Darie-Ni\u021b\u0103, R.N., Brincoveanu, O., and Avram, M. (2023). Assessing Polysaccharides\/Aloe Vera\u2013Based Hydrogels for Tumor Spheroid Formation. Gels, 9.","DOI":"10.3390\/gels9010051"},{"key":"ref_119","doi-asserted-by":"crossref","unstructured":"Pulze, L., Congiu, T., Brevini, T.A.L., Grimaldi, A., Tettamanti, G., D\u2019Antona, P., Baranzini, N., Acquati, F., Ferraro, F., and de Eguileor, M. (2020). MCF7 Spheroid Development: New Insight about Spatio\/Temporal Arrangements of TNTs, Amyloid Fibrils, Cell Connections, and Cellular Bridges. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21155400"},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"16948","DOI":"10.18632\/oncotarget.7659","article-title":"Comparative Analysis of Tumor Spheroid Generation Techniques for Differential in Vitro Drug Toxicity","volume":"7","author":"Raghavan","year":"2016","journal-title":"Oncotarget"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1186\/s40425-019-0666-1","article-title":"Ovarian Cancer Stem Cells and Macrophages Reciprocally Interact through the WNT Pathway to Promote Pro-Tumoral and Malignant Phenotypes in 3D Engineered Microenvironments","volume":"7","author":"Raghavan","year":"2019","journal-title":"J. Immunother. Cancer"},{"key":"ref_122","doi-asserted-by":"crossref","unstructured":"Ralph, A.C.L., Valad\u00e3o, I.C., Cardoso, E.C., Martins, V.R., Oliveira, L.M.S., Bevilacqua, E.M.A.F., Geraldo, M.V., Jaeger, R.G., Goldberg, G.S., and Freitas, V.M. (2020). Environmental Control of Mammary Carcinoma Cell Expansion by Acidification and Spheroid Formation in Vitro. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-78989-6"},{"key":"ref_123","doi-asserted-by":"crossref","unstructured":"Roering, P., Siddiqui, A., Heuser, V.D., Potdar, S., Mikkonen, P., Oikkonen, J., Li, Y., Pikkusaari, S., Wennerberg, K., and Hynninen, J. (2022). Effects of Wee1 Inhibitor Adavosertib on Patient-Derived High-Grade Serous Ovarian Cancer Cells Are Multiple and Independent of Homologous Recombination Status. Front. Oncol., 12.","DOI":"10.3389\/fonc.2022.954430"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"11843","DOI":"10.1007\/s13277-016-5041-y","article-title":"Evidence for Embryonic Stem-like Signature and Epithelial-Mesenchymal Transition Features in the Spheroid Cells Derived from Lung Adenocarcinoma","volume":"37","author":"Roudi","year":"2016","journal-title":"Tumor Biol."},{"key":"ref_125","first-page":"352","article-title":"Lithium Increases Radiosensitivity by Abrogating DNA Repair in Breast Cancer Spheroid Culture","volume":"17","author":"Rouhani","year":"2014","journal-title":"Arch. Iran. Med."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s11626-017-0207-5","article-title":"Establishment and Proteomic Characterization of Patient-Derived Clear Cell Sarcoma Xenografts and Cell Lines","volume":"54","author":"Sakumoto","year":"2018","journal-title":"In Vitro Cell. Dev. Biol. Anim."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"3645","DOI":"10.1016\/j.ijbiomac.2020.08.084","article-title":"Stabilization of Zataria Essential Oil with Pectin-Based Nanoemulsion for Enhanced Cytotoxicity in Monolayer and Spheroid Drug-Resistant Breast Cancer Cell Cultures and Deciphering Its Binding Mode with gDNA","volume":"164","author":"Salehi","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"1995","DOI":"10.2147\/DDDT.S242514","article-title":"A Triple Combination of Metformin, Acetylsalicylic Acid, and Oseltamivir Phosphate Impacts Tumour Spheroid Viability and Upends Chemoresistance in Triple-Negative Breast Cancer","volume":"14","author":"Sambi","year":"2020","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_129","doi-asserted-by":"crossref","unstructured":"Sankar, S., Mehta, V., Ravi, S., Sharma, C.S., and Rath, S.N. (2021). A Novel Design of Microfluidic Platform for Metronomic Combinatorial Chemotherapy Drug Screening Based on 3D Tumor Spheroid Model. Biomed. Microdevices, 23.","DOI":"10.1007\/s10544-021-00593-w"},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/s41420-021-00812-6","article-title":"Pediatric Multicellular Tumor Spheroid Models Illustrate a Therapeutic Potential by Combining BH3 Mimetics with Natural Killer (NK) Cell-Based Immunotherapy","volume":"8","author":"Shanmugalingam","year":"2022","journal-title":"Cell Death Discov."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"110608","DOI":"10.1016\/j.cellsig.2023.110608","article-title":"EGFR and Lyn Inhibition Augments Regorafenib Induced Cell Death in Sorafenib Resistant 3D Tumor Spheroid Model","volume":"105","author":"Karpat","year":"2023","journal-title":"Cell. Signal."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1093\/carcin\/bgx003","article-title":"Bisphenol A Activates EGFR and ERK Promoting Proliferation, Tumor Spheroid Formation and Resistance to EGFR Pathway Inhibition in Estrogen Receptor-Negative Inflammatory Breast Cancer Cells","volume":"38","author":"Sauer","year":"2017","journal-title":"Carcinogenesis"},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1007\/s12015-016-9664-6","article-title":"Spheroid-Formation (Colonosphere) Assay for in Vitro Assessment and Expansion of Stem Cells in Colon Cancer","volume":"12","author":"Shaheen","year":"2016","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1007\/s10544-014-9863-3","article-title":"Microcavity Substrates Casted from Self-Assembled Microsphere Monolayers for Spheroid Cell Culture","volume":"16","author":"Shen","year":"2014","journal-title":"Biomed. Microdevices"},{"key":"ref_135","first-page":"20180647","article-title":"Electrochemical Mapping of Oxygenation in the Three-Dimensional Multicellular Tumour Hemi-Spheroid","volume":"475","author":"Sheth","year":"2019","journal-title":"Proc. Math. Phys. Eng. Sci."},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1021\/jasms.2c00307","article-title":"Development of Spheroid-FPOP: An In-Cell Protein Footprinting Method for 3D Tumor Spheroids","volume":"34","author":"Shortt","year":"2023","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1002\/jbm.a.36822","article-title":"Three-Dimensional Cryogel Matrix for Spheroid Formation and Anti-Cancer Drug Screening","volume":"108","author":"Singh","year":"2020","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"2002436","DOI":"10.1002\/smll.202002436","article-title":"A Spheroid-Forming Hybrid Gold Nanostructure Platform That Electrochemically Detects Anticancer Effects of Curcumin in a Multicellular Brain Cancer Model","volume":"17","author":"Suhito","year":"2021","journal-title":"Small"},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.ygyno.2017.04.014","article-title":"Ovarian Cancer Spheroid Shrinkage Following Continuous Exposure to Cisplatin Is a Function of Spheroid Diameter","volume":"146","author":"Tanenbaum","year":"2017","journal-title":"Gynecol. Oncol."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"10446","DOI":"10.1021\/acsami.6b15918","article-title":"High Quality Multicellular Tumor Spheroid Induction Platform Based on Anisotropic Magnetic Hydrogel","volume":"9","author":"Tang","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_141","doi-asserted-by":"crossref","unstructured":"Taubenberger, A.V., Girardo, S., Tr\u00e4ber, N., Fischer-Friedrich, E., Kr\u00e4ter, M., Wagner, K., Kurth, T., Richter, I., Haller, B., and Binner, M. (2019). 3D Microenvironment Stiffness Regulates Tumor Spheroid Growth and Mechanics via P21 and ROCK. Adv. Biosyst., 3.","DOI":"10.1002\/adbi.201900128"},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1186\/s12967-024-04988-0","article-title":"CRISPR\/Cas9-Edited ROS1\u2009+\u2009Non-Small Cell Lung Cancer Cell Lines Highlight Differential Drug Sensitivity in 2D vs. 3D Cultures While Reflecting Established Resistance Profiles","volume":"22","author":"Terrones","year":"2024","journal-title":"J. Transl. Med."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.actbio.2016.12.037","article-title":"Mimicking the Tumor Microenvironment to Regulate Macrophage Phenotype and Assessing Chemotherapeutic Efficacy in Embedded Cancer Cell\/Macrophage Spheroid Models","volume":"50","author":"Tevis","year":"2017","journal-title":"Acta Biomater."},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"233","DOI":"10.3390\/cimb45010018","article-title":"Modulation of Spheroid Forming Capacity and TRAIL Sensitivity by KLF4 and Nanog in Gastric Cancer Cells","volume":"45","author":"To","year":"2023","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.biomaterials.2006.08.054","article-title":"A Multicellular Spheroid Array to Realize Spheroid Formation, Culture, and Viability Assay on a Chip","volume":"28","author":"Torisawa","year":"2007","journal-title":"Biomaterials"},{"key":"ref_146","first-page":"2101","article-title":"Chemo-Sensitivity of Two-Dimensional Monolayer and Three-Dimensional Spheroid of Breast Cancer MCF-7 Cells to Daunorubicin, Docetaxel, and Arsenic Disulfide","volume":"38","author":"Uematsu","year":"2018","journal-title":"Anticancer Res."},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1080\/1061186X.2020.1853743","article-title":"Erlotinib Entrapped in Cholesterol-Depleting Cyclodextrin Nanoparticles Shows Improved Antitumoral Efficacy in 3D Spheroid Tumors of the Lung and the Liver","volume":"29","author":"Varan","year":"2021","journal-title":"J. Drug Target."},{"key":"ref_148","doi-asserted-by":"crossref","unstructured":"Vinci, M., Gowan, S., Boxall, F., Patterson, L., Zimmermann, M., Court, W., Lomas, C., Mendiola, M., Hardisson, D., and Eccles, S.A. (2012). Advances in Establishment and Analysis of Three-Dimensional Tumor Spheroid-Based Functional Assays for Target Validation and Drug Evaluation. BMC Biol., 10.","DOI":"10.1186\/1741-7007-10-29"},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1007\/s10529-016-2035-1","article-title":"Three-Dimensional Perfused Tumour Spheroid Model for Anti-Cancer Drug Screening","volume":"38","author":"Wan","year":"2016","journal-title":"Biotechnol. Lett."},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"2449","DOI":"10.1039\/C4AN00015C","article-title":"Mixed Hydrogel Bead-Based Tumor Spheroid Formation and Anticancer Drug Testing","volume":"139","author":"Wang","year":"2014","journal-title":"Analyst"},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.biomaterials.2016.08.041","article-title":"Generation of an in Vitro 3D PDAC Stroma Rich Spheroid Model","volume":"108","author":"Ware","year":"2016","journal-title":"Biomaterials"},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1590\/1414-431X20132647","article-title":"A Spheroid-Based 3-D Culture Model for Pancreatic Cancer Drug Testing, Using the Acid Phosphatase Assay","volume":"46","author":"Wen","year":"2013","journal-title":"Braz. J. Med. Biol. Res."},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.yexcr.2014.01.017","article-title":"3D High-Content Screening for the Identification of Compounds that Target Cells in Dormant Tumor Spheroid Regions","volume":"323","author":"Wenzel","year":"2014","journal-title":"Exp. Cell Res."},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1177\/2472555219880194","article-title":"A 3D Heterotypic Multicellular Tumor Spheroid Assay Platform to Discriminate Drug Effects on Stroma versus Cancer Cells","volume":"25","author":"Weydert","year":"2020","journal-title":"SLAS Discov."},{"key":"ref_155","doi-asserted-by":"crossref","first-page":"5244","DOI":"10.1002\/jcb.27799","article-title":"Microporous Cellulosic Scaffold as a Spheroid Culture System Modulates Chemotherapeutic Responses and Stemness in Hepatocellular Carcinoma","volume":"120","author":"Wu","year":"2019","journal-title":"J. Cell. Biochem."},{"key":"ref_156","doi-asserted-by":"crossref","first-page":"2000056","DOI":"10.1002\/gch2.202000056","article-title":"A Highly Reproducible Micro U-Well Array Plate Facilitating High-Throughput Tumor Spheroid Culture and Drug Assessment","volume":"5","author":"Wu","year":"2021","journal-title":"Glob. Chall."},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1109\/OJEMB.2019.2962801","article-title":"Temozolomide in Combination With NF-\u03baB Inhibitor Significantly Disrupts the Glioblastoma Multiforme Spheroid Formation","volume":"1","author":"Xia","year":"2020","journal-title":"IEEE Open J. Eng. Med. Biol."},{"key":"ref_158","doi-asserted-by":"crossref","unstructured":"Xiong, Q., Liu, T., Ying, Y., Yu, X., Wang, Z., Gao, H., Lin, T., Fan, W., Zhang, Z., and Wei, Q. (2024). Establishment of Bladder Cancer Spheroids and Cultured in Microfluidic Platform for Predicting Drug Response. Bioeng. Transl. Med., 9.","DOI":"10.1002\/btm2.10624"},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.canlet.2021.08.018","article-title":"Integrative Analyses of Gene Expression and Chemosensitivity of Patient-Derived Ovarian Cancer Spheroids Link G6PD-Driven Redox Metabolism to Cisplatin Chemoresistance","volume":"521","author":"Yamawaki","year":"2021","journal-title":"Cancer Lett."},{"key":"ref_160","first-page":"4923","article-title":"Impact of Spheroid Culture on Molecular and Functional Characteristics of Bladder Cancer Cell Lines","volume":"18","author":"Yoshida","year":"2019","journal-title":"Oncol. Lett."},{"key":"ref_161","doi-asserted-by":"crossref","unstructured":"Yu, L., Ni, C., Grist, S.M., Bayly, C., and Cheung, K.C. (2015). Alginate Core-Shell Beads for Simplified Three-Dimensional Tumor Spheroid Culture and Drug Screening. Biomed. Microdevices, 17.","DOI":"10.1007\/s10544-014-9918-5"},{"key":"ref_162","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1039\/D0NR08076D","article-title":"Investigating the Influence of Block Copolymer Micelle Length on Cellular Uptake and Penetration in a Multicellular Tumor Spheroid Model","volume":"13","author":"Yu","year":"2021","journal-title":"Nanoscale"},{"key":"ref_163","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1039\/c2mt20168b","article-title":"Getting to the Core of Platinum Drug Bio-Distributions: The Penetration of Anti-Cancer Platinum Complexes into Spheroid Tumour Models","volume":"4","author":"Zhang","year":"2012","journal-title":"Metallomics"},{"key":"ref_164","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1039\/c2ib20121f","article-title":"The Use of Spectroscopic Imaging and Mapping Techniques in the Characterisation and Study of DLD-1 Cell Spheroid Tumour Models","volume":"4","author":"Zhang","year":"2012","journal-title":"Integr. Biol."},{"key":"ref_165","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1021\/bp050003l","article-title":"Development of an in Vitro Multicellular Tumor Spheroid Model Using Microencapsulation and Its Application in Anticancer Drug Screening and Testing","volume":"21","author":"Zhang","year":"2005","journal-title":"Biotechnol. Prog."},{"key":"ref_166","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1002\/elps.201600417","article-title":"Studies of Anticancer Drug Cytotoxicity Based on Long-Term HepG2 Spheroid Culture in a Microfluidic System","volume":"38","author":"Zuchowska","year":"2017","journal-title":"Electrophoresis"},{"key":"ref_167","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1023\/B:BREA.0000025418.88785.2b","article-title":"Three-Dimensional in Vitro Tissue Culture Models of Breast Cancer\u2014A Review","volume":"85","author":"Kim","year":"2004","journal-title":"Breast Cancer Res. Treat."},{"key":"ref_168","doi-asserted-by":"crossref","unstructured":"Yau, J.N.N., and Adriani, G. (2023). Three-Dimensional Heterotypic Colorectal Cancer Spheroid Models for Evaluation of Drug Response. Front. Oncol., 13.","DOI":"10.3389\/fonc.2023.1148930"},{"key":"ref_169","doi-asserted-by":"crossref","first-page":"409","DOI":"10.37349\/etat.2024.00226","article-title":"Spheroids and Organoids Derived from Colorectal Cancer as Tools for in Vitro Drug Screening","volume":"5","author":"Salleh","year":"2024","journal-title":"Explor. Target. Anti-Tumor Ther."},{"key":"ref_170","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1038\/bjc.1994.47","article-title":"The Selection of Antibodies for Targeted Therapy of Small-Cell Lung Cancer (SCLC) Using a Human Tumour Spheroid Model to Compare the Uptake of Cluster 1 and Cluster W4 Antibodies","volume":"69","author":"Olabiran","year":"1994","journal-title":"Br. J. Cancer"},{"key":"ref_171","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1186\/s12931-024-02791-5","article-title":"Use of Non-Small Cell Lung Cancer Multicellular Tumor Spheroids to Study the Impact of Chemotherapy","volume":"25","author":"Hulo","year":"2024","journal-title":"Respir. Res."},{"key":"ref_172","doi-asserted-by":"crossref","unstructured":"Bassi, G., Panseri, S., Dozio, S.M., Sandri, M., Campodoni, E., Dapporto, M., Sprio, S., Tampieri, A., and Montesi, M. (2020). Scaffold-Based 3D Cellular Models Mimicking the Heterogeneity of Osteosarcoma Stem Cell Niche. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-79448-y"},{"key":"ref_173","doi-asserted-by":"crossref","unstructured":"G\u00f3rnicki, T., Lambrinow, J., Golkar-Narenji, A., Data, K., Domaga\u0142a, D., Niebora, J., Farzaneh, M., Mozdziak, P., Zabel, M., and Antosik, P. (2024). Biomimetic Scaffolds\u2014A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering. Nanomaterials, 14.","DOI":"10.3390\/nano14060531"},{"key":"ref_174","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1089\/ten.tec.2015.0280","article-title":"Generation of Homogenous Three-Dimensional Pancreatic Cancer Cell Spheroids Using an Improved Hanging Drop Technique","volume":"22","author":"Ware","year":"2016","journal-title":"Tissue Eng. Part C Methods"},{"key":"ref_175","doi-asserted-by":"crossref","unstructured":"Bia\u0142kowska, K., Komorowski, P., Bryszewska, M., and Mi\u0142owska, K. (2020). Spheroids as a Type of Three-Dimensional Cell Cultures\u2014Examples of Methods of Preparation and the Most Important Application. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21176225"},{"key":"ref_176","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.4252\/wjsc.v11.i12.1065","article-title":"Three-Dimensional Cell Culture Systems as an in Vitro Platform for Cancer and Stem Cell Modeling","volume":"11","author":"Chaicharoenaudomrung","year":"2019","journal-title":"World J. Stem Cells"},{"key":"ref_177","doi-asserted-by":"crossref","unstructured":"Lewis, N.S., Lewis, E.E., Mullin, M., Wheadon, H., Dalby, M.J., and Berry, C.C. (2017). Magnetically Levitated Mesenchymal Stem Cell Spheroids Cultured with a Collagen Gel Maintain Phenotype and Quiescence. J. Tissue Eng., 8.","DOI":"10.1177\/2041731417704428"},{"key":"ref_178","doi-asserted-by":"crossref","unstructured":"Fattahi, P., Rahimian, A., Slama, M.Q., Gwon, K., Gonzalez-Suarez, A.M., Wolf, J., Baskaran, H., Duffy, C.D., Stybayeva, G., and Peterson, Q.P. (2021). Core\u2013Shell Hydrogel Microcapsules Enable Formation of Human Pluripotent Stem Cell Spheroids and Their Cultivation in a Stirred Bioreactor. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-85786-2"},{"key":"ref_179","doi-asserted-by":"crossref","first-page":"2720","DOI":"10.1002\/smll.201502932","article-title":"Efficient one-step production of microencapsulated hepatocyte spheroids with enhanced functions","volume":"12","author":"Chan","year":"2017","journal-title":"Small"},{"key":"ref_180","first-page":"529","article-title":"Development of 3D Bioprinting: From Printing Methods to Biomedical Applications","volume":"15","author":"Gu","year":"2020","journal-title":"Asian J. Pharm. Sci."},{"key":"ref_181","doi-asserted-by":"crossref","first-page":"4369","DOI":"10.1039\/D2LC00705C","article-title":"Bioprinting-Based Automated Deposition of Single Cancer Cell Spheroids into Oxygen Sensor Microelectrode Wells","volume":"22","author":"Dornhof","year":"2022","journal-title":"Lab Chip"},{"key":"ref_182","doi-asserted-by":"crossref","unstructured":"Boularaoui, S., Al Hussein, G., Khan, K.A., Christoforou, N., and Stefanini, C. (2020). An Overview of Extrusion-Based Bioprinting with a Focus on Induced Shear Stress and Its Effect on Cell Viability. Bioprinting, 20.","DOI":"10.1016\/j.bprint.2020.e00093"},{"key":"ref_183","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1186\/s12967-016-1028-0","article-title":"Recent Advances in Bioprinting Techniques: Approaches, Applications and Future Prospects","volume":"14","author":"Li","year":"2016","journal-title":"J. Transl. Med."},{"key":"ref_184","doi-asserted-by":"crossref","first-page":"10083","DOI":"10.1038\/s41467-024-54504-7","article-title":"High-Throughput Bioprinting of Spheroids for Scalable Tissue Fabrication","volume":"15","author":"Kim","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_185","first-page":"146","article-title":"Recent Advances in Microfluidic Technologies for Organ-on-a-Chip","volume":"117","author":"Tian","year":"2019","journal-title":"Cell Anal. Micronanofluid."},{"key":"ref_186","doi-asserted-by":"crossref","unstructured":"Wongpakham, T., Chungfong, T., Jeamsaksiri, W., Chessadangkul, K., Bhanpattanakul, S., Kallayanathum, W., Tharasanit, T., and Pimpin, A. (2024). Development of Pyramidal Microwells for Enhanced Cell Spheroid Formation in a Cell-on-Chip Microfluidic System for Cardiac Differentiation of Mouse Embryonic Stem Cells. 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