{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T16:55:59Z","timestamp":1783097759293,"version":"3.54.6"},"reference-count":36,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T00:00:00Z","timestamp":1752451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Project of the Provincial Department of Education","award":["JJKH20230627KJ"],"award-info":[{"award-number":["JJKH20230627KJ"]}]},{"name":"Science and Technology Project of the Provincial Department of Education","award":["602021037"],"award-info":[{"award-number":["602021037"]}]},{"name":"Yanbian University Doctoral Initiation Fund","award":["JJKH20230627KJ"],"award-info":[{"award-number":["JJKH20230627KJ"]}]},{"name":"Yanbian University Doctoral Initiation Fund","award":["602021037"],"award-info":[{"award-number":["602021037"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Sanguisorba tenuifolia is a wild plant of the genus Sanguisorba officinalis. This study aimed to investigate the regulatory effect of the dichloromethane fraction of Sanguisorba tenuifolia on LPS-induced inflammatory responses in RAW264.7 cells, thereby providing a new scientific basis for the medicinal development of Sanguisorba tenuifolia. Initially, we used 75% ethanol to crudely extract the roots of Sanguisorba tenuifolia, followed by fractional extraction using dichloromethane (CH2Cl2), ethyl acetate (EtOAc), butanol (BuOH), and distilled water (DW) as solvents. By measuring the inhibitory effects of each fractionated extract on NO production, we determined that the SCE (Dichloromethane fraction of Sanguisorba tenuifolia) exhibited the most potent anti-inflammatory activity, leading to its progression to the next experimental stage. Subsequently, we evaluated the effects of SCE on cell viability and LPS-induced inflammatory cytokine secretion in RAW264.7 cells. A rat model of reflux esophagitis was also used to validate the in vivo anti-inflammatory effects of SCE. Additionally, we utilized UPLC\/MS-MS to identify and analyze the active components of SCE. The results indicated that SCE could effectively inhibit LPS-induced cellular inflammation by modulating the p38\/ERK\/MAPK and NF-\u03baB signaling pathways, and also reduced the damage of the esophageal mucosa in rats with reflux esophagitis. UPLC\/MS-MS analysis of SCE identified 423 compounds, including 12 active ingredients such as triterpenoids, phenols, and steroids. This discovery not only provides scientific support for the potential of Sanguisorba tenuifolia as an anti-inflammatory agent but also lays the groundwork for the development of new therapeutics for the treatment of inflammatory diseases.<\/jats:p>","DOI":"10.3390\/ijms26146732","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T09:45:52Z","timestamp":1752572752000},"page":"6732","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Dichloromethane Fraction of Sanguisorba tenuifolia Inhibits Inflammation in Cells Through Modulation of the p38\/ERK\/MAPK and NF-\u03baB Signaling Pathway"],"prefix":"10.3390","volume":"26","author":[{"given":"Yue","family":"Wang","sequence":"first","affiliation":[{"name":"College of Agronomy, Yanbian University, Yanji 133002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Yanbian University, Yanji 133002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuao","family":"Niu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Yanbian University, Yanji 133002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqi","family":"Fa","sequence":"additional","affiliation":[{"name":"College of Agronomy, Yanbian University, Yanji 133002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Nan","sequence":"additional","affiliation":[{"name":"College of Agronomy, Yanbian University, Yanji 133002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4229-4356","authenticated-orcid":false,"given":"Hyeon Hwa","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Crop Life Sciences, College of Agricultural Life Sciences, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk State, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5276130","DOI":"10.1155\/2016\/5276130","article-title":"Role of Antioxidants and Natural Products in Inflammation","volume":"2016","author":"Arulselvan","year":"2016","journal-title":"Oxid. 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